Protective Effects of N-acetylcystenine and Selenium against Doxorubicin Toxicity in Rats

Yeo-sung Yoon,Sun-don Kim,Min-hye Lee,Heung-shik S.Lee,In-se Lee,Je-kyung Sung,Eun-sung Park 대한수의학회 2003 Journal of Veterinary Science Vol.4 No.2

Effects of N-acetylcysteine and Selenium against Doxorubicin Toxicity in RatsEun-sung Park, Sun-don Kim1, Min-hye Lee, Heung-shik S. Lee, In-se Lee, Je-kyung Sung and Yeo-sung Yoon*

초기 자궁내막암 환자에서의 복강경유도하 질식 자궁절제술과 복식 자궁절제술에 대한 비교 연구

김민규 ( Min Kyu Kim ),최동석 ( Dong Seok Choi ),김우영 ( Woo Young Kim ),최철훈 ( Chel Hun Choi ),김태중 ( Tae Joong Kim ),이정원 ( Jeong Won Lee ),김병기 ( Byoung Gie Kim ),이제호 ( Je Ho Lee ),배덕수 ( Duk Soo Bae ) 대한산부인과학회 2006 Obstetrics & Gynecology Science Vol.49 No.12

목적: 초기 자궁내막암의 수술적치료 시 복강경유도하 질식 자궁절제술 및 골반림프절절제술군과 복식수술군의 수술결과 및 재발율에 대해 비교하고자하였다. 연구 방법: 2003년 3월부터 2005년 5월까지 초기 자궁내막암 환자를 인구학적 특성이 같은 63명을 선택하여 복강경유도하 질식 자궁절제술 및 골반림프절절제술군 26명, 복식수술군 37명으로 나누어 수술 전후 혈색소 차이, 평균수술시간, 취득한 골반림프절갯수, 평균재원일수, 추가치료율, 재발률을 비교하였다. 결과: 복강경유도하 질식 자궁절제술 및 골반림프절절제술을 시행한 군과 복식수술을 시행한 군과의 비교에서 수술 전후 혈색소 차이, 평균수술시간, 취득한 골반림프절 갯수, 평균재원일수, 재발률에 통계적으로 유의한 차이가 없었다. 결론: 초기 자궁내막암의 수술 시 복강경유도하 질식자궁절제술 및 골반림프절절제술 방법이 복식방법에 비해 수술결과 및 재발률에 통계적으로 유의한 차이가 없으며 그 대체 수술법으로 많은 활용이 기대되었다. Objective: To compare laparoscopic surgery with conventional abdominal surgery in patients with early stage endometrial cancer. Methods: A retrospective review of 63 patients with early stage endometrial cancer managed between March 2003 and May 2005. Two groups were defined whether they had been treated by laparoscopy (case group: n=26) or by laparotomy (control group: n=37). We compared age, body mass index (BMI), hemoglobin change, operation time, number of pelvic lymph nodes, hospital stay, case with adjuvant treatment and recurrence between two groups. Results: There was no statistical difference in characteristics (age, BMI, nulliparity, previous abdominal surgery, FIGO stage, histologic grade). between case and control group. In addition, there was no statistical difference in operation data and outcomes between two groups. Hemoglobin changes were 1.1 g/dL (case group) vs 1.7 g/dL (control group) (p=0.072). Operation time was 131 min vs. 115 min. The numbers of lymph nodes obtained were 8.7 vs 7.7 (right) 9.2 vs. 7.6 (left). Hospital stays were 8.4 vs. 9.2 days. Adjuvant treatment cases were 7 vs. 15. Recurrent case was one in each group. Two patients initially evaluated by laparoscopy were converted to laparotomy due to bleeding and adhesion. Conclusion: Laparoscopic surgery for treatment of early endometrial cancer is a safe and effective alternative to laparotomy . However, long-term survival and risk of recurrence have yet to be determined.

High-performance Liquid Chromatographic Analysis for Quantitation of Marker Compounds of Artemisia capillaris Thunb

( Kyung Min Park ),( Ying Li ),( Bora Kim ),( Haiyan Zhang ),( Kyong Hwangbo ),( Dong Gen Piao ),( Mei Juan Chi ),( Mi Hee Woo ),( Jae Sue Choi ),( Je Hyun Lee ),( Dong Cheul Moon ),( Hyeun Wook Chang 영남대학교 약품개발연구소 2013 영남대학교 약품개발연구소 연구업적집 Vol.23 No.0

Two stable high-performance liquid chromatography (HPLC) methods were developed that could quantitatively analyze 10 major marker compounds of Artemisia capillaris Thunb and could also distinguish among `Injinho` and `Myeon-injin` and `Haninjin`--A. capillaris collected in autumn, A. capillaris collected in spring and A. iwayomogi, which can be misused as `Injinho` in Korean herbal drug markets. The first HPLC method was a reversed-phase chromatography using a C18 column with an isocratic solvent system of phosphoric acid (0.05%) and acetonitrile at the flow rate of 1.0 mL/min, ultraviolet (UV) detection wavelength at 254 nm and column temperature at 40°C. Calibration andquantitation were made by using acetaminophen as an internal standard (I.S-A) and chlorogenic acid (1) was determined within 20 min. The second HPLC method was a reversed-phase chromatography using a C18 column with a gradient solvent system of phosphate buffer (0.015 M, pH 6) and acetonitrile at the flow rate of 1.0 mL/min, UV detection wavelength at 254 nm and column temperature at 40°C. Calibration and quantitation were made by using ethylparaben as an internal standard (I.S-B) and 3,5-di-O-caffeoylquinic acid (2), 3,4-di-O-caffeoylquinic acid (3), 4,5-di-O-caffeoylquinic acid (4), hyperoside (5), isoquercitrin (6), isorhamnetin 3-O-robinobioside (7), isorhamnetin-3-O-galactoside (8), isorhamnetin-3-O-glucoside (9) and scoparone (10) were determined within 60 min. Pattern recognitionanalysis of data from the 60 samples classified them clearly into three groups. These assay methods could be applied for QA/QC of A. capillaris and Artemisia iwayomogi.

다양한 적송잎 추출물의 항생제 내성 황색포도상구균에 대한 항균활성 검증

Nam-Young Kim(김남영),Min-Kyung Jang(장민경),Myung Je Jeon(전명제),Dong-Geun Lee(이동근),HyeJi Jang(장혜지),Seung Woo Lee(이승우),Mihyang Kim(김미향),Sung Gu Kim(김성구),Sang-Hyeon Lee(이상현) 한국생명과학회 2010 생명과학회지 Vol.20 No.4

병원 내 감염의 주된 원인균으로 알려진 황색포도상구균(5. aureus)과 항생제 내성 황색포도상구균(PRSA, MRSA)에 대해 항균활성이 우수한 천연 항균성 물질을 검색하기 위해 적송잎을 대상으로 열수-헥산(HWH), 열수-에탄올(HWE), 헥산, 에탄올 및 열수 등으로 획득된 추출물들로 항균활성을 조사하였다. 헥산, 에탄올, 열수-헥산, 열수-에탄올 추출물들은 그람양성 대표세균인 고초균(B. subfilis), 황색포도상구균 표준균주, 항생제 내성균주인 PRSA 및 MRSA에 대해서 항균활성을 나타내었으며, 특히 MRSA (ATCC 33591) 균주에 대해 다른 균주들 보다 높은 항균활성을 나타냈다. 열수 추출물은 황색포도상구균 표준균주 및 MRSA에서만 항균활성을 나타냈지만, 항균활성은 가장 높아 50 ㎎/㎖의 농도에서 33 ㎜의 생육저해환을 나타냈다. 각 균주들에 대한 생장의 최소저해 농도(MIC)는 HWH 및 HWE 추출물이 모두 0.05 ㎎/㎖의 농도로 가장 낮았고, 열수추출물이 0.5 ㎎/㎖의 농도였으며, 에탄올 추출물은 5 ㎎/㎖의 가장 높은 농도를 나타냈다. 모든 추출물들은 121℃에서 20분간의 열처리 후에도 항균활성이 유지되었으므로 열에 대해 안정한 물질임을 알 수 있었다. 본 연구결과로 식품산업에서 솔잎 추출물을 천연 항균제로 사용할 수 있을 것으로 기대할 수 있다. investigated antimicrobial activities of various pine (Pinus densiflora) needle extracts against antibiotic resistant strains of Staphylococcus aureus. Hot water extract showed the highest antimicrobial activity against normal and methicillin resistant Staphylococcus aureus (MRSA), however, it exhibited no antimicrobial activity against penicillin resistant S. aureus (PRSA). Hot water-hexane (HWH), hot water-ethanol (HWE), hexane, and ethanol extracts showed antimicrobial activity against S. aureus, PRSA and MRSA. Minimum inhibitory concentrations (MIC) of HWH, HWE, hexane, and ethanol extracts were 0.05, 0.05, 0.5 and 5 ㎎/㎖ respectively, and HWH and HWE extracts showed the strongest antimicrobial activity among these extracts. Antimicrobial activities of pine needle extracts were stable after heating at 121℃ for 20 min. These results suggested that pine needle extracts can be used as an effective natural antimicrobial agent for food and medical industries.

태권도 돌려차기 시 기술 수준에 따른 몸통, 골반, 지지 다리의 운동학적 변인 비교

김재웅 ( Kim Jae-woong ),한기훈 ( Han Ki-hoon ),김제민 ( Kim Je-min ),이상우 ( Lee Sang-woo ),권문석 ( Kwon Moon-seok ),터틀노엘 ( Noelle J. Tuttle ) 국기원 2018 국기원태권도연구 Vol.9 No.1

이 연구의 목적은 태권도 돌려차기 시 기술 수준에 따른 몸통, 골반, 지지 다리의 운동학적 변인을 비교 분석하는 데 있다. 본 연구를 위해 블랙 벨트 소지자로서 기술 수준에 따라 중급자10명, 상급자 9명이 실험에 참여하였다. 3차원 동작 분석 시스템을 사용하여 골반, 몸통, 지지다리의 방향각을 산출하였다. Start, Toeoff, MKF, Max, Impact, Min에서 그룹 간의 평균 방향각을 비교하기 위하여 독립표본 t-검정을 하였다. MKF, Impact, Min에서 골반의 좌측 기울어짐 각도가 그룹 간의 유의한 차이가 나타났다. Start와 Max에서 지지 다리의 엉덩관절 굴곡 각도와 MKF에서 지지 다리의 엉덩관절 외전 각도 사이에 유의한 차이가 있었다. 이상의 결과를 볼 때, 상급자들은 푸쉬단계에서 엉덩관절의 굴곡 각도가 증가 되었고, MKF에서는 외전 각도가 감소하였다. 또한, 스트라이크 단계에서 골반의 좌측 기울어짐 각도는 감소하였다. The study purpose was to investigate skill level differences on trunk, pelvis, and supporting leg kinematics in a Taekwondo roundhouse kick. 10 intermediate and nine expert male black-belt holders executed roundhouse kicks at a target. Orientation angles of the pelvis, trunk, and supporting -thigh, -shank, and -foot were obtained through a three-dimensional video motion analysis. Orientation angles at Start, Toeoff, Maximum Knee Flexion(MKF), Impact, Maximum angle(Max), and Minimum angle(Min) were compared between groups using independent t-tests(p<.05). Significant differences between groups were found in the pelvis left lateral tilted position at MKF, Impact, and Min; significant differences were also found in the supporting hip joint flexed position at Start and Max, and abducted position at MKF. It was concluded that higher performers increased the hip joint flexion angle in the Push phase, decreased abduction angle at MKF, and decreased the pelvis left tilted angle in the Strike phase.

Purification and Characterization of Helicobacter pylori ${\gamma}$-Glutamyltranspeptidase

Song, Jae-Young,Choi, Yeo-Jeong,Kim, Jeong-Min,Kim, Yoo-Ree,Jo, Jin-Seong,Park, Jin-Sik,Park, Hee-Jin,Song, Yun-Gyu,Lee, Kon-Ho,Kang, Hyung-Lyun,Baik, Seung-Chul,Youn, Hee-Shang,Cho, Myung-Je,Rhee, Kw The Korean Society for Microbiology 2011 Journal of Bacteriology and Virology Vol.41 No.4

Gamma-glutamyltranspeptidase (GGT) was purified to electrophoretic homogeneity from the cell extract of H. pylori. The purified enzyme consisted of heavy and light subunits with molecular weights of 38 kDa and 21 kDa, respectively. N-terminal amino acid sequence of heavy and light subunits revealed that H. pylori GGT was processed into 3 parts for a signal peptide of 27 amino acid residues, a heavy subunit of 352 residues, and a light subunit of 188 residues during translation. The reaction rate for hydrolysis of ${\gamma}$-GpNA was 84.4 ${\mu}mol/min$ per milligram of protein, and that for the ${\gamma}$-glutamyl transfer from ${\gamma}$-GpNA to gly-gly was 23.8 ${\mu}mol/min$ per milligram of protein. The apparent Km values of H. pylori GGT for ${\gamma}$-glutamyl compounds were on the order of $10^{-3}$ to $10^{-4}$ M and those for acceptor peptides and amino acids were on the order of $10^{-1}$ to $10^{-2}$ M. The GGT protein kept approximately 80% of the initial enzymatic activity on incubation at $60^{\circ}C$ for 15 min. The optimum temperature and pH for reactions of both hydrolysis and transpeptidation were $40^{\circ}C$ and 9.0, respectively. The transpeptidation and hydrolysis reactions catalyzed by H. pylori GGT were strongly inhibited by L-Gln and moderately inhibited by L-Ala, L-Ser, ${\beta}$-chloro-L-Ala, and L-Glu. These results demonstrated that the biochemical properties of H. pylori GGT are different from those of other bacterial GGTs. Further, H. pylori GGT might degrade glutathione in the gastric mucous layer of humans if the enzyme could be secreted in the bacterial niches.

중증 재생불량성 빈혈 환자의 동종골수이식 후에 병발한 간정맥폐색질환(Hepatic Veno-Occlusive Disease)의 임상 경과 및 혈액응고 인자들의 변화

이제환,이규형,최성준,민영주,김신,이정신,김상희,박찬정,지현숙,김우건 대한조혈모세포이식학회 1999 대한조혈모세포이식학회지 Vol.4 No.2

연구배경: 재생불량성 빈혈 경우에는 조혈모세포 이식 후 VOD의 발생에 관한 보고가 거의 없는 실정이다. 저자 등은 cyclophosphamide와 antithymocyte globulin으로 전처치 후에 동종골수이식을 시행한 재생불량성 빈혈 환자들을 대상으로 하여 VOD의 발생과 임상 양상, 혈액응고 인자들의 변화에 관하여 분석하여 보았다. 방법: 재생불량성 빈혈로 동종골수이식을 시행 받은 13예 환자를 대상으로 하였다. 임상적 기준에 따라 VOD를 진단하였으며 임상 경과에 따라서 경중을 분류하였다. Protein C, protein S, antithrombin Ⅲ, tPA, PAI-1 등의 혈액응고 인자들을 전처치 투여전, 골수 주입당일, 이식 후 제7, 14, 21일에 측정하였다. 결과: 7예(53.8%)에서 VOD가 발생하였으며, 경증 5예, 중등도 2예였다. VOD군에서 혈소판치가 20.000/㎕ 이상을 유지하는 데까지 걸리는 시간이 더 길었다(p=0.026). 또한 VOD군에서 적혈구 및 혈소판의 수혈 요구량이 각각 유의하게 많았다(p=0.025, 0.015). 혈액응고 인자들의 변화를 보면, 제 0일에 VOD군에서 PAI-1의 혈중 농도가 유의하게 더 높았다.(p=0.034). 결론: 재생불량성 빈혈환자에서도 조혈모세포 이식 후에 VOD의 높은 발생률을 관찰하였으며, 대부분이 경증이었다. VOD 환자에서의 혈액응고 인자들의 변화는 PAL-1이 VOD의 감별 진단을 위하여 이용될 수 있음을 시사하고 있다. Background: There are few reports about the occurrence of hepatic veno-occlusive disease(VOD) after bone marrow transplantation (BMT) for severe aplastic anemia (SAA). We analyzed the incidence and clinical outcomes of VOD and changes of coagulation factors in patients with SAA who were conditioned with cyclophosphamide plus ATG and underwent allogeneic BMT. Methods: Thirteen patients with SAA who underwent allogeneic BMT were included. A diagnosis of VOD was made according to clinical criteria, and severity of VOD was classified according to clinical course. Plasma levels of protein C, protein S, antithrombin Ⅲ, t-PA, and PAI-1 were determined before preparative chemotherapy, in the day of marrow infusion, and days 7, 14, and 21. Results: VOD occurred in 7/13(53.8%). Five were mild VOD and two were moderate VOD. Time to platelet ≥20,000/L was slower (p=0.026) and red cell and platelet transfusion requirements were higher (p=0.025,0.015) in patients with VOD . The mean plasma level of PAI-1 at 0 were higher in patients with VOD (p=0.034). Conclusion: We observed the high incidence of VOD after allogeneic BMT in patients with SAA. A majority of cases of VOD were mild. The changes of PAI-1 after BMT may be useful marker of hepatic VOD

골수이식 후 골밀도 및 골대사의 변화 : 2년 추적관찰 2-Year Prospective Study

이원영,강무일,오은숙,오기원,한제호,손현식,윤건호,차봉연,이광우,손호영,강성구,신완식,민우성,김춘추 대한내분비학회 2000 Endocrinology and metabolism Vol.15 No.4·5

다형성교아세포종을 동반한 Von Recklinghausen氏 병 1예

이현개,이종수,백민우,이승재,박효일,지제근 인제대학교 1981 仁濟醫學 Vol.2 No.4

가족성으로 신경섬유종증에 동반되는 중추신경계의 종양으로 수막종, 신경교종, 신경초종 등이 있으며 이들은 드물지 않게 보고되었다. 그러나 신경교세포에 생기는 종양 중 신경섬유종증에 동반되어 대뇌에 생긴 다형성교아세포종은 매우 드물다. 본 교실에서 가족성으로 발생한 신경섬유종증의 환자에 동반된 다형성교아세포종을 경험하였으며 가족중 부친은 von Recklinghausen씨 병을, 또 두 자녀는 다발성 cafe´ au lait spots를 가지고 있었다. The tumors of the central nervous system associated with familial neurofibromatosis are meningioma, glioma, and neurilemmoma, which are not uncommon. Of the glial tumors of the central nervous system, the association of cerebral glioblastoma multiforme with neurofibro matosis is very rare. We have experienced the unusual coincidence of neurofibromatosis and glioblastoma multiforme in a 33 year old man, whose father was afflicted with von Recklinghausen's disease and whose son and daughter had also multiple cafe´ au lait spots on their bodies.

초광대역 시간 호핑 PPM UWB 통신 시스템의 성능 평가

이민혁,전제훈,이성신,변건식 동아대학교 정보기술연구소 2003 情報技術硏究所論文誌 Vol.11 No.1

Ultra-wideband(UWB) communication applications is a recent innovation due to high transmission rate and low power emission. Therefore, it is important to research UWB singal's characteristics and modulation methods to apply to various UWB communication technologies. In this paper the characteristics of Gaussian monopulse which is capable of using UWB communication was analyzed and we compared the performance of PPM and PAM modulation method. Also we simulated the interference effect of QPSK system with different center frequencies in the present of various UWB noises and simply modeled UWB system.