한국인 심부전증 환자 심근에서의 인형 거대 세포 바이러스 감염

이명용,이무용,김영권,한성식,최성준,김효수,이영우,서정돈 단국대학교 1998 論文集 Vol.33 No.-

Objectives: In order to evaluated the prevalence and the site of infection of cytomegalovirus in terminally failing heart, cytomegaloviral DNA was detected in the explanted hearts of transplantation recipients. Methods: DNA extractions were performed from explanted failing hearts(N=22) and normal hearts(N=5) and polymerase cain reactions(PCRs) were done for detection of late gene sequence coding pp150 glycoprotein. The products were confirmed by electrophoresis on the 1% agarose gel. n order to improve the detectability of cytomegaloviral genome, nested PCRs were executed with the primers designed for the original 607 bp products. In situ PCRs also were done with the samples which were confirmed as positive for CMV viral genome by nested PCRs. Results: All patients had IgG anti-cytomegalovirus antibody and did not have IgM anti-cytomegalovirus antibody. Cytomegaloviral genomes in myocardium were detected by polymerase chain reaction. The 607bp products by PCRs were found in both explanted failing heart(3 cases/22. 13.5%) and normal hearts( 1 case/5, 20.0%). In nested PCRs, 186bp products were found in both failing hearts(LV 4/22, LA 3/2, RV 4/22, RA 0/17) and normal hearts(LV 2/5, LA 1/4, RV 2/5, RA 2/5). These was no significant change in positivity of cytomegaloviral DNA genome between failing and normal hearts. Total positivity of cytomegaloviral genome in explanted hearts was 44.4% according to the nested PCR results. The positivity of cytomegaloviral DNA by n situ PCR was 33.3%(4/12), and the site of positive reaction was the nuclei of the myocardial cells. Conclusion: Cytomegalovirus was rarely observed in explanted hearts of terminal heart failure and nested PCR could enhance the sensitivity of cytomegaloviral genome detection. The result of the in situ PCR showed the site of the cytomegaloviral infection was nuclei of the myocardial cells. Cytomegalovirus, however, might have no direct causal relationship in the development of terminal heart failure.

가토 외고정 모델을 이용한 치밀체형 세라믹스의 골결합능 평가

이재협,이동호,이재학,공현식,안영준,류현승,서준혁,홍국선,김환,장봉순,이춘기 대한정형외과연구학회 2003 대한정형외과연구학회지 Vol.6 No.2

소수성 물질을 포함한 커들란 겔의 특성 및 식품으로의 활용 연구

최춘순,이기영,이창문,정제명,이인영 전남대학교 촉매연구소 2001 觸媒硏究 論文集 Vol.22 No.-

The thermal stability of curdlan suspension was investigated. Also curdlan gel containing hydrophobic materials (DHA(Docosa hexaenoic acid), olive oil and Dong-chunghacho (Militaris)) were prepared. Curdlan suspension prepared under 40℃ and 15000rpm was stable for 1 hour. Curdlan gel, including hydrophobic material were dried at 30℃ and swollen in the water. Curdlan gel including Dongchunghacho absorbed water of 980%.

영구형 항서맥 심박조율기 환자의 장기추적관찰

최현석,이무용,최영진,최성준,김효수,손대원,오병희,이명묵,박영배,최윤식,이영우,이명용 대한순환기학회 1998 Korean Circulation Journal Vol.28 No.5

노화가 인체 중간엽 줄기세포로부터 조골세포로의 증식 및 분화에 미치는 영향

백기현,태현정,오기원,이원영,조정기,권순용,강무일,차봉연,이광우,손호영,강성구,김춘추 대한내분비학회 2003 Endocrinology and metabolism Vol.18 No.3

연구배경: 일반적으로 골다공증과 연관된 위험인자로는 연령, 폐경, 약물, 불충분한 칼슘섭취, 만성질환 및 운동부족 등이 있는데, 특히 노화가 진행할수록 골밀도가 감소하는 것은 잘 알려져 있다. 노화와 관련하여 진행되는 골소실은 조골세포 및 전구조골세포의 기능적 결핍에 의한 골형성의 감소가 주요한 요인으로 여겨지고 있다. 그 동안 연령이 조골모 세포의 양과 조골모 세포로부터 성숙조골세포로의 분화 및 증식에 미치는 영향에 대한 일부 보고들이 있었으나 아직 일치된 견해는 없는 형편이다. 방법: 다양한 연령의 사람으로부터 골수를 채취, 중간엽 줄기세포가 포함된 단핵세포를 분리한 후 조골세포로 분화하기 좋은 조건하에서 배양하였다. 대상군은다시 젊은군과 노령군으로 구분하여 다양한 변수를 비교 분석하였다. 일차배양에서는 CFU-F를 계수하여 골수내 중간엽 줄기세포의 수를 추산하였고, 칼슘측정을 통하여 기질의 무기화 정도를 비교하였다. 계대배양후 이차배양에서는 시기별로 알카리성 포스파타제 활성도를 측정하고 오스테오칼신 mRNA의 발현을 관찰하여 젊은군과 노령군 사이의 증식능 차이를 비교하였다. 또한 이차배양 시기별로 MTT 측정을 하여 양군간에 증식능 차이가 있는지 알아보았다. 결과: 1. 일차배양 15일째에 평균 CFU-F의 수는 젊은군에서 유의하게 많았다(젊은군 148.3±28.9, 노령군 54.3±9.1, p=0.02). CFU-F의 평균면적은 젊은군에서 넓은 경향을 보였으나 통계적으로 유의하지는 않았다. 2. 일차배양 17일 경과 후 양군간에 기질 칼슘 침착정도는 유의한 차이를 보이지 않았다(젊은군 103.6±50.6, 노령군: 114.0±56.5, p=NS). 3. 이차배양 10일째에 젊은군에서 알카리성 포스파타제 활성도가 고령군에 비해 유의하게 높았다(젊은군: 935.5±115.0 U/mg, 노령군: 578.4±115.7U/mg,p.0.05). 고령군에서는 시간 경과에 따른 변화가 미약했으며 전반적으로 알카리성 포스파타제의 활성도가 젊은군에 비해 낮았다. 4. 이차배양도중 오스테오칼신 mRNA의 발현은 배양시기별로 젊은군에 비해 고령군에서 더 낮은 경향을 관찰할 수 있었다. 5. 이차배양 10일과 15일에 젊은군에서 노령군보다세포증식이 유의하게 증가된 양상을 보였다(10앓 젊은군 0.73±0.05, 노령군 0.58±0.04, p=0.05, 15일; 젊은군 0.80±0.05, 노령군 0.70±0.03, p=0.05).결론: 이상의 연구에서 저자들은 노령군에서 젊은군보다 골수 내 중간엽줄기세포의 수가 적고, 노령군에서 유래한 전구조골세포의 성숙조골세포로의 증식 및 분화가 젊은군 보다 감소해 있는 것을 관찰할 수 있었다. Background: Osteoblasts originate from osteoprogenitor cells in bone marrow stroma, termed mesenchymal stem cells (MSCs) or bone marrow stromal cells. Each MSC forms colonies (colony forming units-fibroblasts [CFL-Fs]) when cultured ex vivo. There are some reports about the age-related changes of the number and osteogenic potential of osteoprogenitor cells, but any relationship has not been clearly established in humans. In this study, we counted MSCs using CFU-Fs count and examined the proliferative capacity and differentiation potential of osteoprogenitor cells. Finally, we analyzed how these parameters varied with donor age. Methods: Bone marrow was obtained from the iliac crest of young (n=6, 27.2±8.6 years old) and old (n= 10, 57.4k6.7 years old) healthy donors. Mononuclear cells, including MSCs, were isolated and cultured in osteogenic medium. In primary culture, we compared the colony-forming efficiency of MSCs between the two groups and determined the matrix calcification. When primary culture showed near confluence, the cells were subcultured. Alkaline phosphatase activity, osteocalcin expression by RT-PCR and proliferative potential by MTT assay were examined by the time course of secondary culture. Results: At the 15th day of primary culture, the mean number of CFU-Fs was significantly higher in the younger donors (young: 148.3±28.9, old: 54.3±9.1, p=0.02) and the mean size of CFL-Fs was also larger in the younger donors than the older donors. However, matrix calcification was not different between the two groups (young: 103.6±50.6, old: 114.0±56.5, p=NS). In secondary culture, alkaline phosphatase activities were significantly lower in the older donors. The younger donors showed peak alkaline phosphatase activity at day 10, while the older donors didn't showed a remarkable peak (young: 935.5±115.OU/mg, old: 578.4±115.7U/mg, p<0.05). Total cell number as a proliferative index increased progressively during the secondary culture and a significantly greater cell number was noted in the younger donors. Osteocalcin expression was generally upregulated in the younger donors, but this was not statistically significant. Conclusion: Our study shows that the number of osteoprogenitor cells is decreased during aging and that the proliferative capacity and differentiation potential of osteoprogenitor cells seem to be reduced during aging (J Kor SOC Endocrinol 18:296-305, 2003).

Junctional Rhythm을 보인 한국형 출혈열 1례

이성원,이수훈,최대현,손영기,차광수,손춘희,정원태,김성은,김종성 동아대학교의과대학 1997 동아의대학술지 Vol.9 No.1

障碍領域別 特殊學敎 敎師의 스트레스 比較

李相春,吳永愛 大邱大學校再活科學硏究所 1986 再活科學硏究 Vol.7 No.1

The purpose of this survey is to grasp the Korean special school teachers' stress degrees in the real educational fields, and to compare the different stress degrees by the teaching responsibilities, by sex, by age, by the careers of special teaching profession, by his or her assiduity, by the academic careers and teacher-qualification, and by the reasons of stress. To perform this survey, the writer analysed the answer sheets of the questionnairs returned by 766 special school teachers throughout the country. The results of the analysis are as follows: First, the mean value of stress by the whole teachers was shown as endurable, and there was no difference among stress levels by the teaching responsibilities. As for the ratio of teachers confronted with the dangerous level of stress, the teachers taking charge of mentally retarded students occupied the highest ratio, and those charging of visually handicapped students, the lowest. Second, the comparison by age didn't result in any difference, but in the relation of age to the teaching responsibilities there appeared meaningful differences. That is in the case of the teachers charging of mentally retarded students, it revealed that the older they were, the more stress they suffered. In the case of the teachers charging of visually handicapped students, the tendency was opposite. The comparison by the special teaching profession careers, there showed no difference in the- stress degree. The comparision by theachers' assiduity showed that the stress by the teachers having the intention to stay in the job for the time being was much higher than that by the teachers who had made up their minds to think of their job as a permanent one. The high or low academic careers didn't make any influence on the teachers' stress degree, but the teacher-qualifications made a meaningful difference on their stress degree. That is, the teachers having the special teacher-qualification turned out being suffered much more stress than those having the general teacher-qualification. Third, the comparison of stress degree by the reasons revealed that the most severe degree of stress came from the relations with the parents as well as students' behaviors. The teachers in charge of mentally retarded and physically handicapped students had highest degree of stress because of students' behaviors, while the teachers taking care of visually and auditory handicapped students had the highist degree of stress because of the relation with the students' parents.

MQL 선삭가공시 가공조건의 표면거칠기 영향 분석

이춘만,황영국,정종윤 한국공작기계학회 2004 한국공작기계학회 추계학술대회논문집 Vol.2004 No.-

At present, industry and researchers are looking for ways to reduce the use of lubricants because of ecological and economical reasons. Therefore, metal cutting is to move toward dry cutting or semi-dry cutting. This paper presents an investigation into MQL(Minimum Quantity Lubrication) machining with the objective of deriving the optimum cutting conditions for the turning process of SM45C. To reach these goals several finish turning experiments were carried out, varying cutting speed, feed rate and oil quantity, with MQL. The surface roughness results of tests were measured and the effects of cutting conditions were analyzed by the method of Analysis of Variance(ANOVA). From the experimental results and ANOVA, it is found that a better surface roughness can be obtained by decreasing oil quantity and feed rate.

배추 Plug育苗條件이 苗素質에 미치는 影響

李喆浩,崔周鎬,朴重春,孫嶺杰,朴鳳植,李炯貞 진주산업대학교 농업기술연구소 1999 農業技術硏究所報 Vol.12 No.-

배추 plug육묘 조건을 알아보기 위하여 상토 종류, 질소시비수준, plug트레이 배수공수준을 달리하여 실험한 결과를 요약하면 다음과 같다. 1. 유묘출현율은 A상토<B상토<C상토의 順으로 높았고, 질소시비수준은 무비구에서 높고, 시비구에서는 90∼90ppm수준에서 높은 편이었으며 배수공수에 따라서는 배수공이 적은 128공구가 출현율이 높았다. 2. 幼苗의 葉발생도 A상토<B상토<C상토 순으로 높았고, 질소시비수준은 다비구(150ppm구)에서 엽수 5배로 최대엽수를 나타내었고, 배수공수에 따라서는 배수공이 적은 128공구가 높았다. 3. 엽장의 길이는 A상토구는 128공구에서 질소시비수준 120ppm에서 6.98cm로 가장 길었으며, B상토구에서 128배수공구의 시비수준 150ppm에서 12.41cm로 가장 길었고, C상토구에서도 128배수공구의 시비수준 150ppm에서 12.88cm, 200배수공구에서도 9.29cm로 가장 길었고 무비구에서는 공히 가장 짧았다. 4. 葉幅도 엽수와 엽장과 비슷한 경향으로 기비로 사용된 질소의 시비수준이 증가할수록 엽장이나 엽폭이 증가하여 B상토구의 150ppm 시비구에서 4.80cm로 최대치를 나타냈으며 시비량이 감소할수록 엽폭도 줄어드는 경향이였다. 5. 뿌리의 신장은 A상토구의 128배수공구에서 질소질 함량이 90∼120ppm수준의 범위내에서 신장이 양호하였으나 150ppm시비구에서는 불량한 반면, B와 C상토구에서는 90∼150ppm 모두에서 양호하였다. 6. 생체중과 건물중도 상토조성, 질소질비료 및 트레이 크기에 따라 모두 크게 영향을 받는 것으로 나타났으며 C상토구의 150ppm시비구에서 주당 3.0g의 생체중에서 최대치를 보였고 건물중 역시 C상토구의 150ppm시비구에서 4주에 600mg의 생체중의 증가를 보여 최대의 기록을 나타냈고 질소질의 농도 150ppm이 생체중 및 건물중 증가에 가장 효과적인 것으로 나타났다. The experiments were conducted to develop ideal substrates for the production of good quality in cabbage plug and also to improve the media with low or non environmental pollution by blending and mixing artificial substrate including peat moss, coir, perlite, vermiculite, carbonized rice hull and zeolite. In effects of soil substrate combination, nitrogen concentration and tray cell size on growing of 'Baekro', chinese cabbage, the ratio of seedling emergency in all fertilizer block was higher than non-fertilizer of nitrogen by 80%. The number of leaves were most increased in the 150ppm nitrogen solution of B block(coir 50%, vermiculite 10% and perlite+carbonized rice hull-zeolite 40%) and C block(coir 40%, vermiculite 20% and perlite+carbonized rice hull+zeolite 40%). The leaf length and leaf width were similar with that result in the number of leaves. Rooting condition was the best in the 90∼120ppm nitrogen solution of A block(TOSILI No1, SHINAN-JEONGMIL(Co)) in 128 tray cell size. The fresh weight and dry weight were increased as the order A<B<C in soil combination, were increased in the fertilizer which has more nitrogen concentration and were increased higher in 128 than 200 tray cell size, and chlorophyll concentration was the highest in the 150ppm nitrogen solution of A and B soil combination.

혈액투석 환자에서 대퇴신경범증으로 발현된 장골근 및 요근 농양 1예

이준상,강동구,신상열,윤재호,조영일,고성은,유영범,전혜정,진춘조,송종오 대한신장학회 2004 대한신장학회잡지 Vol.23 No.1