가감청영탕(加減淸營湯)에 효과를 보인 열성 아토피 피부염 환자 6례 증례 보고

서지혜,정창환,박선정,임소영,한수련,Seo, Ji-Hye,Jung, Chang?Hwan,Park, Seon-Jeong,Lim, So-Young,Han, Su-Ryun 대한한방안이비인후피부과학회 2014 한방안이비인후피부과학회지 Vol.27 No.3

Objective : The purpose of this study is to investigate the effect of Gagamchengyoung-tang($Ji\bar{a}ji\check{a}nq\bar{i}ngy\acute{i}ng-t\bar{a}ng$) for atopic dermatitis patients diagnosed as the febrile tendency. Method : This case study was done on 6 atopic patients with febrile tendency who have visited Korean Medicine Clinic from 2013.08.01. to 2014.04.30.. Gagamchengyoung-tang($Ji\bar{a}ji\check{a}nq\bar{i}ngy\acute{i}ng-t\bar{a}ng$) was prescribed to all 6 patients and evaluated the symptom change through photographs and questionnaire. Objective SCORAD Index(OSI) was used to evaluate the objective symptom and VAS was used to evaluate the subjective change. Results & Conclusion : The Objective SCORAD index and VAS were decreased in all patients. The average OSI reduction score was 26.3. Most symptoms of 6 patients were improved. Gagamchengyoung-tang ($Ji\bar{a}ji\check{a}nq\bar{i}ngy\acute{i}ng-t\bar{a}ng$) can be effective in patients who were diagnosed as febrile tendency.

커뮤니티공간으로서의 어린이공원 조성에 관한 연구

김연금,김성주,박환지,유다희,최성용,홍승모,Kim, Yun-Geum,Kim, Seong-Joo,Park, Hwan-Ji,Yu, Da-Hee,Choi, Sung-Young,Hong, Seung-Mo 한국조경학회 2008 韓國造景學會誌 Vol.35 No.6

This study deals with the participatory design process of renovating a children's park. The process should proceed with two major elements. Firstly, the children's park should be designed from the perspective of children. Secondly, it should be designed in consideration of the context of the community. This study is divided into two parts. First is the review of theory concerning the meaning and roles of the children's park as a community space, the importance of children's participation in the design and the participatory method of the children. The second is the case study. The site for the case study is Ssial Park in Nowon-Gu, Seoul. The entire process of the case study is with community participation, from identifying the park's problems to the actual construction. At every step, through the participatory programs, the community participates in the decision making. In addition, mutual understanding among participants is pursued through diverse means of communication. The following results of the case study were found. Firstly, diverse participatory programs and the active trial to enable the community to communicate contribute to the sense of ownership and responsibility concerning the park Secondly, the community can negotiate the differences in opinions without needing the help of experts. Of course, there are limits in the case study, such as the fact that the community organization, which is the core of communication and maintenance concerning the park, is not organized through the process of a case study. Another is that more diverse methods, which inform all community members of the participatory process, should have been used.

Multiplex PCR Detection for 3 Events of Genetically Modified Maize, DAS-59122-7, TC6275, and MIR604

Ji-Hye Ahn,Jae-Hwan Kim,Su-Youn Kim,Woo-Young Lee,Sun-Hee Park,Hae-Yeong Kim 한국식품과학회 2008 Food Science and Biotechnology Vol.17 No.3

A multiplex polymerase chain reaction (PCR) method was developed to simultaneously detect 3 events of genetically modified (GM) maize. The event-specific primers were used to discriminate the following 3 events of GM maize (DAS-59122-7, TC6275, and MIR604) using multiplex PCR method. The zein gene was used as an endogenous maize reference gene in the multiplex PCR detection. The primer pair Zein-F/R producing a 99 bp amplicon was used to amplify the zein gene. The primer JI-Das-F1/R1 for DAS-59122-7, JI-TC6275-F3/R3 for TC6275, and JI-MIR F1/R1 for MIR604 yielded an amplicon of 130, 162, and 197 bp, respectively. The detection limit of multiplex PCR was 1% for DAS-59122-7, TC6275, and MIR604 for one reaction.

Slide Session : OS-IFD-07 ; Infectious Disease : In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus

( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.

MOS varactor를 이용한 저 위상잡음 CMOS LC VCO 설계

박재홍,여지환 대구대학교 정보통신연구소 2003 情報通信硏究 Vol.1 No.2

본 논문에서 전압제어 발진기는 2.4㎓에서 동작하도록 설계하였다. 설계된 전압제어발진기는 저 전력, 집적화를 위하여 0.25um 표준 CMOS공정을 이용하여 CMOS differential oscillator 형태, 공진부에는 MOS varactor를 이용하여 설계했으며, HP사의 ADS를 이용하여 검증하였다. 전원전압 2.5V, 전류 3㎃에서 동작되며 제어전압이 -0.3V∼1.6V일때 주파수는 2.33㎓∼2.56㎓까지의 동작범위를 가진다. 위상잡음은 전체가변 범위에서 600㎑ offset일 때 -118.3 ㏈c/㎐∼-126.5 ㏈c/㎐ 사이의 값을 가진다. In this paper, a voltage-controlled oscillator have been designed to operate on the 2.4㎓ band. A designed of voltage-controlled oscillator is for suitable low power and integrated circuits using a standard 0.25-um CMOS process. Resonator circuit is made by accumulation mode varactors and simulated using software ADS in HP. In result operating voltage is 2.5V and current is 3㎃. Controled voltage is -0.3V∼1.6V and the range of frequency is from 2.33㎓ to 2.56㎓. The phase noise is measured at 600 ㎑ offset and is from -118.3 ㏈c/㎐ to -126.5 ㏈c/㎐.

베타-아밀로이드 펩타이드로 유발된 신경세포사와 tau 인산화와의 연관성

김지형,홍혜남,이종환,황승준,박형섭,이흥식,김동호 대한해부학회 1998 Anatomy & Cell Biology Vol.31 No.4

한국산 자리공 자리공 열매의 적색소를 이용한 식용색소 : Ⅱ. 활성 사포닌을 제거한 색소의 안정성과 가공적성 Ⅱ. Color Stability and Characteristics as Food Colorant of Saponin-Free Pokeberry Pigments

박용석,서지연,황혜정,박기환,윤광로 中央大學校 食糧資源硏究所 2003 食糧資源硏究所 論文集 Vol.15 No.1

우리나라산 자리공 열매의 색소 함량은 산지에 따라 건물 g당 48.56에서 62.16mg으로 천연색소 자원으로 높은 편이였으며 산지간의 격차가 크지 않아 자원성이 좋은 편이었ㄷ. 자리공 열매의 적색소를 absolute ethanol 침전법으로 분획하는 과정에서 활성 사포닌이 95% 이상 제거되며 이 색고 침전물을 silica gel column이나 Sephadex G-25 column으로 정제함으로서 색소의 순도를 높일 수 있었다. Absolute ethanol 처리와 column 정제법을 응용하여 활성 사포닌을 제거 한 실험용 자리공 열매색소 분획 모두 색소 안정성이나 가공적성에서 뚜렷한 차리를 보이지 않았다. 따라서 absolute ethanol만 처리한 색소제품이나 이를 column chromatography로 정제한 색소분획 모두 고순도의 천연색소 첨가물로의 응용 가능성은 분명하였다. 이들 활성 사포닌을 제거한 자리공 열매색소의 색소안정성과 가공적성은 다음과 같이 요약 할 수 있다. 즉, pH 안정범위는 4.8-6.0이었으며, 열에 대한 안정성을 조사한 결과는 4℃에서 45일 저장했을 때는 70%의 색소 잔존율을 나타낼 정도로 안정한 편이었지만 25℃에서는 10일째에서 70% 잔존율을, 50℃이상의 온도에서는 수 시간 정도에서 50%이하의 색소 잔존율을 보여 전반적으로 열 안정성은 불량한 편이었다. 한편 이들의 존재 하에서는 급격한 변색이 진행되었다. 자리공열매 색소에 대하여 ascorbic acid나 palmityl ascorbate의 첨가는 색소 잔존율에 큰 영향을 미치지 않았으며 chlorogenic acid, resocinol 그리고 catechin의 첨가도 마찬가지였다. 포도당, 과당, 맥아당, 설탕, 펙틴, 셀루로스 그리고 cyclodextrin 등의 탄수화물도 전혀 영양을 미치지 않았다. 1000ppm 이내의 EDTA나 citric acid, malic acid 또는 oxalic acid 등의 산도 미치지 않았다. 금속이온으로서, Ca^(2+)과 Al^(3+)의 첨가는 색소의 잔존율에 큰 영향을 미치지 않았으나, Cu^(2+)나 Fe^(2+)의 첨가는 심한 변색을 초래하였다. 자리공 열매즙의 무수 알코올 처리물(적색 색소 침전물)은 soft gel, firm gel 또는 캔디 등의 고형 식품에 잘 어울리고, 음료에서는 변색이 빠르게 진행되며, 특히 요구르트의 경우가 가장 민감하였다. 결론적으로 보면, 우리나라산 자리공 열매에서 활성 사포닌을 제거하고 확보한 색소제품은 사탕무의 betanin과 거의 같은 색소안정성과 가공적성을 보여 줌으로서 사탕무색소 첨가제인 beet red를 대신할 수 있으며 색소의 함량이나 순도라는 관점에서는 훨씬 우수할 것으로 사료된다. To evaluate the basic data on the color stabilities of saponin-free pokeberry pigment products, % remain of pigment were calculated from absorbances of solutions stored at various conditions. The pigment products were estimated to be very stable at pH 4.8-6.0. Thermostability of pH 5.0 solution of pigment products could be kept stable below 4℃. Addition of Cu2+ or/and Fe2+ decreased the % remain of pigment, whereas most food components had no offset the stability of pokeberry pigment. The color changes of some model foods having pokeberry pigment were checked by Hunter color differerence meter for 45 days. In solid foods like gels and candy the changes of color difference were very slower than that of model beverages. Application of pokeberry pigment to soft drink and yogurt were found to be effective at the refrigerated condition.

편측 협응훈련에 의한 운동신경망의 재조직 및 교차훈련의 효과

박지원,김종만,서정환,김연희 한국전문물리치료학회 2002 한국전문물리치료학회지 Vol.9 No.3

We report the reorganization of motor network resulted from intensive unilateral coordination training and the effect of cross education on the untrained side in patient with traumatic brain injury using functional magnetic resonance imaging (fMRI). A 22 year-old male patient who had suffered from diffuse axonal injury for 58 months showed coordination deficit in the left hand at initial examination. Intensive motor training including complex finger movements and coordination activities using a metronome was introduced to the patient 4 hours per day for a week. FMRI was performed on a 3T ISOL Forte scanner. All functional images were analyzed using SPM-99 software. Hand function was improved after training not only in the trained lefr hand, but also in the untrained right hand. There was no activation in the right primary motor area (M1) during left hand movement before training whereas robust activation of left M1 was demonstrated by the right hand movement. Profuse activation of bilateral prefrontal lobes was seen during both hand movements before training. After training of left hand, right M1 became prominently activated during the left hand motion. The activation of bilateral prefrontal lobes disappeared after training not only for the left hand movement but also for the right, which clearly demonstrated the effect of cross education. This case report demonstrated the learning-dependent reorganization of the M1 and the effect of cross education.

원형관로내 층류 맥동유동의 속도와 압력 분포에 관한 연구

박길문,유영태,위광환,이충주,정지철 조선대학교 생산기술연구소 1996 生産技術硏究 Vol.18 No.2

In this study basic equations for the pulsatile laminar flow in a circular rigid pipe containing a slightly compressible fluid are derived and analytical solutions for the distributions of wave propagation factor K, cross-sectional mean velocity u_(m) and pressure P are schematically developed and confirmed experimentally. The pulsatile flow with 0 ≤ f ≤ 48Hz and 0 ≤ Re ≤ 2500 generated by summing the oscillatory flow made by ball valve into steady one. The wave form of pulsatile flow is described as a longitudinal wave and particle velocities measured experimentally are group velocities of the wave packet which are always smaller than phase velocities which are approximate to the sonic velocity C when the radial dispersion and wall friction are negligible. Therefore wave number is approximated to k = ω/c for high angular velocity region. The axial pressures and centerline velocities were measured by strain-gage type pressure transducers and Pito-static tubes, respectively. The cross-sectional mean velocities calculated from centerline velocities with the parabolic distributions of the laminar flow and confirmed by using the ultrasonic flowmeter. We knew that axial distributions of oscillatory velocity and pressure coincide with thoeretical and depend on the Reynolds number and angular velocity.

졸-겔법으로 합성된 폴리이미드/실리카 복합체의 환경적 물성에 관한 연구

박성수,홍성수,이성환,김성완,박재현,김지경,박희찬 한국환경과학회 2003 한국환경과학회지 Vol.12 No.4

Two types of polyimide/silica composite films were prepared using sol-gel method through hydrolysis and polycondensation of tetraethoxsilane(TEOS) with the polyamic acid(PAA) and end-capped PAA solution. Sample were characterized by the means of differential thermogravimetry, X-ray diffractometry, scanning electron microscopy, universal test, impedance analyzer, chemical resistance test, etc. All of the PAA/silanol solutions heat-treated at 300℃ for 6h were transformed to polyimide/silica composites. It has been demonstrated that the properties of polyimide/silica composites were affected by the amount of silica addition and the bond type existed between polyimide and silica.