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김창이,김수평,이종건,나종구,홍승덕,신종철,김찬주,송경근,조태준 대한산부인과학회 1994 Obstetrics & Gynecology Science Vol.37 No.8
가톨릭의과대학 부속 성모병원에서 1990년 1월 1일부터 1992년 12월 31일 사이에 조기분만한 362명의 신생아(쌍태아 17명)와 345명의 산모의 의무기록을 조사하여 각 임신주수와 혈압, 임신중독증 여부, 24시간 이상된 조기 양막파수, 분만전 출혈여부, 분만전 진통유무, 분만방법, 신생아의 성별, 자궁내 태아의 발육지연 등의 유무에 따른 신생아 호흡곤란 증후군의 발생정도를 Multiple logistic technique을 이용하여 분석 비교한 후에 다음과 같은 결과를 얻었다. 1. 24시간이상 조기양막파수, 그리고 24시간 이상된 질출혈을 조정하였을 때 확장기 혈압이 110이상인 임신성 고혈압군은 정상 혈압군보다 유의하게 높은 신생아 호흡곤란 증후군의 위험도를 나타내었다. 2. 혈압, 24시간이상 조기 양막파수, 24시간 이상 질출혈, 산전분만통의 유무, 신생아의 성별, 자궁내 태아발육지연 등을 조정하였을 때, 임신주수와 분만방법이 신생아 호흡곤란증후군에 영향을 주는 유의한 위험인자로 입증되었다. Hypertension during pregnancy continues to be a major cause of preterm delivery and neonatal morbidity due to RDS. The effect of hypertension in pregnancy on the incidence of neonatal RDS remains controversial. It is due to the fact that the incidence of neonatal RDS was associated with confounding variable such as membrane rupture$gt;24 hours, vaginal bleeding, undergoing labor, having a cesarean section, growth retardation and neonatal sex. In order to investigate the assocation between maternal hypertension and the incidence of neonatal RDS, we used the multiple logistic regression to control for the above mentioned variabled and the 362 preterm birth were retrospectively analyzed. The results were as follows: 1. After adjustment for membrane rupture$gt;24 hours and vaginal bleeding, the risk of developing respiratory distress syndrome was significantly greater(adjusted odds ratio; 2,990, P value=0.002) in babies of hypertensive mothers(diastolic B.P.$gt;110 mmHg). 2. After adjustment for blood pressure, membrane rupture$gt;24 hours, vaginal bleeding, labor, neonatal sex and growth retardation, the risk of developing respiratory distress syndrome was significantly greater (adjusted odds ratio; 1.833, P value=0.04) in babies delivered by cesarean section without labor. These results suggests that the incidence of RDS in babies of hypertensive mothers might be more escalated due to the absence of labor before delivery because of the greater likehood of cesarean section.
RhD 음성 임산부의 말초혈액으로부터 태아 RhD 상태의 산전검색
권용일,김창이,김수평,신종철,이종승,백은정,정대영,노승혜,최영근,홍옥기 대한산부인과학회 1998 Obstetrics & Gynecology Science Vol.41 No.3
Objective: To determine usefulness as a method that allow prenatal diagnosis of fetal RhD status using polymerase chain reaction [PCR] from maternal peripheral blood. Study design: Thirteen maternal peripheral blood samples obtained at 7∼34 weeks` gestation were tested for fetal RhD status using heminested PCR with primers specific for the genes cording for RhD and RhCc/Ee. After first amplification of 30 cycles with primers RD-A3/RD-A2 [291bp in size], second amplification of various cycles was performed using primers RD-A5/RD-A2 [262bp in size]. Paternal and fetal RhD status was confirmed by serologic and/or PCR methods. Results: All fathers and fetuses were typed RhD positive. After first amplification of 30 cylces, fetal RhD status could be determined in one of 13 cases. Fetal RhD status could be determined in 2, 12, 12 and 13 of 13 cases in each after second amplification of 10, 15, 20 and 25 cycles. Conclusion: This study was suggested that a noninvasive method for prenatal diagnosis of the fetal RhD status using heminested PCR offers useful information for the management of Rh-negative pregnant women. Furthermore, this method can be applied to other genetic disorders and is expected to become the preferred method of noninvasive prenatal diagnosis in DNA level.
Ok-In Cho,Antheunis Versluis,Gary SP Cheung,Jung-Hong Ha,Bock Hur,Hyeon-Cheol Kim 大韓齒科保存學會 2013 Restorative Dentistry & Endodontics Vol.38 No.1
Objectives: This study compared the cyclic fatigue resistance of nickel–titanium (NiTi) files obtained in a conventional test using a simulated canal with a newly developed method that allows the application of constant fatigue load conditions. Materials and Methods: ProFile and K3 files of #25/.06, #30/.06, and #40/.04 were selected. Two types of testing devices were built to test their fatigue performance. The first (conventional) device prescribed curvature inside a simulated canal (C-test), the second new device exerted a constant load (L-test) whilst allowing any resulting curvature. Ten new instruments of each size and brand were tested with each device. The files were rotated until fracture and the number of cycles to failure (NCF) was determined. The NCF were subjected to one-way ANOVA and Duncan’s post-hoc test for each method. Spearman’s rank correlation coefficient was computed to examine any association between methods. Results: Spearman’s rank correlation coefficient (p = -0.905) showed a significant negative correlation between methods. Groups with significant difference after the L-test divided into 4 clusters, whilst the C-test gave just 2 clusters. From the L-test, considering the negative correlation of NCF, K3 gave a significantly lower fatigue resistance than ProFile as in the C-test. K3 #30/.06 showed a lower fatigue resistance than K3 #25/.06, which was not found by the C-test. Variation in fatigue test methodology resulted in different cyclic fatigue resistance rankings for various NiTi files. Conclusions: The new methodology standardized the load during fatigue testing, allowing determination fatigue behavior under constant load conditions