Evaluation of proteomic strategies for analyzing ubiquitinated proteins

Jun Min Peng 한국생화학분자생물학회 2008 BMB Reports Vol.41 No.3

An intelligent hybrid methodology of on-line system-level fault diagnosis for nuclear power plant

Min-jun Peng,Hang Wang,Shan-shan Chen,Genglei Xia,Yong-kuo Liu,Xu Yang,Abiodun Ayodeji 한국원자력학회 2018 Nuclear Engineering and Technology Vol.50 No.3

To assist operators to properly assess the current situation of the plant, accurate fault diagnosis methodologyshould be available and used. A reliable fault diagnosis method is beneficial for the safety ofnuclear power plants. The major idea proposed in this work is integrating the merits of different faultdiagnosis methodologies to offset their obvious disadvantages and enhance the accuracy and credibilityof on-line fault diagnosis. This methodology uses the principle component analysis-based model andmulti-flow model to diagnose fault type. To ensure the accuracy of results from the multi-flow model, amechanical simulation model is implemented to do the quantitative calculation. More significantly,mechanism simulation is implemented to provide training data with fault signatures. Furthermore, oneof the distance formulas in similarity measurementdMahalanobis distancedis applied for on-line failuredegree evaluation. The performance of this methodology was evaluated by applying it to the reactorcoolant system of a pressurized water reactor. The results of simulation analysis show the effectivenessand accuracy of this methodology, leading to better confidence of it being integrated as a part of thecomputerized operator support system to assist operators in decision-making.

Balanced Reactive-Deliberative Architecture for Multi-Agent System for Simulation League of RoboCup

Min Wu,Jun Peng,Jin-Hua She,Xin Chen,Wei-Hua Cao 제어·로봇·시스템학회 2009 International Journal of Control, Automation, and Vol.7 No.6

This paper presents an architecture for a multi-agent system for the RoboCup simulation league. It consists of a dynamic dual behavior-based architecture for an intelligent agent, a behavior-based decision algorithm, and a dynamic role-based multi-agent cooperation model. A new concept called confidence function is introduced to balance reactivity and deliberation. This architecture was implemented in a team, and match results demonstrate its validity.

A20 ameliorates disc degeneration by suppressing mTOR/BNIP3 axis-mediated mitophagy

Peng Xin,Zhang Cong,Gao Jia-Wei,Wang Feng,Bao Jun-Ping,Zhou Zhi-Min,Sun Rui,Ji Hang-Yu,VLF Cabral,Wu Xiao-Tao 한국유전학회 2023 Genes & Genomics Vol.45 No.5

Background The pathological mechanism of intervertebral disc degeneration (IDD) is an unanswered question that we are committed to exploring. A20 is an anti-inflammatory protein of nucleus pulposus (NP) cells and plays a protective role in intervertebral disc degeneration. Objective This study aims to investigate the molecular mechanism by which A20 attenuates disc degeneration. Methods The proteins of interest were measured by immunoblotting, immunofluorescence, ELISA assay, and immunohistochemical technique to conduct related experiments. Immunofluorescence assays and mitochondrial membrane potential (JC-1) were used to assess mitophagy and mitochondrial fitness, respectively. Results Here, we demonstrated that A20 promoted mitophagy, attenuated pyroptosis, and inhibited the degradation of the extracellular matrix, consequently significantly ameliorating disc degeneration. Mechanistically, A20 reduces pyroptosis and further suppresses cellular mTOR activity. On the one hand, A20-induced mTOR inhibition triggers BNIP3-mediated mitophagy to ensure mitochondrial fitness under LPS stimulation, as a result of mitigating mitochondrial dysfunction induced by LPS. On the other hand, A20-induced mTOR inhibition reduces the loss of mitochondrial membrane potential and the generation of Mitochondrial ROS. Conclusion The study revealed that A20 promotes BNIP3-mediated mitophagy by suppressing mTOR pathway activation against LPS-induced pyroptosis.

Clinical Impact of Drug Adherence of Tyrosine Kinase Inhibitors in Children with Ph-Positive Acute Lymphoblastic Leukemia

Jun-Xia Wang,Miao-Miao Yang,Li-Peng Liu,Hui-Min Zhang,Meng-Chuan Wang,Yu-Wen Chen,Xiao-Ying Zang,Fang Hu 대한암학회 2023 Cancer Research and Treatment Vol.55 No.3

Purpose This study aimed to explore the impact of ABL1–tyrosine kinase inhibitors (TKIs) adherence on the survival of chromosome-positive (Ph+) acute lymphoblastic leukemia (ALL) children and clarify the potential predictors of patients’ prognosis from TKIs intake practices.Materials and Methods Ninety newly diagnosed Ph+ ALL patients who received TKIs were enrolled. We collected the baseline characteristics and adverse events in all children; moreover, TKIs adherence was measured by an eight-item Morisky medication adherence scale (MMAS-8). Progression-free survival (PFS) and overall survival (OS) analysis were performed, and risk factors for PFS and OS were evaluated.Results Among all patients, 69 cases were regarded as adherers, while 21 were non-adherers. The median duration of TKIs interruption was significantly prolonged in the non-adherence group than in the adherence group (13 [0-101] vs. 56 [11-128], p < 0.001). Additionally, dose reduction occurred in 55.2% of non-adherers versus 23.0% of adherers (p=0.002). The PFS and OS in adherers were significantly higher versus non-adherers (p=0.020 and p=0.039). MMAS-8 score was an independent risk factor for PFS (p=0.010) and OS (p=0.031). Among non-adherers, the median OS was only 23.1% (4.2%-42%) in patients aged ≤ 10 years versus 54.4% (38.8%-70%) in adolescents. Most of the patients who experienced TKIs non-adherence suffered pancytopenia.Conclusion TKIs adherence during treatment significantly influenced the survival of pediatric Ph+ ALL patients, and non-adherers with age ≤ 10 years were more vulnerable to TKIs disruption. The cumulative TKIs dose should be especially emphasized to patients with age ≤ 10 years, which may result in an inferior achievement of relevant treatment milestones.

The healing effect of platelet-rich plasma on xenograft in peri-implant bone defects in rabbits

Peng, Wang,Kim, Il-kyu,Cho, Hyun-young,Seo, Ji-Hoon,Lee, Dong-Hwan,Jang, Jun-Min,Park, Seung-Hoon Korean Association of Maxillofacial Plastic and Re 2016 Maxillofacial Plastic Reconstructive Surgery Vol.38 No.-

Background: The association of biomaterial combined with repair factor-like platelet-rich plasma (PRP) has prospective values. Bovine-derived xenograft has been identified as an osteoconductive and biocompatible grafting material that provides osseointegration ability. PRP has become a valuable adjunctive agent to promote healing in a lot of dental and oral surgery procedures. However, there are controversies with respect to the regenerative capacity of PRP and the real benefits of its use in bone grafts. The purpose of this study was to assess the influence of PRP combined with xenograft for the repair of peri-implant bone defects. Methods: Twelve rabbits were used in this study, and the experimental surgery with implant installation was performed simultaneously. Autologous PRP was prepared before the surgical procedure. An intrabony defect (7.0 mm in diameter and 3.0 mm deep) was created in the tibia of each rabbit; then, 24 titanium dental implants (3.0 mm in diameter and 8.5 mm long) were inserted into these osteotomy sites. Thus, a standardized gap (4.0 mm) was established between the surrounding bony walls and the implant surface. The gaps were treated with either xenograft alone (control group) or xenograft combined with PRP (experimental group). After healing for 1, 2, 3, 4, 5, and 6 weeks, the rabbits were sacrificed with an overdose of KCl solution. Two rabbits were killed at each time, and the samples including dental implants and surrounding bone were collected and processed for histological analysis. Results: More newly formed bone and a better bone healing process were observed in control group. The histomorphometric analysis revealed that the mean percentage of bone-to-implant contact in the control group was significantly higher than that of the experimental group (25.23 vs. 8.16 %; P < 0.05, independent-simple t test, analysis of variance [ANOVA]). Conclusions: The results indicate that in the addition of PRP to bovine-derived xenograft in the repair of bone defects around the implant, PRP may delay peri-implant bone healing.

Risk Factors for Anxiety in Major Depressive Disorder Patients

Li-Min Xin,Lin Chen,Zhen-Peng Ji,Suo-Yuan Zhang,Jun Wang,Yan-Hong Liu,Da-Fang Chen,Fu-De Yang,Gang Wang,Yi-Ru Fang,Zheng Lu,Hai-Chen Yang,Jian Hu,Zhi-Yu Chen,Yi Huang,Jing Sun,Xiao-Ping Wang,Hui-Chun 대한정신약물학회 2015 CLINICAL PSYCHOPHARMACOLOGY AND NEUROSCIENCE Vol.13 No.3

Objective: To analyze the sociodemographic and clinical factors related to anxiety in patients with major depressive disorder (MDD). Methods: This study involved a secondary analysis of data obtained from the Diagnostic Assessment Service for People with Bipolar Disorders in China (DASP), which was initiated by the Chinese Society of Psychiatry (CSP) and conducted from September 1, 2010 to February 28, 2011. Based on the presence or absence of anxiety-related characteristics, 1,178 MDD patients were classified as suffering from anxious depression (n=915) or non-anxious depression (n=263), respectively. Results: Compared with the non-anxious group, the anxious-depression group had an older age at onset (t=−4.39, p<0.001), were older (t=−4.69, p<0.001), reported more lifetime depressive episodes (z=−3.24, p=0.001), were more likely to experience seasonal depressive episodes (χ2=6.896, p=0.009) and depressive episodes following stressful life events (χ2=59.350, p <0.001), and were more likely to have a family history of psychiatric disorders (χ2=6.091, p=0.014). Their positive and total scores on the Mood Disorder Questionnaire (MDQ) and the 32-item Hypomania Checklist (HCL-32) (p<0.05) were also lower. The logistic regression analysis indicated that age (odds ratio [OR]=1.03, p<0.001), a lower total MDQ score (OR=0.94, p=0.011), depressive episodes following stressful life events (OR=3.04, p<0.001), and seasonal depressive episodes (OR=1.75, p=0.039) were significantly associated with anxious depression. Conclusion: These findings indicate that older age, fewer subclinical bipolar features, an increased number of depressive episodes following stressful life events, and seasonal depressive episodes may be risk factors for anxiety-related characteristics in patients with MDD.

Could Tumor Size Be A Predictor for Papillary Thyroid Microcarcinoma: a Retrospective Cohort Study

Wang, Min,Wu, Wei-Dong,Chen, Gui-Ming,Chou, Sheng-Long,Dai, Xue-Ming,Xu, Jun-Ming,Peng, Zhi-Hai Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.18

Background: Central lymph node metastasis(CLNM) is common in papillary thyroid microcarcinoma (PTMC). The aim of this study was to define the pathohistologic risk grading based on surgical outcomes. Materials and Methods: Statistical analysis was performed to figure out the optimal cut-off values of size in preoperative ultrasound images for defining the risk of CLNM in papillary thyroid microcarcinoma. Receiver operating characteristic curves (ROC) studies were carried out to determine the cutoff value(s) for the predictor(s). All the patients were divided into two groups according to the above size and the clinic-pathological and immunohistochemical parameters were compared to determine the significance of findings. Results: The optimal cut-off value of tumor size to predict the risk of CLNM in papillary thyroid microcarcinoma was 0.575 cm (area under the curve 0.721) according to the ROC curves. Significant differences were observed on the multifocality, extrathyroidal extension and central lymph node metastasis between two groups which were divided according to the tumor size by the cutoff values. Patients in two groups showed different positive rate and intensity of Ki67. Conclusions: The size of PTMC in ultrasound images are helpful to predict the aggressiveness of the tumors, it could be an easy predictor for PTMC prognosis and assist us to choose treatment.

축구선수들의 경기력 향상을 위한 트레이닝 방법 문헌고찰

이서민 ( Rui-min Li ),조영현 ( Yeong-hyun Cho ),유주인 ( Joo-in Yu ),유붕 ( Peng Liu ),사공혁 ( Hyuk Sakong ),박영준 ( Young-jun Park ),한누리 ( Nu-ri Han ),서태범 ( Tae-beom Seo ) 제주대학교 해양스포츠센터 2020 해양스포츠연구 Vol.10 No.2

본 연구의 목적은 축구선수의 경기력 향상을 위한 트레이닝 방법을 확인하고자 하는데 있다. 이를 위해 온라인(Online) 상에서 Pubmed, Riss, Kiss, Google scholar에서 2000~2020년 사이의 기간을 설정하고, 용어는 Training, Performance, soccer player, core training 그리고 Plyometric training을 사용하여 문헌 조사를 실시하였다. 축구선수의 경기력관련 체력요인 향상을 위한 훈련 프로그램의 효과를 보고한 20개의 논문을 선정하였고, 플라이오메트릭과 코어트레이닝 각각 5개씩 10개의 논문이 최종 채택되었다. 채택한 문헌을 기반으로 내용을 정리한 결과 플라이오메트릭과 코어트레이닝은 근력, 근지구력, 민첩성, 순발력, 허리와 무릎 등속성 근기능 및 유연성을 증가시키는 것으로 확인되었다. 따라서 플라이오메트릭과 코어트레이닝의 적용은 축구선수의 경기력 관련 체력을 향상시킨다는 중요한 정보를 제공한다. The purpose of this study is to identify a training method for improving soccer player's performance. To this end, the period between 2000 and 2020 was set in Pubmed, Riss, Kiss, and Google scholar on Online, and a literature survey was conducted using the terms Training, Performance, soccer player, core training and Plyometric training. Twenty academic journals were selected to report the effectiveness of training programs to improve the fitness factors related to the performance of soccer players, and ten papers were finally selected, five each for plyometric and core training. As a result of organizing the contents based on the adopted literature, it was confirmed that plyometric and core training increased muscle strength, muscular endurance, agility, power, waist and knee isokinetic muscle function and flexibility. Therefore, these results provide information that plyometric and core training may improve physical fitness related to the performance of soccer players.

Network Analyses of Gene Expression following Fascin Knockdown in Esophageal Squamous Cell Carcinoma Cells

Du, Ze-Peng,Wu, Bing-Li,Xie, Jian-Jun,Lin, Xuan-Hao,Qiu, Xiao-Yang,Zhan, Xiao-Fen,Wang, Shao-Hong,Shen, Jin-Hui,Li, En-Min,Xu, Li-Yan Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.13

Fascin-1 (FSCN1) is an actin-bundling protein that induces cell membrane protrusions, increases cell motility, and is overexpressed in various human epithelial cancers, including esophageal squamous cell carcinoma (ESCC). We analyzed various protein-protein interactions (PPI) of differentially-expressed genes (DEGs), in fascin knockdown ESCC cells, to explore the role of fascin overexpression. The node-degree distributions indicated these PPI sub-networks to be characterized as scale-free. Subcellular localization analysis revealed DEGs to interact with other proteins directly or indirectly, distributed in multiple layers of extracellular membrane-cytoskeleton/ cytoplasm-nucleus. The functional annotation map revealed hundreds of significant gene ontology (GO) terms, especially those associated with cytoskeleton organization of FSCN1. The Random Walk with Restart algorithm was applied to identify the prioritizations of these DEGs when considering their relationship with FSCN1. These analyses based on PPI network have greatly expanded our comprehension of the mRNA expression profile following fascin knockdown to future examine the roles and mechanisms of fascin action.