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- 저자 : Tian Yaqin (검색결과 3 건)
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Incomplete autophagy promotes the replication of Mycoplasma hyopneumoniae
Wang Zhaodi,Wen Yukang,Zhou Bingqian,Tian Yaqin,Ning Yaru,Ding Honglei 한국미생물학회 2021 The journal of microbiology Vol.59 No.8
Autophagy is an important cellular homeostatic mechanism for recycling of degradative proteins and damaged organelles. Autophagy has been shown to play an important role in cellular responses to bacteria and bacterial replication. However, the role of autophagy in Mycoplasma hyopneumoniae infection and the pathogenic mechanism is not well characterized. In this study, we showed that M. hyopneumoniae infection significantly increases the number of autophagic vacuoles in host cells. Further, we found significantly enhanced expressions of autophagy marker proteins (LC3-II, ATG5, and Beclin 1) in M. hyopneumoniae-infected cells. Moreover, immunofluorescence analysis showed colocalization of P97 protein with LC3 during M. hyopneumoniae infection. Interestingly, autophagic flux marker, p62, accumulated with the induction of infection. Conversely, the levels of p62 and LC3-II were decreased after treatment with 3-MA, inhibiting the formation of autophagosomes, during infection. In addition, accumulation of autophagosomes promoted the expression of P97 protein and the survival of M. hyopneumoniae in PK- 15 cells, as the replication of M. hyopneumoniae was downregulated by adding 3-MA. Collectively, these findings provide strong evidence that M. hyopneumoniae induces incomplete autophagy, which in turn enhances its reproduction in host cells. These findings provide novel insights into the interaction of M. hyopneumoniae and host.
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Liao, Xinyu,Li, Jiao,Suo, Yuanjie,Ahn, Juhee,Liu, Donghong,Chen, Shiguo,Hu, Yaqin,Ye, Xingqian,Ding, Tian Elsevier 2018 Food Research International Vol.105 No.-
<P>As the development of hurdle technology, cross-protection of various stresses for pathogens posed the potential risk to food safety and public health. This study tried to explore various preliminary stresses including acidity, osmosis, oxidation, heat and cold on the resistance of microbial cells toward the non-thermal plasma (NTP) exposure. The results indicated that short-term (4 h) exposure of Staphylococcus aureus and Escherichia coli to acidity, osmosis, oxidation, heat and cold stresses did not lead to the resistance to the subsequent NTP treatment. On the contrary, acidity, osmosis and heat preadaptation increased the vulnerability of E. coil cells to NTP treatment. After exposing S. aureus to osmosis, oxidation, heat and cold stress for longer period (24 h), the reduction level showed significantly (P < 0.05) higher. Interestingly, long-term (24 h) preliminary exposure of acidic stress exhibited protective effect for S. aureus against the following NTP exposure with less damage in cell membrane integrity, membrane potential and intracellular enzyme activity. It might be due to the protein production for oxidative stress response during preliminary acidic adaptation. In general, the obtained result helped to grasp better understanding of the microbial stress response to NTP treatment and provided insight for the future research in order to accelerate the development of NTP technology in food industry.</P>
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Liao, Xinyu,Li, Jiao,Muhammad, Aliyu Idris,Suo, Yuanjie,Ahn, Juhee,Liu, Donghong,Chen, Shiguo,Hu, Yaqin,Ye, Xingqian,Ding, Tian Elsevier 2018 FOOD CONTROL Vol.90 No.-
<P>Ultrasound is a promising non-thermal inactivation technique. However, ultrasound treatment alone is not very effective. In this study, combined applications of ultrasound and non-thermal plasma (NTP) were assessed for their inactivation efficacy and the physiological change on Staphylococcus aureus cells. The lethal and sublethal injury induced by individual ultrasound, NTP, ultrasound-NTP (UP) and NTP-ultrasound treatments was determined by plate count method. Then, we applied fluorescent technology to demonstrate the physiological variations of S. aureus during various treatments. NTP exposure followed by ultrasound treatment exhibited the highest inactivation rate of S. aureus, Prior NTP helped to provide enough reactive oxygen species (ROS) dissolved in the medium, and the subsequent ultrasound assisted in the injection of ROS into S. aureus cells. This accelerated the reaction between ROS and intracellular biomolecules, which led to the rapid death of the microbes. On the contrary, S. aureus cells treated with ultrasound first were more likely to develop and enhance oxidative response, allowing S. aureus to resist toward the following NTP stressor. Therefore, the findings of this study may be used to the optimization of hurdle technologies of ultrasound and NTP in practice. (C) 2018 Elsevier Ltd. All rights reserved.</P>
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