miR‑1294 alleviates epithelial–mesenchymal transition by repressing FOXK1 in gastric cancer

Yaru Wang,Guangming Liu,Shijuan Sun,Junjie Qin 한국유전학회 2020 Genes & Genomics Vol.42 No.2

Background MicroRNAs (miRNAs) have been reported play critical roles in regulating tumor development and progression. Objective This study aimed to investigate the potential effect of miR-1294 in gastric cancer (GC). Methods Reverse transcription quantitative polymerase chain reaction (RT-PCR) were performed to verify the expression level of miR-1294 and Forkhead box protein K1 (FOXK1). Overall survival data of miR-1294 for GC was analysed by logrank test. Targetscan was used to screen potential target gene of miR-1294. Dual luciferase assay was assessed to investigate the relationship between miR-1294 and FOXK1. The miR-1294 overexpression and knockdown were designed to study the biological function of miR-1294. The migration and invasion of GC cell lines were investigated by wound healing and transwell assays. Western blotting were performed to verify the expression level of epithelial marker, mesenchymal markers and FOXK1. Overexpression of FOXK1 was designed to study the rescue effects of FOXK1 in SGC7901 cell. Results miR-1294 was found downregulated in GC patients and cell lines. A higher miR-1294 expression showed a significant longer overall survival than those with a lower miR-1294 expression. miR-1294 directly targets FOXK1 and regulates the expression of FOXK1. In addition, miR-1294 regulates epithelial-mesenchymal transition (EMT) by inhibiting FOXK1 in GC cells and it can be rescued by overexpression of FOXK1. Conclusion miR-1294 alleviates EMT process in GC by targeting FOXK1.

Changes in volatile organic compounds and diff erential expression of aroma-related genes during fl owering of Rosa rugosa ‘Shanxian’

Yaru Sun,Wenli Wang,Lan-yong Zhao,Chengshu Zheng,Fangfang Ma 한국원예학회 2019 Horticulture, Environment, and Biotechnology Vol.60 No.5

Rosa rugosa ‘Shanxian’, a local variety of rose in Shandong Province, was studied for its aroma characteristics, includingchanges in aroma components and diff erential expression of aroma-related genes during fl owering. The volatiles emitted fromfl owers were collected by headspace solid-phase microextraction and analyzed by gas chromatography–mass spectrometry atthe budding stage, the early, half and full opening stages, and the withering stage. Sixty-six compounds were identifi ed, andthe full opening stage contained the highest level of every single aroma component compared with the other four stages. Themajor constituents of R. rugosa ‘Shanxian’ were alcohols, esters, ketones, aldehydes, phenols, and terpenes, with alcoholsbeing the most abundant. The aroma of R. rugosa ‘Shanxian’ was mainly characterized by rich fruit aromas, fl oral aromas, andsoft plant aromas. RrAAT , RrDXR , and RrDXS genes played a key role in monoterpene biosynthesis in R. rugosa ‘Shanxian’. The temporal and spatial expression of RrAAT , RrDXR , and RrDXS were measured at diff erent fl ower developmental stages. Among the three genes, the overall expression level of RrAAT was signifi cantly higher than that of RrDXR and RrDXS andwas associated with the accumulation of acetate esters in R. rugosa , which could be used as an important candidate gene forrose metabolism. Taken together, our results demonstrated that R. rugosa ‘Shanxian’ has better aromatic characteristics thanR. rugosa ‘Fenghua’. With a particularly prominent aroma that is strong and sweet, ‘Shanxian’ should be further investigatedas a valuable breeding material that could be used to enhance genetic diversity and develop new rose varieties.

Incomplete autophagy promotes the replication of Mycoplasma hyopneumoniae

Wang Zhaodi,Wen Yukang,Zhou Bingqian,Tian Yaqin,Ning Yaru,Ding Honglei 한국미생물학회 2021 The journal of microbiology Vol.59 No.8

Autophagy is an important cellular homeostatic mechanism for recycling of degradative proteins and damaged organelles. Autophagy has been shown to play an important role in cellular responses to bacteria and bacterial replication. However, the role of autophagy in Mycoplasma hyopneumoniae infection and the pathogenic mechanism is not well characterized. In this study, we showed that M. hyopneumoniae infection significantly increases the number of autophagic vacuoles in host cells. Further, we found significantly enhanced expressions of autophagy marker proteins (LC3-II, ATG5, and Beclin 1) in M. hyopneumoniae-infected cells. Moreover, immunofluorescence analysis showed colocalization of P97 protein with LC3 during M. hyopneumoniae infection. Interestingly, autophagic flux marker, p62, accumulated with the induction of infection. Conversely, the levels of p62 and LC3-II were decreased after treatment with 3-MA, inhibiting the formation of autophagosomes, during infection. In addition, accumulation of autophagosomes promoted the expression of P97 protein and the survival of M. hyopneumoniae in PK- 15 cells, as the replication of M. hyopneumoniae was downregulated by adding 3-MA. Collectively, these findings provide strong evidence that M. hyopneumoniae induces incomplete autophagy, which in turn enhances its reproduction in host cells. These findings provide novel insights into the interaction of M. hyopneumoniae and host.

Microbiota regulation by different Akebia trifoliata fruit juices upon human fecal fermentation in vitro

Yuhao Sun,Yaru Hu,Danqi Hu,Zhuqian Xiao,Hongpeng Wang,Jun Huang,Jian-wei Mao 한국식품과학회 2023 Food Science and Biotechnology Vol.32 No.14

Three different Akebia trifoliata fruit juices were prepared, and their effects on modulation of human fecal microbiota were elucidated through an anaerobic fermentation in vitro. Results indicated that the introduction of inoculatedly-fermented Akebia trifoliata fruit juice promoted short-chain fatty acids productivity. Fecal microbiota analysis demonstrated up-regulations for abundances of Limosilactobacillus, Megamonas, Bifidobacterium, and Escherichia_Shigella, and down-regulations for numbers of Bacteroides, Prevotella_9, Parasutterella, and Sutterella. Correlation analysis confirmed relationships among sample components, short-chain fatty acids productivity, and microbial abundances, suggesting that sugars and organic acids stimulated growth of Actinobacteriota and suppressed proliferation of Proteobacteria, thus uncovering the underlying mechanism for the better ability of inoculatedly-fermented Akebia trifoliata fruit juice to regulate microbiota structure. Besides, clusters of orthologous groups of proteins analysis indicated that metabolite biosynthesis, energy metabolism, homeostasis maintenance and other physiological functions were ameliorated.

High expression of HOXC6 predicts a poor prognosis and induces proliferation and inflammation in multiple myeloma cells

Li Zhihua,Wang Yaru,Hou Xiaoxu,Guo Luyao,Li Yanling,Ma Yanping,Ma Yanping 한국유전학회 2023 Genes & Genomics Vol.45 No.7

Background Multiple myeloma (MM) is a common blood system malignance accompanied by monoclonal plasma cell hyperplasia. Homeobox C6 (HOXC6) acts as an oncogene in various cancers, but its function on MM is elusive. Objective The role of HOXC6 on MM development was clarified in this study. Methods HOXC6 expression and its clinical significance were determined in the peripheral blood samples collected from forty MM patients and thirty healthy adult volunteers. The overall survival was evaluated by Kaplan-Meier analysis with the log-rank test. Cell viability, proliferation and apoptosis were measured by CCK-8, EdU assay and Flow cytometry in U266 and MM.1R cells. Tumor growth was estimated by a xenograft assay. The apoptosis of tumor tissues was evaluated using TUNEL staining. The protein level in tissues was tested by immunohistochemistry. Results The HOXC6 expression was elevated in MM and high HOXC6 level was associated with the poor overall survival of MM. Besides, the HOXC6 expression was associated with hemoglobin level and ISS stage. Furthermore, silencing HOXC6 suppressed cell proliferation, induced cell apoptosis, and restrained the secretion of inflammatory factors (TNF-α, IL-6, and IL-8) in MM cells through inactivating the NF-κB pathway. Moreover, silencing HOXC6 suppressed the tumor growth of MM, the inflammatory factors levels, and the activation of NF-κB pathway but enhanced apoptosis in vivo. Conclusion HOXC6 was elevated in MM and associated with poor survival. Knockdown of HOXC6 suppressed proliferation, inflammation and tumorigenicity of MM cells via inactivating the NF-κB pathway. HOXC6 may be a meaningful target for MM therapy.

Investigation of the concentration characteristic of RCS during the boration process using a coupled model

Chi Xiangyu,Li Shengjie,Gu Mingzhou,Li Yaru,Zhu Xixi,Wang Naihua 한국원자력학회 2023 Nuclear Engineering and Technology Vol.55 No.8

The fluid retention effect of the Volume Control Tank (VCT) leads to a long time delay in Reactor Coolant System (RCS) concentration during the boration process. A coupled model combining a lumpedparameter sub-model and a computational fluid dynamics sub-model is currently used to investigate the concentration dynamic characteristic of RCS during the boration process. This model is validated by comparison with experimental data, and the predicted results show excellent agreement with experimental data. We provide detailed fields in VCT and concentration variations of RCS to study the interaction between mixing in VCT and the transient responses of RCS. Moreover, the impacts of the inlet flow rate, inlet nozzle diameter, original concentration, and replenishing temperature of VCT on the RCS concentration characteristic are studied. The inlet flow rate and nozzle diameter of VCT remarkably affect the RCS concentration characteristic. Too-large or too-small inlet flow rates and nozzle diameters will lead to unacceptable long delays. In this work, the optimal inlet flow rate and nozzle diameter of VCT are 5 m3 /h and 58.8 mm, respectively. Besides, the impacts of the original concentration and replenishing temperature of VCT are negligible under normal operating conditions.

Inhibition of lncRNA KCNQ1OT1 Improves Apoptosis and Chemotherapy Drug Response in Small Cell Lung Cancer by TGF-β1 Mediated Epithelial-to-Mesenchymal Transition

Deyu Li,Qin Tong,Yuane Lian,Zhizhong Chen,Yaru Zhu,Weimei Huang,Yang Wen,Qiongyao Wang,Shumei Liang,Man Li,Jianjing Zheng,Zhenhua Liu,Huanxin Liu,Linlang Guo 대한암학회 2021 Cancer Research and Treatment Vol.53 No.4

Purpose Drug resistance is one of the main causes of chemotherapy failure in patients with small cell lung cancer (SCLC), and extensive biological studies into chemotherapy drug resistance are required. Materials and Methods In this study, we performed lncRNA microarray, in vitro functional assays, in vivo models and cDNA microarray to evaluate the impact of lncRNA in SCLC chemoresistance. Results The results showed that KCNQ1OT1 expression was upregulated in SCLC tissues and was a poor prognostic factor for patients with SCLC. Knockdown of KCNQ1OT1 inhibited cell proliferation, migration, chemoresistance and promoted apoptosis of SCLC cells. Mechanistic investigation showed that KCNQ1OT1 can activate transforming growth factor-β1 mediated epithelial-to-mesenchymal transition in SCLC cells. Conclusion Taken together, our study revealed the role of KCNQ1OT1 in the progression and chemoresistance of SCLC, and suggested KCNQ1OT1 as a potential diagnostic and prognostic biomarker in SCLC clinical management.

Dirac semimetal-enabled multi-bit coding metasurface for dynamic manipulation of terahertz beams

Zhang Yonggang,Yin Kehao,Liang Lanju,Yao Haiyun,Yan Xin,Hu Xiaofei,Huang Chengcheng,Qiu Fu,Zhang Rui,Li Yuanping,Wang Yaru,Li Zhenhua,Wang Ziqun 한국물리학회 2024 Current Applied Physics Vol.58 No.-

In this study, a switchable multi-bit coding metasurface that is applied under a terahertz (THz) frequency by adjusting the Fermi level (EF) of Dirac semimetals (DSMs) is proposed. At a EF of 0.2 eV, a 1-bit coding metasurface can be applied in the 2.58–2.62 THz. At 0.3 eV, a 3-bit coding metasurface is realized at 1.88 THz, and at 0.05 eV, the phase of the coding units coincides in the 1.5–3 THz. So, different functions of the metasurface can be realized. The proposed coding metasurfaces has promising applications in terahertz communication.