Acute and repeated dose 26-week oral toxicity study of 20(S)-ginsenoside Rg3 in Kunming mice and Sprague-Dawley rats

Li, Chunmei,Wang, Zhezhe,Li, Guisheng,Wang, Zhenhua,Yang, Jianrong,Li, Yanshen,Wang, Hongtao,Jin, Haizhu,Qiao, Junhua,Wang, Hongbo,Tian, Jingwei,Lee, Albert W.,Gao, Yonglin The Korean Society of Ginseng 2020 Journal of Ginseng Research Vol.44 No.2

Background: 20(S)-ginsenoside-Rg3 (C₄₂H₇₂O₁₃), a natural triterpenoid saponin, is extracted from red ginseng. The increasing use of 20(S)-ginsenoside Rg3 has raised product safety concerns. Methods: In acute toxicity, 20(S)-ginsenoside Rg3 was singly and orally administrated to Kunming mice and Sprague-Dawley (SD) rats at the maximum doses of 1600 mg/kg and 800 mg/kg, respectively. In the 26-week toxicity study, we used repeated oral administration of 20(S)-ginsenoside Rg3 in SD rats over 26 weeks at doses of 0, 20, 60, or 180 mg/kg. Moreover, a 4-week recovery period was scheduled to observe the persistence, delayed occurrence, and reversibility of toxic effects. Results: The result of acute toxicity shows that oral administration of 20(S)-ginsenoside Rg3 to mice and rats did not induce mortality or toxicity up to 1600 and 800 mg/kg, respectively. During a 26-week administration period and a 4-week withdrawal period (recovery period), there were no significant differences in clinical signs, body weight, food consumption, urinalysis parameters, biochemical and hematological values, or histopathological findings. Conclusion: The mean oral lethal dose (LD₅₀) of 20(S)-ginsenoside Rg3, in acute toxicity, is above 1600 mg/kg and 800 mg/kg in mice and rats, respectively. In a repeated-dose 26-week oral toxicity study, the no-observed-adverse-effect level for female and male SD rats was 180 mg/kg.

Rheological behavior of acylated pepsin-solubilized collagen solutions: Effects of concentration

Conghu Li,Lian Duan,Zhenhua Tian,Wentao Liu,Guoying Li,Xiaoping Huang 한국유변학회 2015 Korea-Australia rheology journal Vol.27 No.4

Effects of concentration on the rheological behavior of acylated pepsin-solubilized collagen solutions were investigated by steady shear tests, dynamic frequency sweep, creep tests and thixotropic loop measurements in this paper. The results showed that both acylated collagen and native collagen solutions exhibited the typical pseudoplastic behavior and displayed shear thinned behavior with the increase of shear rate. With the increase of acylated collagen concentrations from 5 to 10 mg/mL, shear viscosity, elasticity modulus (G'), viscous modulus (G''), complex viscosity (η*), and the ability to resist deformation increased due to the physical entanglement, whilst loss tangent (tan δ) decreased. Additionally, with the increase of acylated collagen concentrations, the area of thixotropic loop increased from 6.94 to 44.40 watts/m3, indicating that the thixotropy of acylated collagen increased. Compared with native collagen solution, acylated collagen solution had stronger shear viscosity, η*, thixotropy, and ability to resist deformation. Furthermore, Power law model, Carreau model, Cross model, Leonov model and Burger model, were suitable for the fitting of the experimental data

Elevated TRAF4 expression impaired LPS-induced autophagy in mesenchymal stem cells from ankylosing spondylitis patients

Jinteng Li,Peng Wang,Zhongyu Xie,Rui Yang,Yuxi Li,Xiaohua Wu,Hongjun Su,Wen Deng,Shan Wang,Zhenhua Liu,Shuizhong Cen,Yi Ouyang,Yanfeng Wu,Huiyong Shen 생화학분자생물학회 2017 Experimental and molecular medicine Vol.49 No.-

Ankylosing spondylitis (AS) is a type of autoimmune disease that predominantly affects the spine and sacroiliac joints. However, the pathogenesis of AS remains unclear. Some evidence indicates that infection with bacteria, especially Gram-negative bacteria, may have an important role in the onset and progression of AS. Recently, many studies have demonstrated that mesenchymal stem cells (MSCs) dysfunction may contribute to the pathogenesis of many rheumatic diseases. We previously demonstrated that MSCs from AS patients exhibited markedly enhanced osteogenic differentiation capacity in vitro under non-inflammatory conditions. However, the properties of MSCs from AS patients in an inflammatory environment have never been explored. Lipopolysaccharide (LPS), a proinflammatory substance derived from the outer membrane of Gram-negative bacteria, can alter the status and function of MSCs. However, whether MSCs from AS patients exhibit abnormal responses to LPS stimulation has not been reported. Autophagy is a lysosome-mediated catabolic process that participates in many physiological and pathological processes. The link between autophagy and AS remains largely unknown. The level of autophagy in ASMSCs after LPS stimulation remains to be addressed. In this study, we demonstrated that although the basal level of autophagy did not differ between MSCs from healthy donors (HDMSCs) and ASMSCs, LPS-induced autophagy was weaker in ASMSCs than in HDMSCs. Specifically, increased TRAF4 expression in ASMSCs impaired LPS-induced autophagy, potentially by inhibiting the phosphorylation of Beclin-1. These data may provide further insight into ASMSC dysfunction and the precise mechanism underlying the pathogenesis of AS.

Inhibition of lncRNA KCNQ1OT1 Improves Apoptosis and Chemotherapy Drug Response in Small Cell Lung Cancer by TGF-β1 Mediated Epithelial-to-Mesenchymal Transition

Deyu Li,Qin Tong,Yuane Lian,Zhizhong Chen,Yaru Zhu,Weimei Huang,Yang Wen,Qiongyao Wang,Shumei Liang,Man Li,Jianjing Zheng,Zhenhua Liu,Huanxin Liu,Linlang Guo 대한암학회 2021 Cancer Research and Treatment Vol.53 No.4

Purpose Drug resistance is one of the main causes of chemotherapy failure in patients with small cell lung cancer (SCLC), and extensive biological studies into chemotherapy drug resistance are required. Materials and Methods In this study, we performed lncRNA microarray, in vitro functional assays, in vivo models and cDNA microarray to evaluate the impact of lncRNA in SCLC chemoresistance. Results The results showed that KCNQ1OT1 expression was upregulated in SCLC tissues and was a poor prognostic factor for patients with SCLC. Knockdown of KCNQ1OT1 inhibited cell proliferation, migration, chemoresistance and promoted apoptosis of SCLC cells. Mechanistic investigation showed that KCNQ1OT1 can activate transforming growth factor-β1 mediated epithelial-to-mesenchymal transition in SCLC cells. Conclusion Taken together, our study revealed the role of KCNQ1OT1 in the progression and chemoresistance of SCLC, and suggested KCNQ1OT1 as a potential diagnostic and prognostic biomarker in SCLC clinical management.

Effect of Liquid Mulch on the Transpiration Rate and Water Use Efficiency of Drip-irrigated Cotton

Yunguang Li,Zhenhua Wang,Jinzhu Zhang,Wenhao Li 보안공학연구지원센터 2016 International Journal of u- and e- Service, Scienc Vol.9 No.1

This study was carried out to address the ever severer white pollution issue in the cotton fields of arid areas and to primarily stop the increase of film residuals in cotton fields. The feasibility of replacing plastic films with liquid mulch in combination with drip irrigation was evaluated. A bucket experiment was conducted to investigate the effects of liquid mulch on the transpiration rate and water use efficiency (WUE) of drip-irrigated cottons. A total of 5 treatments were set up: liquid mulch (1900 kg/hm2) coupled with drip irrigation (LFD1), liquid mulch (2200 kg/hm2) coupled with drip irrigation (LFD2), liquid mulch (2500 kg/hm2) coupled with drip irrigation (LFD3), plastic film coupled with drip irrigation (PFD) and drip irrigation without mulch (NFD or control). The results showed that in the bud stage, the peak value of cotton leaf transpiration rate in LFD3 was 0.95 mmol•m-2•s-1 (or 21.99%) higher than that in control but 0.39 mmol•m-2•s-1 (or 6.89%) lower than that in PFD. The WUE in LFD3 was the highest in any cotton growth stage among the treatments. The peak value of WUE in the seedling and bud stages was 16.90% and 15.03%, respectively higher in LFD3 than in control. When used at an appropriate dosage, liquid mulch can lower the transpiration rate and significantly raise the WUE of drip-irrigated cottons, thus paving the way towards high yields. In addition, liquid mulch has no negative effects on the soil environment and thus is of important research value and good application prospect in light of drip-irrigated cottons in arid areas.

A Study of Mixing System Using Gas-Liquid Ejector

ZhenHua Jin,YouSin Jun,Tony Suryo Utomo,XueSong Li,HanShik Chung,HyoMin Jeong 한국동력기계공학회 2006 한국동력기계공학회 학술대회 논문집 Vol.- No.-

A literature review has been carried out on reactor using gas-liquid ejector. The aim of this paper is further studies to achieve deeper understanding in this field. First to investigate the influence of operating conditions and design parameters on the hydrodynamics and the mass transfer properties of a loop reactor. This paper provides a literature review on the ejectors applications in the mixing system. A number of studies are grouped and discussed in several topics. Such as the background, theory of ejector, mixing characteristics, optimization of the system. Since the high efficiencies reactor using ejector widely used in gas-liquid system, especially in a number of chemical and biochemical processes. This is due to their high efficiency in gas dispersion resulting in high mass transfer rate and low power requirements. Circulation and gas dispersion are achieved when the liquid is injected into the reactors with high velocity which entrain the gas phase and create a fine dispersion of the two phases. Thus ejector has been applied to the mixing system.

The Application analysis of Bubble Pump on Solar Water Heater System

Li Xuesong,Jin Zhenhua,Tony,Han Shik Chung,Hyo Min Jeong 한국동력기계공학회 2006 한국동력기계공학회 학술대회 논문집 Vol.- No.-

The application analysis of bubble pump on the domestic solar water heater system is presented. The system investigated in this study is a passive device, self pumping and self regulating. It was test to use the bubble pump on solar water heater system. The test experiment has been taken on the existing vacuum tube about the efficiency, working fluid temperature and pressure and circulated power. In order to check the working temperature and working pressure effectively, the bubble pump was test separated from the solar water heater. The equipment consist of the bubble pump, heater and heat exchanger. The main structure of bubble pump was design depend on the character of two phase flow. The complete system was instrumented to measure pressures, temperatures and flow-rates at various locations. The theory analysis of design bubble pump has been given and the experiment design has been included in the paper.

Parametric analysis on diagonal bracing joints connected with self-tapping screw

Zhenhua Xu,Guoliang Bai,Biao Liu,Jiarui Li,Yifan Yang 국제구조공학회 2021 Steel and Composite Structures, An International J Vol.41 No.1

In this paper, a three-dimensional finite element model (FEM) was established to study the performance of sliding resistance on the diagonal bracing joints of prefabricated steel frame (PSF) connected with self-tapping screw. In addition, the load-displacement curves of the joints were well verified by the test results, and a parametric study was conducted using the validated models to investigate the effect of steel grade, thickness of the tapped plate, the margin of bolt hole, the end and the central distance of bolt hole. The results showed that with the increase of steel grade, thickness of the tapped plate and the distance of the bolt holes, the anti-slide stiffness and the ultimate bearing capacity of the joint were significantly improved. It was recommended that steel grade should be Q345, Q390 or above. It was advisable to take the thickness of the tapped plate at 0.7d0 ~ 1.0d0, take the margin of bolt hole at 1.2d0 ~ 1.6d0, the central and the end distance of bolt hole should be greater than 2.0d0 and 1.2d0, respectively. The results could provide reference for engineering design.

A Virtual Spring Method for the Multi-robot Path Planning and Formation Control

Zhenhua Pan,Di Wang,Hongbin Deng,Kewei Li 제어·로봇·시스템학회 2019 International Journal of Control, Automation, and Vol.17 No.5

Path planning is a challenging and critical issue in robotics, which involves computing a collision-freepath between initial and target. The formation control ensures the robots’ collaborative working. To address thesetwo problems, an efficient virtual spring method for multi-robot path planning and formation control is proposed,and the interaction dynamic model is established to describe both logical and physical topology of the network. Based on the network model, the virtual spring method control law is designed, and aiming at the non-reachableand local minima problems, the virtual target search method is proposed. The robots can calculate an optimal pathto the target in the predefined formation based on the control law and the virtual target search method. Finally, aseries of simulation results confirm that the approaches proposed in this paper are feasible and efficient in the pathplanning and formation control for the multi-robot systems.

Preparation and Characterization of Hyperbranched Poly(ether ether ketone)s Suitable as Rheology Control Agents for Linear Poly(ether ether ketone)s

Zhenhua Jiang,Xiujie Li,Shuling Zhang,Fugui Zhou,Jinhui Pang,Chunfeng Zhang,Dong Sun,Jianxin Mu,Guibin Wang 한국고분자학회 2011 Macromolecular Research Vol.19 No.5

Hyperbranched poly(ether ether ketone)s (HPEEKs) that were suitable as rheology control agents for linear poly(ether ether ketone)s (LPEEKs) were first prepared via commercially available hydroquinone (A2 monomer,HQ) and synthesized 3,4',5-trifluoro-benzophenone (BB'2 monomer, TF) effortlessly. The polymerization mode of A2 + BB'2 was demonstrated by MS. Moreover, FTIR, 1H NMR, 13C NMR, and 19F NMR spectroscopy revealed the structure and degree of branching (DB) of the resulting polymers. In comparison with A2 + B3 approach, HPEEK prepared using an A2 + BB'2 strategy possessed a higher molecular weight, and improved the melt processability of LPEEK. Remarkably, with the addition of HPEEK, the mechanical properties of LPEEK increased with a HPEEK content of 5%. The improved mechanical properties were attributed to the good miscibility. Moreover, the addition of HPEEK did not decrease the thermal stability of LPEEK.