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음전하를 띤 Microsphere 에 의한 정전기적 반발력이 정밀여과에서 투과 플럭스에 미치는 영향 고찰
박정민,최성욱,방시원,함승주,김중현,김우식 한국화학공학회 2001 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.39 No.6
본 연구에서는 일정 압력하에서 음전하를 띤 microspheres의 정전기적 반발력에 따른 투과 플럭스의 변화에 관하여 고찰하였다. Microsphere의 정전기적 반발력에 관한 영향을 관찰하기 위해 표면전하 밀도(N_c: 0.45, 5.94, 10.25)가 각각 다른 poly(Styrene/Methacrylic acid) microspheres를 제조하였다. 교반속도는 300, 400, 600 rpm, 이온강도는 0.1, 0.01, 0.001로 변화시켜 실험하였다. 투과 플럭스는 표면전하밀도, 교반속도, 이온강도에 큰 영향을 받음을 알 수 있었으며, 투과 플럭스는 표면전하밀도가 클수록, 교반속도가 증가할수록, 이온강도가 작을수록 증가함을 알 수 있었다. 교반속도가 크면 막표면의 농도분극층의 두께가 얇아지기 때문에 투과 플럭스는 증가한다. 이온강도가 작을수록, 표면전하밀도(surface charge density)가 클수록 투과 플럭스가 커지는 원인은 microspheres의 정전기적 이중 층의 두께가 두꺼워짐에 따라 입자 사이의 거리가 멀어져 공극률(porosity)이 커지기 때문이다. 정상상태 플럭스 자료로부터 비 저항 정의식을 이용하여 케이크층의 공극률을 구할 수 있었다. 전하를 나타내지 않는 polystyrene microspheres의 입자크기가 480㎚일 때 농도분극 층의 이론적인 공극률은 0.211이었고, microspheres의 표면이 methacrylic acid로 완전히 개질된 것은 비 저항정의식에 의해 3.04로 계산되었다. 또한 microspheres들간의 정전기적 상호작용력이 작아지면 농도분극층의 공극률도 줄어드는 것을 알 수 있었다. The effect of the surface charge density(N_c: 0.45, 5.94, 9.14 and 10.25) and the stirrer speed(300, 400 and 600 rpm) on the variation of permeate flux was investigated in a stirred cell filled with negatively charged microspheres under constant pressure. It was found that the permeate flux depended on the surface charge density, the ionic strength and the stirrer speed. High permeate flux was obtained in the condition of high surface charge density, low ionic strength and high stirrer speed due to the force of electrostatic interaction between microspheres. The porosity of a cake layer was calculated from the steady-state flux data. It was found that the reduction of porosity was due to a decrease in repulsive interaction between microspheres.
조혈모세포이식 환자에서 침습성 진균 감염에 대한 Micafungin의 예방 효과 및 안전성
김시현,이동건,최수미,권재철,박선희,최정현,유진홍,이성은,조병식,김유진,이석,김희제,민창기,조석구,김동욱,이종욱,민우성,박종원 대한감염학회 2010 감염과 화학요법 Vol.42 No.3
Background: Micafungin, a potent inhibitor of 1,3-β-D-glucan synthase, is a novel antifungal agent of the echinocandin class. In vitro study showed that micafungin was effective against Aspergillus species as well as Candida species, but clinical data on the prophylactic efficacy against invasive fungal infections (IFIs) other than candidiasis are still lacking. Materials and Methods: We identified 60 consecutive adult hematopoietic stem cell transplantation (HSCT) recipients who received at least 3 doses of micafungin during neutropenic period. Micafungin was started as an alternative in patients who were intolerant or had adverse events (AEs) to primary prophylactic antifungal agents. We retrospectively reviewed the medical records and analyzed the efficacy and safety of micafungin for prophylaxis against IFIs. Results: The patients either had autologous (n=9) or allogeneic (n=51: 1 syngeneic, 24 sibling, 26 unrelated donor) HSCT. Itraconazole oral solution (n=58) was the most frequently used first line antifungal agent for prophylaxis and was administered for median 11 days. The most frequent cause of switch to micafungin was vomiting (n=42). The duration of neutropenia and micafungin administration was median 13 and 12 days, respectively. A successful outcome was achieved in 45 (75%) patients. Empirical antifungal therapy was initiated in 13 (22%) patients. There were 2 cases (3.3%) of breakthrough fungal infections which comprised a probable invasive pulmonary aspergillosis and a possible invasive fungal sinusitis. There was no case of invasive candidiasis. A total of 53 (88%) patients experienced at least one AE regardless of causality during micafungin administration. The most frequent AEs were hypokalemia, vomiting, diarrhea, and elevated serum aspartate aminotransferase or alanine aminotransferase. Among the aforementioned AEs, only 1 case of diarrhea could be classified as a probable relation with micafungin when causality was assessed. There was no AEs that caused discontinuation of micafungin. Conclusions: Micafungin seems to be a safe and effective agent for prophylaxis of IFIs including aspergillosis as well as candidiasis in HSCT recipients. However, further large, prospective, and randomized comparative studies are warranted for aspergillosis.
남성진,이동욱,박찬규,정시욱,김추성,조성래,구본천,양사규,이일웅,박정자 慶北大學校 醫科大學 1997 慶北醫大誌 Vol.38 No.2
Castleman' s disease is a rare, reactive lymphoid proliferation of unknown cause and pathogenesis. Depending on histololgic findings, it has been divided into two distinct histologic subtypes that include hyaline-vascular type(90%) and plasmacell type(10%). This disease occurs most commonly in mediastinal lymph nodes and cervical lymph nodes, inguinal lymph nodes respectively. But occurs rarely in retroperitoneum. We report a case of hyaline-vascular type asymptomatic Castleman' s disease of the retroperitoneum in a 47-year-old man.
조혈모세포이식 환자에서 발생한 Cytomegalovirus 질환의 특징 : 일개 대학변원에서 최근 10년간의 경험
최수미,이동건,박선희,김시현,김유진,민창기,김희제,이석,최정현,유진홍,김동욱,이종욱,민우성,신완식,김춘추 대한감염학회 2009 감염과 화학요법 Vol.41 No.1
Background : Studies on cytomegalovirus (CMV) diseases in Korean hematopoietic stem cell transplant (HSCT) recipients are lacking and do not reflect the recent trends of advances and changes. Therefore, we tried to analyze the clinical features of CMV diseases in HSCT recipients over the past 10 years at a tertiary university hospital in Korea. Methods : Retrospective review of medical records was done for all adult HSCT patients who received transplant at the Catholic HSCT Center from January 1998 to January 2008. Results : Forty-four cases (2.2%) of CMV diseases were identified. CMV pneumonia was diagnosed in 17 patients, retinitis in 16 patients, enterocolitis in 7 patients, esophagitis 1 patient, gastritis in 1 patient, duodenitis in 1 patient, and hepatitis in 1 patient. The median onset of symptom was 90 days after transplantation. Late CMV diseases accounted for 47.7%. CMV related death varied from 0 to 58.8% according to the involved organ. CMV retinitis was diagnosed relatively later in the course of transplantation mostly in patients who had chronic graft versus host disease (GVHD). On the contrary, CMV enterocolitis mainly occurred in patients who suffered from acute GVHD. The overall concurrent CMV reactivation was documented to be 63.6%: the concurrent CMV reactivation was observed only in 37.5% of patients with retinitis. Conclusions : We observed some differences in the pattern of CMV disease manifestation according to the involved organ and reconfirmed the fact that CMV pneumonia is the most common and fatal disease in HSCT recipients. Additionally, CMV retinitis was not uncommon in HSCT recipients. Since specific marker does not exist in predicting retinitis, regular ocular examination should be done thoroughly, especially in patients with chronic GVHD.
Jung, Jae Wook,Lee, Jung Seok,Kim, Jaesung,Im, Se Pyeong,Kim, Si Won,Lazarte, Jassy Mary S.,Kim, Young Rim,Chun, Jin Hong,Ha, Min Woo,Kim, Hyeong Su,Thompson, Kim D.,Jung, Tae Sung American Association of Immunologists 2020 Journal of Immunology Vol.204 No.3
<P><B>Key Points</B></P><P><OL><LI>NNV-specific VLRBs were developed using a library screening system.</LI><LI>The binding ability of this Ab was enhanced through modular engineering.</LI><LI>VLRB could be an alternative neutralizing Ab against Betanodavirus infection.</LI></OL></P><P>The variable lymphocyte receptor (VLR) mediates the humoral immune response in jawless vertebrates, including lamprey (<I>Petromyzon marinus</I>) and hagfish (<I>Eptatretus burgeri</I>). Hagfish VLRBs are composed of leucine-rich repeat (LRR) modules, conjugated with a superhydrophobic C-terminal tail, which contributes to low levels of expression in recombinant protein technology. In this study, we screened Ag-specific VLRBs from hagfish immunized with nervous necrosis virus (NNV). The artificially multimerized form of VLRB was constructed using a mammalian expression system. To enhance the level of expression of the Ag-specific VLRB, mutagenesis of the VLRB was achieved in vitro through domain swapping of the LRR C-terminal cap and variable LRR module. The mutant VLRB obtained, with high expression and secretion levels, was able to specifically recognize purified and progeny NNV, and the Ag binding ability of this mutant was increased by at least 250-fold to that of the nonmutant VLRB. Furthermore, preincubation of the Ag-specific VLRB with NNV reduced the infectivity of NNV in E11 cells in vitro, and in vivo experiment. Our results suggest that the newly developed Ag-specific VLRB has the potential to be used as diagnostic and therapeutic reagents for NNV infections in fish.</P>
Jung, Jae Wook,Lee, Jung Seok,Kim, Young Rim,Im, Se Pyeong,Kim, Si Won,Lazarte, Jassy Mary S.,Kim, Jaesung,Thompson, Kim D.,Suh, Jong Pyo,Jung, Tae Sung Elsevier 2017 Fish & shellfish immunology Vol.65 No.-
<P><B>Abstract</B></P> <P>The T cell receptor (TCR) is the binding site of antigen and is responsible for specifically activating the adaptive immune response. CD3, an essential component of the CD3-TCR complex, is known to be composed of γδ and ε chains in teleost. However, there are few monoclonal antibodies (mAb) available to identify these molecules on T cells, so we aimed to produce a mAb against CD3ε to improve our understanding of T cell immune response in olive flounder (<I>Paralichthys olivaceus)</I>. CD3ε recombinant protein was expressed in yeast, the expression of which was confirmed by SDS-PAGE, MALDI-TOF/TOF MS and Western blot analysis. A CD3ε-specific mAb 4B2 was selected, the specificity of which was examined by confocal microscopy, flow cytometry and RT-PCR, and the mAb was subsequently used to examine the CD3ε lymphocyte population in several different immune organs, with relatively high percentages of these cells seen in trunk-kidney and spleen, while lower percentages were seen in the liver and peripheral blood of olive flounder. During a viral hemorrhagic septicemia virus (VHSV) infection in olive flounder, the number of CD3ε lymphocytes was seen to gradually increase in the liver, spleen and trunk-kidney of infected fish until 7 days post infection (dpi). In peripheral blood, on the other hand, the increase in CD3ε lymphocyte numbers peaked by 3 dpi. These results suggest that CD3ε lymphocytes might be involved in the immune response against VHSV.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Recombinant CD3ε was produced using yeast expression system. </LI> <LI> Monoclonal antibody 4B2 specifically detects the CD3ε lymphocyte in olive flounder. </LI> <LI> CD3ε lymphocytes might be involved in immune response related to VHSV. </LI> </UL> </P>
A case of "Russell body gastritis" - An unusual presentation of Helicobacter pylori infection
( Si Wook Jung ),( Seung Soo Ha ),( Seong Woo Jeon ),( Byung Hun Do ),( Sang Gi Kim ),( Chang Min Cho ),( Won Young Tak ),( Young Oh Kweon ),( Sung Kook Kim ),( Yong Hwan Choi ) 대한내과학회 2005 대한내과학회 추계학술대회 Vol.69 No.10