성인에서 장기간 Furosemide오용과 연관된 신수질 석회화

김윤구,이윤하,이규백,장세호,김대중,오하영,김보현,김미경,조윤숙 대한신장학회 1996 대한신장학회잡지 Vol.15 No.4

Three-Dimensional Z-Plasty(Yoon's Method)를 이용한 내안각 췌피 성형술

박흥식,김우신,윤진호,나민화,김한중 대한미용성형외과학회 1997 Archives of Aesthetic Plastic Surgery Vol.3 No.1

The presence of epicanthal folds in Asian eyelids is one of their unique features, in combination with the lack of supratarsal folds. Western culture has influenced many people to prefer to eliminate the prominent epicanthal fold. A number of surgical techniques have been suggested for their correction. However, difficulty with design, excessive and prominent scarring of medial canthal and nasal area, recurrence, and rigidity of application are potential problems associated with many procedures. This paper describes a epicanthoplasty with three-dimensional Z-plasty. Through epicanthal fold incision, the medial canthal tendon is medially advanced and sutured to the periosteum of nasal bone. After the transposition of the flaps, trimming of the flap is usually required. Three-dimensional Z-plasty creates the attractive eyes. From March, 1995 to March, 1997, the technique was applied to 37 patients with epicanthal fold and was performed with or without double-fold operation. There were 34 females and 3 males with ages ranging from 17 to 30 years. Through 2 years follow-up, this technique has delivered esthetically good results with minimal postoperative scar and could made the reduction of ICD from 40 ± 2.13 mm to 34 ± 1.98 mm. The advantages of epicanthoplasty procedure using three-dimensional Z-plasty(Yoon's method) are as follows; 1) simple in design 2)minimal postoperative scar in the medial canthal area 3) versatile in its application 4) no recurrence 5) no hypertrophic scar 6) preserving ethnic identity.

현행 중등학교 과학 실험 , 실습 교육 실태 조사 및 그 운영 진단 (Ⅱ) : 고등학교 과학 실험 , 실습 교육을 중심으로

김영호,이윤종,정원우,강용희,양승영,윤성효,안병호,윤일희,권용주,임성규,오철한,기우항,전명남,김중욱 한국과학교육학회 1998 한국과학교육학회지 Vol.18 No.3

본 연구는 96년도에 실시한 우리 나라 중학교 과학 실험·실습 교육의 실태 조사 연구에 따른 계속 연구의 일환으로 고등학교 과학 실험·실습 교육의 실태를 조사하기 위한 것이다. 연구내용은 고등학교 과학 교과서의 분석, 실험·실습 실시 현황 분석, 현행 실험·실습 교육의 문제점 분석, 현행 학교 구성원들의 실험·실습 교육 운영 실태 조사 및 현행 실험·실습 교육의 개선을 위한 연구과제 도출을 주요 연구내용으로 다루었다. 연구방법은 전국의 고등학교 80개교를 대상으로 이들 학교의 학생 1,977명, 교사 165명, 학교장 80명에게 적용하였다. 본 연구에서 밝혀진 결과를 중심으로 우리 나라 고등학교 과학 실험 실습 교육의 정상화를 위한 실험 내적인 연구과제를 제시하면 다음과 같다. (1)교재 개발에 대한 연구, (2)실험 킷트 개발에 대한 연구, (3)교사교육 및 재교육 프로그램 개발에 대한 연구, (4)교실 밖 실험·실습 활동 프로그램 개발에 대한 연구, (5)과학 실험·실습 소재의 개발에 대한 연구. This study was accomplished to analysis and survey on the experimental and practical science education of high school in korea for the consecutive study of the an analysis and survey on the experimental and practical science education of middle school in korea(Lee, Yoon-Jong et al., 1997). The status of facilities, management for the experiment, practices, teaching methods in high schools have been investigated. The present status and reasonable management of the high school science education have been grasped from the questionaires. To do this 165 high school science teachers, 1977 students and 80 principals of high schools in Korea are administered questionaires of Science Education Research Institute of Kyungpook National University(1997). The results of this study are as follows : The reasonable management for experiments and practices of science education were scanty in the high school around the urban and rural school owing to the shortage of facilities and equipments, crowded class, excessive class works for teacher, excessive contents of present textbooks and insufficiency of the administrative supports etc. The current teaching method of high school science has emphasized knowledge. This fact does not satisfy the objective of learning due to lack of the teaching method. Desirable directions for the improvement of present status of high school science education were proposed in this paper.

複合 韓藥劑 淸籬滋坎湯의 抗癌效果 및 作用機轉 糾明

Cho, Kyung-Sam,Kim, Si-Young,Park, Jai-Kyung,Choi, Seung-Hoon,Chung, Se-Young,Yoon, Hwi-Joong 경희대학교 동서의학연구소 1999 INTERNATIONAL SYMPOSIUM ON EAST-WEST MEDICINE Vol.1999 No.1

Kyung Sam Cho¹Si Young Kim¹, Jai Kyung Park²,Seung Hoon Choi³,Se Young Chung⁴, Hwi Joong Yoon¹¹College of Medicine, ²East-West Medical Research Institute, ³College of Oriental Medicine, ⁴College of Pharmacology, Kyung Hee University,Seoul, Korea. The Anti-cancer Effect of Oriental Medicine Chung-Ri-Ja-Gam-Tang in the Mouse with Metastatic Lung Cancer. Proceedings of International Symposium on East-West Medicine, Seoul. 231-243, 1999.-The oriental medicine Chung-Ri-Ja-Gam-Tang is an herbal medicine which has been used for pulmonary diseases and known as having immune stimulatory effects. It has been known effective in lung cancer. So we studied the effects and the mechanisms of this herbal medicine it the mouse with metastatic lung cancer. The metastatic lung cancer of the mouse was produced with melnoma cell line(B16BL/6).5×10□/mouse tumor cells were injected intravenously to the CDF1 mouse via tail vein. The mice were divided 4 groups. The first group was treated with 50mg/kg extract of Chung-Ri-Ja-Gam-Tang per oral for 10 days after cancer cell injection, second group treated with saline after cancer cell injection, the third group with medicine without cancer cell, and the last group with saline only. After 10 days treatments some of the mice were scarificed and the lung and spleen was removed. The survival duration, weight change, the number of metastatic cancer nodule of the lung, the NK cell activity, the capacity of cytokines(INF-γ,INF-α,IL-2) production and the proliferation activities of mouse lymphocytes were measured. The surival times of the group 1 mice were longer and the weight loss was less than the group 2 significantly. The number of the metastatic nodule of the lung were decresed in the group 1 than the group 2. The production of INF-γ,was increase in group 2 than group 3 and 4, IL-2 production was increased in group 1 than group 2,3,4 significantly. There was no difference in TNF- α production and proliferation activity of lymphocyte in each group. The NK cell activity was significantly increased in group 1 than group 2,3,4. We conclude that the Chung-Ri-Ja-Gam-Tang has the effect of increasing the NK cell activity of the CDF1 mouse with metastatic lung cancer(B16BL/6). And it is suggested that the increased production of the IL-2 is the mechanisms of enhanced NK cell activity.

갑상선 전절제술 및 중심 경부 림프절 절제술에 따른 혈중칼슘치 저하에 대한 연구

고중화,안성윤,김휘준,신지철 대한이비인후과학회 1999 대한이비인후과학회지 두경부외과학 Vol.42 No.12

Loop-mediated Isothermal Amplification(LAMP) 법을 이용한 Kakugo Virus의 검출법 개발

이중구,윤병수 경기대학교 사회과학연구소 2012 경기대학교 기초과학논문집 Vol.25 No.1

Kakugo Virus(KV) is a picorna-like virus that was originally identified in the brains of aggressive but apparently healthy worker honeybees(Apis mellifera L.). A loop-mediated isothermal amplification (LAMP) assay allows one-step detection of gene amplification without expensive equipment such as thermocycler. In this study, new LAMP method for the specific detection of KV in honeybee was developed (KV-LAMP). A set of four designed primers named KV-F3 / B3 / FIP / BIP that recognize KV sepcific sequence(GenBank, 250362.1). After optimization of conditions, KV-LAMP specific amplification was successfully performed using KV-LAMP from standard DNA sequence. KV-specific template was amplified under isothermal conditions at 59℃ within 60 minutes by KV-LAMP. The method could be detected KV from DNA clone at 1×102copies/㎕, also could be applied not only cDNA but also field samples. Especially, this method was developed direct detection by the naked eye using SYBR Green Ⅰ and Gene-Finder™ Nucleic acid fluorescent dye. In conclusion, KV-LAMP may be a useful method for the detection Kakugo Virus from the field. Kakugo Virus(KV)는 외관상으로는 건강하지만 공격적인 성향을 나타내는 일벌의 뇌에서 분리된 picorna virus 계열의 것이다. LAMP는 thermocycler 같은 고가의 장비없이도 유전 자를 증폭할 수 있는 등온증폭방법으로, 본 연구에서는 쉽고 빠르게 KV를 진단할 수 있는 KV-LAMP법을 개발하였다. KV의 특이적인 염기서열(GenBank, AB250362.1)에 근거한 KV-F3 / B3 / FIP / BIP, 4개의 primer를 사용하여 59℃의 등온조건하에서 60분간 정치하여 DNA가 성공적으로 증폭됨을 확인하였다. 1×102copies/㎕까지 증폭이 가능하며 실제 꿀벌에서도 검출가능함이 확인되었고, SYBR GreenⅠ과 Gene-Finder™ Nucleic acid fluorescent dye를 사용하여 전기영동 없이 육안으로 관찰할 수 있었다. 따라서 KV-LAMP 법을 통하여 KV를 신속하고 정확하게 검출함으로써 현장에서 유용하게 적용될 수 있을 것 으로 사료된다.

일부 도시지역 인문고교생의 체격과 영양상태에 관한 연구

윤태영,최중명,박순영,유동준,김형석,구도서,박양원 慶熙大學校 醫科大學 1986 慶熙 醫大 論文集 Vol.11 No.1

중량물 운반시 잡기유형과 부하단계에 따른 생리학적 연구

윤양진,이훈식,윤길환,정미라,이재규,이중숙,서국웅 釜山大學校 附設 體育科學硏究所 1999 體育科學硏究所 論文集 Vol.15 No.-

The purpose of this study was to examine physiological changes of the body according to various weight loads and the holding during carrying object. The experiments were conducted with utilizing physiological analyses for five male and five female adults as subjects. The results were as followed. 1. Any significant difference was not shown in heart rate, ventilation and amount of oxygen uptake of male subjects in the case of no load and 5kg. And also no significant difference was shown in expiratory exchange ratio of male subjects of no load, 5kg and 10kg, but in other measure items and other stages of weight was shown. 2. Significant differences were shown in heart rate, ventilation, defluxion of carbon dioxide, oxygen uptake per body weights, respiratory exchange rate, respiration rate and all other measure items of female subjects in all stages of load. 3. In the MMH job, there was no influence on male subjects up to 5kg loads, but there was an significant difference in male subjects in load walking from 5kg. It shows that even in 5kg weight MMH work, female subject's expiratory function was influenced. 4. 10kg load was the limit weight of female subjects and 15kg was of male subjects when they walked on treadmills with 4km/h velocity for 3 minutes, holding loads in the right and vertical types.

고양이 감각 및 운동겉질에서 calbindin D-28k와 parvalbumin에 대한 면역세포화학적연구

김종중,장인엽,정윤영,윤상필,문정석 대한해부학회 1998 Anatomy & Cell Biology Vol.31 No.2

우리나라 小兒 死亡原因의 特性 變遷에 關한 硏究

尹重洙,李時伯 서울大學校保健大學院 1995 國民保健硏究所硏究論叢 Vol.5 No.2

Recently, the National Health Insurance Scheme has been improved. The fertility rate has been slowed down while life expectancy is constantly increasing. It is significant to study on children mortality which is improtant for the development of the national and provied the basis of policy establishment. This study is intended to analyze the causes of children mortality on the basis of Mortality Report Data from 1982 through 1993. The major findings of the study are as follows: Under one year old group: The congenital abnormality and perinatal diseases increase whild infection and parasistic diseases, respiratory diseases, cardiovascular disease, digestive diseases and unknown symptoms and diagnosis decreases. Age group of 1-4: The congenital abnormality, injury and poisoning and neoplasm increases and the decreasing trend is similar to that of under one year old group. Age group of 5-14: increasing and decreasing trend are similar to those of 1-4 age group. The neoplasm of 0-4 and 5-14 age group is increasing faster than other diseases. The ten major death causes of children are found to be follows: 1) accidents, 2) congenital abnormality, 3) neoplasm, 4) pneumonia & bronchitis, 5) perinatal diseases, 6) cerebral palsy and paralysistic diseases, 7) heart diseases, 8) meningitis, 9) homicide, and 10) cerobrovascular diseases.