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Li, Zheng,Zhang, Li-Juan,Zhang, Hong-Ru,Tian, Gao-Fei,Tian, Jun,Mao, Xiao-Li,Jia, Zheng-Hu,Meng, Zi-Yu,Zhao, Li-Qing,Yin, Zhi-Nan,Wu, Zhen-Zhou Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.13
Tumors have evolved numerous mechanisms by which they can escape from immune surveillance. One of these is to produce immunosuppressive cytokines. Transforming growth factor-${\beta}$(TGF-${\beta}$) is a pleiotropic cytokine with a crucial function in mediating immune suppression, especially in the tumor microenvironment. TGF-${\beta}$ produced by T cells has been demonstrated as an important factor for suppressing antitumor immune responses, but the role of tumor-derived TGF-${\beta}$ in this process is poorly understood. In this study, we demonstrated that knockdown of tumor-derived TGF-${\beta}$ using shRNA resulted in dramatically reduced tumor size, slowing tumor formation, prolonging survival rate of tumor-bearing mice and inhibiting metastasis. We revealed possible underlying mechanisms as reducing the number of myeloid-derived suppressor cells (MDSC) and $CD4^+Foxp3^+$ Treg cells, and consequently enhanced IFN-${\gamma}$ production by CTLs. Knockdown of tumor-derived TGF-${\beta}$ also significantly reduced the conversion of na$\ddot{i}$ve $CD4^+$ T cells into Treg cells in vitro. Finally, we found that knockdown of TGF-${\beta}$ suppressed cell migration, but did not change the proliferation and apoptosis of tumor cells in vitro. In summary, our study provided evidence that tumor-derived TGF-${\beta}$ is a critical factor for tumor progression and evasion of immune surveillance, and blocking tumor-derived TGF-${\beta}$ may serve as a potential therapeutic approach for cancer.
Ya-Jie Li,Rui Mi,Nan Meng,Zhi-Xin Wen,Xue-Jun Li,Mo Chen,Yan-Qun Liu,Shu-Ying Li 한국응용곤충학회 2013 Journal of Asia-Pacific Entomology Vol.16 No.3
Sans-fille (SNF) is the Drosophila homologue ofmammalian general splicing factors U1A and U2B″, and plays an important role in sex determination in Drosophilamelanogaster. In this study, the snf gene fromAntheraea pernyi (Lepidoptera: Saturniidae), an economically important insect, was isolated and characterized. The obtained 925 bp cDNA sequence contains an open reading frame of 669 bp encoding a polypeptide of 222 amino acids,showing 78% sequence identity to that from D. melanogaster. A database search revealed that SNF protein homologs are present in many animals, including invertebrates and vertebrates, with more than 70% amino acid sequence identities, suggesting that they were highly conserved during the evolution of animals. Phylogenetic analysis revealed that A. pernyi SNF was closely related to Bombyx mori SNF. Quantitative real-time PCR (qRT-PCR) analysis showed that the A. pernyi snf gene was transcribed during five larval developmental stages,and in six tested tissues (ovaries, testes, silk glands, fat body, integument, and hemolymph),with the most abundance determined in the gonads (ovaries or testes). Investigation of expression changes throughout embryonic development indicated that A. pernyi snfmRNAwas expressed at a lowlevel fromdays 0 to 4, and reached amaximum level at day 10, but decreased to a low level before hatching. These results suggest that the product of the snf gene may play important roles in the development of A. pernyi.
Roles of Fibroblast Growth Factor-inducible 14 in Hepatocellular Carcinoma
Li, Nan,Hu, Wen-Jun,Shi, Jie,Xue, Jie,Guo, Wei-Xing,Zhang, Yang,Guan, Dong-Xian,Liu, Shu-Peng,Cheng, Yu-Qiang,Wu, Meng-Chao,Xie, Dong,Liu, Shan-Rong,Cheng, Shu-Qun Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.6
The prognostic value of the fibroblast growth factor-inducible 14 (Fn14) expression in hepatocellular carcinoma (HCC) is unknown. Real-time PCR (RT-PCR), western blot assays and immunohistochemistry analysis were here performed in order to compare Fn14 expressios in paired liver samples of HCC and normal liver tissue. Most of the tumor tissues expressed significantly higher levels of Fn14 compared to adjacent non-tumor tissues, with Fn14High accounting for 54.6% (142/260) of all patients. The Pearson ${\chi}^2$ test indicated that Fn14 expression was closely associated with serum alpha fetal protein (AFP) (P=0.002) and tumor number (p=0.019). Univariate and multivariate analyses revealed that along with tumor diameter and portal vein tumor thrombosis (PVTT ) type, Fn14 was an independent prognostic factor for both overall survival (OS) (HR=1.398, p=0.008) and recurrence (HR=1.541, p=0.001) rates. Fn14 overexpression HCC correlated with poor surgical outcome, and this molecule may be a candidate biomarker for prognosis as well as a target for therapy.
Li-qun Yang,Yong-kuo Liu,Min-jun Peng,Meng-kun Li,Nan Chao 한국원자력학회 2019 Nuclear Engineering and Technology Vol.51 No.5
A fast gamma-ray dose rate assessment method for complex geometries based on stylized modelreconstruction and point-kernel method is proposed in this paper. The complex three-dimensional (3D)geometries are imported as a 3DS format file from 3dsMax software with material and radiometric attributes. Based on 3D stylized model reconstruction of solid mesh, the 3D-geometrical solids are automaticallyconverted into stylized models. In point-kernel calculation, the stylized source models aredivided into point kernels and the mean free paths (mfp) are calculated by the intersections betweenshield stylized models and tracing ray. Compared with MCNP, the proposed method can implementcomplex 3D geometries visually, and the dose rate calculation is accurate and fast.
Fanyu Meng,Shuming Shi,Boshi Zhang,Yunxia Li,Nan Lin 한국자동차공학회 2022 International journal of automotive technology Vol.23 No.6
As more and more researchers begin to analyse the dynamics characteristics of high-degree-of-freedom and nonlinear vehicle models, it is very important and valuable to propose a new analysis method for this type of model. Moreover, it is a promising research direction to propose quantitative indicators for analysing global vehicle dynamics. Therefore, on the basis of classic works, this paper proposes and verifies a quantitative analysis method - dissipation of energy method for 5-DOF nonlinear vehicle plane motion model for the first time. The quantitative indicators for vehicle nonlinear dynamics is expanded. The transfer relationship between the energy components is revealed, which shows that the dissipation of energy method can reflect the dynamics characteristics and stable region characteristics of the nonlinear 5-DOF vehicle system. The effects of tire force lateral-longitudinal coupling and driving modes on global dynamics are analysed. Finally, the prospect of this method is discussed.
Wang, Li-Meng,Xie, Kun-Peng,Huo, Hong-Nan,Shang, Fei,Zou, Wei,Xie, Ming-Jie Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.4
The growth of many breast tumors is stimulated by IGF-1, which activates signal transduction pathways inducing cell proliferation. $ER{\alpha}$ is important in this process. The aim of the study was to investigate relationships in vitro among inhibitory effects of luteolin on the growth of MCF-7 cells, IGF-1 pathway and $ER{\alpha}$. Our results showed that luteolin could effectively block IGF-l-stimulated MCF-7 cell proliferation in a dose- and time-dependent manner and block cell cycle progression and induce apoptosis evidenced by the flow cytometric detection of sub-G1DNA content. Luteolin markedly decreased IGF-l-dependent IGF-IR and Akt phosphorylation without affecting Erk1/2 phosphorylation. Further experiments pointed out that $ER{\alpha}$ was directly involved in IGF-l induced cell growth inhibitory effects of luteolin, which significantly decreased $ER{\alpha}$ expression. Knockdown of $ER{\alpha}$ in MCF-7 cells by an $ER{\alpha}$-specific siRNA decreased the IGF-l induced cell growth inhibitory effects of luteolin. $ER{\alpha}$ is thus a possible target of luteolin. These findings indicate that the inhibitory effect of luteolin on the growth of MCF-7 cells is via inhibiting IGF-l mediated PI3K-Akt pathway dependent of $ER{\alpha}$ expression.
Ya-Nan Zhang,Ji-FangMa,Lu Xu,Zhi-PingDong,Ji-Wei Xu,Meng-Ya Li,Xiu-Yun Zhu 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.1
UDP-glucuronosyltransferase (UGT) genes,which belong to an ancient gene family and play very important roles in all organisms, encode extracellularly secreted proteins that are involved in the transfer of glycosyl residues fromactivated nucleotide sugars to acceptor hydrophobicmolecules (aglycones). Athetis lepigone is an important polyphagous pest worldwide, and since 2011 it has become one of the major maize pests in North China. However, there have been no studies on pesticides for the effective control of this pest. In this study,we identified 23 putative UGT genes in A. lepigone by analysing previous antennal transcriptomic data. Phylogenetic analysis showed that all of the AlUGTs are distributed within 11 of 14 insect UGT sub-families. Tissue expression analysis revealed that N70% of AlUGTs were primarily expressed in adult antennae, of which three (AlUGT33AD1, AlUGT40F6 and AlUGT40L4) and four (AlUGT33B18, AlUGT33F10, AlUGT40Q3 and AlUGT41D3) displayed male-biased and female-biased expression, respectively. SomeAlUGTs, however, had higher expression levels in non-antennal tissues. Our study is the first to identify UGT genes in A. lepigone,which will help us to elucidate the diverse functions of these genes, and ultimately provide potential targets that will facilitate the development of efficient and environmentally friendly pesticides against A. lepigone.
Hai-Nan Lan,Hai-Long Jiang,Wei Li,Tian-Cheng Wu,Pan Hong,Yu Meng Li,Hui Zhang,Huan-Zhong Cui,Xin Zheng 아세아·태평양축산학회 2015 Animal Bioscience Vol.28 No.4
B-32 is one of a panel of monoclonal anti-idiotypic antibodies to growth hormone (GH) that we developed. To characterize and identify its potential role as a novel growth hormone receptor (GHR) agonist, we determined that B-32 behaved as a typical Ab2β based on a series of enzyme-linked immunosorbent assay assays. The results of fluorescence-activated cell sorting, indirect immunofluorescence and competitive receptor binding assays demonstrated that B-32 specifically binds to the GHR expressed on target cells. Next, we examined the resulting signal transduction pathways triggered by this antibody in primary porcine hepatocytes. We found that B-32 can activate the GHR and Janus kinase (2)/signal transducers and activators of transcription (JAK2/STAT5) signalling pathways. The phosphorylation kinetics of JAK2/STAT5 induced by either GH or B-32 were analysed in dose-response and time course experiments. In addition, B32 could also stimulate porcine hepatocytes to secrete insulin-like growth factors-1. Our work indicates that a monoclonal anti-idiotypic antibody to GH (B-32) can serve as a GHR agonist or GH mimic and has application potential in domestic animal (pig) production.