유도가열을 이용한 펌프수차 Shaft Nut Jack 분해

조용호(Yongho_Cho),김낙점(Nakjeom Kim),조성민(SeongMin Jo),조기현(Ki Hyun Cho),황미경(Mikyung Hwang),조민기(MinKi Cho),윤희철(HeeChul Youn),박선규(Sungyu Park),이태웅(TaeWoong Lee) 한국유체기계학회 2021 유체기계 연구개발 발표회 논문집 Vol.2021 No.12

Efficient Interleukin-21 Production by Optimization of Codon and Signal Peptide in Chinese Hamster Ovarian Cells

( Hee Jun Cho ),( Byung Moo Oh ),( Jong-tae Kim ),( Jeewon Lim ),( Sang Yoon Park ),( Yo Sep Hwang ),( Kyoung Eun Baek ),( Bo-yeon Kim ),( Inpyo Choi ),( Hee Gu Lee ) 한국미생물 · 생명공학회 2019 Journal of microbiology and biotechnology Vol.29 No.2

Interleukin-21 is a common γ-chain cytokine that controls the immune responses of B cells, T cells, and natural killer cells. Targeting IL-21 to strengthen the immune system is promising for the development of vaccines as well as anti-infection and anti-tumor therapies. However, the practical application of IL-21 is limited by the high production cost. In this study, we improved IL-21 production by codon optimization and selection of appropriate signal peptide in CHO-K1 cells. Codon-optimized or non-optimized human IL-21 was stably transfected into CHO-K1 cells. IL-21 expression was 10-fold higher for codon-optimized than non-optimized IL-21. We fused five different signal peptides to codon-optimized mature IL-21 and evaluated their effect on IL-21 production. The best result (a 3-fold increase) was obtained using a signal peptide derived from human azurocidin. Furthermore, codon-optimized IL-21 containing the azurocidin signal peptide promoted IFN-γ secretion and STAT3 phosphorylation in NK-92 cells similar to codon-optimized IL-21 containing original signal peptide. Collectively, these results indicate that codon optimization and azurocidin signal peptides provide an efficient approach for the high-level production of IL-21 as a biopharmaceutical.

RFLP법을 이용한 사상체질의 유전적 분석 연구

趙東郁,趙皇晟 현곡학회 1997 제3의학 Vol.2 No.1

In Sasang medicine, humen are classified into four constitutions which are Taevang, Sovang, Taeum and Soum. Depending on each different constitution, the clinical and pharmacological application for the same disease might be different. In this study, genomic DNA of different constitutions(Taeum, Soyang and Soum) were analyzed by Restriction Fragment Length Polymorphism(RFLP) to provide scientific and objective references for Sasang classification. The DNA polymorphism for each constitution detected as differences in the length of DNA fragments, after digestion with restriction enzyme Hae III, was investigated using YNH24 as DNA probe. The allele size of Taeum, Soyang and Soum group detected by YNH24 ranged from 1.3 to 3.8 kb, 1.5 to 3.9 kb and 1.3 to 4.6 kb, respectively. However, the allele size distribution of YNH24 loci of different constitutions was shown to be too variable to be classified as 3 different constitution groups investigated. For further study, it is suggested that the number of each constitution samples for RFLP analysis should be increased and statistical analysis of the allele size distribution should be carried out.

서비스수준을 고려한 GIS기반의 차량 운송시스템

황흥석,조규성 한국경영과학회 2001 經營 科學 Vol.18 No.2

This paper is concerned with the development of a GIS-based distribution system with service consideration. The proposed model could be used for a wide range of logistics applications in planning, engineering and operational purpose for logistics system. This research addresses the formulation of those complex problems of two-echelon logistics system to plan the incorporating supply center locations and distribution problems based on GIS. We propose an integrated logistics model for determining the optimal patterns of supply centers and inventory allocations (customers) with a three-step sequential approach. 1) First step, Developing GIS-distance model and stochastic set-covering program to determine Optimal pattern of supply center location. 2) Second step, Optimal sector-clustering to support customers. 3) Third step, Optimal vehicle route scheduling based on GIS, GIS-VRP. In this research we developed GUI-type program, the GIS_VRP provide the vehicle to users and freight information in real time. We applied a set of sample examples to this model and demonstrated sample results. It has been found that the proposed model is potentially efficient and useful in solving multi-depot problem through examples. However the proposed model can provide logistics decision makers to get the best supply schedule.

프레이트 포워더의 實態와 發展方向

黃正奉 제주대학교 1986 논문집 Vol.22 No.2

In der vorliegenden Arbeit habe ich versucht, 'Freight Forwarder' (Frachtspediteur) vorzustellen, der in den meisten europa¨ischen La¨ndern 'through transport'(Gesamtbefo¨rderung) zur Befo¨rderung der Iiandelsgilter u¨bernimmt, und emige Vorschla¨ge zu machen, damit die 'Forwarding industry'(Speditionsindustrie) sich als ein Befo¨rderungssystem in Korea niederlassen kann. Die Speditionsindustrie, die sich aus der Entwicklung des IIandelssystem ergibt, hat folgende Aufgaben Transport von Gutern, systematische Verbindung von den Transportmitteln, Konsolidation von den LCL-Gu¨tern, Verteilung, gegebenenfalls, Verpackung, Verzollung, Zahlung der Transportkosten, U¨berpru¨fung der Pracht usw. Nun will ich einige Vorschla¨ge nennen, damit der Frachtspediteur als ein Fachtransporter in Korea festgelegt wird. 1. 'Co-load system' soll aufgestellt werden, so daß sich 'through transport system' durchgesetzt wird. Der Kleinfrachter soll eine Gruppe bilden oder in der Verbindung mit dem Spediteur(einschl. NVOCC) an der Konsolidation der Gu¨ter teilnehmen. 2. Der Spediteur soll an seinen Aufgaben festhalten, und zwar an Ladung, Tranport, Lagerung, Zollabfertigung, Versicherung usw, wodurch der Frachter Ausgaben einschra¨nken kann. Wenn das Mißversta¨ndnis beseitigt wird, daß der Frachter doppelt ausgibt, la¨ßt sich erst die Speditionsindustrie niederlassen 3. 'Carfo data interchange system' u¨ber Handel und Transport soll sich noch weiter entwickeln. Das Nachrichtennetz u¨ber die Fracht im Hafen soll instalhert werden, um Daten u¨ber handel und Transport austauschen zu ko¨nnen. 4. Die Spezialita¨t, das Kapital, die Organisation und die Marktforschung vom Spediteur sollen eng mit dem Frachter zusammenha¨ngen. 5. Der Spediteur soll noch ein aktiveres Gescha¨ft treiben und eine bessere Anerkennung bei den frachtern finden. Die Regierung soll die Politik der internationalen Befo¨rderung verbessern, damit der Spediteur die Flugfracht transportieren und die Zollabfertigung u¨bernehmen kann. Dann kann der Spediteur im Handel eine wichtige Rolle spiclen als ein Informant, der dem Im-und Exporteur Bescheid u¨ber optimale Transportmittel und Route sagt, als ein Fachmann, der Verkaufs bedingungen auf der Basis von Verteilungskosten stellen kann, und der sich fu¨r das gu¨nstige Befo¨rderungssystem entscheidet, und als ein Aufseher, der die Gesamtbefo¨rderung kontrolliert. Die Im-und Exporteur ko¨nnen sich dann auch ausschließlich mit ihren eigentlichen Aufgaben d h Ein-und Ausfuhr bescha¨ftigen.

급성 호흡곤란으로 내원한 성대기능이상 1례

함초롬,박경일,박혜연,황철웅,김종연,김태년,진재용,이성순 白中央醫療院 2005 仁濟醫學 Vol.26 No.1

Vocal cord dysfunction (VCD) is a respiratory disorder characterized by the abnormal adduction of the vocal cords upon inspiration, expiration, or both, leading to a diverse array of acute obstructive airway signs and symptoms (wheezing, chest tightness, shortness of breath, and cough). VCD frequently mimics or confounds asthma. Misdiagnosis as asthma has led to inappropriate treatment, most notably with high-dose corticosteroids. Mistaken for anaphylaxis or angioedema, VCD has led to intubation and tracheostomy. VCD should be suspected in patients who are treated aggressively for asthma but continue to experience dyspnea. The diagnosis of VCD is made from direct visualization of the vocal cords during an attack. Inspiratory, anterior vocal cord closure with a posterior glottic chink is seen. On pulmonary function testing, a pattern of "saw-toothing", or fluttering of the inspiratory limb, representing fluctuations in the abnormal cord motion is seen in VCD patient. Treatment includes discussion of the diagnosis with the patients, discontinuing unnecessary medications, using of a helium-oxygen mixture in acute attack. Speech therapy and psychotherapy are the mainstays of long-term treatment. Here in we report a case of VCD in company with asthma.

일부 흡연 대학생의 지각된 스트레스와 흡연실태에 대한 상관관계 연구

강초록,권시내,권혜림,박수경,송인혜,오해인,이미지,한승아,황선아,김수지 이화여자대학교 간호과학대학 2003 이화간호학회지 Vol.- No.37

In spite of the seriousness of smoking harm has been developed these days. the increase of national smoking rate and that the people in the twenties are especially leading the rise of national smoking rate is an important issue of national health. Smoking of the university students can occur health problem and cause loss of manpower and expenses in their social activity period. So then the actual state for prevention of smoking and development of a program to prohibit smoking has to be seized urgently and starting with the study of the realities of university students, the effort to find a related fluent of smoking has to be studied. Related to this, the purpose of this study is to seize the general characteristics of smoking university students and to find out the relationship between perceived stress and smoking status among smoking university students. In addition, by collating the relationship between perceived stress and smoking status among smoking university students we have the purpose to furnish the basal data of stress care, the best way to cope with stress through group training and the plan for nursing implementation to decrease the smoking rate. For this study, a survey was conducted for 204 smoking university students of 13 universities in Seoul from 9th of May to 16th of May, 2003. The collected data was analyzed by t-test, Pearson Correlation and ANOVA in the statistical packages SPSS/PC. Major findings of this study are as follows; 1. Of the 204 respondents 85.4% of them were male and 14.6% of them were female. The average age of the sample was 23.19 years old and 41.8% of them were in senior, 21.4% in junior, 16.9% in sophomore, 19.9% in freshman grade. The major motivation to start smoking was 'because of curiosity'(33.8%) and the first smoking experience was at 'senior high school' (41.9%) and at 'college' (41.9%). 2 The highest scored stress factor of smoking university students were stresses about school work. 3. 72.7% had experience to quit smoking at least one or more times. Most of the reason to keep on smoking was 'short will to quit'(51.0%) and the smoking place was usually at 'school' (41.4%). Most respondents for the number ofsmoking friends were '5 people and above' (73.2%). 4. There was a negative relationship between the general characteristics and the entire perceived stress, however, there was a positive relationship between the general characteristics and the perceived stress in 4 categories which is 'sex distinction', 'major of study', 'residence form' and' inmate'. 5. There was a negative relationship between the general characteristics and the entire smoking status, however, there was a positive relationship between the general characteristics and the smoking status in 2 categories which is 'presence of hobby' and 'number of smoking friends'. 6. There was a negative relationship between the perceived stress and the smoking status. Through this study we have seized the relationship between the general characteristics, perceived stress and the smoking status of smoking university students. There was a positive relationship between some of the general characteristics with the perceived stress and the smoking status of smoking university students. Also there was a negative relationship between the perceived stress and the smoking status.

사상체질의학의 진화론적 고찰

지상은,조황성 한국한의학연구원 1997 한국한의학연구원논문집 Vol.3 No.1

Through the theoretical study on Sa-sang constitutional medicine from a evolutionary point of view, the result was obtained as follows. 1. The system of Sa-Sim-Sin-Mul(事心身物) in Sa-sang constitutional medicine is similar to the theory of evolution of Teilhard de Chardin. 2. The concept of Yin and Yang in Sa-sang constitutional medicine can be set up by the demand and necessity in the progress of evolution and the time of the differentiation of functions. 3. The Sung-Jung(性情) of each Sa-sang constitution can be explained as the strategy and form survival. 4. The theory of physiology, pathology and therapy in Sa-sang constitutional medicine can be hypothesized by the evolutionary standards which are related with the thermo-metabolism and hematopoietic-immune system.

장시간 불가피한 공복(fasting)후 인체의 내분비 변화 : 삼풍백화점 붕괴 사고후 구조된 3인의 증례 THREE CASES IN COLLAPSE OF SAMPOONG DEPARTMENT STORE

박규남,황주일,박조현,오동렬,이원재,오승택,김세경,김인철 대한응급의학회 1995 대한응급의학회지 Vol.6 No.2

The interactions between nutrition and the endocrine system are more intimate and complex that previously thought. Nutrition modulates secretion and activity of many hormones, as part of the stress adaptation process. Fasting, in particular, produces a number of important changes in the endocrine system, with teleological purpose of helping the body tolerate the lack of food ingestion. These changes are therefore beneficial and protective, although at times they may not appear to be that way. The endocrine changes of fasting mediate the metabolic response. We must emphasize that these changes are completely reversible when appropriate nutrition is established. These changes are as followes: 1) decreased insulin and increased glucagon levels, 2) decreased thyroid effect, 3) decreased sympathetic activity, 4) hypothalamic hypogonadism, 5) decreased growth, 6) altered glucocorticoid secretion and metabolism, 7) impaired mineralocorticoid response, 8) decreased ADH secretion and effect. We experienced three cases of involuntary prolonged fasting after collapse of Sampoong department store. They were released from collapsed field after 11(case Ⅰ), 13(case Ⅱ), 17days(case Ⅲ) respectively. In each case, the endocrine changes of plasma levels were as followes: decrease of cortisol in case Ⅰ, increase of GH in case Ⅱ, decrease of free T3, T3, cortisol and increase of reverse T3, insulin in case Ⅲ. Each one did not show the same endocrine changes of plasma levels. But we think these changes of fasting are beneficial in metabolic response of human.

우리나라 암등록 사업자료의 충실성에 관한 연구

이태용,조혜경,김광환 한국보건통계학회 2003 한국보건정보통계학회지 Vol.28 No.2

This research analyzed and compared centrally-required items and individual hospitals-developed items for cancer registration, as well as items for regional cancer registration projects. We then suggested additional item for standardization and improvement measures. We worked with a total of 161 medical agencies consisting of 118 general hospitals and 43 specialized general hospitals which take part in nation wide cancer registration statistics in Korea. A thesis submitted to the Committee of Graduate School of Public Health, Chungnam National University in partial fulfillment of the requirements for the degree of Master of Public Health conferred in August 2002. The following are the findings. First, the items of family disease history, smoking history, drinking history and favorite food surveyed only 0.6%-2.5% of the entire agencies, 2.3%-7.0% of the specialized general hospitals and 0.0%-0.8% of the general hospitals. Second, the items of TNM staging system, summary staging, and sites of metastasis investigated only 3.7%-5.0% of the total 161 agencies, 14.0% of the specialized general hospitals and 0.0%-1.7% of the general hospitals. Third, the items related to death and treatment investigated less than 2.5% of the entire hospitals, only 0.0%-7.0% of the specialized general hospitals and general hospitals not investigated. Finally, the items related to discharge and hospital utilization surveyed less than 4.3% of the entire hospitals, only 2.3%-11.6% of the specialized general hospitals and only 0.0%-1.7% of the general hospitals. Conclusively, the most hospitals missed important items. For that reason, investigation of valuable data insufficience. Therefore, improvement of nationwide cancer registration be caused by calculation of hospital, activated medical record library. The survey of cancer patients and medical treatment should be more efficiently and effectively conducted.