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Succinyl Daidzin and Succinyl Genistin are New Isoflavone Derivatives Found in Cheonggukjang
SeungOk Yang,SeungWook Lee,YoungWoo Park,SangJun Lee,Pahn-Shick Chang,Sung-Seen Choi,JaeHwan Lee 한국식품과학회 2008 Food Science and Biotechnology Vol.17 No.1
Two new fermentation products were separated from cheonggukjang by high performance liquid chromatography (HPLC) and tentatively identified as succinyl daidzin and succinyl genistin by HPLC-mass spectrometry (MS). Both succinyl daidzin and succinyl genistin were detected in all 4 commercially available and one laboratory prepared cheonggukjang and 1 commercially available natto. However, these compounds were not detected in commercially available 4 home-made and 4 factory-produced doenjang. Peak areas of succinyl genistin were about 1.95-2.45 times higher than those of succinyl daidzin in cheonggukjang, which may be due to the higher concentration of genistin derivatives than daidzin derivatives in soybeans. This is the first report on the succinyl derivatives as isoflavone metabolites from cheonggukjang and these 2 isoflavone derivatives could be characteristic indicators for cheonggukjang.
Lee, Seungok,Park, Byung-Joon,Kim, Ji Yeon,Jekarl, DongWook,Choi, Hyun Yoo,Lee, Seong Yeoun,Kim, Myungshin,Kim, Yonggoo,Park, Moon-Seo Elsevier 2015 cytotherapy Vol.17 No.12
<P>Background aims. Perinatal tissues are considered an attractive source of mesenchymal stem/stromal cells (MSCs) and have unique characteristics depending on their origin. In this study, we compared the basic characteristics of unrestricted somatic stem cells isolated from cord blood (CB-USSCs) and MSCs isolated from Wharton's jelly of umbilical cords (WJ-MSCs). We also evaluated the effect of basic fibroblast growth factor (bFGF) supplementation on the growth and differentiation of these cells. Methods. CB-USSCs and WJ-MSCs were isolated from the same individual (n = 6), and their morphology, cell surface antigens, proliferation, expression of stemness markers and adipogenic, osteogenic and chondrogenic differentiation potentials were evaluated. Their morphology, proliferation and differentiation potentials were then also compared in the presence of bFGF supplementation (10 ng/mL). Results. Overall, CB-USSCs expressed DLK-1 and negative for all the HOX gene markers. The expression of cell surface antigen CD90, growth capacity and adipogenic differential potential of CB-USSCs were lower than those of WJ-MSCs. WJ-MSCs showed higher growth capacity, but the expression of CD73 and CD105 and their osteogenic differentiation potential were lower than those of CB-USSCs. The spindle morphology of both CB-USSCs and WJ-MSCs and the growth and adipogenic differentiation of CB-USSCs were improved by bFGF supplementation. However, the bFGF supplement did not have any positive effect on the tri-lineage differentiation potentials of WJ-MSCs. Conclusions. CB-USSCs and WJ-MSCs each had distinct characteristics including different growth capacity, distinguishable cell surface markers and distinct adipogenic and osteogenic potentials. bFGF supplementation improved the growth capacity and adipogenic differentiation of CB-USSCs.</P>
( Hae Mi Lee ),( Seungok Lee ),( Bon In Lee ),( Dong Wook Jekarl ),( Joo Yong Song ),( Hye Jung Choi ),( Bong Koo Kang ),( Eun Joo Im ),( Joon Sung Kim ),( Jong In Kim ),( Byung Wook Kim ),( Hwang Cho 대한소화기학회 2015 Gut and Liver Vol.9 No.5
Background/Aims: The diagnostic yield of fecal leukocyte and stool cultures is unsatisfactory in patients with acute diarrhea. This study was performed to evaluate the clinical significance of the fecal lactoferrin test and fecal multiplex polymerase chain reaction (PCR) in patients with acute diarrhea. Methods: Clinical parameters and laboratory findings, including fecal leukocytes, fecal lactoferrin, stool cultures and stool multiplex PCR for bacteria and viruses, were evaluated prospectively for patients who were hospitalized due to acute diarrhea. Results: A total of 54 patients were included (male, 23; median age, 42.5 years). Fecal leukocytes and fecal lactoferrin were positive in 33 (61.1%) and 14 (25.4%) patients, respectively. Among the 31 patients who were available for fecal pathogen evaluation, fecal multiplex PCR detected bacterial pathogens in 21 patients, whereas conventional stool cultures were positive in only one patient (67.7% vs 3.2%, p=0.000). Positive fecal lactoferrin was associated with presence of moderate to severe dehydration and detection of bacterial pathogens by multiplex PCR (21.4% vs 2.5%, p=0.049; 100% vs 56.5%, p=0.032, respectively). Conclusions: Fecal lactoferrin is a useful marker for more severe dehydration and bacterial etiology in patients with acute diarrhea. Fecal multiplex PCR can detect more causative organisms than conventional stool cultures in patients with acute diarrhea. (Gut Liver 2015;9:636-640)
A rapid antimicrobial susceptibility test based on single-cell morphological analysis
Choi, Jungil,Yoo, Jungheon,Lee, Mincheol,Kim, Eun-Geun,Lee, Ji Soo,Lee, Seungok,Joo, Seik,Song, Sang Hoon,Kim, Eui-Chong,Lee, Jung Chan,Kim, Hee Chan,Jung, Yong-Gyun,Kwon, Sunghoon American Association for the Advancement of Scienc 2014 Science Translational Medicine Vol.6 No.267
<P>A rapid antibiotic susceptibility test (AST) is desperately needed in clinical settings for fast and appropriate antibiotic administration. Traditional ASTs, which rely on cell culture, are not suitable for urgent cases of bacterial infection and antibiotic resistance owing to their relatively long test times. We describe a novel AST called single-cell morphological analysis (SCMA) that can determine antimicrobial susceptibility by automatically analyzing and categorizing morphological changes in single bacterial cells under various antimicrobial conditions. The SCMA was tested with four Clinical and Laboratory Standards Institute standard bacterial strains and 189 clinical samples, including extended-spectrum β-lactamase–positive <I>Escherichia coli and Klebsiella pneumoniae</I>, imipenem-resistant <I>Pseudomonas aeruginosa</I>, methicillin-resistant <I>Staphylococcus aureus</I>, and vancomycin-resistant <I>Enterococci</I> from hospitals. The results were compared with the gold standard broth microdilution test. The SCMA results were obtained in less than 4 hours, with 91.5% categorical agreement and 6.51% minor, 2.56% major, and 1.49% very major discrepancies. Thus, SCMA provides rapid and accurate antimicrobial susceptibility data that satisfy the recommended performance of the U.S. Food and Drug Administration.</P>
예비특수체육교사들의 교직 선택에 있어서 문화적응 시기의 영향
이홍민(Hongmin Lee),백승엽(Seung Yup Baek),안승옥(SEUNGOK AN) 서원대학교 사범대학 부설 교육연구소 2023 교육발전 Vol.43 No.1
본 연구는 예비특수체육교사들의 교직 선택에 있어서 교사사회화의 영향을 분석하는데 목적이 있다. 연구 대상자는 특수체육교육을 전공하는 대학생 3명과 대학원생 1명으로 구성되었다. 연구 참여자들을 대상으로 반구조화된(semi-structured) 질문지를 바탕으로 심층면담이 진행되었으며 문서자료를 수집하였다. 본 연구의 결과를 도출하기 위하여 귀납적 내용분석 방법을 활용하여 소주제로 부호화(coding) 과정 후 포괄적인 주제로 범주화하였다. 연구 자료로부터 네 가지 주제가 발견되었다. 첫째, 적극적인 신체활동은 특수체육교사로 직업을 선택하는 동기로 작용하였다. 둘째, 체육교사는 특수체육을 직업으로 선택하는데 영향을 미친 것으로 나타났다. 셋째, 친척과 가족의 신체활동에 대한 관심과 참여는 직업선택에 영향을 준 것으로 나타났다. 넷째, 자원봉사 활동 및 장애가족의 영향은 직업선택에 영향을 미친 것으로 확인되었다. The purpose of the current study was to analyze the impact of teacher socialization of preservice adapted physical education teachers on choosing adapted physical education as a career. The participants of the study consisted of one graduate and three undergraduate students who are majoring in adapted physical education. The data were obtained from in-depth interviews based on a semi-structured questionnaire and documents. This study used an inductive content analysis method based on coding sub-themes to comprehensive themes. Four key themes emerged from data: (1) active participation in physical activity motivated the participants' career choice to become an adapted physical education teacher, (2) the physical education teacher in physical education classes had an influence on choosing adapted physical education as a career, (3) it was found that the interests and active participation in physical activity and sports of relatives and family members influenced the participants' career choice, and (4) it was found that volunteer activities and family members with disabilities had influence on the participants' career choice.
Composotion and functional diversity of the vaginal microbiome in recurrent vaginitis
( Byeon Eun Jeong ),( Lee Seungok ),( Kim Myungshin ),( Kim Min Jeong ) 대한산부인과학회 2022 대한산부인과학회 학술대회 Vol.108 No.-
Objective: To evaluate the clinical relationship between the vaginal microbiome and the pathophysiology of recurrent vaginitis (RV), we investigated the microbiome taxonomic profile (MTP) in the vaginal samples of Korean female patients with RV. Methods: Forty women of reproductive age diagnosed with RV were enrolled. The vaginal MTP of patients was analyzed using 16S ribosomal RNA gene sequencing, and the results were compared with that of healthy women (n = 100). Further, the association of the vaginal community state type (CST) with the clinical characteristics was analyzed. Results: The species abundance of MTP was significantly lower in patients with RV than in healthy women (p < 0.05), whereas species evenness and diversity were significantly higher in patients with RV than in healthy individuals (p < 0.05). The proportion of the most common vaginal Lactobacillus spp. was significantly lower in the MTP of patients with RV than healthy women (p < 0.01). The beta diversity distance was also significantly different between patients with RV patients and healthy individuals (p = 0.001). Based on the CST, the MTP of 40 RV samples was categorized as follows: 21 (52.5%) for CST IV, 8 (20.0%) for CST III, 5 (12.5%) for CST I, 2 (5.0%) for CST II, 1 for (2.5%) for CST V, and 3 (7.5%) for mixed CST. Patients with underlying uterine diseases (uterine leiomyoma, adenomyosis, and endometrial polyps; n =17) showed higher species richness and diversity than those without (n = 23; p < 0.05). Conclusion: A low proportion of Lactobacillus spp. was found in patients with RV than in healthy women. The abundance and diversity of bacterial taxa were significantly higher in patients with underlying gynecologic disease than those without. Our study offers an insight into the nature of the vaginal microbiome and proposes that surveying the vaginal microbiome is valuable for detecting and treating gynecologic diseases in the future.
알루미늄 폼 및 허니컴 코어를 갖는 샌드위치 복합재료의 충격 특성에 관한 실험적 연구
최해규(Haekyu Choi),방승옥(Seungok Bang),이상교(Sangkyo Lee),조종두(Chongdu Cho),조재웅(Jaeung Cho) 한국자동차공학회 2011 한국자동차공학회 학술대회 및 전시회 Vol.2011 No.11
This paper studies the impact experiment about composite material with porous material. These specimens are the sandwich composites with cores of aluminum foam and honeycomb. The impact energies of 50, 70 and 100 J are applied at the specimen fixed at jig by Instron Dynatup. Maximum loading point or impact energy absorption at composite of foam core becomes higher than honeycomb core individually.