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Yaru Sun,Wenli Wang,Lan-yong Zhao,Chengshu Zheng,Fangfang Ma 한국원예학회 2019 Horticulture, Environment, and Biotechnology Vol.60 No.5
Rosa rugosa ‘Shanxian’, a local variety of rose in Shandong Province, was studied for its aroma characteristics, includingchanges in aroma components and diff erential expression of aroma-related genes during fl owering. The volatiles emitted fromfl owers were collected by headspace solid-phase microextraction and analyzed by gas chromatography–mass spectrometry atthe budding stage, the early, half and full opening stages, and the withering stage. Sixty-six compounds were identifi ed, andthe full opening stage contained the highest level of every single aroma component compared with the other four stages. Themajor constituents of R. rugosa ‘Shanxian’ were alcohols, esters, ketones, aldehydes, phenols, and terpenes, with alcoholsbeing the most abundant. The aroma of R. rugosa ‘Shanxian’ was mainly characterized by rich fruit aromas, fl oral aromas, andsoft plant aromas. RrAAT , RrDXR , and RrDXS genes played a key role in monoterpene biosynthesis in R. rugosa ‘Shanxian’. The temporal and spatial expression of RrAAT , RrDXR , and RrDXS were measured at diff erent fl ower developmental stages. Among the three genes, the overall expression level of RrAAT was signifi cantly higher than that of RrDXR and RrDXS andwas associated with the accumulation of acetate esters in R. rugosa , which could be used as an important candidate gene forrose metabolism. Taken together, our results demonstrated that R. rugosa ‘Shanxian’ has better aromatic characteristics thanR. rugosa ‘Fenghua’. With a particularly prominent aroma that is strong and sweet, ‘Shanxian’ should be further investigatedas a valuable breeding material that could be used to enhance genetic diversity and develop new rose varieties.
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Circ_0068655 Promotes Cardiomyocyte Apoptosis via miR-498/PAWR Axis
Chai Qiaoying,Zheng Mingqi,Wang Le,Wei Mei,Yin Yajuan,Ma Fangfang,Li Xinping,Zhang Haijun,Liu Gang 한국조직공학과 재생의학회 2020 조직공학과 재생의학 Vol.17 No.5
Background: The cardiomyocyte apoptosis is considered as one of major contributions to cardiac remodeling after myocardial infarction (MI). Numerous studies find that circular RNAs (circRNAs) play pivotal roles in a variety of biological functions. However, the role of circ_0068655 in MI and human induced pluripotent stem-derived cardiomyocytes (HCMs) remains unknown. Methods: The expression of circ_0068655, miR-498, and PRKC apoptosis WT1 regulator (PAWR) in human MI heart tissues and hypoxia subjected HCMs was evaluated with qRT-PCR and Western blot. The effects of circ_0068655 on hypoxia-induced apoptotic death and cell migration in HCMs were evaluated with qRT-PCR, cell viability, cell death ELISA (POD), and Caspase-3 activity assay, and Trans-well assay, respectively. Furthermore, luciferase assay, qRT-PCR, biotin-labeled miRNA pulldown assay, and Western blot were employed in the functional studies. Results: We found that the expression of circ_0068655 and PAWR was enhanced in MI patients and hypoxia subjected HCMs; by contrast, the expression of miR-498 decreased. Inhibited expression of circ_0068655 in HMCs counteracted hypoxia-induced apoptotic death and impaired cell migration, in sharp contrast to circ_0068655 knockdown. We identified that circ_0068655 sponged an endogenous miR-498 to sequester and inhibit its activity, leading to the increased PAWR expression. Conclusions: Our findings reveal that the expression of circ_0068655 can promote cardiomyocyte apoptosis through the modulation of miR-498-PAWR axis in vitro, which highlights the diagnostic and therapeutic value of circ_0068655 in patients with MI.
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