Changes in volatile organic compounds and diff erential expression of aroma-related genes during fl owering of Rosa rugosa ‘Shanxian’

Yaru Sun,Wenli Wang,Lan-yong Zhao,Chengshu Zheng,Fangfang Ma 한국원예학회 2019 Horticulture, Environment, and Biotechnology Vol.60 No.5

Rosa rugosa ‘Shanxian’, a local variety of rose in Shandong Province, was studied for its aroma characteristics, includingchanges in aroma components and diff erential expression of aroma-related genes during fl owering. The volatiles emitted fromfl owers were collected by headspace solid-phase microextraction and analyzed by gas chromatography–mass spectrometry atthe budding stage, the early, half and full opening stages, and the withering stage. Sixty-six compounds were identifi ed, andthe full opening stage contained the highest level of every single aroma component compared with the other four stages. Themajor constituents of R. rugosa ‘Shanxian’ were alcohols, esters, ketones, aldehydes, phenols, and terpenes, with alcoholsbeing the most abundant. The aroma of R. rugosa ‘Shanxian’ was mainly characterized by rich fruit aromas, fl oral aromas, andsoft plant aromas. RrAAT , RrDXR , and RrDXS genes played a key role in monoterpene biosynthesis in R. rugosa ‘Shanxian’. The temporal and spatial expression of RrAAT , RrDXR , and RrDXS were measured at diff erent fl ower developmental stages. Among the three genes, the overall expression level of RrAAT was signifi cantly higher than that of RrDXR and RrDXS andwas associated with the accumulation of acetate esters in R. rugosa , which could be used as an important candidate gene forrose metabolism. Taken together, our results demonstrated that R. rugosa ‘Shanxian’ has better aromatic characteristics thanR. rugosa ‘Fenghua’. With a particularly prominent aroma that is strong and sweet, ‘Shanxian’ should be further investigatedas a valuable breeding material that could be used to enhance genetic diversity and develop new rose varieties.

miR‑1294 alleviates epithelial–mesenchymal transition by repressing FOXK1 in gastric cancer

Yaru Wang,Guangming Liu,Shijuan Sun,Junjie Qin 한국유전학회 2020 Genes & Genomics Vol.42 No.2

Background MicroRNAs (miRNAs) have been reported play critical roles in regulating tumor development and progression. Objective This study aimed to investigate the potential effect of miR-1294 in gastric cancer (GC). Methods Reverse transcription quantitative polymerase chain reaction (RT-PCR) were performed to verify the expression level of miR-1294 and Forkhead box protein K1 (FOXK1). Overall survival data of miR-1294 for GC was analysed by logrank test. Targetscan was used to screen potential target gene of miR-1294. Dual luciferase assay was assessed to investigate the relationship between miR-1294 and FOXK1. The miR-1294 overexpression and knockdown were designed to study the biological function of miR-1294. The migration and invasion of GC cell lines were investigated by wound healing and transwell assays. Western blotting were performed to verify the expression level of epithelial marker, mesenchymal markers and FOXK1. Overexpression of FOXK1 was designed to study the rescue effects of FOXK1 in SGC7901 cell. Results miR-1294 was found downregulated in GC patients and cell lines. A higher miR-1294 expression showed a significant longer overall survival than those with a lower miR-1294 expression. miR-1294 directly targets FOXK1 and regulates the expression of FOXK1. In addition, miR-1294 regulates epithelial-mesenchymal transition (EMT) by inhibiting FOXK1 in GC cells and it can be rescued by overexpression of FOXK1. Conclusion miR-1294 alleviates EMT process in GC by targeting FOXK1.

Microbiota regulation by different Akebia trifoliata fruit juices upon human fecal fermentation in vitro

Yuhao Sun,Yaru Hu,Danqi Hu,Zhuqian Xiao,Hongpeng Wang,Jun Huang,Jian-wei Mao 한국식품과학회 2023 Food Science and Biotechnology Vol.32 No.14

Three different Akebia trifoliata fruit juices were prepared, and their effects on modulation of human fecal microbiota were elucidated through an anaerobic fermentation in vitro. Results indicated that the introduction of inoculatedly-fermented Akebia trifoliata fruit juice promoted short-chain fatty acids productivity. Fecal microbiota analysis demonstrated up-regulations for abundances of Limosilactobacillus, Megamonas, Bifidobacterium, and Escherichia_Shigella, and down-regulations for numbers of Bacteroides, Prevotella_9, Parasutterella, and Sutterella. Correlation analysis confirmed relationships among sample components, short-chain fatty acids productivity, and microbial abundances, suggesting that sugars and organic acids stimulated growth of Actinobacteriota and suppressed proliferation of Proteobacteria, thus uncovering the underlying mechanism for the better ability of inoculatedly-fermented Akebia trifoliata fruit juice to regulate microbiota structure. Besides, clusters of orthologous groups of proteins analysis indicated that metabolite biosynthesis, energy metabolism, homeostasis maintenance and other physiological functions were ameliorated.

Real-time comprehensive image processing system for detecting concrete bridges crack

Weiguo Lin,Yichao Sun,Qiaoning Yang,Yaru Lin 사단법인 한국계산역학회 2019 Computers and Concrete, An International Journal Vol.23 No.6

Cracks are an important distress of concrete bridges, and may reduce the life and safety of bridges. However, the traditional manual crack detection means highly depend on the experience of inspectors. Furthermore, it is time-consuming, expensive, and often unsafe when inaccessible position of bridge is to be assessed, such as viaduct pier. To solve this question, the real-time automatic crack detecting system with unmanned aerial vehicle (UAV) become a choice. This paper designs a new automatic detection system based on real-time comprehensive image processing for bridge crack. It has small size, light weight, low power consumption and can be carried on a small UAV for real-time data acquisition and processing. The real-time comprehensive image processing algorithm used in this detection system combines the advantage of connected domain area, shape extremum, morphology and support vector data description (SVDD). The performance and validity of the proposed algorithm and system are verified. Compared with other detection method, the proposed system can effectively detect cracks with high detection accuracy and high speed. The designed system in this paper is suitable for practical engineering applications.

Enhancing Electrocatalytic Production of H2O2 by Modulating Coordination Environment of Cobalt Center

Guoling Wu,Zhongjie Yang,Tianlin Zhang,Yali Sun,Chang Long,Yaru Song,Shengbin Lei,Zhiyong Tang 대한화학회 2021 Bulletin of the Korean Chemical Society Vol.42 No.8

As an environmentally friendly oxidant, H2O2 is widely utilized in various fields; however, its production methods remain limited to the chemical anthraquinone process. Alternatively, electrocatalytic oxygen reduction possesses numerous notable advantages (e.g., cost-effectiveness, small-scale, and distributed nature). As electrocatalytic oxygen reduction has been widely investigated in the fields of fuel cells and metal-air batteries, the mechanism of the 2e?-ORR pathway for producing H2O2 is not sufficiently clear. Herein, we explore the effect of the cobalt (Co) coordination environment on the electrochemical production of H2O2. The detailed investigation on N-, P-, and S-coordinated Co catalysts (Co1N1N3, Co1P1N3, and Co1S1N3) demonstrates that changing the coordination environment evidently affects the H2O2 selectivity, and the S-coordinated Co exhibits the best catalytic performance. This finding would lead to the design and selection of catalysts at atomic level for producing H2O2 via electrocatalytic oxygen reduction.

Energy efficiency onboard hydraulic power for quadruped robot based on high-low double pumps supply

Zisen Hua,Zhiwei Zhang,Hui Chai,Yibin Li,Xianhua Li,Yaru Sun 대한기계학회 2023 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.37 No.9

This work presents a high energy efficiency onboard hydraulic system for quadruped robot. Firstly, based on the rhythmic motion and mechanical characteristics of leg joints, the energy supply mode that supplies low-pressure oil and high-pressure oil separately for the swing phase and supporting phase is used, and the switch between different oil circuits for each legs is implemented by two digital on-off valves. What’s more, to obtain the pressure dynamics characteristics and energy consumption model, the mathematical models is deduced, and the analysis results illustrates that a better rate response can be achieved by adjusting time offset reasonable properly. Finally, the simulations and experiments are conducted to prove that the proposed system shows better energy efficiency compared with that of single pump supply pressure system, meanwhile, the fast dynamic response and the high tracking accuracy are also maintained.