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Overexpressing osa-miR171c decreases salt stress tolerance in rice
Wu Yang,Tian Fan,Xiaoying Hu,Taihui Cheng,Mingyong Zhang 한국식물학회 2017 Journal of Plant Biology Vol.60 No.5
The miRNA171 family is one of the well-conservedmiRNA families, and its role under stresses is not knownexcept its expression on genome-wide expression analyses. osa-miR171c was induced by high concentration of salt (150mM NaCl). A rice dh mutant with osa-miR171c overexpressiontriggered by a T-DNA insertion, significantly decreased salttolerance at the stages of germination and seedling. Thisphenotype was confirmed by osa-miR171c overexpressiontransgenic rice. Compared with wild-type (WT), dh mutantreduced amounts of free proline and increased the water lossrate after salt treatment. Stomatal density in the leafepidermis of dh mutant also increased. Moreover, dh mutantincreased sensitivity to ABA treatment. Several stressresponsivegenes were down-regulated in dh mutant than inWT under salty stress. These results indicate that osamiR171cis involved in modulating physiological changes,stomatal development, ABA-dependent pathways and expressionof stress-related genes; thereby, it possibly contributes tosalty tolerance.
Qing-Hua Tian,He-Fei Liu,Tao Wang,Ying-Sheng Cheng,Chun-Gen Wu 대한영상의학회 2019 Korean Journal of Radiology Vol.20 No.6
Objective: To report our initial experience of percutaneous sacroplasty (PSP) with an interpedicular approach for treating painful sacral metastases involving multiple sacral vertebral bodies. Materials and Methods: This study prospectively enrolled 10 consecutive patients (six men and four women; mean age, 56.3 ± 13.8 years) who underwent PSP for painful sacral metastases involving multiple sacral vertebral bodies from March 2017 to September 2018. Visual analogue scale (VAS) scores, Oswestry disability index (ODI) values, and the number of opioids prescribed to the patients were assessed before and after PSP. The procedure duration, length of hospitalization, and complications were also recorded. Results: Mean VAS and ODI declined significantly from 6.90 ± 1.20 and 74.40 ± 5.48 before the procedure to 2.70 ± 1.34 and 29.60 ± 14.57 after the procedure, respectively (p < 0.01). The median number of opioids prescribed per patient decreased from 2 (interquartile range [IQR] 1–3) pre-procedure to 1 (IQR 0–3) post-procedure (p < 0.01). Nine of the 10 patients showed no or decreased opioid usage, and only 1 patient showed unchanged usage. The mean procedure duration was 48.5 ± 3.0 minutes. The average length of hospitalization was 4.7 ± 1.7 days. Extraosseous cement leakage occurred in three cases without causing any clinical complications. Conclusion: PSP with an interpedicular approach is a safe and effective treatment in patients with painful sacral metastases involving multiple sacral vertebral bodies and can relieve pain and improve mobility.
Qing-Hua Tian,Chun-Gen Wu,Quan-Ping Xiao,Cheng-Jian He,Yi-Feng Gu,Tao Wang,Ming-Hua Li 대한영상의학회 2014 Korean Journal of Radiology Vol.15 No.6
Glucocorticosteroid-induced osteoporosis is the most frequent of all secondary types of osteoporosis, and can increase the risk of vertebral compression fractures (VCFs). There are promising additions to current medical treatment for appropriately selected osteoporotic patients. Few studies have reported on the efficiency of percutaneous vertebroplasty (PVP) or kyphoplasty for whole thoracic and lumbar glucocorticosteroid-induced osteoporotic vertebral compression fractures. We report a case of a 67-year-old man with intractable pain caused by successional VCFs treated by PVP.
Li, Yao-Ping,Tian, Fu-Guo,Shi, Peng-Cheng,Guo, Ling-Yun,Wu, Hai-Ming,Chen, Run-Qi,Xue, Jin-Ming Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.23
4-Hydroxynonenal (4-HNE) is a stable end product of lipid peroxidation, which has been shown to play an important role in cell signal transduction, while increasing cell growth and differentiation. 4-HNE could inhibit phosphatase and tensin homolog (PTEN) activity in hepatocytes and increased levels have been found in human invasive breast cancer. Here we report that 4-HNE increased the cell growth of breast cancer cells as revealed by colony formation assay. Moreover, vascular endothelial growth factor (VEGF) expression was elevated, while protein levels of hypoxia inducible factor 1 alpha (HIF-$1{\alpha}$) were up-regulated. Sirtuin-3 (SIRT3), a major mitochondria NAD+-dependent deacetylase, is reported to destabilize HIF-$1{\alpha}$. Here, 4-HNE could inhibit the deacetylase activity of SIRT3 by thiol-specific modification. We further demonstrated that the regulation by 4-HNE of levels of HIF-$1{\alpha}$ and VEGF depends on SIRT3. Consistent with this, 4-HNE could not increase the cell growth in SIRT3 knockdown breast cancer cells. Additionally, 4-HNE promoted angiogenesis and invasion of breast cancer cells in a SIRT3-dependent manner. In conclusion, we propose that 4-HNE promotes growth, invasion and angiogenesis of breast cancer cells through the SIRT3-HIF-$1{\alpha}$-VEGF axis.
Jie Chen,Chun-Xiang Tian,Miao Yu,Qing Lv,Nan-Sheng Cheng,Zu Wang,Xi Wu 한국유방암학회 2014 Journal of breast cancer Vol.17 No.1
Purpose: The aim of the study was to evaluate the efficacy andsafety of combining sorafenib with chemotherapy in patients withhuman epidermal growth factor receptor 2 (HER2)-negative advancedbreast cancer. Methods: MEDLINE, EMBASE, CochraneCentral Register of Controlled Trials, American Society for ClinicalOncology abstracts, and European Society for Medical Oncologyabstracts were searched. Randomized clinical trials that comparedthe efficacy and safety of sorafenib plus chemotherapy inpatients with HER2-negative advanced breast cancer with placeboplus chemotherapy were eligible. The endpoints were progression-free survival (PFS), overall survival (OS), time to progression(TTP), duration of response (DOR), overall response rate(ORR), clinical benefits, and adverse effects. The meta-analysiswas performed using Review Manager 5.2.6 (The Nordic CochraneCentre), and the fixed-effect model weighted by the Mantel-Haenszel method was used. When considerable heterogeneitywas found (p<0.1), further analysis (subgroup analysis, sensitivityanalysis, or random-effect model) was performed to identifythe potential cause. The results are expressed as hazard ratios orrisk ratios, with their corresponding 95% confidence intervals. Results: The final analysis included four trials comprising 844 patients. The results revealed longer PFS and TTP, and higher ORRand clinical benefit rates in patients receiving sorafenib combinedwith chemotherapy compared to those receiving chemotherapyand placebo. OS and DOR were similar in the two groups. Meanwhile,the incidence of some adverse effects, including hand-footskin reaction/hand-foot syndrome, diarrhea, rash, and hypertension,were significantly higher in the sorafenib arm. Conclusion:Sorafenib combined with chemotherapy may prolong PFS andTTP. This treatment was associated with manageable toxicities,but frequent dose interruptions and reductions were required.
Lin Shuang-Zhe,Wu Wei-Jie,Cheng Yu-Qing,Zhang Jian-Bin,Jiang Dai-Xi,Ren Tian-Yi,Ding Wen-Jin,Liu Mingxi,Chen Yuan-Wen,Fan Jian-Gao 생화학분자생물학회 2023 Experimental and molecular medicine Vol.55 No.-
Macrophages are immune cells crucial for host defense and homeostasis maintenance, and their dysregulation is involved in multiple pathological conditions, such as liver fibrosis. The transcriptional regulation in macrophage is indispensable for fine-tuning of macrophage functions, but the details have not been fully elucidated. Prolyl endopeptidase (PREP) is a dipeptidyl peptidase with both proteolytic and non-proteolytic functions. In this study, we found that Prep knockout significantly contributed to transcriptomic alterations in quiescent and M1/M2-polarized bone marrow-derived macrophages (BMDMs), as well as aggravated fibrosis in an experimental nonalcoholic steatohepatitis (NASH) model. Mechanistically, PREP predominantly localized to the macrophage nuclei and functioned as a transcriptional coregulator. Using CUT&Tag and co-immunoprecipitation, we found that PREP was mainly distributed in active cis-regulatory genomic regions and physically interacted with the transcription factor PU.1. Among PREP-regulated downstream genes, genes encoding profibrotic cathepsin B and D were overexpressed in BMDMs and fibrotic liver tissue. Our results indicate that PREP in macrophages functions as a transcriptional coregulator that finely tunes macrophage functions, and plays a protective role against liver fibrosis pathogenesis.
Yong Zhang,Zhuang Zhou,Cheng’ai Wu,Danhui Zhao,Chao Wang,Xiaoguang Cheng,Wei Cai,Ling Wang,Yangyang Duanmu,Chenxin Zhang,Wei Tian 대한영상의학회 2016 Korean Journal of Radiology Vol.17 No.5
Objective: To investigate the bone mineral density (BMD) of cervical vertebrae in a population-stratified manner and correlate with that of the lumbar vertebrae. Materials and Methods: Five hundred and ninety-eight healthy volunteers (254 males, 344 females), ranging from 20 to 64 years of age, were recruited for volumetric BMD (vBMD) measurements by quantitative computed tomography. Basic information (age, height, weight, waistline, and hipline), and vBMD of the cervical and lumbar vertebrae (C2–7 and L2–4) were recorded. Comparisons among sex, age groups and different levels of vertebrae were analyzed using analysis of variance. Linear regression was performed for relevance of different vertebral levels. Results: The vBMD of cervical and lumbar vertebrae was higher in females than males in each age group. The vBMD of the cervical and lumbar vertebrae in males and the vBMD of lumbar vertebrae in females decreased with aging. In each age group, the vBMD of the cervical vertebrae was higher than that of the lumbar vertebrae with gradual decreases from C2 to C7 except for C3; moreover, the vBMD of C6 and C7 was significantly different from that of C2–5. Correlations of vBMD among different cervical vertebrae (females: r = 0.62–0.94; males: r = 0.63–0.94) and lumbar vertebrae (males: r = 0.93–0.98; females: r = 0.82–0.97) were statistically significant at each age group. Conclusion: The present study provided normative data of cervical vertebrae in an age- and sex-stratified manner. Sex differences in vBMD prominently vary with age, which can be helpful to design a more comprehensive pre-operative surgical plan.
Facile Synthesis of CdTe Nanorods from the Growth of Te Nanorods
Xu, Weiwei,Niu, Jinzhong,Zheng, Shuang,Tian, Guimin,Wu, Xinghui,Cheng, Yongguang,Hu, Xiaoyang,Liu, Shuaishuai,Hao, Haoshan Korean Chemical Society 2017 대한화학회지 Vol.61 No.4
One-dimensional CdTe nanorods (NRs) are obtained by the reaction of various Cd precursors with single crystalline Te nanorod templates, which are pre-synthesized from Te precursors by a simple and reproducible solvothermal method. Throughout the process, the diffraction intensity of different crystal facets of single crystalline Te NRs varied with reaction times. Finally, by alloying Cd ions along the axial direction of Te NRs, polycrystalline cubic phase CdTe NRs with diameters of 80-150 nm and length up to $1.2-2.4{\mu}m$ are obtained. The nucleation and growth processes of Te and CdTe NRs are discussed in details, and their properties are characterized by XRD, SEM, TEM, Raman scattering, and UV-vis absorption spectra. It was found that the key elements of synthesizing CdTe NRs such as reaction temperatures and Cd sources will strongly influence the final shape of CdTe NRs.