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      • KCI등재

        The influence of surface lithium residue to the performance of LiNi0.9Co0.05Mn0.05O2 cathode materials

        Liu Junjie,Chu Chenxiao,Qin Xianzhong,Meng Weisong,Xu Xinrui,Wang Bo,Cai Feipeng 한국세라믹학회 2023 한국세라믹학회지 Vol.60 No.3

        High nickel ternary cathode materials (Ni ≥ 90%) have great potential for application as power batteries in electric vehicles and have become a hot spot for research on cathode materials. However, the residual Li on the surface of the high Ni ternary cathode materials prepared by solid-phase sintering is one of the main reasons affecting their electrochemical performance. It is found that Li1 + x(Ni0.9Co0.05Mn0.05)1-xO2 with x = 0.3, which has a discharge-specific capacity of 205.74 mAh g −1 at 2.7–4.3 V, 0.1C and 184.1 mAh g −1 at 1 C, retained 89% of its initial discharge capacity after 100 cycles at 1 C. The discharge-specifi c capacity at 10 C was 153.14 mAh g −1. In addition, the Li residue of NCM90-1.03 was 17,189.959 ppm, and the total alkalinity was 4196.896 ppm. The results by X-ray diff raction (XRD) and scanning electron microscope (SEM) showed that when the Li excess was 0.3%, it was possible to obtain uniform particle size, a wider Li layer, and reduced cation mixing. Therefore, choosing the right amount of excess Li can stimulate the application prospect of NCM90 in new energy vehicles.

      • Structural Conservation and Food Habit-related Liver Expression of Uncoupling Protein 2 Gene in Five Major Chinese Carps

        Liao, Wan-Qin,Liang, Xu-Fang,Wang, Lin,Fang, Ling,Lin, Xiaotao,Bai, Junjie,Jian, Qing Korean Society for Biochemistry and Molecular Biol 2006 Journal of biochemistry and molecular biology Vol.39 No.4

        The full-length cDNA of grass carp (Ctenopharyngodon idellus) and silver carp (Hypophthalmichthys molitrix) uncoupling protein 2 (UCP2) was obtained from liver. The grass carp UCP2 cDNA was determined to be 1152 bp in length with an open reading frame that encodes 310 amino acids. Five introns (Intron 3, 4, 5, 6 and 7) in the translated region, and partial sequence of Intron 2 in the untranslated region of grass carp UCP2 gene were also obtained. Gene structure comparison between grass carp and mammalian (human and mouse) UCP2 gene shows that, the UCP2 gene structure of grass carp is much similar to that of human and mouse. Partial UCP2 cDNA sequences of bighead carp (Aristichthys nobilis) and mud carp (Cirrhinus molitorella), were further determined. Together with the common carp (Cyprinus carpio) UCP2 sequence from GenBank (AJ243486), multiple alignment result shows that the nucleotide and amino acid sequences of the UCP2 gene, were highly conserved among the five major Chinese carps that belong to four subfamilies. Using beta-actin as control, the ratio UCP2/beta-actin mRNA (%) was determined to be $149.4{\pm}15.6$ (common carp), $127.4{\pm}22.1$ (mud carp), $96.7{\pm}12.7$ (silver carp), $94.1{\pm}26.8$ (bighead carp) and $63.7{\pm}16.2$ (grass carp). The relative liver UCP2 expression of the five major Chinese carps, shows a close relationship with their food habit: benthos and detrituseating fish (common carp and mud carp) > planktivorious fish (silver carp and bighead carp) > herbivorious fish (grass carp). We suggest that liver UCP2 might be important for Chinese carps to detoxify cyanotoxins and bacteria in debris and plankton food.

      • KCI등재

        miR‑1294 alleviates epithelial–mesenchymal transition by repressing FOXK1 in gastric cancer

        Yaru Wang,Guangming Liu,Shijuan Sun,Junjie Qin 한국유전학회 2020 Genes & Genomics Vol.42 No.2

        Background MicroRNAs (miRNAs) have been reported play critical roles in regulating tumor development and progression. Objective This study aimed to investigate the potential effect of miR-1294 in gastric cancer (GC). Methods Reverse transcription quantitative polymerase chain reaction (RT-PCR) were performed to verify the expression level of miR-1294 and Forkhead box protein K1 (FOXK1). Overall survival data of miR-1294 for GC was analysed by logrank test. Targetscan was used to screen potential target gene of miR-1294. Dual luciferase assay was assessed to investigate the relationship between miR-1294 and FOXK1. The miR-1294 overexpression and knockdown were designed to study the biological function of miR-1294. The migration and invasion of GC cell lines were investigated by wound healing and transwell assays. Western blotting were performed to verify the expression level of epithelial marker, mesenchymal markers and FOXK1. Overexpression of FOXK1 was designed to study the rescue effects of FOXK1 in SGC7901 cell. Results miR-1294 was found downregulated in GC patients and cell lines. A higher miR-1294 expression showed a significant longer overall survival than those with a lower miR-1294 expression. miR-1294 directly targets FOXK1 and regulates the expression of FOXK1. In addition, miR-1294 regulates epithelial-mesenchymal transition (EMT) by inhibiting FOXK1 in GC cells and it can be rescued by overexpression of FOXK1. Conclusion miR-1294 alleviates EMT process in GC by targeting FOXK1.

      • KCI등재

        Effects of Isosorbide on the Microphase Separation and Properties of Waterborne Polyurethane Coatings

        Jun Hu,Can Tao,Aining Yuan,Junjie Bao,Qin Cheng,Gewen Xu,Yiping Huang 한국고분자학회 2019 폴리머 Vol.43 No.2

        Series of waterborne polyurethanes (WPUs) containing poly(tetra methylene glycol) (PTMG), 4,4-dicyclohexylmethane diisocyanate (HMDI), 2,2-dimethylolpropionic acid (DMPA), 2,2,4-trimethyl-1,3-pentanediol (TMPD), isosorbide (ISO) and ethylenediamine (EDA) were synthesized. The effects of ISO on the structure and properties of WPUs were studied by gel permeation chromatography (GPC), Fourier-transform infrared spectroscopy (FTIR), differential scanning calorimetry (DSC), dynamic mechanical analyzer (DMTA), X-ray diffraction (XRD), thermogravimetry (TG), mechanical test, chemical resistance and hardness test. The deconvolution analysis of FTIR of WPUs revealed that the hydrogen bond interaction in the WPUs increased with the increase of ISO content. Furthermore, the degree of microphase separation was increased with the increase of hydrogen bond interaction, which was confirmed by DSC, XRD and DMTA test. The results demonstrated that the addition of ISO can increase the hydrogen bond interaction between the hard and hard segments in WPUs, which improved the mechanical properties of the WPU films. The highest tensile strength was obtained of 59.67 MPa when the content of isosorbide was 10.35 wt%. Moreover, the ISO was helpful to improve the chemical resistance and hardness of the coatings by the comprehensive performance analysis.

      • KCI등재

        Preliminary conceptual design of a small high-flux multi-purpose LBE cooled fast reactor

        Xiong Yangbin,Duan Chengjie,Zeng Qin,Ding Peng,Song Juqing,Zhou Junjie,Xu Jinggang,Yang Jingchen,Li Zhifeng 한국원자력학회 2022 Nuclear Engineering and Technology Vol.54 No.8

        The design concept of a Small High-flux Multipurpose LBE(Lead Bismuth Eutectic) cooled Fast Reactor (SHMLFR) was proposed in the paper. The primary cooling system of the reactor is forced circulation, and the fuel element form is arc-plate loaded high enrichment MOX fuel. The core is cylindrical with a flux trap set in the center of the core, which can be used as an irradiation channel. According to the requirements of the core physical design, a series of physical design criteria and constraints were given, and the steady and transient parameters of the reactor were calculated and analyzed. Regarding the thermal and hydraulic phenomena of the reactor, a simplified model was used to conduct a preliminary analysis of the fuel plates at special positions, and the temperature field distribution of the fuel plate with the highest power density under different coolant flow rates was simulated. The results show that the various parameters of SHMLFR meet the requirements and design criteria of the physical design of the core and the thermal design of the reactor. This implies that the conceptual design of SHMLFR is feasible

      • KCI등재

        Modulatory Potential of LncRNA Zfas1 for Inflammation and Neuronal Apoptosis in Temporal Lobe Epilepsy

        Chuan He,Caixia Su,Wentong Zhang,Qin Zhou,Xu Shen,Junjie Yang,Naixian Shi 연세대학교의과대학 2021 Yonsei medical journal Vol.62 No.3

        Purpose: This study aimed to elucidate whether lncRNA ZFAS1 is involved in neuronal apoptosis and inflammation in temporal lobe epilepsy (TLE). Materials and Methods: Ninety-six TLE patients were recruited, and their peripheral venous blood was gathered to determine Zfas1 expression with polymerase chain reaction. Neurons were separated from hippocampal tissue of newborn SD rats, and siZfas1 or pcDNA3.1-Zfas1 was transfected into the neurons. Inflammatory cytokines released by neurons were determined, and neuronal activities were evaluated through MTT assay, colony formation assay, and flow cytometry. Results: Serum levels of Zfas1 were higher in TLE patients than in healthy controls (p<0.05). Furthermore, Zfas1 expression in neurons was raised by pcDNA3.1-Zfas1 and declined after silencing of Zfas1 (p<0.05). Transfection of pcDNA-Zfas1 weakened the viability and proliferation of neurons and increased neuronal apoptosis (p<0.05). Meanwhile, pcDNA3.1-Zfas1 transfection promoted lipopolysaccharide-induced release of cytokines, including tumor necrosis factor-α, interleukin (IL)-1, IL-6, and intercellular adhesion molecule-1 (p<0.05), and boosted NF-κB activation by elevating the expression of NF-κB p65, pIκBα, and IKKβ in neurons (p<0.05). Conclusion: Our results indicated that lncRNA ZFAS1 exacerbates epilepsy development by promoting neuronal apoptosis and inflammation, implying ZFAS1 as a promising treatment target for epilepsy.

      • SCIESCOPUSKCI등재

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