Evaluation on Phytotoxicity of Citrus Peel Extracts to Weeds

Xue-Hua An,Xue-Ping Zhao,Qiang Wang,Xiao-Feng Ji,Chang-Xing Wu 한국잡초학회 2011 한국잡초학회 별책(학술대회 초록집) Vol.31 No.2

Henriciella marina gen. nov., sp. nov., a Novel Member of the Family Hyphomonadaceae Isolated from the East Sea

Zhe-Xue Quan,Dan-Ning Zeng,Yi-Ping Xiao,노성운,Young-Do Nam,Ho-Won Chang,윤정훈,오희목,배진우 한국미생물학회 2009 The journal of microbiology Vol.47 No.2

A bacterial strain, designated Iso4T, was isolated from the East Sea of Korea and was subjected to a polyphasictaxonomy study including phenotypic and chemotaxonomic characteristics as well as 16S rRNAgene sequence analysis. Cells of the strain were Gram-negative, motile, non-budding, non-stalked, andstrictly aerobic. Strain Iso4T grew optimally at 20°C in the presence of 1~2% (w/v) NaCl and at pH 6.9~7.6. The major respiratory quinone was Q-10 and the major cellular fatty acids were C18:1 ω7c (53.5%),C17:1 ω5c (11.7%), C17:1 ω6c (8.1%), C16:0 (7.8%), C17:0 (4.8%), C15:0 (2.9%), and C16:1 ω5c (2.2%). The DNAG+C content of strain Iso4T was 56.2 mol%. Phylogenetic analysis based on 16S rRNA gene sequencesshowed that strain Iso4T formed a monophyletic clade in the family Hyphomonadaceae, supported by highbootstrap value and was most closely related to the genus Hyphomonas (92~94%), a member of marinebacteria in the family. The phenotypic, genotypic, and chemotaxonomic evidences also suggest strain Iso4Trepresents a novel genus and species in the family Hyphomonadaceae, for which the name Henriciella gen. nov., sp. nov. is proposed. The type strain is Iso4T (=KCTC 12513T =DSM 19595T =JCM 15116T).

Susceptibility of Lung Cancer with Polymorphisms of CYP1A1, GSTM1, GSTM3, GSTT1 and GSTP1 Genotypes in the Population of Inner Mongolia Region

Jiang, Xue-Yan,Chang, Fu-Hou,Bai, Tu-Ya,Lv, Xiao-Li,Wang, Min-Jie,Wang, Guang Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.13

Background: To study the relationship of susceptibility to lung cancer with the gene polymorphisms of CYP1A1, GSTM1, GSTM3, GSTT1, GSTP1 and smoking status in Han and Mongolian populations of Inner Mongolia, an autonomous region of China. Materials and Methods: PCR-RFLP, allele-specific and multiplex PCR were employed to identify the genotypes of CYP1A1, GSTM1, GSTM3, GSTT1 and GSTP1 in a case-control study of 322 lung cancer patients diagnosed by bronchoscopy and 456 controls free of malignancy. Results: There is a significant difference in genotypic frequency of GSTT1 of healthy Mongolian and Han subjects. A statistically prominent association was found between CYP1A1 Msp1 (vt/vt) (OR=4.055, 95%CI:2.107-7.578, p=0.000), GSTM1 (-) (OR=2.290, 95%CI:1.467-3.573, p=0.000) and lung cancer in Mongolians. Similarly, in the Han population, CYP1A1 Msp1 (vt/vt) (OR=3.194, 95%CI:1.893-5.390, p=0.000) and GSTM1 (-) (OR=1.884, 95%CI:1.284-2.762, p=0.001) carriers also had an elevated risk of lung cancer. The smokers were more susceptible to lung cancer 2.144 fold and 1.631 fold than non-smokers in Mongolian and Han populations, respectively. The smokers who carried with CYP1A1 Msp1 (wt/vt+vt/vt), exon7 (Val/Val+Ile /Val), GSTM1 (-), GSTM3 (AB+BB), and GSTT1 (-) respectively were found all to have a high risk of lung cancer. Conclusions: CYP1A1 Msp1 (vt/vt) and GSTM1 (-) are risk factors of lung cancer in Han and Mongolian population in the Inner Mongolia region. The smokers with CYP1A1 Msp1 (wt/vt+vt/vt), CYP1A1 exon7 (Val/Val+Ile /Val), GSTM1 (-), GSTM3 (AB+BB), and GSTT1 (-) genotypes, respectively, are at elevated risk of lung cancer.

Posttranscriptional deregulation of Src due to aberrant miR34a and miR203 contributes to gastric cancer development

Qiang Hao,Xiao Zhao Lu,Nan Nan Liu,Xiao Chang Xue,Meng Li,Cun Zhang,Xin Qin,Wei Na Li,Zhen Shu,Bin Song,Qing Wang,Li Qiang Song,Wei Zhang,Ying Qi Zhang 생화학분자생물학회(구 한국생화학분자생물학회) 2013 BMB Reports Vol.46 No.6

Gene Therapy for Mice Sarcoma with Oncolytic Herpes Simplex Virus-1 Lacking the Apoptosis-inhibiting Gene, icp34.5

( Ping Lan ),( Chang Yuan Dong ),( Yi Peng Qi ),( Geng Fu Xiao ),( Feng Xue ) 생화학분자생물학회 2003 BMB Reports Vol.36 No.4

A mutant herpes simplex virus 1, mtHSV, was constructed by inserting the E. coli beta-galactosidase gene into the loci of icp34.5, the apoptosis-inhibiting gene of HSV. The mtHSV replicated in and lysed U251 (human glioma cells), EJ (human bladder cells), and S-180 (mice sarcoma cells), but not Wish (human amnion cells) cells. With its intact tk (thymidine kinase) hene, mtHSV exhibited susceptibility to acyclovir (ACV), which provided an approach to control viral replication. An in vivo test with mtHSV was conducted in immune-competent mice bearing sarcoma S-180 tumors, which were treated with a single intratumoral injection of mtHSV or PBS. Tumor dimensions then were measured at serial time points, and the tumor volumes were calculated. Sarcoma growth was significantly inhibited with prolonged time and reduced tumor volume. There was microscopic evidence of necrosis of tumors in treated mice, whereas no damage was found in other organs. Immunohistochemical staining revealed that virus replication was exclusively confined to the treated tumor cells. HSV-1 DNA was detected in tumors, but not in the other organs by a polymerase chain reaction analysis. From these experiments, we concluded that mtHSV should be a safe and promising oncolytic agent for cancer treatment.

Ni/La2O3-ZrO2 catalyst for hydrogen production from steam reforming of acetic acid as a model compound of bio-oil

Ya-ping Xue,Chang-feng Yan,Xiao-yong Zhao,Shi-lin Huang,Chang-qing Guo 한국화학공학회 2017 Korean Journal of Chemical Engineering Vol.34 No.2

Hydrogen production from steam reforming of acetic acid was investigated over Ni/La2O3-ZrO2 catalyst. A series of Ni/La2O3-ZrO2 catalysts were synthesized by sol-gel method coupled with wet impregnation, which was characterized by XRD, BET, TEM, EDS, TG, SEM and TPR. Catalytic activity of Ni/La2O3-ZrO2 was evaluated by steam reforming of acetic acid at the temperature range of 550-750 oC. The tetragonal phase La0.1Zr0.9O1.95 is formed through the doping of La2O3 into the ZrO2 lattice and nickel species are highly dispersed on the support with high specific surface area. H2 yield and CO2 yield of Ni/La2O3-ZrO2 catalyst with 15%wt Ni reaches 89.27% and 80.41% at 600 oC, respectively, which is attributed to high BET surface area and sufficient Ni active sites in strong interaction with the support. 15%wt Ni supported on La2O3-ZrO2 catalyst maintains relatively stable catalytic activities for a period of 20 h.

Back-fill Grout Experimental Test for Discharged Soils Reuse of the Large-diameter Size Slurry Shield Tunnel

Shunhua Zhou,Xue-Li Zhou,Chang Ji,Junhua Xiao 대한토목학회 2017 KSCE JOURNAL OF CIVIL ENGINEERING Vol.21 No.3

The construction of a slurry shield tunnel produces large amount of excavated soil. Direct discharge of the excavated soil by transportation not only increases the overall construction cost but also raises environmental concerns of pollution. In this study, we explore in-situ recycle of excavated soil in the Nanjing Yangtze River tunnel project as the back-fill grout. A series of laboratory were carried out to determine the synchronous grout parameters, including compression strength and shear strength, initial setting time, fluidity, consistency, and bleeding rate. The mixture ratio of the back-fill grout is also discussed to optimize the grout performance. It is found that: (1) the excavated soil produced from the construction of slurry shield tunnel in this project can be reused as the material of back-fill grout in the sand stratum; (2) Proper selection of binder/sand ratio and fly ash/cement ratio improves the performance of the synchronous grout. The test results demonstrated that the discharged soil can be recycled in-situ as the grouting material in the sand stratum.

Tube Voltage, DNA Double-Strand Breaks, and Image Quality in Coronary CT Angiography

Lin Zhu Xiao,Zhou Fan,Schoepf U. Joseph,Pillai Balakrishnan,Zhou Chang Sheng,Quan Wei,Bao Xue Qin,Lu Guang Ming,Zhang Long Jiang 대한영상의학회 2020 Korean Journal of Radiology Vol.21 No.8

Objective: To evaluate the effects of tube voltage on image quality in coronary CT angiography (CCTA), the estimated radiation dose, and DNA double-strand breaks (DSBs) in peripheral blood lymphocytes to optimize the use of CCTA in the era of low radiation doses. Materials and Methods: This study included 240 patients who were divided into 2 groups according to the DNA DSB analysis methods, i.e., immunofluorescence microscopy and flow cytometry. Each group was subdivided into 4 subgroups: those receiving CCTA only with different tube voltages of 120, 100, 80, or 70 kVp. Objective and subjective image quality was evaluated by analysis of variance. Radiation dosages were also recorded and compared. Results: There was no significant difference in demographic characteristics between the 2 groups and 4 subgroups in each group (all p > 0.05). As tube voltage decreased, both image quality and radiation dose decreased gradually and significantly. After CCTA, γ-H2AX foci and mean fluorescence intensity in the 120-, 100-, 80-, and 70-kVp groups increased by 0.14, 0.09, 0.07, and 0.06 foci per cell and 21.26, 9.13, 8.10, and 7.13 (all p < 0.05), respectively. The increase in the DNA DSB level in the 120-kVp group was higher than those in the other 3 groups (all p < 0.05), while there was no significant difference in the DSBs levels among these latter groups (all p > 0.05). Conclusion: The 100-kVp tube voltage may be optimal for CCTA when weighing DNA DSBs against the estimated radiation dose and image quality, with further reductions in tube voltage being unnecessary for CCTA.

Effects of the Hippo Signaling Pathway in Human Gastric Cancer

Zhou, Guang-Xi,Li, Xiao-Yu,Zhang, Qi,Zhao, Kun,Zhang, Cui-Ping,Xue, Chang-Hu,Yang, Kun,Tian, Zi-Bin Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.9

Background/Aim: The Hippo signaling pathway is a newly discovered and conserved signaling cascade, which regulates organ size control by governing cell proliferation and apoptosis. This study aimed to investigate its effects in human gastric cancer. Methods: Tumor tissues (n=60), adjacent non-tumor tissues (n=60) and normal tissues (n=60) were obtained from the same patients with primary gastric cancer (GC). In addition, 70 samples of chronic atrophic gastritis (CAG) tissues were obtained from patients with intestinal metaplasia (IM) by endoscopic biopsy. Hippo signaling molecules, including Mst1, Lats1, YAP1, TAZ, TEAD1, Oct4 and CDX2, were determined by quantitative polymerase chain reaction (qPCR). Protein expression of Mst1, Lats1, YAP1, TEAD1 and CDX2 was assessed by immunohistochemistry and Western blotting. Results: Mst1, Lats1 and Oct4 mRNA expression showed an increasing tendency from GC tissues to normal gastric tissues, while the mRNA expression of YAP1, TAZ and TEAD1 was up-regulated (all P<0.01). Mst1 and Lats1 protein expression presented a similar trend with their mRNA expression. In addition, YAP1 and TEAD1 protein expression in GC was significantly higher than in the other groups (all P<0.01). CDX2 mRNA and protein expression in the CAG group were higher than in the other groups (all P<0.01). In GC, mRNA expression of Mst1, Lats1, Oct4, YAP1, TAZ, TEAD1 and CDX2 had a close correlation with lymphatic metastasis and tumor TNM stage (all P<0.01). Furthermore, protein expression of Mst1, Lats1, YAP1, TAZ, TEAD1 and CDX2 had a close correlation between each other (P<0.05). Conclusion: The Hippo signaling pathway is involved in the development, progression and metastasis of human gastric cancer. Therefore, manipulation of Hippo signaling molecules may be a potential therapeutic strategy for gastric cancer.

Human Embryonic Stem Cells - a Potential Vaccine for Ovarian Cancer

Zhang, Zu-Juan,Chen, Xin-Hua,Chang, Xiao-Hong,Ye, Xue,Li, Yi,Cui, Heng Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.9

Objective: To investigate the therapeutic potential of human embryonic stem cells (hESCs) as a vaccine to induce an immune response and provide antitumor protection in a rat model. Methods: Cross-reactivity of antigens between hESCs and tumour cells was screened by immunohistochemistry. Fischer 344 rats were divided into 7 groups, with 6 rats in each, immunized with: Group 1, hESC; Group 2, pre-inactivated mitotic NuTu-19; Group 3 PBS; Group 4, hESC; Group 5, pre-inactivated mitotic NuTu-19; Group 6, PBS; Group 7, hESC only. At 1 (Groups 1-3) or 4 weeks (Groups 4-6) after the last vaccination, each rat was challenged intraperitoneally with NuTu-19. Tumor growth and animal survival were closely monitored. Rats immunized with H9 and NuTu-19 were tested by Western blot analysis of rat orbital venous blood for cytokines produced by Th1 and Th2 cells. Results: hESCs presented tumour antigens, markers, and genes related to tumour growth, metastasis, and signal pathway interactions. The vaccine administered to rats in Group 1 led to significant antitumor responses and enhanced tumor rejection in rats with intraperitoneal inoculation of NuTu-19 cells compared to control groups. In contrast, rats in Group 4 did not display any elevation of antitumour responses. Western blot analysis found cross-reactivity among antibodies generated between H9 and NuTu-19. However, the cytokines did not show significant differences, and no side effects were detected. Conclusion: hESC-based vaccination is a promising modality for immunotherapy of ovarian cancer.