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PCR-제한효소절편길이다형성법을 이용한 RHD 1227G>A 및 1222T>C 검출
김우신,박건 대한수혈학회 2021 大韓輸血學會誌 Vol.32 No.1
Background: DEL is an RhD variant that cannot be detected by routine serologic tests because of the extremely low expression of the RhD antigen. Detecting the common genotypes of RHD 1227G>A and 1222T>C in Korean DEL is important for safe and efficient blood transfusions. Therefore, in this study, a PCR-restriction enzyme fragment polymorphism (RFLP) method was applied to detect RHD 1227G>A and 1222T>C. Methods: DNA extracted from the blood of each segment of 56 units of RhD-negative red blood cell were used. The promoter, exon 7 and exon 9 of RHD, and exon 9 of RHCE were amplified. The PCR products of RHD exon 7, RHD exon 9, and RHCE exon 9 were treated with the restriction enzymes HpyAV and MspI, and the RFLP patterns were observed by electrophoresis. The results of PCR-RFLP of RHD exon 9 were confirmed by PCR-direct sequencing. Results: RHCE exon 9 was amplified in all 56 DNAs. RHD promoters, exon 7, and exon 9 were all amplified in 10 samples, RHD promoter, exon 7, and exon 9 were not amplified in 38 samples, and RHD promoter only was amplified in eight samples. As a result of the RHD exon 9 PCR-RFLP performed on 10 samples with all targets amplified, 10 samples were determined to be 9 samples with 1227G>A and 1 sample with 1222T>C. The PCR-RFLP result and the sequencing result were 100% identical. Conclusion: PCR-RFLP using HpyAV and MspI is a reliable and applicable method for detecting RHD 1227G>A and 1222T>C in serologically RhD negative samples. (Korean J Blood Transfus 2021;32:28-34)
김우신,강석형,이신아,류민선,박성훈 대한심장학회 2011 Korean Circulation Journal Vol.41 No.8
Paravalvular abscess is a serious complication of infective endocarditis. The aortic valve and its adjacent ring are more susceptible to abscess formation and paravalvular extension than the mitral valve. A 15-years old patient with bicuspid aortic valve presented with staphylococcal tricuspid valve endocarditis complicated by para-aortic abscess that ruptured into the aortic sinus. We report the clinical, laboratory and echocardiographic features and treatment of this patient and conduct a literature review on this subject.
김우신,박성훈 한국심초음파학회 2011 Journal of Cardiovascular Imaging (J Cardiovasc Im Vol.19 No.1
Background: Atrial fibrillation (AF) is the most common cardiac arrhythmia with a population prevalence of about 1%. Natriuretic peptide level is elevated in patients with AF with diastolic dysfunction even with a normal left ventricular (LV) ejection fraction. The N-terminal pro-brain natriuretic peptide (NT-proBNP) level and Doppler echocardiographic parameters for diastolic function have shown correlation with LV filling pressures. We aimed to evaluate the relationship between echocardiographic parameters and serum NT-proBNP in patients with AF with preserved LV ejection fraction. Methods: We examined transthoracic echocardiography and NT-proBNP levels in the patients with AF and patients with sinus rhythm. Blood samples were taken for serum NT-proBNP measurements within 24 hours of echocardiographic examination. The group 1 was the patients with sinus rhythm (n = 30, mean age 68 ± 13 years) and the group 2 was the patients with AF (n = 33, mean age 70 ± 14 years). Results: The group 2 patients had significantly higher mitral E, E’ (lateral annulus), E/E’ (septal annulus), left atrial (LA) volume index, LA size, pulmonary vein diastolic velocity, and NT-proBNP level than those of group 1 patients (p < 0.05). The area under the receiver-operating characteristic curve showed a NT-proBNP had good diagnostic power for E/E’ (septal annulus) > 15 in patients with AF at cutoff value of 433 pg/mL. Conclusion: NT-proBNP level is well correlated with Doppler echocardiographic parameters of diastolic function in patients with AF and preserved LV ejection fraction. NT-proBNP level more than 433 pg/mL may suggest elevated LV filling pressure in patients with AF. Background: Atrial fibrillation (AF) is the most common cardiac arrhythmia with a population prevalence of about 1%. Natriuretic peptide level is elevated in patients with AF with diastolic dysfunction even with a normal left ventricular (LV) ejection fraction. The N-terminal pro-brain natriuretic peptide (NT-proBNP) level and Doppler echocardiographic parameters for diastolic function have shown correlation with LV filling pressures. We aimed to evaluate the relationship between echocardiographic parameters and serum NT-proBNP in patients with AF with preserved LV ejection fraction. Methods: We examined transthoracic echocardiography and NT-proBNP levels in the patients with AF and patients with sinus rhythm. Blood samples were taken for serum NT-proBNP measurements within 24 hours of echocardiographic examination. The group 1 was the patients with sinus rhythm (n = 30, mean age 68 ± 13 years) and the group 2 was the patients with AF (n = 33, mean age 70 ± 14 years). Results: The group 2 patients had significantly higher mitral E, E’ (lateral annulus), E/E’ (septal annulus), left atrial (LA) volume index, LA size, pulmonary vein diastolic velocity, and NT-proBNP level than those of group 1 patients (p < 0.05). The area under the receiver-operating characteristic curve showed a NT-proBNP had good diagnostic power for E/E’ (septal annulus) > 15 in patients with AF at cutoff value of 433 pg/mL. Conclusion: NT-proBNP level is well correlated with Doppler echocardiographic parameters of diastolic function in patients with AF and preserved LV ejection fraction. NT-proBNP level more than 433 pg/mL may suggest elevated LV filling pressure in patients with AF.
김우신,편욱범,강병철 대한심장학회 2011 Korean Circulation Journal Vol.41 No.9
Background and Objectives: Dysfunction of arteriovenous fistulas (AVFs) and arteriovenous grafts (AVGs) contributes significantly to morbidity and hospitalization in the dialysis population. We evaluated the primary patency of AVFs following percutaneous transluminal angioplasty (PTA) in haemodialysis patients. Subjects and Methods: We performed 231 interventions in 118 patients with a mean age of 62.1±12.9 years. We performed 122 interventions in 53 AVG patients (44.9%), and 109 interventions in 65 AVF patients (55.1%). If there was thrombosis of the vascular access, urokinase was administered and/ or thrombus aspiration was performed. The stent was inserted when balloon dilatation did not expand sufficiently or elastic recoil occurred. Results: For the 118 patients, the median patency time was 10.45±10.29 months at 92 months of follow-up. The primary patencies for stenotic AVFs at 6, 12, 24, 36, 48, and 60 months were 63.4%, 41.4%, 17.0%, 9.7%, 7.3%, and 2.4%, respectively. The primary patencies for AVGs at 6, 12, 24, and 36 months were 36.9%, 19.5%, 10.8%, 2.1%, respectively, and were obtained by means of the Kaplan-Meier analysis (log rank=6.42, p<0.05). The median patency time was 11.0 months and 4.45 months in the non-thrombus and thrombus groups, respectively. The complication rate was 1.73% (4/231); two cases of pseudoaneurysms and two cases of extravasation were detected. All therapy failures (5/231) occurred in thrombotic lesions of AVGs and were treated surgically. Conclusion: PTA is an efficacious method for the correction of stenosis of AVFs for hemodialysis, thus prolonging the patency of the fistulas.
관골에 발생한 양성연골아종 (chondroblastoma)의 임상치험례
김한중,윤진호,이동진,김우신 大韓成形外科學會 1996 Archives of Plastic Surgery Vol.23 No.4
Chondroblastoma represents approximately 1% of all the primary bone tumors. Most of these tumors arise in the epiphysis of long bones. Chondroblastoma of the cranial bone and facial bone is extremely rare. Furthermore, chondroblastoma involving the zygoma of the facial bone has not been reported in the literatures. Treatment of the chondroblastoma in the skull and facial bone has been the same as treatment usually used in the long bones includes simple curettate and radiation therapy. But chondroblastoma of the skull and facial bone is a benign but locally aggressive tumor. On the basis of our case and review of the literature, we recommend complete radical surgical excision as the primary treatment for chondroblastoma of the zygomatic bone. We report the cast of 21-year-old man with benign chondroblastoma in the zygomatic bone. The clinical features, radiologic and histologic appearances, and treatments are also discussed.
김한중,김우신,이동진,윤진호 大韓成形外科學會 1996 Archives of Plastic Surgery Vol.23 No.6
From July 1988 to July 1995, 12 Patients underwent surgery for recurred blepharoptosis with frontalis muscle transfer. Their previous operations of blepharoptosis were frontalis muscle suspension in 9 cases, creation of supratarsal folds in 1 case, levator resection in 1 case, and frontalis muscle transfer in 1case. 6 patients were female and 6 were male. 4 patients had bilateral and 8 patients had unilateral blepharoptosis. Patients follow-up period ranged from 6 months to 24 months. The average operation interval between first operation to re-operation was 13.5 months. The results were as follows. : 1. 11 patients (91.6%) had gained the levator excursion of over 7mm and reduced the hight difference of both palpebral fissures less than 2mm after the reoperation (frontalis muscle transfer). The overall results were more than satisfactory. 2. We used a single incision instead of double resulting in one less visible scar. Complications such as hematoma and forehead hypoesthesia were also reduced by using short U-shaped frontalis muscle flap. 3. The asymmetric double folds, eyelid notching, lagophthalmus and abnormal eyebrow position occurring after the frontalis muscle suspension can be corrected by the frontalis muscle transfer. From these results the frontalis muscle transfer is judged to be a good secondary operation for patients with complicated and recurred biephaloptosis.