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정향동,원옥남,구경림,류성렬 한국공업화학회 2003 응용화학 Vol.7 No.2
Aflatoxin B₁was known to have the strongest poisonous character among Aflatoxn compounds. To search the production of Aflatoxin B₁according to the change of temperature and relative humidity, millet incubated with Aspergillus flavus KCCM 35078 was incubated at 20℃, 25℃, 30℃, 25℃ and at relative humidity 50%, 60%, 70% and for incubation time 72, 96, 120, 144, 168, 192 hours. Production of Aflatoxin B₁shows that the highest production appeared at 25℃ and its relative humidity 70%. The result that was quantitatively analyzed by using HPLC was 23.35ppm, and by using fluorescence detector was 23.50ppm Production of Aflatoxin B₁shows that the lowed production appeared at 20℃ and its relative humidity 50%. The result that was Quantitatively analyzed by using HPLC was 6,26ppm, and by using fluorescence detector was 6.33ppm The structure of Aflatoxin B₁was identify with authentic sample by using instrumental analysis methods of MS, H-NMR, FT-IR, respectively.
장향동,백광균,구경림,옥선미,최진석,고은주,류성렬 한국공업화학회 2004 응용화학 Vol.8 No.2
Aflatoxin B₁ was known to have the strongest poisonous character among Aflatoxin compounds. To search the production of Aflatoxin B1 according to the change of temperature, relative humidity and incubation days, orange incubated with Aspergillus parasiticus KCCM 35078 was incubated at 20℃, 25℃, 30℃ at relative humidity 60%, 70%, 80% and for incubation time 3, 4, 5, 6, 7 days.. Production of Aflatoxin B₁ shows that the highest production appeared at 25℃ and its relative humidity 70%. The result that was quantitatively analyzed by using HPLC was 77.41ppm, and by using Fluoroscence detector was 87.87ppm. - Production of Aflatoxin B₁ shows that the lowest production appreared at 20℃ and its relative humidity 80%(HPLC), 60%(Fluoroscence detector). The result that was quantitatively analyzed by using HPLC was 4.47ppm, and by using Fluoroscence detector was 4.17ppm. The structure of Aflatoxin B₁ was identify with authentic sample by using instrumental analysis methods of GC/MS, ¹H-NMR, FT-IR respectively.