Molecular identification and expression patterns of carboxylesterase genes based on transcriptome analysis of the common cutworm, Spodoptera litura (Lepidoptera: Noctuidae)

Ya Nan Zhang,Jin-Bu Li,Peng He,Liang Sun,Zhao-Qun Li,Li-Ping Fang,Zhan-Feng Ye,Dao-Gui Deng,Xiu-Yun Zhu 한국응용곤충학회 2016 Journal of Asia-Pacific Entomology Vol.19 No.4

Carboxylesterases (CXEs) belong to a family of metabolic enzymes that are widely distributed in insects and other organisms and can rapidly degrade the components of sex pheromones and plant volatiles with an acetate functional group. The common cutworm, Spodoptera litura, is an important agricultural pest around the world, causing vast economic losses every year. The female sex pheromones of S. litura comprise four acetates, Z9, E11-14:OAc; Z9, E12-14:OAc; Z9-14:OAc; and E11-14:OAc, but the degradation mechanisms of these components are not well understood. By analysing previously obtained transcriptomic data of the sex pheromone glands,we identified a total of 24 putative CXE genes in S. litura. Gene expression patterns and phylogenetic analysis revealed 5 genes with antennae-specific or biased expression, and clusteredwith genes showed involvement in the degradation of sex pheromones or other detoxification in other insects. SlitCXE10was expressed specifically in the antennae of both sexes, and SlitCXE14, 17, 19, and 21 had high antenna biased expression. Interestingly, RT-PCR and qPCR tests indicated that SlitCXE24 had significantly higher expression in PG than in other tissue, and that it could be a potential candidate gene for sex pheromone degradation in PG. This study is the first to provide solid background information for the further elucidation of sex pheromone degradation, and ultimately provides potential targets for the disruption of sexual communication in S. litura for new pest management.

De novo assembly and characterization of antennal transcriptome reveal chemosensory system in Nysius ericae

Ya-Nan Zhang,Xiu-Yun Zhu,Qian Zhang,Cai-Yun Yin,Zhi-PingDong,Ling-Hua Zuo,Dao-Gui Deng,Liang Sun,Xiao-Ming Li 한국응용곤충학회 2016 Journal of Asia-Pacific Entomology Vol.19 No.4

Chemosensory system plays important roles in insect behaviors, some key genes governing chemosensation can potentially be used as novel targets for pest control. Nysius ericae (Hemiptera: Lygaeidae) is a polyphagous insect pest found throughout Eurasia countries which causes damage to many important crops. Little is known about the chemosensory mechanisms of host location and mate choice in N. ericae at themolecular level. Here, we assembled the antennal transcriptome of N. ericae using Illumina sequencing technology. Analysis of the transcriptome data identified 148 candidate chemosensory genes, including 28 for odorant binding proteins (OBPs), 16 for chemosensory proteins (CSPs), 2 for sensory neuron membrane proteins (SNMPs), 83 odorant receptors (ORs), 12 ionotropic receptors (IRs), and 7 gustatory receptors (GRs). Our results greatly improve the gene inventory for N. ericae and provide valuable resources for analyzing the functions of some key genes. This information will aid in the development of better understanding of chemosensation in N. ericae, and indicate novel targets for managing its impact on agriculture. Chemosensory system plays important roles in insect behaviors, some key genes governing chemosensation can potentially be used as novel targets for pest control. Nysius ericae (Hemiptera: Lygaeidae) is a polyphagous insect pest found throughout Eurasia countries which causes damage to many important crops. Little is known about the chemosensory mechanisms of host location and mate choice in N. ericae at themolecular level. Here, we assembled the antennal transcriptome of N. ericae using Illumina sequencing technology. Analysis of the transcriptome data identified 148 candidate chemosensory genes, including 28 for odorant binding proteins (OBPs), 16 for chemosensory proteins (CSPs), 2 for sensory neuron membrane proteins (SNMPs), 83 odorant receptors (ORs), 12 ionotropic receptors (IRs), and 7 gustatory receptors (GRs). Our results greatly improve the gene inventory for N. ericae and provide valuable resources for analyzing the functions of some key genes. This information will aid in the development of better understanding of chemosensation in N. ericae, and indicate novel targets for managing its impact on agriculture.Chemosensory system plays important roles in insect behaviors, some key genes governing chemosensation can potentially be used as novel targets for pest control. Nysius ericae (Hemiptera: Lygaeidae) is a polyphagous insect pest found throughout Eurasia countries which causes damage to many important crops. Little is known about the chemosensory mechanisms of host location and mate choice in N. ericae at themolecular level. Here, we assembled the antennal transcriptome of N. ericae using Illumina sequencing technology. Analysis of the transcriptome data identified 148 candidate chemosensory genes, including 28 for odorant binding proteins (OBPs), 16 for chemosensory proteins (CSPs), 2 for sensory neuron membrane proteins (SNMPs), 83 odorant receptors (ORs), 12 ionotropic receptors (IRs), and 7 gustatory receptors (GRs). Our results greatly improve the gene inventory for N. ericae and provide valuable resources for analyzing the functions of some key genes. This information will aid in the development of better understanding of chemosensation in N. ericae, and indicate novel targets for managing its impact on agriculture.

Insecticidal activities and biochemical properties of Pinellia ternata extracts against the beet armyworm Spodoptera exigua

Ya-Nan Zhang,Peng He,Jian-Ping Xue,Qing Guo,Xiu-Yun Zhu,Li-Ping Fang,Jin-Bu Li 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.2

The beet armyworm, Spodoptera exigua Hübner (Lepidoptera: Noctuidae), is a key pest of various agricultural crops in many countries throughout the world. The pest requires extensive use of pesticides and field-evolved resistances to conventional insecticides in China and other countries. Pinellia ternata Breit is native to the eastern part of Asia and foundmainly in China,which has been used in traditional Chinese medicines for N1000 years, but few studies have focused on the insecticidal activity of the P. ternate. In order to find natural products that could be used to control the pest in an safe, efficient and ecofriendlymanner,we first time analyzed the components of the anhydrous ethanolic extracts fromthe tubers of Pinellia ternate by using GC–MS method, and then investigated the insecticidal activities and biochemical mechanisms of the extracts against S. exigua. The result of GC–MS showed that 2-Methoxy-4-vinylphenolmay be an insecticidal active component, and we also found the extracts had notable insecticidal activity and disturbed the regular metabolismof S. exiguamainly through altering the activities of detoxification enzymes, digestive enzymes and protective enzymes. These properties suggest that the anhydrous ethanolic extracts from P. ternate can serve as a potential, efficient and ecofriendly S. exigua-control biopesticide.

Molecular identification and sex distribution of two chemosensory receptor families in Athetis lepigone by antennal transcriptome analysis

Ya-Nan Zhang,Ji-FangMa,Liang Sun,Zhi-PingDong,Zhao-Qun Li,Xiu-Yun Zhu,YiWang,LuWang,Dao-Gui Deng,Jin-Bu Li 한국응용곤충학회 2016 Journal of Asia-Pacific Entomology Vol.19 No.3

Chemosensory receptors play central roles in insect behavior and can potentially be used as new targets for pest control. Athetis lepigoneMöschler (Lepidoptera: Noctuidae) is a polyphagous insect pest found in many countries in Europe and Asia. However, systemic identification of the chemosensory receptors in the antennae has not been reported. In the present study, we obtained the antennal transcriptome of A. lepigone using Illumina sequencing technology and identified 80 candidate chemosensory receptors, including 61 transcripts encoding for odorant receptors (ORs) and 19 for ionotropic receptors (IRs). This result is similar to that reported for other lepidopteran species, suggesting that our approach successfully identified ORs and IRs of A. lepigone. RT-PCR was used to determine the sex distribution patterns of all OR and IR genes. Some OR and IR genes showed male- or femalespecific/ predominant expression. Our results help understand the functions of chemosensory receptor genes in A. lepigone as well as other insects, and suggest that these genes could be potential targets for developing environment-friendly behavioral antagonists and pesticides against A. lepigone.

Identification and expression patterns of UDP-glycosyltransferase (UGT) genes from insect pest Athetis lepigone (Lepidoptera: Noctuidae)

Ya-Nan Zhang,Ji-FangMa,Lu Xu,Zhi-PingDong,Ji-Wei Xu,Meng-Ya Li,Xiu-Yun Zhu 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.1

UDP-glucuronosyltransferase (UGT) genes,which belong to an ancient gene family and play very important roles in all organisms, encode extracellularly secreted proteins that are involved in the transfer of glycosyl residues fromactivated nucleotide sugars to acceptor hydrophobicmolecules (aglycones). Athetis lepigone is an important polyphagous pest worldwide, and since 2011 it has become one of the major maize pests in North China. However, there have been no studies on pesticides for the effective control of this pest. In this study,we identified 23 putative UGT genes in A. lepigone by analysing previous antennal transcriptomic data. Phylogenetic analysis showed that all of the AlUGTs are distributed within 11 of 14 insect UGT sub-families. Tissue expression analysis revealed that N70% of AlUGTs were primarily expressed in adult antennae, of which three (AlUGT33AD1, AlUGT40F6 and AlUGT40L4) and four (AlUGT33B18, AlUGT33F10, AlUGT40Q3 and AlUGT41D3) displayed male-biased and female-biased expression, respectively. SomeAlUGTs, however, had higher expression levels in non-antennal tissues. Our study is the first to identify UGT genes in A. lepigone,which will help us to elucidate the diverse functions of these genes, and ultimately provide potential targets that will facilitate the development of efficient and environmentally friendly pesticides against A. lepigone.

Molecular Cloning and Expression of a Novel Protease-resistant GH-36 α-Galactosidase from Rhizopus sp. F78 ACCC 30795

Ya Nan Cao,Ya Ru Wang,Hui Ying Luo,Peng Jun Shi,Kun Meng,Zhi Gang Zhou,Zhi Fang Zhang,Bin Yao 한국미생물 · 생명공학회 2009 Journal of microbiology and biotechnology Vol.19 No.11

Identification and tissue distribution of carboxylesterase (CXE) genes in Athetis lepigone (Lepidoptera: Noctuidae) by RNA-seq

Ya-Nan Zhang,Zhao-Qun Li,Xiu-Yun Zhu,Jia-Li Qian,Zhi-Ping Dong,Lu Xue,Peng He 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.4

Some of the metabolic enzyme carboxylesterases (CXEs) belong to the odorant-degrading enzymes (ODEs) family in insect species, and these play a key role in the degradation of acetate sex pheromones and host plant volatiles. Athetis lepigone is one of the most important agricultural insect pests in the world, can damage> 30 species of host plants, and has caused serious declines in the yield of summer corn in North China since 2011. According to our previous studies, the sex pheromone component of the pest is a binary blend of Z7–12:OAc and Z9–14:OAc at a ratio of 3:7. However, there are no reports regarding the degradation mechanism for these two sex pheromones. Herein, we firstly identified 20 candidate CXE genes in A. lepigone using our previous adult antennal RNA-seq data. Then, we constructed a phylogenetic tree and further conducted tissue distribution analyses to determine the possible functions of these genes. Our results showed that some AlepCXEs displayed adult antennae- predominant, male antennae-biased, or leg/wing-biased expression, indicating these AlepCXEs may have distinct physiological functions and play distinct roles in the degradation of sex pheromones, host plant volatiles, and/or other xenobiotics. These findings will help us to elucidate the exact functions of these genes in the future, and also provide possible target genes for the prevention and control of A. lepigone.

한국어 ‘됐어’와 중국어 ‘X了’의 대조 연구 -담화표지로서의 기능을 중심으로-

장아남 ( Ya Nan Zhang ) 국제한국어교육학회 2017 한국어 교육 Vol.28 No.4

The purpose of this study is to review the functions of {됐어} and {X了} as a discourse marker on different levels, and to examine their similarities and differences. {됐어} has not been widely recognized as a discourse marker in the field of Korean linguistics and Korean language education. Therefore, in order to establish the identity of {됐어} as a discourse marker, the reasons that {됐어} can be regarded as discourse marker were explained prior to the contrastive analysis. As to the method of contrastive analysis for {됐어} and {X了}, they were analyzed on three main dimensions: that is, the textual dimension, the interpersonal dimension, and the metalinguistic dimension in the corpus consisting of scripts of Korean and Chinese sitcoms. The results are as follows. In the textual domain, {됐어} and {X了} have the function of closing the topic in common, while {X了} can indicate a new topic and transmit a topic. In terms of functions in the interpersonal domain, {됐어} and {X了} are commonly used to refuse a partner's proposal or request and to interrupt a partner’s speech or action. Furthermore, in the interactional aspect, {됐어} and {X了} performs the function of expressing a response to a preceding utterance and taking the turn of speaking. The difference between them in the interpersonal domain is that {X了} performs the function of correcting a speaker’s utterance. In the metalinguistic domain, {됐어} and {X了} are common in that they perform the function of expressing the dissatisfaction of the speaker, showing generosity and making a compromise with the addressee. {X了}’s distinguishing characteristics in this domain is that it can express the attitude of consoling the hearer. (Korea University)

汉韩修饰“笑”的拟声拟态词的对比研究

장아남(Zhang, Ya-Nan)(张,雅楠),김현태(Kim, Hyun-Tae)(金炫兑,) 대한중국학회 2021 중국학 Vol.74 No.-

本文的研究目的是将汉韩修饰“笑”的拟声拟态词进行对比,并从其形态、语法、语义学、语用学等方面特征进行分析。本文通过对韩语词典《国语大词典(1992版)》和《标准国语词典》,还有汉语词典《象声词例释》、《现代汉语词典(第六版)》、《现代汉语大词典》、《现代汉语重叠形容词用法例释》的探究,整理出了韩语中修饰“笑”的拟声拟态词283个,汉语中修饰“笑”的拟声拟态词47个,并以此为研究资料进行了对比分析。有分析结果可以知道,韩语中修饰“笑”的拟声拟态词的数量比中文要多得多。而在形态方面,汉语和韩语修饰“笑”的拟声拟态词都有单独形和反复形两种形态,其中以反复形为主,反复形中又有整体反复形和部分反复形的形态。不同的是韩语中修饰“笑”的拟声拟态词较汉语来说,形态更为多样。语义方面,第一,韩语的拟声拟态词会随着形态的不同表现出不同的语感和含义,汉语中修饰“笑”的拟声词一般根据其不同的音韵特质可以感受出不同的语感差异。第二,汉语中修饰“笑”的双音节形态拟态词都是与动词“笑”结合,派生出具有新的含义的单词。而其含义也是由“笑”前面的字的含义所决定的。第三,汉语中修饰“笑”的三音节拟态词是“笑+BB”的形态,根据“BB”的音韵不同,其含义也会有细微的差异。通过对语义的分析可以知道,汉语的拟态词中基本都是和“笑”结合派生出的新词,但韩语中修饰“笑”的拟声词和拟态词是单独出现的。最后在语用方面,汉韩两种语言中修饰“笑”的拟声拟态词在使用时都有一定的限制,但明显汉语的限制没有韩语那么多。 The purpose of this paper is to compare and analyze the morphological characters of mimetic words modifying laughter in Chinese and Korean languages, and their characteristics in morphology, grammar, semantics and pragmatics. In the first chapter of this paper, the purpose and necessity of this subject are analyzed, and the corresponding research is carried out in advance. The second chapter is the definition and category advance of Chinese and Korean imitative words. This paper analyzes the morphological, grammatical, semantic and semantic features of the mimetic words modifying laughter in Chinese and Korean. By analyzing the above three chapters, this paper gives the following revelation: First, the modelling words of laughter in Korean are more abundant than in Chinese, so it is more difficult for Chinese learners to learn them. On the contrary, most of these words are derived from the combination of laughter, so we can generalize the meaning of the word and sum up the words containing laughter.Secondly, the mimic words of Korean are different in tone or form, and the meaning and meaning of Chinese language are different in tone or form, so both Chinese and Korean learners should master the morphology and tone characteristics of the modifier words in each other s language. Finally, both Chinese and Korean mimic words that embellish laughter have more or less restrictions on their use, so learners also need to grasp their linguistic characteristics.

한국어 학습자의 L2 학습 동기, 불안 및 자아 간의 상관관계 연구

장아남 ( Ya Nan Zhang ),김영주 ( Young Joo Kim ) 연세대학교 언어연구교육원 한국어학당 2014 외국어로서의 한국어교육 Vol.40 No.-

Teaching Korean as a Foreign Language, 40, 309~336. This study examined the effectiveness of the L2 motivational self system in the KSL education environment, and the roles of Korean learners’ L2 motivational self system between the foreign language anxiety and motivated behaviors. Survey was conducted with the foreign learners who are studying in the universities and the university-affiliated institutions. As for the results, the study found out that (i) the degree of formation of the ideal L2 self was higher than the ought-to L2 self,(ii) the ideal L2 self and the L2 learning experience in the L2 motivational self system showed positive correlation with the motivated behaviors except intermediate level learners, (iii) three aspects of the L2 motivational self system have correlation with the foreign language anxiety, (iv) clear negative correlation was found between foreign language anxiety and motivated behaviors except intermediate level learners. This study suggested that teachers in the KSL education environment need to provide classroom environment in which the ideal L2 self can be used actively.(Kyung Hee University)