Inhibition of oxidative stress induced‑cytotoxicity by coptisine in V79‑4 Chinese hamster lung fibroblasts through the induction of Nrf‑2 mediated HO‑1 expression

Hyeon‑Gyun Jo,Cheol Park,이혜숙,Gi‑Young Kim,Young‑Sam Keum,Jin Won Hyun,Taeg Kyu Kwon,Yung Hyun Choi,Su Hyun Hong 한국유전학회 2021 Genes & Genomics Vol.43 No.1

Background Coptisine is a natural alkaloid compound and is known to have multiple benefcial efects including antioxidant activity. However, whether it can protect lung fbroblasts from oxidative damage has not been studied yet. Objectives To investigate the potential inhibitory efect of coptisine against oxidative stress in V79-4 lung fbroblast cells. Methods V79-4 cells were treated with H2O2 (1 mM) in the presence or absence of coptisine (50 µg/ml), N-acetyl cysteine (NAC, 10 mM) or zinc protoporphyrin IX (ZnPP, 10 µM) for the indicated times. The alleviating efects of coptisine on cytotoxicity, cell cycle arrest, apoptosis, reactive oxygen species (ROS) production, DNA damage, mitochondrial dynamics, and inhibition of ATP production against H2O2 were investigated. Western blot analysis was used to analyze the expression levels of specifc proteins. Results Coptisine inhibited H2O2-induced cytotoxicity and DNA damage by blocking abnormal ROS generation. H2O2 treatment caused cell cycle arrest at the G2/M phase accompanied by increased expression of cyclin-dependent kinase (Cdk) inhibitor p21WAF1/CIP1 and decreased expression of cyclin B1 and cyclin A. However, these efects were attenuated in the presence of coptisine or NAC. Coptisine also prevented apoptosis by decreasing the rate of Bax/Bcl-2 expression in H2O2-stimulated cells and suppressing the loss of mitochondrial membrane potential and the cytosolic release of cytochrome c. In addition, the activation of nuclear factor-erythroid-2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) was markedly promoted by coptisine in the presence of H2O2. However, zinc protoporphyrin IX, a potent inhibitor of HO-1, attenuated the ROS scavenging and anti-apoptotic efects of coptisine. Conclusions Based on current data, we suggest that coptisine can be used as a potential treatment for oxidative stress-related lung disease.

한국 근대문학 연구의 새 지평 ; 근대시 연구의 새 지평 ―전통주의적 연구를 중심으로-

박현수 ( Hyeon Su Park ) 현대문학이론학회 2008 現代文學理論硏究 Vol.0 No.33

The purpose of this paper is to investigate the notion and present state of traditionalist research in the study of Korean poetry. In this paper, traditionalist research means a study which to search out new notions and paradigms of discourse in Korean traditional thought and to apply to the study of modern Korean poetry by creative interpretation of those. Closely looking into traditionalist studies, there are five categories; general conceptual, Buddhist, Taoist, neo-Confucianist, Korean fundamental philosophic study. General conceptual study, based on traditional philosophic concepts in the history of Korean thought, was conducted by Yun Jae-geun, Kim Young-seok and Choi Seung-ho. Buddhist study, by Song Uk, Taoist study, by Lee Seonghee and Kim Kyoung-bog, neo-Confucianist study, by Jo Dong-il and Park Hyeon-su, and Korean fundamental philosophic study, by Seo Jeong-ju, Shin Beom-sun and Kim Ji-ha. Though these studies, in the making process, had some blurs in logical refinement, they have very important significance in Korean literary study in that, they tried to find a new and creative theory. It is also worthy to note that traditionalist studies brought a change in the study of Korean literature subordinated every discourse of Korean literature to the western theory.

Fabrication and evaluation of SU-8 based wireless pressure sensor

Gang-Hyeon Sun(선강현),Dong-Su Kim(김동수),Dong-weon Lee(이동원) 대한기계학회 2021 대한기계학회 춘추학술대회 Vol.2021 No.6

In the recent years, the development of wireless sensors has attracted attention, with the development of MEMS(Micro-Electro-Mechanical-System) technology. The pressure sensor, one of the wireless sensor, is a device that measures the applied force, pressure, by a liquid or gas on a surface and translates it to the electrical signal. We fabricate this sensor based on capacitive type which measures change in capacitance with two metal plates. One of the two capacitor plates acts as the diaphragm that is displaced by the pressure, changing the capacitance of the circuit. Variation of signal detected by network analyzer evaluates the resonance frequency and phase shift with different interrogate distance and pressure force. [그림 본문 참조]

윤동주 「서시」의 의미 연구

김현수(Kim Hyeon-su) 한국시학회 2011 한국시학연구 Vol.- No.31

Yun, Dong-ju’s 「The First Poem」 is producing a variety of meanings by omitting its meanings and expressing it indirectly. This paper is to understand the meaning of the work, not the intention of the poet, based on the fact that literature is content to exist by itself. The meaning of a work is the real meaning which the work represents through linguistic combination and structure. This poem is divided into two stanzas. The first stanza of 1~8 lines is expressing the poet’s feelings, and the second stanza of 9 lines shows the imaginary scenes of nature. The first stanza is divided into 1~4 lines containing mental distress and 5~8 lines representing love and practice will. The scenes of the second stanza are symbolically representing the heart of the poet who has overcome conflicts and troubles. 「The First Poem」 is a work which has encapsulated the pure heart seeking beautiful and peaceful world with concise and implicative words. In terms of space, this poem is divided into Heaven and Earth, and in terms of conflicts, it shows the process of division and combination of the realistic ego which is tormented in the real world and the essential ego which wishes for the beautiful and peaceful heavenly world. Though the realistic ego is ambivalent and worried about the gap between reality and ideals, it recovers its essential ego through aiming for the heavenly world and pursues the peaceful and pure world which it always longs for inwardly.

고포도당이 백서 사구체 메산지움 배양세포에서 p38 MAPK 활성화 및 Fibronectin 생성에 미치는 영향

유태현 ( Yu Tae Hyeon ),허종호 ( Heo Jong Ho ),류동열 ( Lyu Dong Yeol ),김현욱 ( Kim Hyeon Ug ),이수현 ( Lee Su Hyeon ),김진주 ( Kim Jin Ju ),정동섭 ( Jeong Dong Seob ),최규헌 ( Choe Gyu Heon ),이호영 ( Lee Ho Yeong ),한대석 ( Han 대한신장학회 2003 Kidney Research and Clinical Practice Vol.22 No.5

목 적 : 고포도당으로 자극한 백서 메산지움 배양세포에서 p38 mitogen activated protein kinase (MAPK)의 활성화와 p38 MAPK의 상부와 하부인자로 알려져 있는 MAPK kinase 3/6 (MKK3/6)와 c-AMP responsive element binding protein (CREB)의 활성화, 그리고 fibronectin 합성을 자극 시간에 따라 관찰하고, p38 MAPK 경로와 fibronectin 합성 사이의 연관성을 규명함으로서 당뇨병성 신증의 병태생리를 규명하고자 하였다. 방 법 : 백서 메산지움 배양세포를 정상 포도당 (5.6 mM), 고포도당 (30 mM), 또는 정상 포도당+만니톨 (24.4 mM)로 자극한 후 첫째 p38 MAPK; 둘째 p38 MAPK의 상부 인자인 MKK3/6; 셋째 p38 MAPK의 하부인자인 CREB; 넷째 MAPK phosphatase-1 (MKP-1)의 변화를 자극 시간 (3분-48시간)에 따라 Western blot을 이용하여 관찰하였으며, 다섯째 fibronectin의 변화는 RT-PCR과 ELISA를 이용하여 확인하였다. 결 과 : 고포도당으로 자극한 백서 사구체 메산지움 배양세포에서 p38 MAPK 경로가 활성화 되었다. Total p38 MAPK 단백은 변화가 없었으나, 활성화된 phospho-p38 MAPK 단백은 고포도당군에서 자극 10분 후부터 정상 포도당군에 비해 의의있게 증가되었으며 (평균 1.9배), phospho-CREB 단백 역시 고포도당군에서 자극 10분 후부터 의미있게 증가되었다 (평균 2.5배). 이에 비해 p38 MAPK의 상부인자인 phospho-MKK3/6는 고포도당군에서 자극 3분 후부터 의의있게 증가되었다 (평균 2.4배). 또한, MKP-1 단백 발현은 고포도당군에서 p38 MAPK의 활성이 증가하기 시작한 시기와 동일한 시기 (자극 10분 후)부터 증가하기 시작하였다 (평균 1.9배). Fibronectin mRNA와 세포 배양액내 fibronectin은 고포도당군에서 자극 48시간 후에 각각 1.7배와 1.5배 증가되었으며, 이러한 증가는 p38 MAPK 억제제인 SB203580 (1 μM)에 의해 각각 73%와 69% 억제되었다. 결 론 : 고포도당으로 자극한 백서 사구체 메산지움 배양세포에서 p38 MAPK 경로의 활성화를 확인하였으며, p38 MAPK 억제제가 고포도당에 의한 fibronectin의 합성 증가를 억제시키는 것으로 보아 당뇨병성 신증에서 관찰되어지는 fibronectin의 축적에 p38 MAPK 경로가 관여할 것으로 생각된다. Background : The p38 mitogen-activated protein kinase (MAPK) pathway is activated by several stress factors, potentially leading to cellular apoptosis and growth. Little is known about the pattern of p38 MAPK pathway activation in mesangial cells under high glucose conditions. We examined the activity and expression of p38 MAPK members, 1x78 MAPK, MAPK kinase 3/6 (MKK3/6), c-AMP responsive element binding protein (CREE), and MAPK phosphatase-l (MKP-1) in cultured rnesangial cells exposed to high glucose. Methods : Mesangial cells were subcultured from rat glomeruli isolated by sieving technique. After serum restriction for 48 hours rnesangial cells were exposed to 5.6 mM glucose (low glucose, LG), 5.6 mM glucose+24.4 mM mannitol (LG+M), or 30 mM glucose (high glucose, HG) for 3 minutes to 48 hours with or without SU203580. Western blot was performed to determine the activity and protein expression of p38 MAPK members. R`I-I`CR and ELISA were performed for fibronectin mRNA expression and fibronectin synthesis, respectively. Results : p38 MAPK and CREB activities were significantly increased in rnesangial cells exposed to FIG compared with LG or LG+M after 10 minutes and was sustained at higher levels to 48 hours (p<0.05), but total p38 MAPK and CREB protein expressions did not differ. MKP--1 showed a similar pattern as p 3 MAPK and CREE (p<O.O.i). MKK3/6 acitvity was significantly higher in HG cells after 3 minutes and remained at higher levels throught the study period (p<0.05). Fibronectin mRNA expression and fibronectin synthesis were significantly increased in mesangial cells exposed to HG after 48 hours (p<0.05). SB203580 (1 pM) pretreatment for 1 hour significantly reduced HG-induced fibronectin mRNA expression and fibronectin synthesis by 73% and 69%, respectively (p<0.05). Conclusion : p38 MAPK activity was increased in mesangial cells exposed to HG in parallel with increased MKK3/6 activity, resulting in CREB activation and increased fibronectin synthesis. This activated p38 MAPK may play a role in the pathogenesis of diabetic nephropathy.

산업단지 및 일반생활 지역의 공기 중 톨루엔에 대한 개인노출 및 생체시료의 상관성 분석

이병준(Byoungjun Lee),허 정(Jung Heo),정다영(Dayoung Jung),김순신(Sunshin Kim),류현수(Hyeon-Su Ryu),최민지(Min-Ji Choi),솨이지엔페이(Jian-Fei Shuai),임성국(Sung-Guk Im),양원호(Wonho Yang) 한국환경보건학회 2017 한국환경보건학회지 Vol.43 No.4

Objectives: The purpose of this study was to assess the correlation relationship between personal exposure and urinary hippuric acid in biological monitoring for airborne toluene in an industrial complex and in general environments. Methods: Personal exposure to toluene and its metabolite, hippuric acid, in urine were simultaneously measured in occupational environments area near an industrial complex and in general environments. The study subjects were divided into three types: 137 workers who use organic solvents in the workplace, 210 residents living near a dyeing industrial complex, and 379 residents living in general environments. The toluene exposures of workers and residents were measured by a passive sampler for four days. The urine of participants was sampled when the passive samplers of personal exposure were collected. Results: The toluene and hippuric acid concentrations of workers were the highest, followed by the concentrations of residents living near a dyeing industrial complex and residents living in general environments. The coefficient of correlation between the concentrations of toluene and hippuric acid among workers was 0.749 (p<0.01) in the workplace. On the contrary, correlations between the concentrations of toluene and hippuric acid among residents living near a dyeing industrial complex and residents living in general environments were all not significant. The relationship between the concentrations of hippuric acid and toluene in three types could be described by the exponential growth model. Conclusions: This study analyzed the relationships between toluene exposure and the concentrations of hippuric acid in urine in high, middle, and low exposure environments, and could be described by the exponential growth model.

진행성 폐 종양 환자에서 종양 내 OK-432 주입술의 효과

정수현,김성빈,박진희,탁희상,옥철호,장태원,정만홍,천봉권 고신대학교 의과대학 2009 고신대학교 의과대학 학술지 Vol.24 No.2

Background: Intratumoral injection of OK-432 produced significant antitumor effects. There are reports that OK-432 was injected directly into tumor tissue on gastrointestinal tracts and HCC tissue, producing decrease in serum alpha-fetoprotein level. We adopted this technique for treating locally advanced lung cancer. The object of this study is to evaluate the local therapeutic efficacy, side effects of injection for treating symptomatic mass. Methods: Twenty lung cancer patients (13 males and 7 females) underwent sonography guided OK-432 injection. We analyzed relationships between response of OK-432 and response of skin test, histology and age. Pain that patients feel was evaluated by VAS (Visual Analog scale). Results: A significant decrease of tumor size was observed. The mean pre-injection diameter of the lesions was 54.7±11.3mm, PR rate were 15.0% (3/20) and minimal change (50%<tumor size decreased.) patients were 35.0% (7/20). The VAS score before injecting OK-432 into the lesion was 5±3, this was decreased to 2.5±1.5 (p=0.04). Object response was significantly correlated with skin response, adenocarcinoma, and age. Conclusion: A significant decrease of tumor size and pain was observed. So OK-432 is a valuable treatment option for patients with symptomatic lesion from advanced lung cancer who are not amenable to further therapy.

RT-LAMP를 이용한 콩황화일반모자이크바이러스의 진단

배대현, 이영훈, 김봉섭, 윤영남, 강범규, 최만수, 구성철, 김현태, 윤홍태, 이수헌, 백인열 忠北大學校 農業科學硏究所 2014 農業科學硏究 Vol.30 No.2

Soybean yellow common mosaic virus (SYCMV) has been recently reported, it has been occurred a lot with Soybean mosaic virus (SMV) and Soybean yellow mottle mosaic virus (SYMMV) in soybean field. SYCMV belongs to genus of Sobemovirus and induced viral symptoms with yellowing, mottle and mosaic. A reverse transcription loop-mediated isothermal amplification (RT-LAMP) method allowed one-step detection of gene amplification by simple procedure and needed only a simple incubator for isothermal template. This RT-LAMP method allowed direct detection of RNA from virus-infected plants without thermal cycling and gel electrophoresis. In this study, we designed RT-LAMP primers named SYCML-F3/B3/FIP/BIP from coat protein gene sequence of SYCMV. After the reaction of RTLAMP, products were identified by electrophoresis and with the detective fluorescent dye, SYBR Green I. under daylight and UV light. Optimal reaction condition was at 63 for 60min and the primers of RTLAMP showed the specificity for only SYCMV tested in this study.

의미 분석 단계에서의 정보 흐름 제어

조현욱,김명수,정은환,유재휘,윤병주 명지대학교 공학기술연구소 1986 공학기술연구소 논문집 Vol.1 No.-

One of protection methods for computer internal data is to pursue data flow in program and to control its flow. In this thesis, we propose a mechanism which guarantees secure information flow in a program. By investigating security classes of all the variables in a statement and judging necessary condition for secure information flow, proposed model descriminates the program whether it is secure or not. Out model can be implemnted at language translation time, particularly semantic analysis phase, by pursuing security class transfer of each statement.

극장 관람료의 합리적 분배에 관한 연구

이현승,맹수현 영상예술학회 2009 영상예술연구 Vol.15 No.-

부율이란 부금비율의 약칭으로 극장에서 거두어들인 입장수입에서 세금을 제한 후, 배급사와 극장 측이 약정에 따라 수입을 분배하는 비율을 의미한다. 국내에서는 통상 서울 지역의 경우 한국영화는 5:5, 외국영화의 경우 6:4(배급사:극장)로 배분하고 있으며, 지방의 경우 한국 영화와 외국영화의 구분없이 5:5의 배분을 하고 있다. 이는 1980년대 후반 할리우드 영화의 직접배급이 시작되면서 할리우드 영화에 비해 한국 영화의 경쟁력이 취약하여 생긴 관행이다. 하지만 한국 영화의 점유율이 15~30%를 유지했던 8·90년대와는 달리, 평균 50~60%의 점유율을 기록하고 있는 2000년대 이후까지 과거의 관행이 유지되고 있다는 것에는 문제가 있다. 또한 2002년부터 한국 영화의 투자 수익률이 하락하고, 투자, 제작, 배급, 홍보 및 마케팅 등 1차 상영관련 시장의 수익률이 적게는 10%에서 많게는 60% 이상의 마이너스 행진을 기록하고 있는 상황 속에서, 극장 상영부분만이 유일하게 이익을 보고 있다는 것은 산업내 왜곡된 수익구조가 존재한다는 의미이기도 하다. 미국이나 일본, 프랑스에서 극장의 관람료 수익 분배 비율은 10~50% 정도이다. 이를 한국 부율 관행과 비교한다면 그리 나쁘지 않은 수익 분배라고 생각할 수 있으나 사실은 그렇지 않다. 세계적으로 윈도우별 영화시장의 규모 중 극장 매출은 31%이다. 즉 극장 이외의 다른 수익 창출 구조가 안정적으로 유지되고 있다는 뜻이다. 하지만 한국의 경우 부가판권 시장의 몰락으로 투자자에게 돌아가는 수익 중 극장 매출이 차지하는 비율은 전체 매출의 약 80%이다. 그렇기 때문에 극장 관람 수입의 합리적인 배분은 그 어느 나라보다 절실한 실정이다. 영화 산업 내 각 분야의 주체들에게 합리적으로 수익이 분배되기 위해서는 다음의 네 가지 사안들이 고려되어야 한다. 첫째, 제작비, 작품의 가치, 흥행 예상 기대치, 마케팅 규모 등을 두로 고려하여 각각의 작품들은 개별적인 부율 책정에 들어가야 한다. 둘째, 미국의 슬라이딩 시스템이나 프랑스의 ‘월요일 협상(au lundi)’, ‘극장의 ‘통로’(Les ‘couloirs’ de salles)’ 방식과 같이 기간에 따라 차별적인 부율을 적용해야 할 것이다. 셋째, 한국 영화산업의 특수성을 고려한 부율 조정이 필요하다. 넷째, 공정한 거래 절차를 유지하여 바람직한 계약 관행을 확립해야 할 것이다. 한국 영화 산업 내에서 부율 책정은 그동안의 오랜 관행으로 각각의 이익집단간의 이해가 상충하여 쉽게 개선이 될 수 있는 문제는 아닐 것이다. 그러나 이는 한국 영화의 투자·제작 기반을 튼튼히 하여 극장에서도 지속적으로 양질의 콘텐츠를 수급할 수 있도록 하기 위함이며, 나아가 각 사업 분야 모두 합리적인 이익 창출을 통한 안정적인 수익 구조를 실현하여 영화 산업 전반의 성장을 구현하기 위함이다. 또한 합리적 부율 책정을 통해 한국 영화계의 과제인 다양성 영화의 안정적 제작·배급의 풍토 역시 마련될 수 있을 것임을 기대해본다. Box-office split stands for the rate of distribution of box-office earnings between a distributor and a movie theater according to an agreement between them after deducing tax from the earnings. In Seoul, box-office split is usually 5:5 (distributor:movie theater) for domestic movies and 6:4 for foreign movies. In domestic local areas, it is 5:5 in general, regardless of the origins of movies. The difference of box-office split between domestic and overseas movies has been derived from the lower competitiveness of domestic movies than Hollywood movies since Hollywood movies were directly distributed in the late days of the 1980s. The market share of domestic movies was only 15~30% in the 1980s and 1990s, but it has changed to 50~60% since the 2000s. In this vein, still maintaining the past practice is problematic. Besides, since 2002, the investment returns of domestic movies have decreased gradually and the earning rates of primary screening-related market activities, such as investment, production, distribution, publicity and marketing, has been reduced in the rate of minimal 10% to maximal 60%. However, there still exist profits in the part of movie theaters. It means that there is a distorted earning structure in the domestic film industry. The rate of distribution of box-office earnings in the United States, Japan and France is 10~50%. Comparing to the domestic rate, it seems to be not bad. However, it is not the case in reality. In the world, the sales amount of movie theaters occupies only 31% of total earnings which come from all the sales windows of movie market. This means that in overseas film industry there are stable earning structures other than movie theaters. However, with the destruction of domestic additional copyright market, the sales amount of movie theaters occupies 80% of investors' total income in the domestic film industry. Therefore, it is urgent to rationally distribute the box-office earnings of domestic movie theaters. In order for the box-office earnings of domestic movie theaters to be rationally distributed among all the parties of film industry, following four alternatives must be taken into account:First, each movie must have its own box-office split considering production cost, the value of movie, expected box-office value, marketing scale and so on;second, like American sliding system or French ‘au lundi’ and ‘Les couloirs de salles’ systems, differentiated distribution rates must be applied on the basis of the period; third, we need an adjustment considering Korean films industry's speciality; and fourth, fair transaction procedures and proper contract practices must be established. Since the existing box-office split is a practice of long standing and the interest of every party is contrary, the establishment and improvement of rational box-office split in domestic film industry seems to be not easy. However, to establish a rational box-office split is necessary for strengthening the investment and production base of domestic movies and supplying quality movies to movie theaters. In addition, it is necessary to establish a rational investment return-based stable earning structure for all the related fields of film industry and thus realize the growth of the film industry in general. The investigator hopes that with the establishment of rational distribution rate, the environment of stable production and distribution of diverse movies, a task of long standing in the domestic film industry, will be created in the future.