Optimal Fermentation Conditions for Enhanced GlutathioneProduction by Saccharomyces cerevisiae FF-8

Jae-Young Cha1,,Jin-Chul Park,Beong-Sam Jeon,Young-Choon Lee,Young-Su Cho 한국미생물학회 2004 The journal of microbiology Vol.42 No.1

The influence of feedstock amino acids, salt, carbon and nitrogen sources on glutathione production by Saccharomyces cerevisiae FF-8 was investigated. Glucose, yeast extract, KH2PO4, and L-cysteine were found to be suitable feedstock. Highest glutathione production was obtained after cultivation with shaking for 72 h in a medium containing glucose 3.0% (w/v), yeast extract 3.0%, KH2PO4 0.06% and L-cysteine 0.06%. The glutathione concentration achieved using this medium increased 2.27-fold to 204 mg/l compared to YM basal medium.

한국판 대칭 및 정리정돈 성향 척도(K-SOAQ)의 표준화 연구

차경렬,임준석,박준영,이수영,김광욱,노대영,김세주,구민성,김찬형 大韓神經精神醫學會 2008 신경정신의학 Vol.47 No.3

Objectives : The Symmetry, Ordering and Arranging Questionnaire (SOAQ) has been reported as a valid tool for the assessment of symmetry, ordering and arranging compulsive behaviors of patients with obsessive compulsive disorder. It was introduced by Radomsky and Rachman in 2004. The aim of this study was to assess the reliability, validity and psychometric properties of the Korean-version of the SOAQ (K-SOAQ). Methods : Two hundreds twenty eight under-graduate college students were enrolled in this study. They were assessed with K-SOAQ, Obsessive-Compulsive Inventory-Revised (OCI-R), Beck Depression Inventory (BDI) and Beck Anxiety Inventory (BAI). Statistical analysis was done using calculation of Cronbach’s alpha, Spearman Correlation Coefficient and Principal Components Analysis. Results : There were no significant differences in sex, education, and total scores of BDI and BAI. The Cronbach’s alpha coefficient of K-SOAQ was high, Cronbach’s α=0.96. There was strong evidence for a one-factor solution, accounting for 60.3% of the variance. Convergent validity was examined through correlations between the SOAQ and the OCI-R (r=0.61). Divergent validity was examined through correlations between the SOAQ and two self report measures; BDI (r=0.12) and BAI (r=0.19). Conclusion : The K-SOAQ showed good reliability and validity for the assessment of severity of compulsive characteristics regarding symmetry, ordering and arranging. The K-SOAQ is a useful instrument for assessing compulsive symptoms related with symmetry, ordering and arranging in Korea.

10kW BIPV의 PCS 특성에 관한 연구

차인수,윤정필,윤영찬 東新大學校 工業技術硏究所 2004 工業技術硏究 Vol.10 No.-

BIPV(Building Integrated PV) system that is united on constituent outer covering of building can expect dual effects that reduce expenses for establishment of PV system by adding new function as outer covering material of building except producing the electricity. Also, it is a profitable technology in the economical field because it does not need the building site for a special purpose for the establishment as existing stand along PV system. In this paper, it was simulated through the theoretical consideration about output characteristic analysis of PCS and web-based monitoring of 10kW BIPV, finally was examined the validity through the experiment. BIPV system that is proposed in this paper, was established in BIC(Biotechnology Industrialization Center) of Dongshin University, composed with PCS and Web-monitoring system.

오로토산 유발 지방산의 간장 트리아실글리세롤 축적간 지방산 조성의 변화

차재영,김경숙,조영수 한국식품영양학회 1998 韓國食品營養學會誌 Vol.11 No.5

본 연구는 오르트산 투여가 흰쥐의 지방간 유발 형성과정에서 간장 및 혈청의 각 지질과 간세포의 지방산 조성에 대하여 경시적으로 함량 변화를 분석하였다. 그 결과 오로트산 투여 흰쥐의 간장 지질의 구성 지방산 중에서 리놀레산 (18:2, n-6)이 모든 각 지방질 중 가장 많이 증가하였다. 리놀레산은 간세포의 소포체 및 골지체의 트리아실글리세롤에서도 증가하였다. 이 지방산 패턴을 경시적으로 분석한 결과, 간장 트리아실글리세롤에서는 오로트산 투여 1일째부터 증가하고, 디아실글리세롤에서도 같은 결과를 나타냈다. 그러나 혈청 트리아실글리세롤에서는 오로트산 투여 1일째에서는 증가하였으나, 2일째부터는 증가율이 둔화되었고, 10일째에서는 대조군보다 저하하였다. 한편, 지방산 중에서 올레인산(18:1, n-9)은 간장 콜레스테릴 에스테르에서만 현저히 상승하였으나, 다른 것에서는 차이가 없었다. 간장 트리아실글리세롤, 디아실글리세롤, 콜레스테릴 에스테릴의 구성 지방산 중에서는 14:0, 16:0, 18:0의 포화지방산은 오르트산 투여로 저하하였고, 역으로 혈청 트리아실글리세롤에서는 이런 지방산이 증가하였다. 이러한 결과로부터, 오르트산 유발 지방간에 있어서 간장 트리아실글리세롤이 축적될 때, 간장지질의 구성 지방산 중에서 18:2의 비율이 현저하게 증가한바, 지방산의 대사 저해와, 간장에서 혈청으로 이행하는 경로가 저해되고 있는 것으로 나타났다. Dietary orotic acid is known to induce the fatty liver. Fatty acid profiles in the lipid fraction of the liver and the serum in rats fed with or without orotic acid diet were analyzed. In all the hepatic lipid fractions of rats fed on the supplemented orotic acid diet, there was a significant increase in linoleic acid. In addition, linoleic acid was also increased in the triacylglycerol faction of hepatic endoplasmic reticulum and the triacylglycerol and diacylglycerol fractions of hepatic Golgi apparatus of the orotic acid-feeding rats. In the time course study of the fatty acid profile in the hepatic triacylglycerol and diacylglycerol fractions, an increase of linoleic acid was observed similarly in the initial stages of orotic acid intake in the both fractions. However, linoleic acid in the serum triacylglycerol fraction of orotic acid-feeding rats increased from day 1, but it began to decrease the increment from day 2, resulting in the lower level of linoleic acid in the serum triacylglycerol fraction of orotic acid-feeding rats than that of rat fed a orotic acid-free diet after 10 days. Oleic acid (18:1) was increased in the only cholesteryl ester fraction of hepatic. However, oleic acid level in other fractions was not changed. The compositions of 14:0, 16:0 and 18:0 was reduced in the hepatic triacylglycerol, diacylglycerol and cholesteryl ester fractions by orotic acid-feeding. However, these saturated fatty acids were significantly increased in the serum triacylglycerol fraction. The orotic acid induced changes in linoleic acid level in hepatic triacylglycerol may be explained by the impaired fatty acid metabolism and limited excretion of this fatty acid from liver to serum.

Bacillus sp. FF-7에 의한 항산화물질 생산조건과 항산화 활성

차재영,김효정,전방실,박진철,옥민,조영수 한국응용생명화학회 2003 Applied Biological Chemistry (Appl Biol Chem) Vol.46 No.3

발효식품 시료로부터 분리된 세균을 DPPH(α,α¹-diphenyl-β-picrylhydrazyl) 전자공여능으로 항산화 활성을 측정하여 가장 활성이 강한 균주를 선별하여 형태학적, 생화학적, 생리학적 특성 및 16S rRNA 염기서열을 조사한 결과 Bacillus sp.으로 판명되어 FF-7로 명명하였다. DPPH 전자공여능법에 의한 Bacillus sp. FF-7이 생산하는 항산화물질의 최적 생산 배지조건은 탄소원 2% galactose와 질소원 1% tryptone 첨가였다. Bacillus sp. FF-7에 의해 생성된 항산화 물질의 활성을 DPPH 전자공여능, 흰쥐 각 조직 microsomal 실험계 및 linoleic acid 과 산화지질 실험계에서 malondialdehyde를 thiobarbituric acid(TBA)방법으로 측정하였다. 흰쥐 각 조직 microsomal 실험계에서 지질과산화에 대한 항산화 효과는 뇌(97.50%)>심장(79.95%)>신장(77.84%)>비장(77.47%)>고환(69.96%)>간장(62.45%) 순이였다. Linoleic acid의 과산화지질률 TBA법으로 측정한 결과 반응 4일째까지 억제 효과가 강하게 나타났으며, 동시에 대조구로 사용한 0.05% BHT 첨가구에서도 실험종료시까지 항산화 활성이 강하게 나타났다. The antioxidative activity of antioxidative substances produced from several bacterial strains isolated from fermented foods were tested by DPPH (α,α'-diphenyl-β-picrylhydrazyl) free radical scavenging activity. One of the strains showing the highest antioxidative activity was identified as Bacillus sp. based on the morphological, biochemical, physiological characteristics, and 165 rRNA sequence, and named FF-7. The molt optimal medium condition for the production of antioxidative substance from Bacillus sp. FF-7 was 2% galactose as carbon source and 1% tryptone as nitrogen source. The antioxidative substance produced from FF-7 in these cultural medium was also tested by in vitro experimental models, the peroxidation of linoleic acid and the peroxidation of rat tissues microsomes by using thiobarbituric acid (TBA) for assay of free rnalondialdehyde production. The antioxidative avtivity against lipid peroxidation of rat tissues microsomes was shown in the following order; brain 97.50% > heart 79.95% > kidney 77.84% > spleen 77.47% > testis 69.96% > liver 62.45%. The antioxidative substance produced from FF-7 on linoleic acid peroxidation by TBA method was effectively inhibited during four days, and 0.05% BHT(butylated hydroxytoluene) used comparative control was also effectively inhibited. Results showed that the highest antioxidative activity by DPPH method of antioxidative substance produced from Bacillus sp. FF-7 was obtained by supplementing 2% galactose as carbon source and 1% tryptone as nitrogen source in cultured medium, this substance effectively inhibited the formation of TBARS in brain microsome in vitro system and in linoleic acid peroxidation.

흰쥐의 원위콩팥단위내 칼슘결합단백질 (calbindin-D28k)에 관한 면역세포화학적 연구

김영희,김완영,김인범,한승호,오수자,차정호,김진 대한해부학회 1997 Anatomy & Cell Biology Vol.30 No.6

소아 급성 감염성 질환에 대한 Cefoxitin sodium의 치료 효과

차상원,김용주,변상현,이건수,정용헌 최신의학사 1991 最新醫學 Vol.34 No.12

만성두통 환자의 성격유형 A 행태, 스트레스, 우울 및 두통영향정도의 관계 연구

차남현,임사비나,정인태,김수영,안경애,김건식,이재동,이상훈,최도영,이윤호,이두익 EAST-WEST MEDICAL RESEARCH INSTITUTE KYUNG HEE UNI 2005 東西醫學硏究所 論文集 Vol.2005 No.-

To examine an estimate factor and grasp the relation of difference for Ype A Behavior Pattern(TABP), Perceived Stress Questionnaire. Depression and HIT-6 in the Chronic headache client. Method: Data collected by self-reported questionnaires from 38 client in S city who were selected by criteria of IHS, from the 19^(th) of October to 10^(th) of December, 2004. Result: 1) Differences between biographical data by TABP was significant by SaSang constitutions, by Stress was significantly influenced by age, and by Depression were significantly influenced health status and SaSang constitutions. 2) Correlations Coefficients among Study Variables were Stress and Depression(r=.494, p=.002) and Depression and HIT-6(r=.432, p=.010). 3) In regression analysis, HIT-6 were significantly influenced by Depression and Type A Behavior Pattern(TABP). These variables explained 38% and 34% respectively. Conclusion: The result suggest that chronic headache management with psychological aspect, as well as physical aspect should be a focus to enhance the quality of life.

Orotic acid 유발 지방간 rat와 mouse의 중성지질 대사

車載英,조영수 東亞大學校附設遺傳工學硏究所 1997 遺傳工學硏究 Vol.- No.4

Orotic acid 1% 투여에 의해 유발된 脂防肝 SD-계 rat와 kud : ddY mouse의 중성지질대사에 미치는 n-6계 지방산의 효과에 대하여 검토하였다. 그 결과 rat에 orotic acid무첨가 식이와 비교할때 1% orortic acid및 n-6계 PUFA(linoleic acid)가 함유된 반합성 식이 투여군에서 간장의 triglyceride함량은 유의적으로 증가하였고, 반면 혈청 triglyceride 함량은 감소하였다.이에 비해, mouse는 orotic acid 1% 함유식이 투여에 의해 간장triglyceride함량은 rat와 달리 증가하는 경향이었다. 중성지질 최종제한 단계 효소인 phosphatic acid phosphohydrolase의 활성은 triglyceride합성을 촉진시켰으며, orotic acid첨가 식이를 투여한 rat의 간장 cytosol및 microsome에서도 같은 경향이었다. 이러한 것으로부터 이 효소의 활성은 rat에 있어서 지방간 항진에 관여한다는 것이 강하게 시사되었다. Effects of 1% dietary orotic acid on triglyceride metabolism were examined in SD-rats and Kud : ddY mice. When rats were fed semisynthetic diet containing 1% orotic acid and n-6 polyunsaturated fatty acid(linoleic acid), the hepatic triglyceride content was increased significantly by about 3-fold and the serum triglyceride content was decreased compared with orotic acid-free diet. In contrast to rats which respond to orotic and consumption with increases in hepatic triglyceride content, mice did not so respond. The rats-limiting step in triglyceride synthesis is catalyzed by the enzyme phosphatic acid phosphohydrolase (EC 3.1.3.4) which is present in the liver cytosol and microsomes of rate fed orotic acid diet. This finding suggests that the activity of this enzyme may play a role in the fatty liver formation in rats.

병·의원에서 분리한 메티실린 내성 황색포도알균에서 SCCmec 유형과 Aminoglycoside 변형효소 유전자의 분포

정영희,김광욱,차정옥,이경민,유재일,유정식,김봉수,노영주,윤혜령,이영선 대한감염학회 2008 감염과 화학요법 Vol.40 No.1

목적 : 대부분의 AME 효소 유전자는 transposon이나 plasmid 상태로 전달되며 staphylococcal chromosomal cassette mec (SCCmec) 내에도 삽입되어 있다. 최근 MRSA의 SCCmec 유형에 따라 다양한 내성유전자를 가지는 것으로 보고되고 있다. 따라서 1, 2차 의료기관에서 분리된 황색포도알균의 항생제 내성률을 조사하고 MRSA에서 SCCmec type에 따른 AME 효소 유전자의 분포를 확인하였다. 재료 및 방법 : 2004년 1, 2차 의료기관에서 분리된 황색포도알균 640주를 대상으로 methicillin의 내성유전자 mecA와 AME 효소 유전자 aac(6')-aph(2"), aph(3')-IIIa, ant(4')-Ia 등을 multiplex PCR 방법을 이용하여 확인하였다. 항생제 감수성 시험과 MIC 값은 한천배지 희석법으로 확인하였고 SCCmec type도 결정하였다. 결과 : 640주의 황색포도알균 중 MRSA을은 39.7%였으며 MRSA 분리주 모두 mecA 유전자가 검출되었다. Aminoglycoside 중 kanamycin, tobramycin에 대한 내성률은 98.1%이었고, gentamicin 68.7%, amikacin 30.8%, netilomicin 2.8%이었으며 vancomycm에는 모두 감수성이었다. Oxacillin MIC_50과 MIC_90은 각각 128 ug/mL, 256 ug/mL이었으며 254주의 MRSA 중 214주를 선별하여 AME 효소 유전자를 확인한 결과 aph(3')-IIIa 13.1%, aad(6')-aph(2") 77.1%, ant(4)-Ia 53.3%이었으며 SCCmec type에 따라서는 type II, type III, type IV가 각각 50.9%, 16.4%, 32.7%였다. SCCmec type에 따른 AME 효소 유전자의 분포는 SCCmec type II에서는 aac(6')-aph(2")와 aac(6')-aph(2")/ant(4')-Ia가 각각 49.5%, 36.7%에서 검출되었고 type III에서는 aph(3')-IIIa/aac(6')-aph(2")가 60%, aac(6')-aph(2")가 31.4%, type IV에서는 aac(6')-aph(2")/ant(4')-Ia와 ant(4')-Ia가 각각 41.4%, 50%로 나타났다. 결론 : 1, 2차 의료기관에서 분리한 황색포도알균 분리주의 메티실린 내성률은 39.7%이었다. MRSA 분리주 중 SCCmec type II와 III에서는 aac(6')-aph(2")가, SCCmec type IV에서는 ant(4')-Ia가 90% 이상 검출되어 SCCmec type과 AME 효소 유전자 분포와는 연관성이 있는 것으로 나타났다. Background : Many genes encoding aminoglycoside modifying enzymes (AMEs) on transposon or plasmid were transferred from one strain to another strain and inserted into a staphylococcal chromosomal cassette mec (SCCmec). There are very diverse subtypes in SCCmec type to the insertion of resistant genes. Therefore, we researched the resistance rates of antibiotics and distribution of AME genes according to SCCmec type in MRSA strains. Materials and Methods : We isolated 640 Staphylococcus aureus from non-tertiary hospitals in 2004, detected mecA, aac(6')-aph(2"), aph(3')-llla, and ant(4')-la using the multiplex PCR method, tested antibacterial susceptibility disk diffusion and minimal inhibitory concentration, and determined SCCmec type. Results : Of 640 S. aureus isolates, MRSA rate was 39.7% and all MRSA isolates carried mecA gene. Among 214 MRSA selected, aminoglycoside-resistant rates were 98.1% in kanamycin and tobramycin, 68.7% in gentamicin, 30.8% in amikacin, and 2.8% in netilmicin. The detection rates for aac(6')-aph(2"), aph(3')-llla, and ant(4')-la were 77.1%, 13.1%, and 53.3%, respectively. Also, SCCmec type was 50.9% in SCCmec type II, 16.4% in type Ill, and 32.7% in type IV. The genes encoding AMEs were distributed aac(6')-aph(2") (49.5%) and aac(6')-aph(2")/ant(4')-la (36.7%) in SCCmec type II, aph(3')-llla/aaac(6')-aph(2") (60%) and aac(6')-aph(2") (31.4%) in type III, and aac(6')-aph(2")/ant(4')-la (41.4%) and ant(4')-la (50%) in type IV. Conclusion : 39.7% of S. aureus isolated from non-tertiary hospitals was resistant to methicillin. More than 90% of MRSA isolates were detected aac(6')-aph(2") in SCCmec type II and Ill, and ant(4')-la in type IV. With these results, the genes encoding AMEs may be closed related to SCCmec type.