심근경색 모델 흰쥐에서 스트레스가 B-type Natriuretic Peptide 발현 및 심박변이율 변화에 미치는 영향

이삼윤(Sam Youn Lee),이미경(Mi Kyoung Lee),김남호(Nam-ho Kim),주민철(Min Cheol Joo),조항정(Hyang Jeong Jo),강지숙(Ji Sook Kang),김병숙(Byung Sook Kim),윤선식(Sun Sik Yoon),최을식(Eul Sig Choi),이문영(Moon Young Lee) 대한스트레스학회 2010 스트레스硏究 Vol.18 No.3

심박변이율의 감소는 심근 경색 환자에서 예후의 악화와 관련되어 있다. 심박변이율 중 very low frequency가 심부전환자의 예후를 판단할 수 있는 독립적 인자로서 사용이 가능하다고 보고된 바 있으며, 심실의 압력 증가에 반응해서 생성되는 B-type natriuretic peptide가 심근 경색의 예후 인자로서 사용할 수 있음 역시 보고된 바 있다. 본 연구에서는 심근경색 모델을 제작하여 일정의 회복 기간을 거친 다음 다시 일정 기간의 스트레스를 겪게 한 후 심박변이율의 변화 및 심실 조직에서의 BNP 발현 정도를 비교하여 스트레스에 의한 영향을 관찰하고자 하였다. Sprague-Dawley계 수컷 흰쥐 15마리를 대상으로 하였다. 실험동물은 인위적 스트레스 및 수술을 받지 않은 대조군(CON, n=5), 심근경색 수술을 받은 후 restraint stress를 받지 않은 군(MI+No Stress, n=5), 심근경색 수술을 받은 후 1주일 동안 restraint stress를 받은 군(MI+Stress, n=5)으로 분류하였다. 심근경색 모델 제작 직후 15분 이상 심전도의 변화를 기록하였으며, 2개월 정도 후 심근경색 수술을 받은 동물을 두 군으로 나누어 그 중 한 군에는 1주일 동안 restraint stress를 가한 후 restraint stress를 가하지 않은 동물과 심박변이율을 비교 분석하였다. 심실 조직은 좌심실 전벽 부위의 위축을 관찰할 수 있었고, 대식세포에 의하여 응고, 괴사된 심근세포의 탐식과 혈관이 풍부한 육아조직 및 섬유 변화를 볼 수 있었다. 심박변이율은 심근경색 수술을 받은 직후 심박수는 유의한 증가를 보였고, standard deviation of the normal to normal intervals (SDNN), very low frequency (VLF) 및 low frequency (LF)의 유의한 감소를 보였다. 심근경색 수술을 받았던 동물에서 두 달 정도의 회복 기간을 거친 후 스트레스를 받지 않은 군은 심박수 및 기타 심박변이율 분석에서 SDNN 값을 제외하고는 정상군과 유의한 차이를 관찰할 수 없었던 반면 1주일 동안 스트레스를 받은 군에서는 심박수가 다시 유의하게 증가했을 뿐만 아니라 SDNN, VLF 및 LF 역시 정상군과 유의한 차이를 보였다. 이상의 결과를 종합하면 심근경색 동물에 대해 1주일 동안 스트레스를 가한 결과 심박변이율이 감소하고 심실에서의 BNP 발현은 더욱 증가하여 악화함을 보여주고 있다. Diminished heart rate variability (HRV) is associated with less favorable prognosis after myocardial infarction (MI). It has been reported that very low frequency (VLF) power in HRV analysis is an independent risk predictor in patients with congestive heart failure and B-type natriuretic peptide (BNP) can be used as a prognostic factor of MI. The purpose of this study was to investigate whether stress has an effect to the changes of BNP expression and/or heart rate variability in MI model in rats. Male Sprague-Dawley rats were divided into 3 groups: control group (CON), MI group (MI+No Stress), and MI followed by stress group (MI+Stress). MI+Stress group rats were raised for a two month recovery period after the operation, followed by being exposed to restraint stress for 2 hours per day for 1 week. Electrocardiogram was recorded after the operation and the last day after 1 week of stress. The frequency components of HRV were calculated in the frequency domain such as VLF, low frequency (LF), high frequency (HF) and so on. In HRV analysis, standard deviation of the normal to normal intervals (SDNN) was significantly reduced in both groups compared to the control group. VLF and LF also were significantly reduced in MI+Stress group compared to the control groups. In addition, BNP expression in western blotting was shown the strongest bands in MI+ Stress group among experimental groups. These results suggest that BNP and HRV were aggravated by stress in MI rat model. (Korean J Str Res 2010;18:275∼285)

Adipose sirtuin 6 drives macrophage polarization toward M2 through IL-4 production and maintains systemic insulin sensitivity in mice and humans

Song, Mi-Young,Kim, Sang Hoon,Ryoo, Ga-Hee,Kim, Mi-Kyung,Cha, Hye-Na,Park, So-Young,Hwang, Hong Pil,Yu, Hee Chul,Bae, Eun Ju,Park, Byung-Hyun Nature Publishing Group UK 2019 Experimental and molecular medicine Vol.51 No.5

<▼1><P>Adipose tissue inflammation is a reproducible feature of obesity and obesity-linked insulin resistance. Although sirtuin 6 (Sirt6) deficiency has previously been implicated in diet-induced obesity and systemic insulin resistance, the adipocyte-specific role of Sirt6 in the regulation of adipose tissue inflammation and systemic metabolic dysfunction in mice fed normal chow and in humans remains elusive. Here, using <I>Adipoq-Cre</I>-mediated adipocyte-specific Sirt6 knockout (aS6KO) mice, we explored whether adipocyte Sirt6 inhibits adipose tissue inflammation and its underlying mechanism. aS6KO mice fed normal chow gained more body weight and fat mass than wild-type mice and exhibited glucose intolerance and systemic insulin resistance. Measurement of plasma and tissue cytokines and flow cytometric analysis of adipose stromal vascular cells indicated a decrease in alternatively activated M2 macrophages in the adipose tissue of aS6KO mice. Mechanistically, Sirt6 regulated the expression of the canonical type 2 cytokine IL-4 by adipocytes in a cell autonomous manner, which in turn affects M2 macrophage polarization. Consistent with animal experimental data, the degree of obesity and insulin resistance demonstrated by the body mass index, fasting blood glucose and HbA1c correlated negatively with the expression of Sirt6 in human visceral fat tissues. Collectively, these results suggest that adipocyte Sirt6 regulates body weight gain and insulin sensitivity independent of diet, and the increased IL-4 production by Sirt6 and resultant M2 polarization of adipose tissue macrophages may attenuate proinflammatory responses in adipose tissue.</P></▼1><▼2><P><B>Obesity and diabetes: A control protein in fat</B></P><P>A protein in adipose tissue (composed of fat cells) helps protect against inflammation and the development of resistance to insulin that develops in obesity and can lead to type 2 diabetes. Researchers in South Korea, led by Eun Ju Bae at Woosuk University, Wanju, and Byung-Hyun Park at Chonbuk National University, Jeonju, investigated the role of Sirt6 in mice and in human adipose tissue. Deleting the mouse gene that codes for Sirt6 in adipocytes promoted the impaired response to insulin and associated increase in blood glucose levels that are two key aspects of diabetes. Changes in biochemical signaling pathways controlling immune cells called macrophages were implicated in these effects and suggest an anti-inflammatory role for Sirt6. Analysis of human adipose tissue supported these findings. The research will help understand how obesity promotes type 2 diabetes.</P></▼2>

韓國型 Turnip Mosaic Virus의 純化

김병훈,권오병,마병철,강미란,권무식 성균관대학교 생명과학자원연구소 1996 生命資源科學硏究 Vol.3 No.1

Chinese cabbage(Brassica campestris subsp pekinensis) useful for making a traditional Korean food, Kimchi, has been considered as the most important vegetable in Korea. In order to supply large quantity of chinese cabbage to the consumer, the mass cultivation is demanded. However, the mass production has been faced to serious problem due to fungal, bacterial and/or viral infections. It has been reported that the viral infections are the worst phatogeruc causes in a number of plants. In the chinese cabbage, turnip mosaic virus(TuMV) is the most serious pathogen. Five different strains of TuMV(Cl, C2, C3, C4, and C5) were identified in the world. We are reporting two strains(C4, C5) purified from Brassica nigra. They are all filamentous on electron micrographs. The TuMV C4 is about 1,300nm long, whereas the C5 is about 1,200nm long in average. They are larger than those reported previously(680-900nm). They will be used to produce monoclonal antibodies against the TuMV coat proteins. The antibodies arc required to develop a sensitive and specific screening methods for the TuMV infected plants.

학부모용 유아교육정책 평가척도 적용 및 타당화 : 「3-5세 연령별 누리과정」 정책을 중심으로

김병만 ( Kim Byung-man ),김미진 ( Kim Mi-jin ),김정주 ( Kim Jung-ju ) 한국아동교육학회 2019 아동교육 Vol.28 No.1

본 연구는 학부모용 유아교육정책 평가척도에 「3-5세 연령별 누리과정」 정책을 적용하여 선행적으로 개발된 평가척도의 타당도를 검토하고자 한다. 연구의 목적을 달성하기 위해 학부모 232명을 연구대상으로 선정하였고, 김병만(2018)이 개발한 유아교육정책 평가척도를 「3-5세 연령별 누리과정」 정책 중심으로 수정·보완하여 연구도구로 사용하였다. SPSS 23.0과 AMOS 8.0 program을 사용하여 평가 정도, 내용단일성, 문항변별도, 척도양호도를 확인하였고, 연구결과는 다음과 같다. 첫째, 「3-5세 연령별 누리과정」 정책의 평가 정도는 2.84∼3.66의 분포로 나타났다. 둘째, 내용단일성의 범위는 .71∼.90으로 모두 통계적으로 유의미하였다. 셋째, 문항변별도는 모든 평가지표 상위 집단의 평균이 하위 집단의 평균보다 높게 나타났으며, 통계적으로 유의미한 차이가 있는 것으로 나타났다. 넷째, 척도양호도는 확인적 요인분석 결과 모든 측정변인이 잠재변인을 설명하는 경로가 통계적으로 유의미한 것으로 나타났다. 이상의 결과를 통해 「3-5세 연령별 누리과정」 정책에 기초한 학부모용 유아교육정책 평가척도는 타당하고 신뢰로운 평가척도임이 검증되었고, 유아교육정책 및 평가 분야에 가치 있는 학술적 의미와 시사점을 제공하였다. The purpose of this study is to validate and apply the parental early childhood education policy evaluation scale by applying the Nu-ri Curriculum for 3-to-5-year-olds. In order to achieve the purpose of this study, 232 parents were selected as the research subjects. The research tool was modified and supplemented with the policy of ‘Nu-ri Curriculum for 3-to-5-year-olds’ policy developed by Kim Byung-man (2018) Respectively. The data collected in this study were descriptive statistics, construct validity, correlation analysis, reliability analysis, item discrimination, confirmatory factor analysis, and AMOS 8.0 program. The results of this study are as follows. First, the degree of evaluation of the parental early childhood education policy evaluation scale applying the ‘Nu-ri Curriculum for 3-to-5-year-olds’ policy was 2.84 ~ 3.66. The average of the public interest evaluation index of the colony was the highest, and the demographic average of the true women index was the lowest. Second, as a result of the reliability and validity test, it was proved through statistical significance that the parents' evaluation scale applying the policy of ‘Nu-ri Curriculum for 3-to-5-year-olds’ was valid and reliable evaluation scale for the early childhood education policy. This study suggests that the validity and application of the early childhood education policy evaluation scale suggests the possibility of active involvement of parents in the evaluation area of early childhood education policy.

심근 허혈-재관류 백서 모델에서 mitogen-activated protein kinase 활성화에 대한 α-lipoic acid의 효과

김연경,박은미,최준호,윤경호,유남진,이은미,김남호,정진원,김민선,박병림,오석규 圓光大學校 醫科學硏究所 2006 圓光醫科學 Vol.21 No.1

위절제술 환자의 표준진료지침 개발 및 적용 효과

김은희,김철규,이순교,김순덕,이혜옥,권정순,이경미,이민미,심순미,유용만,신종식,강은희,이상일,김병식,오성태,육정환,박수길 한국의료QA학회 2003 한국의료질향상학회지 Vol.10 No.2

Background : Gastric cancer is the most common malignant tumor in Korea. surgical operation is one of the major treatment modalities for gastric cancer patients. Therefore, gastrectomy is one of the most common procedures in General Surgery. There were variation in length of hospital stay and medical treatment for gastrectomy between three surgeons at Asan Medical Center. Clinical pathways have received considerable attention as a tool for recucing the medical practice variation, increasing the efficiency of care process, and improving the quality of care. The aim of this study was to evaluate the effect of a clinical pathway for gastrectomy in gastric cancer patients. Methods : The clinical pathway for gastrectomy was developed and implemented by a multidisciplinary group in Asan Medical Center. A computerized clinical pathway program was developed and revised after a pilot test. A total of 145 patients underwent gastrectomy by three surgeons at Asan Medical Center. We compared the length of hospital stay, patient satisfaction, and unplanned readmission rate between the pre-pathway group(n=67) and the post-pathway group(n=78). We also investigated the degree of satisfaction among the physicians and nurses who were main end-users of the clinical pathway. Results : The clinical pathway was applied to all target patients. The average length of hospital stay was shortened from 12.7days to 10.6days(p<0.01). The degree of patient satisfaction with the care process changed from 90.3% to 89.2% after the implementation of the clinical pathway, but the difference was of satistically significant(p=0.761). Unplanned readmission rate was 2.9% in the pre-pathway group. More than 90% of physicians and nurses answered that the clinical pathway had been a useful tool in their medical practice. Conclusions : The findings of the study demonstrated that implementation of the clinical pathway for gastrectomy produced substantial reduction in the length of hospital stay while improving the quality of patient outcomes. The computerized clinical pathway program can be used as one of the powerful patient management tools for reducing the practice variations and increasing the efficiency of care process in Korea hospital settings.

Algal bioassay 에 의한 조류생장 제한영양염류 결정

오희목(Hee Mock Oh),이석준(Seog June Lee),김성빈(Seong Bin Kim),박미경(Mi Kyung Park),윤병대(Byung Dae Yoon),김도한(Do Han Kim) 한국하천호수학회 1998 생태와 환경 Vol.31 No.2

Determination of Limiting Nutrient for Algal Growth by Algal Bioassay. Oh, Hee-Mock, Seog June Lee, Seong-Bin Kim, Mi- Kyung Park, Byung-Dae Yoon and Do-Han Kim (Environmental Microbiology Research Unit, Korea Research Institute of Bioscience and Biotechnology, Taejon 305-333, Korea and Water Resources Research Institute, Korea Water Resources Corporation, Taejon 305-390, Korea) Water quaiity and algal growth potential (AGP) using Microcystis aeruginosa were investigated from April to September 1997 in Daechung Reservoir. Secchi disk transparency was 1.1 m in August. Chlorophyll-a concentrations were higher than 10㎍/l at all samples. Total phosphorus concentration was in a range of 0.020∼0.027mg/l which is at the approximate value of 0.03 mg/l, the general critical level of eutrophication. These results indicate that Daechung Reservoir is in an eutrophic state except some temporary times and restricted sites. Phosphorus uptake rate of M. aeruginosa was higher in the water from the epilimnion than in the hypolimnion and also higher under light condition than under dark condition. Therefore, phosphorus uptake rate appears to depend on cellular phosphorous content and light condition, a meaning of endergonic process. Phosphorus was the most frequently limiting nutrient: phosphorus was limiting algal gowth in April and May, nitrogen, phosphorus and trace elements were the limiting factors for algae in August, and trace elements in September. If a fast growing alga, M, aeruginosa, is used as a test organism for assaying AGP, in vivo fluorescence instead of dry weight can be measured for algal biomass and incubation period can be reduced from traditional 14 days to 7 days.

통의동의 공간구조와 계획방향에 관한 연구

김은미,문병국 순천향대학교 산업기술연구소 2007 순천향 산업기술연구소논문집 Vol.13 No.2

.

Fusarium 속내의 rDNA ITS region의 RFLP 분석

민병례,이영미 상명대학교 자연과학연구소 1998 自然科學硏究 Vol.5 No.-

Fusarium section Discolor와 Roseum에 속하는 8개의 균주간의 유전적 변이를 조사하기 위하여 rDNA의 ITS 부위를 PCR을 이용하여 증폭한 후에 16개의 제한효소를 처리하여, RFLP patterns를 분석하였다. PCR 생산물의 크기는 대략 560 - 610bp로 다양하게 나타났다. 사용한 제한효소 중에서 7개만이 조사된 균주에서 절단 부위를 가지고 있었다. EcoRI와 SphI으로 처리된 band pattern은 모든 strain에서 거의 같은 양상을 나타내었다. 그러나 AluI, MspI, HaeIII, PstI 그리고 SmaI으로 처리한 후 나타난 밴드 양상은 각 균주에서 서로 다른 patterns를 보여주었다. RFLP 분석의 결과에서, Fusarium 균주의 ITS 부분은 종간과 종내 변이를 나타내었다. To investigate the genetic variation among 8 strains belonged to Fusarium Section Discolor and Roseum, after the ITS regions of the rDNA were amplified using the PCR and digested with 16 restriction enzymes, RFLP patterns were analyzed. The size of PCR products varied approximately 560-610 bp. Among these restriction endonuclease tested, seven enzymes had restriction sites in the ITS region of Fusarium species. Band patterns digested with EcoRI and SphI appeared almost same in all straines, but those with AluI, MspI, HaeIII, PstI and SmaI showed different from each other. As the result of RFLP analysis, the ITS region of the Fusarium species showed intraand interspecific variation.

일개 종합병원 입원환자의 낙상 실태 및 관련요인

양화미,천병철 한국의료QA학회 2009 한국의료질향상학회지 Vol.15 No.2

Background: Toestimate fall incidence rate and associated factorsin inpatients from a generalhospital. Method: The data werecollected from 104 fall incident reportsdeveloped by the patient safety committee in a general hospital in Seoul from 01 January 2007 to 31 December 2008. Information included general characteristicsof patients, factors related to fall, types, places,circumstances and outcomes of fall. Result: The incidence rate of fall, which was 4.4 per 1,000 total dischargedpatientsand 0.5 per 1,000 patientdays, was much lower than that of several hospitals in the United States. The difference may reflect the different incidence reporting systemof each hospital. Fall-pronepatients were, in general, ~65 years of age, had an alert mental status, were ambulatory with some a ssistance, and were dependenton and ambulatory device. High incidence of falls was associated with patients with circulatory disease. The majority of fall event susually occurredin bed orat the bedsidein thepatient's room, and occurred more of tenduring the night than during the day or evening. Risk factors of fall wereuse of drugs (antihypertensive or neuropsychiatric drugs) and environmental factors (e.g., overly highbed height, surrounding objects, inadequate fitnessshoes and slippery floor). Physical injury occurred in 43.3% of fall events, which typically required diagnosis of injuryand treatmentsuchas suturing. Riskfactorsforrepeatedfallswereuseofa neuropsychiatric drug (oddsratio=13.9) and gait disturbance (oddsratio=91.2). Risk factors for fall-related injurywerealert mental status(oddsratio=3.3 timesmore likelyto fall than thosewhoweredrowsyor ina stupor) and general weakness(odds ratio=3.3 times more likelyto fall than thosewhowerenot generally weak). Conclusion : Medicaland nursing staff shouldbe awareof the fall risk factorsof hospitalized patientsand should intensively pursue preventative strategies. Development of fall prevention education based on these resultsis recommended.