Ultrasound-enhanced Subcritical Water Extraction of Naphthoquinone Pigments from Purple Gromwell (Lithospermum erythrorhizon) to Higher Yield and Bioactivity

Ri-fu Yang,Ping-ping Huang,Tai-qiu Qiu 한국식품과학회 2013 Food Science and Biotechnology Vol.22 No.3

Naphthoquinone pigments were extracted from purple gromwell (Lithospermum erythrorhizon) via ultrasoundenhanced subcritical water extraction (USWE). USWE was compared with supercritical CO2 fluid extraction (SCE) and Soxhlet extraction (SE) on the extraction yield of naphthoquinone pigments and the components and various activities of the extracts. The results show that the extraction yield of naphthoquinone pigments by USWE was the highest, up to 0.31%, while the yields achieved by SE and SCE were 0.21 and 0.19%, respectively. The 6major compounds were detected in these 3 extract samples but the USWE sample contained the fewest impurities. The extracts obtained with USWE showed the best inhibitory effects on Escherichia coli and Staphylococcus aureus,followed by that with SE. However, the extracts obtained with SE showed the highest DPPH scavenging capacity and reducing action.

Study on the function of Helicoverpa armigera Wnt1 gene using CRISPR/Cas9 system

Fu Xiaozheng,Li Rui,Qiu Qiqi,Wang Mengke,Zhao Te,Zhou Lin 한국응용곤충학회 2022 Journal of Asia-Pacific Entomology Vol.25 No.1

The Wnt signaling pathway, as a highly conserved signaling pathway in evolution, plays an important role in many biological processes. The research of Wnt signaling pathway through gene editing has been implemented in a variety of organisms. Among the various genome editing tools available for functional genomic research, CRISPR is popular because of its ease of use and versatility. Here, we use the CRISPR/Cas9 system to knock out the HaWnt1 gene of the important agricultural pest Helicoverpa armigera to explore the impacts on embryo development. Direct injection of Cas9 protein and Wnt1-specific single guide RNA (sgRNA) into H. armigera embryos successfully induced Wnt1 gene deletion mutants, which showed high lethality, abnormal segmenta tion, defected appendages and defected pigmentation. qRT-PCR analysis revealed that the deletion of Wnt1 gene affected the expression of several genes, which were closely related to the growth and development of insects. Our results indicate that HaWnt1 signaling pathway is essential for embryonic development of H. armigera. The study of the function of HaWnt1 gene not only lays the foundation for the study of the somatic development pattern of H. armigera, but also provides a candidate gene for genetic control of H. armigera.

Quantitative Presentation of the Systematic Relationships Between S. sudanense and S. bicolor

Qiu-Wen Zhan,Ping Lin,Jie-Qin Li,Min Zu,Jun-Cheng Fan,Ya Chen,Fei Fan,Shi-fu Shu 한국초지조사료학회 2009 한국초지조사료학회 학술대회논문집 Vol.2009 No.08

Fifty-four biological characters were used for quantitative classification of sorghum and sudangrass and their relationship. Discriminant analysis of all 240 individuals of 48 accessions suggested that 100% of originally grouped cases were correctly classified. Analysis of variance results showed that the differences among 48 accessions in characters were significant at the 0.01 level. Cluster analysis showed that the 48 accessions were obviously divided into two clusters by a Euclidean distance of 17.46 as the classification standards, i.e. the genus maize and genus sorghum. The 48 accessions were divided into four clusters by a Euclidean distance of 9.86, i.e. Sorghum-Sudangrass, Sorghum related species (S. propinquum and S. halepense), Zea mexicana and Z. mays. All accessions were divided into five clusters by a Euclidean distance of 8.91, namely group Ⅰ (Sudangrass-Chinese sorghum), group Ⅱ (Non-Chinese sorghum), group Ⅲ (Sorghum related species), group Ⅳ (Zea mexicana), and group Ⅴ (Z mays).

Audio Fingerprint Retrieval Method Based on Feature Dimension Reduction and Feature Combination

( Qiu-yu Zhang ),( Fu-jiu Xu ),( Jian Bai ) 한국인터넷정보학회 2021 KSII Transactions on Internet and Information Syst Vol.15 No.2

In order to solve the problems of the existing audio fingerprint method when extracting audio fingerprints from long speech segments, such as too large fingerprint dimension, poor robustness, and low retrieval accuracy and efficiency, a robust audio fingerprint retrieval method based on feature dimension reduction and feature combination is proposed. Firstly, the Mel-frequency cepstral coefficient (MFCC) and linear prediction cepstrum coefficient (LPCC) of the original speech are extracted respectively, and the MFCC feature matrix and LPCC feature matrix are combined. Secondly, the feature dimension reduction method based on information entropy is used for column dimension reduction, and the feature matrix after dimension reduction is used for row dimension reduction based on energy feature dimension reduction method. Finally, the audio fingerprint is constructed by using the feature combination matrix after dimension reduction. When speech’s user retrieval, the normalized Hamming distance algorithm is used for matching retrieval. Experiment results show that the proposed method has smaller audio fingerprint dimension and better robustness for long speech segments, and has higher retrieval efficiency while maintaining a higher recall rate and precision rate.

Expression of ethylene biosynthetic genes during flower senescence and in response to ethephon and silver nitrate treatments in Osmanthus fragrans

Qiu Hui,Chen Yiwen,Fu Jianxin,Zhang Chao 한국유전학회 2024 Genes & Genomics Vol.46 No.4

Background Sweet osmanthus (Osmanthus fragrans) is an ornamental evergreen tree species in China, whose flowers are sensitive to ethylene. The synthesis of ethylene is controlled by key enzymes and restriction enzymes, 1-aminocyclopropane-1-carboxylic acid synthase (ACS) and 1-aminocyclopropane-1-carboxylic acid oxidase (ACO), which are encoded by multigene families. However, the key synthase responsible for ethylene regulation in O. fragrans is still unknown. Objective This study aims to screen the key ethylene synthase genes of sweet osmanthus flowers in response to ethylene regulation. Methods In this study, we used the ACO and ACS sequences of Arabidopsis thaliana to search for homologous genes in the O. fragrans petal transcriptome database. These genes were also analyzed bioinformatically. Finally, the expression levels of O. fragrans were compared before and after senescence, as well as after ethephon and silver nitrate treatments. Results The results showed that there are five ACO genes and one ACS gene in O. fragrans transcriptome database, and the phylogenetic tree revealed that the proteins encoded by these genes had high homology to the ACS and ACO proteins in plants. Sequence alignment shows that the OfACO1-5 proteins have the 2OG-Fe(II) oxygenase domain, while OfACS1 contains seven conserved domains, as well as conserved amino acids in transaminases and glutamate residues related to substrate specificity. Expression analysis revealed that the expression levels of OfACS1 and OfACO1-5 were significantly higher at the early senescence stage compared to the full flowering stage. The transcripts of the OfACS1, OfACO2, and OfACO5 genes were upregulated by treatment with ethephon. However, out of these three genes, only OfACO2 was significantly downregulated by treatment with AgNO3. Conclusion Our study found that OfACO2 is an important synthase gene in response to ethylene regulation in sweet osmanthus, which would provide valuable data for further investigation into the mechanisms of ethylene-induced senescence in sweet osmanthus flowers. Background Sweet osmanthus (Osmanthus fragrans) is an ornamental evergreen tree species in China, whose flowers are sensitive to ethylene. The synthesis of ethylene is controlled by key enzymes and restriction enzymes, 1-aminocyclopropane-1-carboxylic acid synthase (ACS) and 1-aminocyclopropane-1-carboxylic acid oxidase (ACO), which are encoded by multigene families. However, the key synthase responsible for ethylene regulation in O. fragrans is still unknown. Objective This study aims to screen the key ethylene synthase genes of sweet osmanthus flowers in response to ethylene regulation. Methods In this study, we used the ACO and ACS sequences of Arabidopsis thaliana to search for homologous genes in the O. fragrans petal transcriptome database. These genes were also analyzed bioinformatically. Finally, the expression levels of O. fragrans were compared before and after senescence, as well as after ethephon and silver nitrate treatments. Results The results showed that there are five ACO genes and one ACS gene in O. fragrans transcriptome database, and the phylogenetic tree revealed that the proteins encoded by these genes had high homology to the ACS and ACO proteins in plants. Sequence alignment shows that the OfACO1-5 proteins have the 2OG-Fe(II) oxygenase domain, while OfACS1 contains seven conserved domains, as well as conserved amino acids in transaminases and glutamate residues related to substrate specificity. Expression analysis revealed that the expression levels of OfACS1 and OfACO1-5 were significantly higher at the early senescence stage compared to the full flowering stage. The transcripts of the OfACS1, OfACO2, and OfACO5 genes were upregulated by treatment with ethephon. However, out of these three genes, only OfACO2 was significantly downregulated by treatment with AgNO3. Conclusion Our study found that OfACO2 is an important synthase gene in response to ethylene regulation in sweet osmanthus, which would provide valuable data for further investigation into the mechanisms of ethylene-induced senescence in sweet osmanthus flowers.

Anthocyanins in ‘Cabernet Gernischet’ (Vitis vinifera L. cv.) Aged Red Wine and Their Color in Aqueous Solution Analyzed by Partial Least Square Regression

Fu-Liang Han,Shou-Mei Jiang,Jian-Jun He,Qiu-Hong Pan,Chang-Qing Duan,Ming-Xia Zhang 한국식품과학회 2009 Food Science and Biotechnology Vol.18 No.3

Anthocyanins are considered one of the main color determinants in aged red wine. The anthocyanins in aged red wine made from ‘Cabernet Gernischet’ (Vitis vinifera L. cv.) grape were investigated by high performance liquid chromatography- electronic spray ionization- mass spectrometry (HPLC-ESI-MS) and their color presented in aqueous solution were evaluated using partial least square regression (PLS). The results showed that there were 37 anthocyanins identified in this wine, including 22 pyranoanthocyanins. The analysis of PLS indicated that different anthocyanins showed distinct color values: malvidin 3-O-(6-O-acetyl)-glucoside-4-vinylguaiacol (Mv3-acet-glu-vg) presented the highest color values, while malvidin 3-O-glucoside (Mv3-glu) showed least. Among the free non-acylated anthocyanins, peonidin 3-Oglucoside (Pn3-glu) showed the highest color values; the coumarylated anthocyanins presented higher color values than their corresponding acetylated anthocyanins and parent anthocyanins; pyranoanthocyanins presented also higher color values than their original anthocyanins; the color of anthocyanins depended on their structure. This work will be helpful to reveal evolution in aged red wine.

A Study on Chinese Special Regulations Concerning the Maritime Claims

Fu, Ting-Zhong,Qiu, Jin Korean Institute of Navigation and Port Research 1997 한국항해학회지 Vol.21 No.3

Under Chinese law system, the maritime law is a special branch of the civil law. For this reason, the maritime litigation shall be governed correspondently by the civil prodecure law. However, since the maritime litigation has its own special prodecure which is different from that of general procedure, there must be some special regulations to be a supplement to the civil procedure law. In this paper, a study is made on such regulations which are "The Regulations Relating to the arrest of Ships Before Litigation" and "The Regulations Concerning the Auction of Ships Which Have Been Arrested by Maritime Court for Clearing off the Debts" The aim of this paper is to describe the basic principles established in the regulations mentioned above in order to make the people who are unfamiliar with Chinese maritime legislation to be understood about Chinese special procedure adopted in maritime litigation.maritime litigation.

Exosomes Derived from Human Amniotic Mesenchymal Stem Cells Facilitate Diabetic Wound Healing by Angiogenesis and Enrich Multiple lncRNAs

Fu Shangfeng,Zhang Hongyan,Li Xiancai,Zhang Qiling,Guo Chunyan,Qiu Keqing,Feng Junyun,Liu Xiaoxiao,Liu Dewu 한국조직공학과 재생의학회 2023 조직공학과 재생의학 Vol.20 No.2

BACKGROUND: Diabetic wound healing remains a major challenge due to the impaired functionality of angiogenesis by persistent hyperglycemia. Mesenchymal stem cell exosomes are appropriate candidates for regulating the formation of angiogenesis in tissue repair and regeneration. Here, we explored the effects of exosomes derived from human amniotic mesenchymal stem cell (hAMSC-Exos) on the biological activities of human umbilical vein endothelial cells (HUVECs) treated with high glucose and on diabetic wound healing and investigate lncRNAs related to angiogenesis in hAMSC-Exos. METHODS: hAMSCs and hAMSC-Exos were isolated and identified by flow cytometry or western blot. A series of functional assays such as cell counting kit-8, scratching, transwell and tube formation assays were performed to evaluate the potential effect of hAMSC-Exos on high glucose-treated HUVECs. The effect of hAMSC-Exos on diabetic wound healing were tested by measuring wound closure rates and immunohistochemical staining of CD31. Subsequently, the lncRNAs profiles in hAMSC-Exos and hAMSCs were examined to screen the lncRNAs related to angiogenesis. RESULTS: The isolated hAMSC-Exos had a size range of 30–150 nm and were positive for CD9, CD63 and CD81. The hAMSC-Exos facilitate the functional properties of high glucose-treated HUVECs including the proliferation, migration and the angiogenic activities as well as wound closure and angiogenesis in diabetic wound. hAMSC-Exos were enriched lncRNAs that related to angiogenesis, including PANTR1, H19, OIP5-AS1 and NR2F1-AS1. CONCLUSION: Our findings demonstrated hAMSC-Exos facilitate diabetic wound healing by angiogenesis and contain several exosomal lncRNAs related to angiogenesis, which may represent a promising strategy for diabetic wound healing.

A comparison of concurrent chemoradiotherapy and radical surgery in patients with specific locally advanced cervical cancer (stage IB3, IIA2, IIICr): trial protocol for a randomized controlled study (C-CRAL trial)

Junjun Qiu,Shugen Sun,Qinqin Liu,Jie Fu,Yan Huang,Keqin Hua 대한부인종양학회 2023 Journal of Gynecologic Oncology Vol.34 No.5

Objective: Genetic high-risk assessment combines hereditary breast, ovarian and pancreatic cancer into one syndrome. However, there is a lack of data for comparing the germline mutational spectrum of the cancer predisposing genes between these three cancers. Methods: Patients who met the criteria of the hereditary breast, ovarian and pancreatic cancer were enrolled and received multi-gene sequencing. Results: We enrolled 730 probands: 418 developed breast cancer, 185 had ovarian cancer, and 145 had pancreatic cancer. Out of the 18 patients who had two types of cancer, 16 had breast and ovarian cancer and 2 had breast and pancreatic cancer. A total of 167 (22.9%) patients had 170 mutations. Mutation frequency in breast, ovarian and pancreatic cancer was 22.3%, 33.5% and 17.2%, respectively. The mutation rate was significantly higher in patients with double cancers than those with a single cancer (p<0.001). BRCA1 and BRCA2 were the most dominant genes associated with hereditary breast and ovarian cancer, whereas ATM was the most prevalent gene related to hereditary pancreatic cancer. Genes of hereditary colon cancer such as lynch syndrome were presented in a part of patients with pancreatic or ovarian cancer but seldom in those with breast cancer. Families with a history of both ovarian and breast cancer were associated with a higher mutation rate than those with other histories. Conclusion: The mutation spectrum varies across the three cancer types and family histories. Our analysis provides guidance for physicians, counsellors, and counselees on the offer and uptake of genetic counseling.

On Legal System Regarding Actual Carrier -At the angle of Voyage Charter Party-

( Jin Qiu ),( Fu Ting Zhong ) 한국해법학회 2013 韓國海法學會誌 Vol.35 No.2

중국 해상법상 실제운송인 제도는 함부르크 규칙으로부터 도입되었으며, 해상물건운송계약에 관한 제4장의 기본 원칙 중의 하나로 규정되어 있다. 이러한 법전상의 규정 순서는 실제운송인 제도가 모든 종류의 해상물건운송계약에 적용되어야 함을 의미한다. 그러나 해사 판례 중에는 이제도가 항해용선계약에는 적용되지 않고 선하증권에만 적용된다고 판시한 것들이 있다. 이 논문에서 필자들은 실제운송인의 의의를 네 가지 차원에서 논의하고, 이 제도가 선하증권만이 아니라 용선계약에도 적용되어야 한다는 점을 주장한다. In Chinese Maritime Code, the system of actual carrier was introduced from <Hamburg Rules>. the concept of actual carrier is provided in Section one which is the “Basic Principles” of Chapter four (Contract of Carriage of Goods by Sea).Such arrangement about legal structure means that the system of actual carrier should apply to all kinds of contract of carriage of goods by sea. Nevertheless, in the maritime judicial practice, some persons argue that this system applies only to the bill of lading rather than voyage charter party. In this article, the authors discussed the connotation of this system at four levels and pointed out that such system should apply not only to the B/L but also to the charter party, otherwise, the significance for regulating such system shall be diminished.