Generation of Transgenic Pigs Carrying a Construct for Endothelial Specific Expression of CD73

Keon Bong Oh,Bella Kim,Nayoung Ko,Dae-Jin Kwon,Dong-Hoon Kim,Gi-Sun Im,Jin-Ki Park,Seongsoo Hwang 한국동물생명공학회(구 한국동물번식학회) 2011 발생공학 국제심포지엄 및 학술대회 Vol.2011 No.1

Despite of the absence of hyperacute rejection and acute humoral xenograft rejection, the organ graft of the a1,3-galactosyltransferase (GalT) gene knockouted (KO) and complement regulatory protein (CRP) expressing pig into a nonhuman primate is rejected by development of a thrombotic microangiopathy and/or a consumptive coagulopathy. Thus further introduction of genes to overcome the coagulation incompatibilities between pig and primate under GalT KO/CRP genetic background has been strongly suggested. CD73 (ecto-5'-nucelotidase) is an enzyme attached via a glycosyl phosphoinositol anchor to the extracellular membrane of endothelial cells, which catalyses the hydrolysis of adenosine triphosphate to adenosine. Loss of activity of CD73 results in activation and aggregation of platelets by a reduced capacity to convert nucleotides to adenosine. In previous study, we reported generation of GalT KO fibroblasts concurrently expressing membrane cofactor protein and produced cloned pigs by nuclear transfer of the fibroblast cells (1). In this study, we constructed a vector for expression of human CD73 under control of promoter of pig Icam2 gene expressed specifically at endothelial cells. This vector was introduced into porcine fibroblasts using the nucleofection technology, by which we had forty three fibroblasts clones carrying pIcam2- CD73 vector. Somatic cell nuclear transfer resulted in generation of two transgenic piglets survived.

패밀리레스토랑 종업원의 위생과 청결에 관한 중요도 인식

고호석,김선경,김동기,김범진 한국조리과학회 2005 한국식품조리과학회지 Vol.21 No.2

The purpose of this study was to identify factors associated with sanitation and cleanliness regarding the importance of family restaurant employees in the Ulsan and Basin areas. Accordingly, this study conducted a survey questionnaire consisting of 28 measures of food-service as well as major subject descriptors. From the results, the frequency, descriptive, factor, correspondence analysis, major findings and marketing implications could be summarized as follows: First, 6 factors were extracted: cooking and control factor, food and tableware handling factor, personal cleanliness condition factor, purchase and receiving factor, unit food material store factor and Kitchen utensil sanitation factor. KMO and Bartlett'stest statistics showed that the data fitted the factor analysis well. Results of factor analysis, average variance extracted estimates and shared variance showed that the convergent and discriminant validity of 6 factors are supported and Cronhach's alpha showed that the internal consistency of the 6 factors was supported. Second, correspondence analysis indicated that statistically significant relationships existed between some of the family restaurant employee sanitation and cleanliness factors and the family restaurant enterprise.

동종 조혈모세포이식 후 발생한 크립토콕쿠스 척추염 1예

고윤호,임동준,이성수,조유경,이동건,최정현,김유진,민창기,김동욱,박정미,김춘추,신완식 대한감염학회 2001 감염 Vol.33 No.4

Skeletal cryptococcosis is an uncommon infection. Cryptococcus is a common cause of meningitis and infects 7∼10% of patients with AIDS. As well as AIDS, the infection may be seen in association with leukemia, lymphoma, Hodgkin's disease, sarcoidosis, tuberculosis and diabetes, also in patients on steroid medication. But there is no case report of skeletal cryptococcosis following allogeneic hematopoietic stem cell transplantation. A 40-year-old woman was admitted to the hospital because of low back pain. She had chronic myelogenous leukemia for 2 years and underwent allogeneic hematopoietic stem cell transplantation 8 months ago. She have been treated with steroid and cyclosporine orally because of chronic graft versus host disease. On examination she was afebrile and had posterior lower lumbar tenderness. But, she had no reduced strength of low extremities. Open biopsy was underwent. Histology demonstrated budding, round-to-oval, refractile yeast-like organisms within debris. The results of a lumbar puncture were unremarkable and cerebrospinal fluid culture failed to grow bacteria and yeast. The patient was treated with amphotericin B (1 gram) and AmBisome□ (2.8 gram) over 6 weeks. Three months after cessation of therapy, the patient was doing well.(Korean J Infect Dis 33:298∼301, 2001)

들깻잎이 재배 및 저장기간중 Procymidone 및 Bifenthrin의 잔류량변화

고광용,이용재,원동준,박혜진,이규승 한국환경농학회 2003 한국환경농학회지 Vol.22 No.1

들깻잎에서 procymidone과 bifenthrin의 경우 안전사용기준인 약제살포 후 5일에 각각의 농도가 38.16, 1.77 mg/kg 이지만 이는 살포 조건과 재배환경에 따른 농약의 초기부착농도가 높은데서 기인한 것이라고 예상되어진다. 앞에서의 결과들로 미루어 볼 때 일반적인 농약의 안전사용기준으로 농약을 처리한다면 최초살포 이후 재배기간에서 5일간 50% 이상 이 분해되고 10일 후에는 90%이상이 분해된다. 또한 상온저장 중 20~40%가 분해되며 수돗물 세척에 의해서 만으로도 50%이상이 제거되므로 조리과정을 감안하지 않더라도 초기살포량의 약 90%는 분해 또는 제거되어 안전사용기준에 의거하여 사용한다면 두 약제 모두 MRL이하로 잔류하게 되어 별다른 문제가 없을 것으로 예상되어진다. 하지만 현행법상 수확 시점에 그 잔류량을 조사하도록 되어 있으므로 깻잎의 경우 재배가 대부분 하우스에서 이루어짐을 감안한다면 두 약제 모두 초기살포시 작물체로서의 부착량이 많을 경우에 수확시점에서의 잔류량이 MRL을 상회할 가능성도 있음을 알 수 있었다. 따라서 이에 따른 약제 사용량의 축소나 저농도 약제의 사용, 살포일수의 조정 등에 관한 연구가 필요하다고 본다. In order to know residual pattern of pesticides and to predict degradation period until below MRL, we experimented with procymidone and bifenthrin for perlua leaf which were the most detected pesticides by NAQS (National Agriculture-product Quality management Service) survey. In this experiment, we sprayed those pesticides 10 days before harvest and analyzed 0, 1, 2, 3, 5, 7, 10 day samples to establish logical equation and to calculate DT_(50). Also degradation patterns of those samples were compared during storage at 4℃ and 20℃. During cultivating period, procymidone residue amount was changed from 79.52 mg/kg (0 day) to 4.2 mg/kg, DT_(50) was 2.65 days by logical-equation, and bifenthrin residue amount was changed from 5.03 mg/kg (0 day) to 0.17 mg/kg, DT_(50) was 2.24 days. During storage period, DTso of procymidone and bifenthrin at 4℃ was 12.23 days and 10.57 days, and at 20℃ was 6.32 days and 8.2 days, respectively.

중국 스포츠 산업의 현황과 발전전망

조동진,고철 朝鮮大學校 스포츠科學硏究所 2006 스포츠科學硏究 Vol.17 No.-

The purpose of this study was to examine the situations and problems found in the course of development of the sports industry in China, to analyze its future prospect, and to explore development tasks. For study instrument, literature review and Internet search was used to integrate and analyze Korea- and China-related data to be fit for the purpose of this study. The results of this study are as follows. First, China had no systematic policy as well as systems in the sports industry. Second, the Beijing Olympics is a good opportunity to develop the sports industry and will provide development possibility. Accordingly, this study suggests the followings for the development of the sports industry in China. 1) The distinctive sports industry fit for local color should be developed. 2) With the complete and advanced policy of the sports industry, policy needed to develop the sports industry should be provided. 3) The management level of the sports industry should be improved by cultivating capable people of the sports industry. 4) The global trend of the sports industry should be understood and forecasted.

숯 처리에 의한 녹차 추출액 중 질산태질소 제거 효과

김진우,고영옥,신동국,이 협,전현식,이종옥 진주산업대학교 산업과학기술연구소 2005 산업과학기술연구소보 Vol.- No.12

This experiment was carried out to measure the elimination effect of nitrate nitrogen from green tea extracts through the column system filled with charcoal activated and bamboo charcoal powder compare to that of an nitrate nitrogen standard solution prepared with KNO3 chemicals. The change patterns of pH, and the amount of removed nitrate nitrogen from green tea and nitrate nitrogen standard solution were analyzed according to time course and the amount of charcoal treatments. The results were summarized as follow: 1. pH The pH of Green tea extractions treated with charcoal was high up to 8.17(after 30min treated with 0.4% charcoal) and 8.36(after 60min with 0.4% charcoal) which pH of non-treated green tea (control) was 5.8 The results indicated that the pH values were variable to the treated amount of charcoal and the teated time course. 2. Nitrate nitrogen From the nitrate nitrogen standard solution, the least rate 69.1% and the highest rate 74.5% of nitrate nitrogen were observed to be absorbed through the column treatment (60 min), which with green tea extract solution 21.3% (the least) and 43.8% (the highest) were detected to be absorbed through column treatment (after 60min with 0.4% charcoal). In the case of bamboo treatments with nitrate nitrogen solution samples were showed the least rate 62.8% (30min with 0.1% bamboo) and the highest rate 68.9% (60min with 0.4% bamboo), and with green tea extract solution were observed the least 13.6 (30min with 0.1% bamboo) the highest rate 29.4% (60min with 0.4% bamboo). In general, the elimination activity of nitrate nitrogen was higher by the charcoal activated powder treatment than bamboo charcoal.

선천 섬유화 증후군 1예

김동섭,신정철,고명규,최준규,장세진 한양대학교 의과대학 1998 한양의대 학술지 Vol.18 No.1

The congential fibrosis syndorme is rare congenital disorder which is characterized by blepharoptosis, varying degrees of limitation of eye movement, especially in vertical gaze, and abnormal head position. These clinical findings result from the fibrous replacement of normal contractile extraocular muscle tissue. The authors report one sporadic case of congential fibrosis syndrome. The 14 year-old-girl showed bilateral ptosis, fixed ocular misalignment in downward gaze, limited ductions and versions, and characteristic chin up position. The surgical management attempted to achieve functional readjustment of the ocular alignment and lid position and to correct cosmetically unacceptable abnormal head position. Result of histopathologic examination obtained at surgery shows replacement of the extraocular muscles, conjuctiva and Tenon's capsule by collagen and dense fibrous tissue. The surgical outcome is considered satisfactory when compared with preoperative status although the limitation of vertical eye movement remains unchanged after surgery.

X-선 조사를 받은 흰쥐망막 빛감각세포의 미세구조

고정식,박동분,양남길,안의태,박경호,김진국 대한해부학회 1993 Anatomy & Cell Biology Vol.26 No.3

정상 월경주기에 따른 혈액 응고 기능의 변화 : 혈전탄성묘사도를 이용한 평가

김동찬,이진,고성훈,이준례,송희선 대한마취과학회 2002 Korean Journal of Anesthesiology Vol.43 No.4

Background: As the menstruation has been thought to be associated with abnormal hemostasis and increased fibrinolytic activities, some surgeons and even anesthesiologists tend to postpone elective surgery. Many investigators have studied individual coagulation profiles and fibrinolytic activity during the menstruation, but conventional coagulation screens are frequently inadequate for the purpose of monitoring coagulation dynamically. This study was designed to evaluate the whole dynamic hemostatic process globally during the normal menstrual cycle by the thromboelastography (TEG). Methods: Thirty six-healthy, normally menstruating female volunteers wrote a menstruation diary about their menstruation cycle. Blood samples were obtained for TEG analysis 3 days before menstruation, the 1st day of menstruation, the 2nd day of menstruation, the last day of menstruation and 5days after menstruation. TEG coagulation indices (R value, K value, α angle, WBCL160 during menstruation compared with pre- or post-menstruation period, but there was a significant decrease in the MA value on the 1st day of the menstruation. Conclusions: The result of our study showed the possibility of abnormal hemostatic function on the 1st day of menstruation. However, the more study is needed to confirm the possibility of bleeding tendency during surgery on the 1st day of menstruation. (Korean J Anesthesiol 2002; 43: 413~417)

오렌지로부터 아플라톡신B₁의 정량분석에 관한 연구

장향동,백광균,구경림,옥선미,최진석,고은주,류성렬 한국공업화학회 2004 응용화학 Vol.8 No.2

Aflatoxin B₁ was known to have the strongest poisonous character among Aflatoxin compounds. To search the production of Aflatoxin B1 according to the change of temperature, relative humidity and incubation days, orange incubated with Aspergillus parasiticus KCCM 35078 was incubated at 20℃, 25℃, 30℃ at relative humidity 60%, 70%, 80% and for incubation time 3, 4, 5, 6, 7 days.. Production of Aflatoxin B₁ shows that the highest production appeared at 25℃ and its relative humidity 70%. The result that was quantitatively analyzed by using HPLC was 77.41ppm, and by using Fluoroscence detector was 87.87ppm. - Production of Aflatoxin B₁ shows that the lowest production appreared at 20℃ and its relative humidity 80%(HPLC), 60%(Fluoroscence detector). The result that was quantitatively analyzed by using HPLC was 4.47ppm, and by using Fluoroscence detector was 4.17ppm. The structure of Aflatoxin B₁ was identify with authentic sample by using instrumental analysis methods of GC/MS, ¹H-NMR, FT-IR respectively.