Slide Session : OS-IFD-07 ; Infectious Disease : In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus

( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.

The Effects of Microeconomic Fundamentals on the Korean Financial Crisis

Kim,Sunghwan,Kim,Hong Kee,Kim,Jin San 한국산업경영학회 2010 한국산업경영학회 발표논문집 Vol.2010 No.1

In this study, we assess the effects of default risks and financial performance of firms on the overall risk of Korea around its financial crisis in the late 1990s and on their market values, using firm-level data from 1991 to 2007. We develop default prediction models and calculate Altman’s K scores to measure the overall risks and quality of firms respectively. Based on our own default prediction models, we have shown that the default risks of firms were extremely high before the crisis and drastically declined after the crisis. We have also found that the default risks of chaebol firms were much lower before and after the financial crisis and that their market values were much higher after the crisis, compared with those of non-chaebol firms. This implies that the Korean economy with high sovereign risks for a long time experienced the crisis due to weak firm-level fundamentals, regained its vitality due to significant improvements made by chaebol firms through the crisis and has become much stronger than before.

補强 開口部를 갖는 合成보의 擧動에 관한 硏究

崔山鎬,梁永晟,金圭石,金錫中,金弼中 江原大學校 産業技術硏究所 1991 産業技術硏究 Vol.11 No.-

A model for the strenghth of composite beams with reinfforced web openings is presented. All of theh models are based on the static theorem of ultimated strength of reinforced rectangular perforated composite beam and is compared to the test.

Paraquat의 殘留毒性에 미치는 완충액의 種類 및 光照射의 影響

金英美,申應男,李錦美,全相愛 효성여자대학교 가정대학 학도호국단 1984 家政大論集 Vol.3 No.-

완충액의 種類 및 光照射가 paraquat의 毒性低下에 미치는 影響을 검토한 結果 使用한 3種(sodium citrate, sodium phosphate, tris-HCl)의 완충액 中 sodium phosphate buffer에 paraquat를 용해하였을 때가 가장 큰 幅으로 毒性이 低下되었다. 經時的으로는 1日만에 約 60%의 細菌이 生育하였고 4日째 내는 95% 以上의 細菌이 生育함으로써 sodium phosphate buffer 中에서는 約 4日만에 paraquate의 毒性이 거의 消滅되었다. 한편, 暗所에 保有한 paraquate에 比하여 自然光구에서 1.3倍 紫外線 照射구에서 約 2倍의 生有율이 檢出되므로 光照射에 의하여 paraquat의 毒性이 보다 빨리 消滅되는 것으로 判斷되었다. Experiment was carried out to understand the effect or buffer solution and light irradiation on toxicity of paraquat against microorgnism. Paraquat most rapidly lost its toxicity in the sodium buffer solution among three kinds of tested solutions. (sodium citrate, sodium phosphate and tris-HCI) By previous incubation of paraquat in the solution for 24 hours, the organism showed about 60 percent of survival rate. The paraquat lost almost of its toxicity as showing at least 95 per cent of survival by continuous incubation for 4 days. The organism showed a survival rate of 1.3 times higher by natural light irradiation and 2.0 times higher by ultraviolet ray irradiation than control.

男子大學生의 欲求와 衣服의 評價基準과의 關係 硏究

김산옥,정명선 全南大學校家政科學硏究所 1992 生活科學硏究 Vol.2 No.-

The purpose of this study was to find out the relationship between needs(need for abasement, autonomy, exhibitionism, sex, achievement, emotion) and criteria of clothing evaluation(aesthics, status symbolism, modesty, economy, fashion) of college males, and to find out the relationship between criteria of clothing evlauation and demographic variables. (college, proportion of female in department, income.) For this study, the quetionnaire were administered to 386 college males. The data were analyzed by using Pearson's correlation, F-test, Partial correlation. The results of this study were as follows : 1) Need for achivement was strongest and need for exhibitionism, need for sex was generally strong among college males. 2) There were not significant differences in criteria of clothing evaluation according to proportion of female in department, and college too. But there were significant differences in criteria of clothing evaluation according to the income. 3) Significant positive correlation were found among the three criteria of clothing evaluation: aesthics, status symbolism, fashion. And modesty correlated significantly with economy at the .001 level. 4) There were significant positive correlation between need for abasement and modesty(p〈0.001), economy(p〈0.001). But there were negative correlation between need for abasement and fashion. Need for exhibitionism, need for sex positively correlated with aesthics, status symbolism, fashion. While need for sex negatively correlated with modesty. And need for achievement positively correlated with aesthics. 5) When status symbolism and fashion were controlled, need for exhibitionism and need for achievement positively correlated with aesthics at the .001 level. While need for sex not significantly correlated with aesthics when fashion was controlled. When aesthics was controlled, need for exhibitionism significant positively correlated fashion at the .001 level. When aesthics and fashion were controlled, need for sex correlated significantly with status symbolismat at the .01 level. After status symbolism and aesthics were controlled, need for sex correlated significantly with fashion at the .01 level and need for abasement negatively correlated with fashion at the .001 level. When fashion and status symbolism were controlled, need for exhibitionism and need for achievement significantly correlated with aesthics at the .001 level.

난자의 회수시기, BSA, Cysteine 및 Myoinositol이 개 난자의 체외성숙에 미치는 영향

김상근,이봉구,이규승 충남대학교 농업과학연구소 2007 농업과학연구 Vol.34 No.1

This study was carried out to investigate the effects of ovarian estrus stage of oocytes and supplementation of medium with BSA, cysteine and myoinositol on in vitro maturation of canine oocytes. The in vitro maturation(IVM) rate to metaphase II (M II) stage of oocytes recovered from different stage of the reproductive cycle (inactive, follicular and luteal stage) cultured in TCM-199 media were 0.0±0.0%, 10.0±4.1% and 5.7±1.6%, respectively. The IVM rate of oocytes collected from follicular stage was significantly higher in inactive or luteal stage of oocytes. The IVM rates of oocytes recovered from different stage of the reproductive cycle cultured in TCM-199 media with 5% BSA and 0.1 mM cysteine were 0.0+0.0%, 15.8+4.7%, 5.6+1.5%, respectively. The IVM rates of oocytes recovered from different stage of the reproductive cycle cultured in TCM-199 media with 5% BSA and 10 mM myoinositol were 0.0±0.0%, 18.4±4.6% and 5.7±1.9%, respectively. The IVM rate of follicular stage oocytes was significantly higher in oocytes collected from follicular stage and with cultured 5% BSA and 0.1 mM cysteine or 5% BSA and 10 mM myoinositol compared to other experimental group.

자궁경부 편평상피병소에서 인체유두종바이러스 감염과 Ki-67표출에 대한 연구

김창진,강상균,이종칠 순천향의학연구소 1997 Journal of Soonchunhyang Medical Science Vol.3 No.2

The squamous cell carcinoma of the uterine cervix is the most common malignant tumor among women in Korea. Since 1976, when a research result that human papillomavirus(HPV) infection played some role in the tumorigenesis of the uterine cervical carcinoma had been published, numerous reports supporting the result has been released. They reported that about 90% of the carcinoma had some relation with HPV infections. About 20 subtypes of HPV has been observed in the anogenital lesions. As subtypes of the virus can be grouped into three according to the potentiality of malignant tumorigenesis, high risk group (HPV16, 18), intermediate risk group(HPV31, 33)and low risk group(HPV6/11), it is very important to identify the subgroups in the precancerous and cancerous lesions of the cervix for either diagnosis or prognosis. When HPV infects the epithelial cells, it promotes cellular proliferation. The cellular proliferation can be evaluated by immunohistochemistry with the antibodies for proliferting cell nuclear antigen(PCNA) and KI-67. Because PCNA has long half-life, and can be detected 48 hours after completion of mitosis, an estimation of proliferating cells by PCNA could be inaccurate. The expression of Ki-67 antigen is more correct than PCNA for the evaluation of proliferating cells due to its short half-life and rapid degradaton after completion of the mitosis. This study was conducted to see the relation between subtypes of HPV and cell proliferation by in situ hybridization(ISH) and immunohistochemistry in 60 cases of squamous intraepithelial lesions(SILs) and invasive carcinoma of the uterine cervix. The results are as follows; 1. HPV was detected in 50 out of 60 cases (83.5%). 2. The subtypes HPV6/11, the low risk group, were detected in 78.2% and 4.5% of low grade SIL and high grade SIL respectively, while not detected in any of invasive carcinomas. 3. The subtypes HPV 16, 18, the high risk groups, were detected in 4.3%, 59.1% and 80% of low grade SIL, high grade SIL and invasive carcinomas respectively. 4. In normal squamous epithelium and squamous metaplasia, the cell fraction expressing Ki-67 were 13.5%±6.7% and 8.7%±3.5%, while in low grade SIL, high grade SIL and invasive carcinomas they were 35.8±7.9%, 66.8±11.8% and 84±13.4% resepectively. 5. There was no relation between HPV subtypes and cell fractions expressing Ki-67. Above results shows that subtypes HPV6/11 are prevalent in low grade SILs, while HPV16, 18 are common in the high grade SIL and invasive carcinoma. It seems that there is no correlation between the cell fractions expressing Ki-67 and subtypes of HPV, which suggests that cellular proliferation can be induced by other than HPV infections. As the cell fraction expressing Ki-67 increases according to the grade of SIL and invasiveness, it would be useful prognostic factor along with the identification of high risk HPV type in the uterine cervical squamous epithelial lesions.

CdGa_(2)S_(4) 결정에서의 Co^(2+) 이온의 광흡수 특성

김영산,박현,김창대 木浦大學校 基礎科學硏究所 2001 基礎科學硏究誌 Vol.19 No.-

The optical absorption spectra of Co^(2+) in CdGa_(2)S_(4)crystals propared by the melting techinque have been measured at room temperature. The spectra have been interpreted by the use of crystal field theory for the states of the 3d^(7) configuration. Also, the crystal field parameter Dq. Racah parameter B and the spin-orbit coupling parameter λ have been obtained from the observed spectra.

컴퓨터에서 쉽게 즐길 수 있는 비행 슈팅게임의 설계

강상우, 김지윤, 안성옥, 김수균, 박동원 배재대학교 공학연구소 2014 공학논문집 Vol.16 No.1

슈팅 게임(Shoting game)은 액션게임에 속해 있는 장르중 하나로 총 또는 미사일 같은 원거리 무기를 사용해 공격과 적의 공격을 피하는 게임이며[1]‘비행슈팅(Aircraft Shooting)’은 비행기를 조작해서 싸우는 게임이다[2]. 비행슈팅게임은 누구나 게임에 대해 쉽게 이해하고, 쉽게 즐길 수 있기 때문에 다른 장르의 게임들에 비해 대중화 되어 있다. 본 게임은 컴퓨터에서 쉽게 즐길 수 있도록 설계된 게임으로 우주를 배경으로 제작된 게임이며, 수많은 운석과 기뢰들을 피하고, 게임 중반부에 나오는 해적선을 파괴하고 목적지까지 도달하는 것이 게임의 목표이다. 본 게임은 Unity3D를 이용하였고, Unity3D엔진은 다른 플랫폼과의 호완성이 좋고 자체 내에서 지원해주는 기능들이 많기 때문에 많은 개발자들이 사용하고 있다.

C.V.D. 방법에 의한 Cd_0.78Zn_0.22 S박막 성장과 광전기적 특성연구

유상하,이상렬,홍광준,서상석,김혜숙,전승룡,윤은희,문종대 조선대학교 기초과학연구소 1993 自然科學硏究 Vol.16 No.1

We had grown Cd_0.78Zn_0.22S polycrystal thin films on slide substrate using chemical vapour deposition(CVD) method. We measured X-ray diffraction patterns in order to study Cd_0.78Zn_0.22S polycrystal structure. We studied its band gap using transmission curves and photocurrent and also analyzed photoluminescence using configurational coordinate model. We measured Hall effect·on this sample by Van der Pauw method and studied on carrier density and mobility depending on temperature.