도토리 추출물의 3T3-L1 세포 분화억제 효과

김지연,이진,이창원,김애정,Kim, Ji-Yeon,Lee, Jin,Lee, Chang-Won,Kim, Ae-Jung 한국식품영양학회 2015 韓國食品營養學會誌 Vol.28 No.4

본 연구에서는 만성질환의 주요 위험인자인 비만을 예방하기 위한 식품소재로서 3T3-L1세포를 이용하여 탈피한 도토리의 항비만 효과를 알아보고자 하였다. 3T3-L1 세포에서 생존율(MTT assay)을 측정한 결과, AE와 AW 시료 모두 $500{\mu}g/mL$ 농도에서는 다소 생존율의 감소를 보여, $300{\mu}g/mL$를 최종 농도로 정하였다. 3T3-L1 세포의 지질축적 억제 효과를 측정한 결과, 농도 $100{\mu}g/mL$로 처리하였을 때 두 시료 모두 지질축적량의 증가를 보였으나, $200{\mu}g/mL$ 처리농도에서 AE 시료는 82%로, AW 시료는 74%로 감소되다가 $300 {\mu}g/mL$ 농도에서는 두 시료 모두 약 53% 수준까지 지질축적이 억제되었다. 3T3-L1 세포에서 중성지방 억제 효과를 확인한 결과, AE 시료의 경우 $200{\mu}g/mL$ 농도에서 11%의 감소율, $300{\mu}g/mL$ 농도에서 42% 수준의 감소율을 보였다. AW 시료도 $200{\mu}g/mL$ 농도에서 5%의 감소율과 $300{\mu}g/mL$에서 41%의 감소율을 보였다. 3T3-L1 세포의 ROS 생성량을 측정한 결과, 시료 농도 $200{\mu}g/mL$에서 AE는 42%, AW는 33%로 $300{\mu}g/mL$에서는 AE는 58%, AW는 52%로 ROS 생성량의 억제를 보였다. 3T3-L1 세포에서 mRNA 발현에 미치는 영향을 대조군과 비교하였을 때 두 시료(AE와 AW) 모두 $300{\mu}g/mL$ 농도에서 $PPAR-{\gamma}$은 54%와 38%, aP2는 40%와 18% 수준의 발현을 억제시키는 것으로 나타났다. 이상의 결과로 볼 때 탈피한 도토리는 3T3-L1 세포의 분화를 억제함으로써 새로운 항비만 소재로의 가능성이 있는 것으로 판단된다. This study aimed to investigate the suppressive effect of acorn extracts, by evaluating 70% ethanol extract (AE) and hot water extract (AW) using 3T3-L1 preadipocytes. We applied various levels (0, 100, 200, 300 and $500 {\mu}g/mL$) of AE and AW to 3T3-L1 preadipocytes. The cell viability of the 3T3-L1 preadipocytes was not affected by up $300 {\mu}g/mL$ of extracts, but was suppressed by level $500{\mu}g/mL$ of both AE and AW by 20% and 9% respectively. The accumulation of lipid droplets in differentiated 3T3-L1 preadipocytes was dose-dependently suppressed by AE and AW. Especially, at high concentrations ($300{\mu}g/mL$), AE (42%) was more effective than AW (41%). Reactive oxygen species (ROS) was also dose-dependently suppressed by treatment with AE (58%) and AW (52%). With regard to the mRNA related to differentiated 3T3-L1 preadipocytes, $PPAR-{\gamma}$ and aP2 were suppressed by treatment with AE (54 and 40%) and AW (38 and 18%). From our results, acorn extract (AE) has more suppressive effects than AW in differentiated 3T3-L1 preadipocytes. We therefore concluded that acorn has suppressive effects against obesity in differentiated 3T3-L1 cells due to antioxidation.

간호 학생의 임상실습 효율을 위한 기초 조사 연구

이애경,김정애,주미경,정안순,배경진,김정수,이정애,장은정 경복대학 2000 京福論叢 Vol.4 No.-

임상실습 교육은 간호이론을 실무에 적용하는 기회를 제공하는 과정으로서 임상실습이 간호교육의 중요한비중을 차지하고 있다는 것은 주지의 사실이다. 이러한 중요성에도 불구하고, 임상실습 교육에 즉각적인 해결이 어려운 많은 문제점이 있음도 이미 알고 있는 사실이다 이에 임상실습 교육의 기초 자료로 활용되고 있는 실습목록표에 대한 간호 학생들의 실습목표의 명확한 이해와 더불어 이론과 실습의 연계성을 강화시키고자 하였다. 성인간호, 모성간호, 이동간호 3개 영역 모두에서 공통적으로 기본임상간호 항목이나 기본간호술과 관련된 항목에서 직접수행의 빈도가 높았고 숙련된 기술적인 처치나 전문성을 필요로 하는 항목에서는 관찰과 수행경험이 부족한 것으로 나타났다. Clinical performance is not only the process of providing the opportunity for applying nursing theory into practice but also it is a well-known fact that clinical performance is an important part in nursing education. lnspite of this importance, it is also true that many problem with no immediate solution exist in clinical performance of nursing students. thus, through measuring and analyzing the degree of clinical experience of the nursing students, on the nursing checklist that is being used as the basic guide in clinical education, we tried to clearly understand the objectives of clinical performance and to emphasize the connection between theory and clinical performance. The results of the study showed that the frequency of directly performing tasks was high in all areas of adult health nursing, maternity nursing, and child nursing for the items of basics clinical nursing and items related with basic nursing techniques, and that the experiences of observation and execution were lacking in the items needing skilled treatment or speciality.

해조류를 이용한 항생재 대체 사료 첨가제 개발

박진용,김진욱,이희현,이송애,김진욱,이재화 신라대학교 자연과학연구소 2005 自然科學論文集 Vol.14 No.-

우리나라의 항생제 남용 문제는 세계적으로 심각 하다고 한다. 이러한 문제를 해결하기 위해서 정부는 다양한 정책을 도입하고 있으며 특히 축산물의 항생제 남용 문제를 해결하기위해 사료 관리법을 강화하여 항생제 사용을 억제하고 있어 항생제를 대체 할 물질이 필요하다. 이런 문제를 해결하기 위해서 우리나라의 다양한 해조류를 이용한 항생제 개발을 통해서 항생제 남용 문제를 해결하고 소비자들에게 믿을 수 있는 축산물을 제공 할 것으로 보인다. Feed official law was strengthened recently. The antibiotic use is suppressed with feed official law reinforcement. The antibiotic transfer material is necessary. To use the seaweeds and transfer the antibiotic. It is a possibility of a high income. This is new bio industry. This the goods production which is competitive power is possible. It is a possibility of eating the livestock where also the consumers are good.

한국산 약용식물의 화장품천연소재로서 응용에 관한연구

안봉전,이진태,이순애,곽재훈,박정미,이진영,박태순,손준호 경산대학교 생명자원개발연구소 2003 생명자원과 산업 Vol.7 No.-

Biological activities and application of sanguisorbae officinalis L. were investigated. In the enzymological physiological activities, the electron donating ability(EDA) was 54.92% in 10 ppm and it was over 90% over 50ppm and SOD-like activity was high as 65.36% in 1000 ppm, it was gradual increased. As inhibitory effect of xanthine oxidase, it was 17.90% in 200 ppm and a little low as 36.89% in 500 ppm and inhibitory effect of tyrosinase, it was a little low as 20.45% below 1000 ppm. As the result of measuring the lipid oxidation, all the concentrations of medical ion treatments had the ability to keep it from acidification and metal ion blocking effects about the lipid oxidation promoting factors(Fe^(2+) and Cu^(2+)), Fe^(2+) was better than Cu^(2+) and all concentrations of medical ion treatments was 40% in 50ppm. When it was applied into normal skin-softener it showed safe effect so that we can expect that as the natural material of cosmetics.

숙명여자대학교 문신미술관 리브랜딩 프로젝트 -마케팅전략을 통한 디자인 문제점 해결을 중심으로-

류혜나,박예진,손예빈,송혜진,이종화,이진민,장미정,이성애 숙명여자대학교 산업디자인연구소 2017 숙명디자인학 연구 Vol.24 No.-

본 연구는 한국의 뿌리, 동양의 미의식을 동시에 지닌 숙명문화원의 가치를 끌어올리기 위해 ‘브랜드, 디자인, 마케팅’을 융합된 시점에서 바라보고 그래픽, 제품, 공간의 통합 및 구축하는 일련의 프로세스를 밝히는데 목적이 있다. 이에 본 연구 시간적 범위는 2017년 3월~6월까지 조사대상은 브랜딩이 미흡한 숙 명문화원의 문신미술관, 숙명박물관, 숙명역사박물관, 정영양자수박물관으로 한정한다. 그 결과, 디자인과 마케팅의 융합된 측면에서 도출된 브랜드스토리인 ‘문신의 작은 우주’는 조각가 문신의 가치관과 정체성을 유지하면서 지역사회와 소통하는 미술관으로서 포지셔닝 되었다. 또한, 문신미술관은 디자인과 마케팅 측면을 융합하여 로고, 사인물, 에코백, 머그컵 및 전시기획 등에 이르기까지 일관성과 객관성, 스토리성을 띄었다. 끝으로 본 연구는 문신미술관의 디자인과 마케팅을 융합하여 좀 더 체계화된 G·I, P·I, S·I의 통합 브랜드 매뉴얼을 만든 것에 의의가 있다

중합효소연쇄반응을 이용한 도말양성 폐결핵 환자에서의 결핵균 DNA 검출방법에 관한 연구

이종진,김애경,조해정,한표성,홍석철,김주옥,김선영 충남대학교 의과대학 지역사회의학연구소 1994 충남의대잡지 Vol.21 No.2

Polymerase chain reaction (PCR) is the most sensitive and rapid method for the diagnosis of M.tuberculosis infection. Many reports have shown different methods for mycobacterial DNA extraction, but revealed many differences in simplicity and time-consumption. We studied which method was the best among 7 different ones for DNA extraction from 32 smear-positive sputa, using PCR of targeting 123bp DNA fragment of IS6110. The methods were the following : SDS-Microwave oven method, NaOH Lysis method, Triton X-100, Proteinase K method, Lysis buffer method. SDS-Proteinase K method, Bead Beater method, and Bead Beater/CTAB method. The amplification products were identified by electrophoresis on ethidium bromide-stained agarose gels, and confirmed by restriction enzyme, Southern blot hybridization, and DNA sequencing. All 32 cases were positive (100%) by the 7 different methods. It took 1 hour or more to detect DNA in SDS-Microwave oven method, NaOH Lysis method, and Bead beater/CTAB method. It took 2 hours or less in the others. SDS-Proteinase K method and Bead Beater/CTAB method were simpler and more convinient than the others. These results suggest that SDS-Proteinase K method and Bead Beater/CTAB method might be better than the others for the extraction of mycobacterial DNA in terms of simplicity and time-consumption, although all the methods were sensitive.

탈지대두박을 활용한 콩치즈와 스폰지 케익의 품질특성

이숙영,박미정,최애진 중앙대학교 생활과학연구소 2001 생활과학논집 Vol.14 No.-

The effects of the different mixing ratios(10:0, 7:3, or 5:5) of Jinpum soybean milk to defatted soybean meal milk and the different enzyme treatments with α -chymotrypsin or trypsin on the yields, physicochemical and textural properties, and sensory characteristics of soybean cheeses were studied. The effects of the different replacement amounts(15, 30, or 45%) of defatted soybean meal on the physicochemical and sensory characteristics of sponge cake were also studied. In the results of yield, pH, lightness, hardness, mouthfeel and overall quality of sensory characteristics, the mixing ratio of 7:3 and the trypsin treatment were recommended for better manufacturing of high-quality soybean cheese. In the results of moisture contents, specific gravity, and overall quality of sensory characteristics, there were no significant differences between control and the sample of 15% replacement with untreated defatted soybean meal, and also between control and that of 30% replacement with trypsin-treated. The trypsin treatment of defatted soybean meal resulted in similar texture to control, the decrease in beany flavor, softer mouthfeel, and better moistness.

마늘의 효능과 안전성에 관한 연구

이애정;이상화;조강진;김인혜;엄애선 한양대학교 2010 韓國 生活 科學 硏究 Vol.30 No.1

Galic is the fresh or dehydrated bulb obtained from Allium scorodorpasum var. viviparum Regel, a genus of the lily family. Garlic has been widely used as oriental medicine having various health benefits, such as cardiovascular, antineoplastic, antimicrobial and antiinflammatory. The average daily intake in western people is 4g/day of fresh garlic whereas it in Koreans 5.8g/day. However, consumption of excessive amount of raw garlic can cause gastrointestinal disease, dermatitis, asthma, allergic reaction, anticoagulant action, breath and body odor. Additionally, its antithrombotic properties prompt patients to stop taking garlic 14 days before having surgery or dental work. Therefore, we reviewed the safety of garlic on the basis of literatures published and then further studies on the safe amount of garlic consumption should be considered.

일개 종합병원중심 가정간호 간호진단분류를 위한 NANDA와 HHCC의 적용 비교

이진경,박현애 성인간호학회 2000 성인간호학회지 Vol.12 No.4

Application of NANDA and HHCC to Classification of Nursing Diagnosis in a Hospital-Based Home Health Care This study examines that Noith American Nursing Diagnosis Association(NANDA) and Home Health Care Classification(HHCC) is appropriate to classify home health care client's nursing problems and suggests a modified nursing diagnosis classifcation system. Two hundred and forty-nine clients' records at a general hospital were reviewed and nursing problems were diagnosed according to each classification system. Results of this study are as follows. The major client's medical diagnosis are pregnancy, childbirth and puerperium. malignant neoplasm, and benign neoplasm. Of four hundred and sixty-three nursing problems, all nursing problems made a diagnos according to HHCC, while three hundred and eighty-five made a diagnosis according to NANDA. The HHCC diagnosis included 78 more nursing problems than NANDA The discrepancy in the results may indicate a significant advantage to HHCC diagnosis because HHCC nomenclature was created empirically from hard data. However, this may be due to limitations in the data collection method so determination of which classification system is more useful is difficult to judge. However, nursing components of the HHCC are more concrete and clearer than human response patterns of the NANDA. Also the HHCC facilitates the documentation of Patient care by computer, while using a conceptual framework consisting of 20 Care Components based on the nursing process: assessment, diagnosis, outcome identification, planning, implementation and evaluation. Accordingly, the practical application of HHCC is more useful than NANDA. Limitations of this study include a retrospective data collecting method and universality of samples. Further research for various samples that use prospective data collection method is recommended.

정신분열병의 유전적 고위험군에서 인지, 정서 및 사회기능 연구

이경진,위휘,유소영,이애라,송지연,하태현,홍경수,김명선,권준수 大韓神經精神醫學會 2006 신경정신의학 Vol.45 No.1

Objectives : Vulnerability marker in biological relatives of schizophrenia may be very useful in identifying the propensity for the development of schizophrenia. This study suggests that the relatives at risk for genetically developing schizophrenia have impairments in several domains of cognition, social function and affective function as a vulnerability marker. Methods : A neurocognitive function test, a social function test and an affective function test were administered to fifteen healthy relatives from families with two or more patients with schizophrenia (geneticallly high risk subjects). Fifteen healthy controls matched for age and gender were recruited in this study. Results : Compared to the control subject, the genetic high risk subjects performed significantly more poorly in the selective attention and recall memory of neurocognitive function. The high dsk subjects also had lower scores in independence-performance and independence-competence of the social function test and emotion control of the affective function test. Conclusion : Selective attention, recall memory, independence-performance, independence-competence and emotion control ability may be a valuable marker for genetic study of schizophrenia.