알코올의 섭취가 흰쥐의 혈액과 간의 지질대사에 미치는 영향

이명숙,김영연,김효숙,김매하,전진순,서진숙,송선미,양영재,윤원손,이미라,이효영,정승현 誠信女子大學校 生活文化硏究所 2004 生活文化硏究 Vol.18 No.-

To investigate the effects of ethanol on the lipid metabolism in blood and liver, twenty one male Sprague Dawley rats had orally taken the 25% alcohol and 50% alcohol by 5g per Kg of weight during 3 weeks after the adjusting period. For the energy balance, 85% sucrose and 45% sucrose was added in control group and 25% alcohol group each. The weight was reduced by alcohol treatment according to the levels, otherwise the weight of liver and heart was little increased in alcohol groups rather than in control without significance. Moreover, RBC, WBC, hemoglobin and hematocrit were increased by alcohol treatment according to the levels without significance. Otherwise, adjusted bilirubin levels by 100g of weight was significantly increased by alcohol treatment dose dependently. Alcohol induced RBC degradation and increased circulated bilirubin because of liver damage. For blood lipid profiles, blood cholesterol was increased in 50% of alcohol group compared to that of 25% and control groups, otherwise, blood triglycerides (TG) was increased by 30% in 25% alcohol groups rather than in control but not significantly different. The hepatic lipid composition, cholesterol levels in alcohol treatment groups was lower than those in control. The hepatic TG was not influenced by alcohol treatment. The two reasons of decreasing hepatic lipids were suggested by 85% sucrose of control for energy balance more synthesized cholesterol and TG than those in 25 or 50% alcohol groups, and by cholesterol and TG could not be synthesized because of liver damage from alcohol.

쑥갓(Chrysanthemum coronarium L.)으로부터 배당체의 분리

송명종,김세영,송효남,백남인 경희대학교 생명자원과학연구원 2004 硏究論文集 Vol.23 No.-

쑥갓의 지상부를 MeOH로 추출하고, 추출물을 EtOAc, n-BuOH 및 물로 분배, 추출하였다. n-BuOH분획으로부터 silica gel 및 ODS column chromatography를 반복하여 배당체를 분리하였다. 화합물의 화학구조는, MNR 및 MS 등의 스펙트럼을 해석하여 1-o-β-D-glucopyranosyl-3-hexenol으로 동정하였다. The aerial parts of Garland (Chrysanthemum coronarium L.) were extracted in MeOH and solvent fractionated with EtOAc, n-BuOH and water, successively. n-BuOH fraction gave a glycoside compound through application of silica gel column chromatographies including ODS. The chemical structure of the glycoside was determined by the interpretation of several spectral data, including NMR and MS as O-β-D-glucopyranosyl-3-hexenol (1).

절수에 의한 Mongolian Gerbil 장기중량변화에 관한 분석연구

김무강,이기훈,이강이,송치원,이경열,권효정,박미선,정승혁,이행연,김명철 충남대학교 수의과대학 동물의과학연구소 2000 動物醫科學硏究誌 Vol.8 No.-

In this study, authors measured the each organ weight of the long term water deprived Mongolian gerbil, after then we calculated the mean, standard deviation, variance coefficient of the real measured organ weight and induced the organ weight change quantity, rate of quantity, deviation between measured and theological organ weight, deviation rate, sum of deviation rate, organ weight change rate, rate of organ weight by the mathematical formula. The results obtained as follow 1. The weight of the brains, lung, and testis were abruptly decreased after water deprived until the 5th day, after then slowly decreased until the 20th day. 2. The weight of the heart was decreased gradually from the first day until the 20th day. 3. The weight of the liver was abruptly decreased until the 10th day, after then the more slowly decreased until the 20th day. 4. The weight of the thymus, spleen, kidney, and pancreas were abruptly decreased until the 15th day, after then increased slowly until 20th day but the weight of the kidney was abruptly increased from the 10th day. 5. The organ weight change rate and the rate of the each day organ weight were very diversified.

肝癌株와 S-180에 對한 茵蔯 分劃의 抗腫瘍效果

송효정,김성훈,김진웅 대한동의병리학회 1995 동의생리병리학회지 Vol.9 No.2

연구배경 :최근 다용되는 항암제가 여러 부작용과 면역기능저하등의 문제점이 대두 되며서 이에 대한 대책으로 생약 또는 한약축출물을 기존의 항암제와 병용하여 치료효과를 높이는데 많은 연구가 이루어지고 있는데 본 논문도 위암에 이어 두 번째의 사망률을 점하고 있는 간암에 대하여 간계질환에 널리 쓰이는 인진을 이용하여 항암 효과 및 항암제와의 병용 효과를 실험을 통하여 규명하여 실제 임상에 있어 간암 치료에 도움이 되고자 함. 연구방법 : In vitro에서 茵陳을 water, hexane, ethyl ether, ethyl acetate로 抽出한 分劃의 抗腫瘍 效果 및 抗癌劑 adriamycin(ARM), cisplatin(CPT), 5-fluo-rouracil (5-FU) 등과의 相乘作用을 MTT 法에 의해 살펴 보고, 動物 實驗에서는 ICR 생쥐에 S-180을 腹腔 注射한 後 i.p로 名層의 엑기스를 投入하여 體重變化, 生命延長率, 및 血液檢査 등을 實施함 연구결과 : 茵陳 分劃의 肝癌株에 대한 抗腫瘍 效果가 認定되나 癌株는 Hep G2, 分劃은 water層과 ethyl ether層이, 相乘 效果는 Adriamycin과의 倂用 投與에서보다 效果的인 것으로 나타났다. In order to prove the antitumor effect of fraction of Herba Artemisiae Capillaris experimentally, studies were done. The antitumor effect of fractions of Herba Artemisiae Capillaris against hepatic cancer cells such as Hep G2, Hep 3B & PLC, also the synergstric action were evaluated in the combined treatment with anticancer drugs using chiefly for the treatment of liver by measuring body weight, survival time, hematological changes in ICR bearing S-180. The results were obtained as follows: 1. In cytotoxic effect against Hep G2, inhibitory growth rate below 55% of control was recognized in concentration of 10-3g/ml of water, ethyl ether layer. the synergistic effect with adriamycin was shown in concentration of 10-5g/ml above in water layer, 10-5 g/ml above in hexane layer, 10-4g/ml above in ethyl ether layer, the synergistic effect with cisplatin was shown in concentration of of 10-5g/ml above in water layer, 10-3g/ml in ether layer, the synergistic effect with 5-fluorouracil was shown in concentration of 10-3g/ml in water, ethyl ether layer. 2. In cytotoxic effect against PLC, the concentrations showing inhibitory growth rate below 55% of control was concentration of 10-4g/ml above in water, ethyl ether layer, the synergistic effect with adriamycin was shown in concentration of 10-4g/ml above in water, ethyl ether layer, the synergistic effect with cisplatin was shown in concen-tration of 10-5g/ml above in water layer, ethyl ether layer, 10-3g/ml in hexane, ethyl acetate layer, the synergistic effect with 5-fluorouracil(5-FU) was shown in concentration of 10-3g/ml in water layer. 3. In cytotoxic effect against Hep 3B, inhibitory growth rate below 55% of control was concentration of 10-3g/ml in water layer. the synergistic effect with adriamycin was shown in concentration of 10-3g/ml in ether layer, 10-4g/ml above in water layer, hexane layer, the synergistic effect with cisplatin was shown in concentration of 10-4g/ml above in water layer, the synergistic effect with 5-fluorouracil(5-FU) was shown in concentration of 10-3g/ml in water, ethyl acetate layer 4. In vivo, the gaining of body weight were significantly suppressed in water of Herba Artemisiae Capillaris only as compared with control group bearing S-180 and ILS was 18.2% in water layer, 16% in hexane layer, 17% in ethyl ether layer respectively. 5. The number of platelet was significantly increased in water layer, ethyl ether layer and platelet aggregation was significantly increased in waster, hexane, ethyl ethers and ethyl acetate layer as compared with control group. From the above result it was concluded that fractions of Herba Artemisiae Capillaris had antitumor effect, but Hep G2 of human hepatic tumor cells, water, ethyl ether layer of all fractions and adriamycin of anticancer drugs were more effective in the antitiumor activity.

桔梗湯과 加味桔梗湯의 抗癌效果와 免疫反應에 關한 硏究

宋孝貞,金聖勳,李光浩 대한동의병리학회 1996 동의생리병리학회지 Vol.10 No.1

肺癰에 使用되고 있는 桔梗湯을, 肺癌株 및 S-180에 投與하여 抗癌 抗轉移 및 免疫效果를 實驗的으로 糾明하고, 여기에 現在 肺癌에 多用되고 있는 2가지 藥物을 加味하여 相乘效果를 觀察하고자 하였다. C57BL/6에 B16-Fo를 皮下主射하고 ICR생쥐에 S-180을 腹腔 注射한 後 桔梗湯과 加味桔梗湯을 投與하여 腫瘍重量과 體重變化, 生命延長率, 組織變化 및 血液學的變化 등을 測定하여 抗癌效果를 分析하고, 아울러 FACS에 의해 BALB/c의 脾臟免疫細胞에 대한 影響을 살펴보았다. 桔梗湯, 桔梗湯加腥草 및 桔梗湯加白花蛇舌草등은 모두 抗癌, 抗轉移 및 免疫反應에서 有意性있는 結果를 認定되었으며, 魚腥草나 白花蛇舌草를 加味하였을 경우 더욱 效果的으로 나타났다. In order to prove the antitumor effect of Kilkyungtang(KKT), Kilkyungtang with Houttuyniae Herba(KKT-I) and Kilkyungtang with Oldenlandiae diffusae Herba(KKT-Ⅱ) experimentally, the studies were done. We measured tumor mass and body weight hematological change in ICR bearing S-180 and C57BL/6 implanted with B16-Fo, also studied their effect on immune cells in BALB/c by FACS. The results were obtained as follows: 1. Body and tumor weight were significantly suppressed in all groups as compared with control group. MST(mean survival time) was 11% in KKT, 16.9% in KKT-Ⅰ, 16.1% in KKT-Ⅱ for 14 days after implantation s.c. with B16-Fo and also body weight was significantly suppressed after implantation i.p. with S-180, MST was 20.1% in KKT, 40.9% in KKT-1, 56.6% KKT-Ⅱ for 62 days. 2. WBC and platelet aggregation was significantly increased in all groups, the number of platelet was significantly increased in KKT and KKT-Ⅰ as compared with control group. 3. The infiltration of tumor cell was obvious in the white and red pulps, in the sinusoid of liver and the glomeluli of kidneys after implantation with S-180, whereas the changes of tissues were not significant in KKT-Ⅱ, KKT-Ⅰ and KKT in order. 4. T cell and T helper cell were increased in KKT-Ⅰ and KKT-Ⅱ treated groups and B cell was increased in KKT treated group as compared with control group. From above results it was concluded that Kilkyungtang(KKT), kilkyungtang with Houttuyniae Herba(KKT-Ⅰ) and Kilkyungtang with Oldenlandiae diffusae Herba(KKT- Ⅱ) had antitumor effect, and also Kilkyungtang combined with Houttuyniae Herba or Odenlandiae diffusae Herba were more effective than KKT only which suggests the necessity of continuous study on the effects of Houttuyniae Herba or Oldenlandiae diffusae Herba.

李東垣의 陰火論과 朱丹溪의 相火論의 比較硏究(Ⅱ)

宋孝貞,金聖勳,金東熙,宋旭 대한동의병리학회 1998 동의생리병리학회지 Vol.12 No.2

火에 대한 槪念은 內經에서 言及한 이래 生理, 病理, 運氣的인 側面에서 여러 醫家들에 의해 多樣하게 解析되어지고 있다. 특히 古來의 理論에 얽메이지 않고 豊富한 臨床經驗을 바탕으로 李東垣은 脾胃論의 主要內容으로 '陰火論'를 主張 하였고, 朱丹溪는 歷代 醫家들의 火에 對한 槪念을 整理하여 '相火論'과 '陽常有餘 陰常不足論'을 主張하여 모두 獨特한 火의 理論을 集大成하였다. 後代에 命門學說이 나오기까지 重要한 理論·臨床的 資料를 提示하여 明代의 溫補派와 滋陰派로 集約되어 繼承되어진 東垣의 陰火와 丹溪의 相火에 대하여 歷史·環境的 背景, 槪念, 生理, 病理 및 治法 等을 諸文獻을 通하여 比較 檢討하였다. Through studing historical and environmental background, definition, physiology, pathology and therapy of Li DongWon' theory, Yin-fire, and Zhu DanXi's theory, ministerial fire, the conclusions were obtained as follows. 1. Yin-fire was a expression of debilitating heat induced by shortage of primordial Qi, even though excessive syndrome came out. 2. Ministerial fire is based on the motive power of bowel visceral activity and maintenances of life in physiology. But it is also the noxious factor that means it is the enemy of primordial Qi in pathology. 3. The etiology and pathology of Yin-fire was explained by the deficiency of spleen and stomach's vital energy caused by irregular food, overstrains and emotional stress. 4. It was thought that ministerial fire was caused by excess of emotion, fatty food and sexual intercourse and among theses factors (that gave rise to fire of bowel, visceral and Osungguelyang(五性厥陽), excessive emotion was the most important part. 5. The syndrome of yin-fire were consisted of two groups, ie. damp heat and deficiency of vital energy syndrome. And the syndrome of pathological ministerial fire is similar with the syndrome of fire among Nei-Ching's 19 pathologies, which is rapid and various. 6. Dong-Won emphasized the treating method of supplementing digestive tract and ascending yang by using sweat and warm herbs according symptom. In the other hand, DAn-Xi emphasized the treating method of nourishing yin and relieving fire by using bitter and cold herbs.

ECT (European Compression Technic) System 을 사용한 下顎骨 骨折의 治驗例

金晤煥,申曉根,宋采炫,崔秉河,權泰鎬 全北大學校 齒醫學硏究所 1983 전북치대논문집 Vol.1 No.1

The authors in the Dept. of Oral & Maxillo-facial Surgery.J.N.U Hospital could have the following results after treatment of eight mandibular fractures patients with the E.C.T.(European Compression Technic)System. 1. Accurate reduction of the fractured bone. 2. Minimization of the soft tissue injury during operations. 3. No post-operative intermaxillary fixation was necessary. 4. Good recovery of the occlusion.

Myopic Bounds for the Random Yield and Nonstationary Stochastic Leadtime Demand Problem

Kim, Keun-Chong,Song, Young-Hyo The Institute of Management Hong-ik University 2001 Hongik business review Vol.4 No.-

The purpose of the current paper is to combine the results of Morton and Pentico^3) Anupindi et al.^4) for the nonstationary stochastic leadtime inventory problem with recent works of Kim^12,13) which considered the production capacity, random yields, and nonstationary demand, and a production planning model under JIT delivery contracts. We can obtain myopic, and near-myopic heuristic upper and lower bounds for the case of nonstationary stochastic leadtime demands with random yields due to the variable production capacity.

금병산과 적오산의 산림군락 분류

김효정,이미정,지윤의,이규석,송호경 忠南大學校 環境問題硏究所 2002 環境硏究 Vol.20 No.-

대전에 위치한 금병산과 적오산의 식생을 분류하기 위하여 연구가 수행되었다. 총 41개의 조사구에서 식물사회학적 방법으로 조사를 하였다. 이 지역 산림식생은 크게 이차림식생과 조림식생으로 구분 되었다. 이차림 식생은 소나무군락, 상수리나무군락, 팽나무군락으로 구분되었다. 조림식생은 아까시나무군락, 리기다소나무군락, 일본잎갈나무군락, 곰솔군락으로 구분되었다. 본 조사지역에서 출현한 종수는 총 145종이었으며, 가중나무, 개망초, 미국자리공 아까시나무, 족제비싸리 등 총 5종의 귀화식물이 출현하였다. This study was carried out to classify forest vegetation community of Geumbyeongsan and Jeokosan in Daejeon. Employing the releve´ method of Dierssen, 41 plots were sampled. Forest community ws classified as second forest vegetation and afforestation vegetation. The second forest vegetation was classified into six communities such as Pinus densiflora community, Quercus acutissima community, Quercus serrata community, Quercus variabilis community, Quercus aliena community, and Celtis sinensis community. Afforestation vegetation was classified again as Robinia pseudoacacia community, Pinus rigida community, Larix leptolepis community, and Pinus thunbergii community. In the present study area, we found a total of 145 taxa. We found a total of five naturalized plants taxa of Ailanthus altissima, Erigeron annuus, Phytolacca americana, Robinia pseudoacacia, and Amorpha fruticosa.

단독주택의 용도별 에너지 소비에 관한 연구

김병수,장문석,박효순,송인춘 충남대학교 산업기술연구소 2000 산업기술연구논문집 Vol.15 No.1

The purpose of this study is to analyze the situation of energy consumption and its characteristics in detached houses. The survey is carried out in detached houses to investigate the energy consumption and energy use trend in according to three different climate zones. Building energy simulation by DOE-2 computer program was undertaken for detailed analysis of the energy use characteristic in detached houses. Also, The room temperature and the energy use of the year were also calculated. Finally, The energy consumption characteristics of heating, cooling, cooking and electric equipments in detached houses depends on life style of resident such as room temperature, occupancy type and equipment operating schedule and etc. And also, the results of this study will be to provide basic data concerned with total energy consumption in detached houses.