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백서의 배양 골아세포와 파골세포에 대한 산화적 손상과 Glutamate 수용제 길항제의 영향
박승택,전승호,이병찬 대한동의생리학회,대한동의병리학회 2003 동의생리병리학회지 Vol.17 No.4
It is well known that oxidative stress of reactive oxygen species(ROS) may be a causative factor in the pathogenesis of bone disorder. The purpose of this study was to evaluate the cytotoxicity of oxidative stress. Cell viability by MTS assay or INT assay, activity of glutathione peroxidase(GPx), lipid peroxidation(LPO) activity and cell viability. And also protctive effect of glutamate receptors against ROS-induced osteotoxicity was examined by protein synthesis, alkaline phosphatase (ALP) activity and lactate dehydrogenase (LDH) activity in cultured rat osteoblasts and osteoclasts. XO/HX decreased cell viability and GPx activity, protein synthesis and ALP activity, but increased LPO activity and LDH activity. In the protective effect, N-methyl-D-aspartate (NMDA) receptor antagonists or AMPA/kainate receptor antagonists such as D-2-amino-5-phosphonovaleric acid (APV), 7-chlorokynurenic acid (CKA), 6-cyano-7. nitroqinoxaline-2,3-dior1e (CNQX) and 6,7-dinitroquinoxaline-2,3-dior1e (DNQX), NMDA receptor antagonists but AMPA/kainate receptor antagonists showed protective effect on xanthine oxidase (XO) and hypoxanthine (HX) in these cultures by the increse of protein synthesis, ALP activity.
Adriamycin이 白鼠 培養心筋細胞와 內皮細胞에 미치는 細胞毒性에 관한 硏究
朴承澤,金錤元,鄭然泰 圓光大學校 醫科學硏究所 1988 圓光醫科學 Vol.4 No.1-2
In an attempt to evaluate the cytotoxicity of adriamycin, an anticancer drug, colorimetric assays of neutral red(NR), tetrazolium MTT, lactate dehydrogenase activity and protein content were investigated on myocardial and endothelial cells from the newborn rat heart grown for 96 hours in media containing adriamycin of various concentrations. Light and electron microscopic studies were also carried out on these cells. Initial and midpoint cytotoxicities of adriamycin in myocardial cells were detected at lower concentrations in the NR assay(NR-90, 0.07 ㎍/ml ; NR-50, 2.0㎍/ml) than in the MTT assay (MTT-90, 0.15㎍/ml ; MTT-50, 2.8㎍/ml). However, in endothelial cells, they were noted to be at lower concentrations in the MTT assay(MTT-90, 0.1㎍/ml ; MTT-50, 2.3㎍/ml) than in the NR assay (NR-90, 0.3㎍/ml ; NR-50, 4.1㎍/ml). Protein content in myocardial cells treated with adriamycin, NR-50 and MTT-50 were calculated to be 38.1 % and 34.1 % to the control respectively and these in endothelial cells grown in media containing adriamycin, NR-50 and MTT-50 were 36.4% and 45.7 % to the control respectively. With addition of adriamycin, increase in amount of LDH was noted in media of both myocardial and endothelial cells and was dose-dependent of adriamycin. In light microscopy, both myocardial and endothelial cells were observed to decrease in number and shape of these cells became more spherical than that of the control with adriamycin treatment. Electron microscopy of adriamycin treated cells showed increment of lysosome and vacuoles, swelling of mitochondria and cisternal dilatation of rough endoplasmic reticulum. These results suggest that adriamycin inhibits in vitro proliferation and growth of the cells by disturbing the cell metabolism.
박승택,윤향석,형근영,조정구,이강창,김원신,김형민,전병훈,윤용갑,Park Seung-Taeck,Yoon Hyang-Suk,Hyoung Keon-Young,Cho Chung-Gu,Lee Kang-Chang,Kim Won-Shin,Kim Hyung-Min,Jeon Byung-Hoon,Yun Young-Gap 대한한의학방제학회 1999 大韓韓醫學方劑學會誌 Vol.7 No.1
In order to eludidate the mechanism of oxidative stress in cultured spinal motor neurons damaged by oxygen free radicals, cytoxicity was assesed by MTT assay and NR assay after spinal motor neurons from mouse were cultured in media containing various concentrations of xanthine oxidase(XO) and hypoxanthine(HX) for 3 hours. In addition, neuroprotective effects of several herb extracts on oxidant-induced neurotoxicity were examined in these cultures, compared with nerve growth factors such as basic fibroblast growth factor(bFGF). XO/HX decreased cell viability in dose- and time dependent manners on cultured mouse spinal motor neurons, and MTT50 and NR50 values were measured at 20mU/ml XO and 0.1mM HX for 3 hours in these cultures. bFGF significantlt increased cell viability. In neuroprotective of herb extracts, Epimedium Koreanum Nakai(EK) and Alpinia oxphylla Mig(IJI) was very effective in the prevention of the neurotoxicity induced by XO/HX in cultured mouse spinal motor neurons. From the above results, it is suggested that XO/HX shows toxic effect in cultured mouse spinal motor neurons and selective herb extracts such as Epimedium Koreanum Nakai(EK) and Alpinia oxphylla Mig(IJI) were very effective in the increase of cell viability against the neurotoxicity induced by oxygen radicals in these cultures.
6價크롬의 생쥐의 初期發生段階에 미치는 影響에 關한 形態學的 硏究
朴承澤,金正雄,禹元洪,崔玟圭,鄭然泰 圓光大學校 醫科學硏究所 1987 圓光醫科學 Vol.3 No.1
Mouse two-cell embryos were cultured in the medium containing various concentrations of Cr^(6+) for 72 hours in order to elucidate the cytotoxicity of heavy metal ion and also biochemical observation was performed to measure the protein synthesis in early embryos which were injected with ^3H-leucine for two hours. The results were summerized as follows. 1 - Chromium ion(Cr^(6+)) inhibited remakably the development of mouse early embryos at 1 ppn, when two - cell embryos were cultured for 72 hours at various concentrations of chromium ion. 2. Chromium ion(Cr^(6+)) retarded or suppressed the cleavage of early embryos. Fusion, fragmentation, cytolysis and developmental retardation of blastomeres were observed in the each developmental stage of embryonal cells. 3. Chromium ion(Cr^(6+)) suppressed markedly the incorporation of ^3H-leucine, but didn't give an influence on the uptake of precursor by examing the two-cell embryos exposed to chromium ion for 6 and 24 hours respectively. From this result, it suggest that chromium ion(Cr^(6+)) might suppress the development of early embryos by inhibiting the protein synthesis.