Study on the Elastic Characteristics of Living Cells using Atomic Force Microscope Indentation Technique

Kwon Eun-Young,Kim Young-Tae,Kim Dae-Eun Korean Tribology Society 2006 KSTLE International Journal Vol.7 No.1

In this work, imaging and study of elastic property of the living cell was performed. The motivation of this work was to seek the possibility of exploiting Young's modulus as a disease indicator using Atomic Force Microscope (AFM) and also to gain fundamental understanding of cell mechanics for applications in medical nanorobots of the future. L-929 fibroblast adherent cell was used as the sample. Imaging condition in cell culturing media environment was done in very low speed ($20{\mu}m/ s$) compared to that in the ambient environment. For measuring the Young's modulus of the living cell, AFM indentation method was used. From the force-distance curve obtained from the indentation experiment the Young's modulus could be derived using the Hertz model. The Young's modulus of living L-929 fibroblast cell was $1.29{\pm}0.2$ kPa.

Study on the Elastic Characteristics of Living Cells using Atomic Force Microscope Indentation Technique

Eun-Young Kwon,Young-Tae Kim,Dae-Eun Kim 한국트라이볼로지학회 2006 KSTLE International Journal Vol.7 No.1

In this work, imaging and study of elastic property of the living cell was performed. The motivation of this work was to seek the possibility of exploiting Young’s modulus as a disease indicator using Atomic Force Microscope (AFM) and also to gain fundamental understanding of cell mechanics for applications in medical nanorobots of the future. L-929 fibroblast adherent cell was used as the sample. Imaging condition in cell culturing media environment was done in very low speed (20 μm/s) compared to that in the ambient environment. For measuring the Young’s modulus of the living cell, AFM indentation method was used. From the force-distance curve obtained from the indentation experiment the Young’s modulus could be derived using the Hertz model. The Young’s modulus of living L-929 fibroblast cell was 1.29 ± 0.2 kPa.

AFM indentation 기법을 이용한 세포의 탄성특성에 관한 연구

권은영(Eun-Young Kwon),김영태(Young-Tae Kim),김대은(Dae-Eun Kim) 한국트라이볼로지학회 2006 한국트라이볼로지학회 학술대회 Vol.2006 No.6

In this work, imaging and investigation of elastic property of the living cell was performed. The motivation of this work was to seek the possibility of exploiting Young's modulus as a disease indicator using Atomic Force Microscope (AFM) and also to gain fundamental understanding of cell mechanics for applications in medical nanorobots of the future. L-929 fibroblast adherent cell was used as the sample. Imaging condition in cell culturing media environment was done in very low speed(20 ㎛/s) compare to that in ambient environment. For measuring the Young's modulus of the living cell, AFM indentation method was used. From the force-distance curve obtained from the indentation experiment the Young's modulus could be derived using the Hertz model. The Young's modulus of living L-929 fibroblast cell was 1.29 ± 0.2 kPa.

P087 : Adverse effects of oral cyclosporine in the treatment of various skin diseases

( Hyung Il Kwon ),( Eun Jin Kim ),( Hyun Woo Kim ),( Hyun Chul Park ),( Jeong Eun Kim ),( Joo Yeon Ko ),( Young Suck Ro ) 대한피부과학회 2013 대한피부과학회 학술발표대회집 Vol.65 No.2

Background: Cyclosporine (CS) has been used for various skin diseases including psoriasis and atopic dermatitis. However, dermatologists are reluctant to use CS in clinics because of the adverse effects. Objectives: To investigate the adverse effects of CS for dermatological uses. Methods: A retrospective study of 1,335 patients with CS treatment was performed. Results: 15.6% of patients showed adverse effects. Gastrointestinal (GI) symptoms were the most common adverse effect. Other adverse effects included hypercholesterolemia, hypertrichosis, headache, hypertension, hypertriglyceridemia in order of frequency. Hypercholesterolemia was more often in men, but GI symptoms and hypertrichosis in women. Hypercholesterolemia, hypertriglyceridemia and GI symptoms were more common in adults, but hypertrichosis in young. GI symptoms and headache occurred more often within three months of CS treatment, but hypertension, hypercholesterolemia and hypertriglyceridemia after three months. Hypertrichosis was more often in group with low initial dose. GI symptoms occurred more often with lower CS cumulative dose group and hypertension and hypertriglyceridemia in higher CS cumulative dose group. Conclusion: Comparing to previous studies, the frequency of adverse effects of CS was proven to be low. Therefore, in dermatologic field, we suggest that CS is relatively safe with periodic follow-up.

장기간 항갑상선제 투여 후 임의 중단 뒤 재복용시 발생한 무과립구증 1 예

김승만;김태영;박수영;배영환;서영경 정은주;김태균;권민정;박정현;이순희 인제대학교 2011 仁濟醫學 Vol.32 No.-

Agranulocytosis, one of the major side effects of antithyroid a gents, probably occurs in less than 0.25% of cases treated with antithyroid agents. It usually occurs within the first several weeks or months after the initiation of therapy, when drug dosage is higher. Most reported cases of agranulocytosis have occurred during the first 3 months of therapy. We describe here a rare case that did not experience the side effect for 6 years of antithyroid agent treatment but presented with agranulocytosis in one month after re-exposure. It suggests that the major side effect such as agranulocytosis can occur in patients who had undergone prior uneventful antithyroid drug therapy.

가토의 뇌동맥류 모형에서 모동맥 및 동맥류 협자에 따른 동맥류 내압의 변화 :기초 연구

김은영,송준혁,김명현,박향권,김성학,신규만,박동빈 대한신경외과학회 1999 Journal of Korean neurosurgical society Vol.28 No.4

혈당 측정용 스트립 개발에 관한 연구

권두한,정태화,남효진,송은영,변시명,김희정,김경아,이홍수 THE KOREAN SOCIETY FOR BIOMEDICAL LABORATORY SCIEN 1998 Journal of biomedical laboratory sciences Vol.4 No.2

과산화 효소와 포도당 산화효소의 효소반응을 이용하여 혈액 중 당을 측정하는 혈당측정용 스트립을 개발하였다. 멤브레인에 포도당 산화효소와 과산화 효소, 색원체를 건조 처리하면 혈당이 멤브레인에 처리된 포도당 산화효소와 즉각 반응하여 과산화 수소를 발생하고 발생된 과산화 수소가 과산화 효소와 반응하여 착색물을 형성한다. 제조된 스트립은 혈액과 접촉하면 반응시간 2∼3분 이내에 0∼800mg/dl의 혈당에 대하여 농도에 따라 연 녹, 청녹, 짙은 청색을 나타내며 이때 민감도는 40 mg/dl이었다. 육안용 혈당 스트립을 이용하여 정상을 포함한 당뇨 환자의 혈당 농도를 측정한 결과 Ames사와 BM사에서 시판하고 있는 제품과 유사한 결과를 얻을 수 있었다. 본 연구에서 개발된 스트립이 자동 분석기기의 재료로 활용될 수 있는지 여부를 알아보기 위하여 분광 비색계로 발색 반응의 최적 파장을 분석하고 최적 파장에서 각 혈당 농도별 반사 밀도를 측정하여 검정선을 얻었다. 이 검정선에 의해 임상 혈청시료의 혈당 농도를 측정한 결과 일본의 Kyoto Daiichi사의 혈액 분석시스템과 유사한 결과를 얻었다. 이로서 본 연구에서 개발한 혈당 스트립을 이용하여 혈액 중 혈당량을 육안으로 측정할 수 있음은 물론 자동 분석기기의 기본 시료로 이용될 수 있음을 확인할 수 있었다. We have developed a simple and accurate strip test that measures the blood glucose level semiquantitatively by visual observation, or qualitatively by using UltraScan spectrocolorimeter. The strip has solid phase reagents, including glucose oxidase, peroxidase, chromogen, affixed to a plastic support. The strip test is capable of measuring blood glucose level in the range of 0∼800 mg/dl and generating the results within 2 to 3 minutes. Human blood specimens obtained from normal individuals and the diabetic patients were evaluated by the new blood glucose strip and by the kit supplied by other commercial products. The test results exhibit the correlation coefficient of 0.964. The new test strip is proven simple and accurate, and it offers an alternative to the commercially available glucose tests.

L1210 세포증식에 대한 Glycyrrhizin의 억제작용 기전

殷載淳,徐龍勳,權鎭,柳東和,吳贊鎬,蘇俊魯,全焄,黃甲洙 우석대학교 의약품개발연구소 1996 藥學硏究誌 Vol.1 No.-

The purpose of this research was to investigate the mechanism of inhibitory action of Glycyrrhizin(GZ) on the proliferation of mouse leukemia cell-line, L1210 cells. The cytotoxic activity was tested using a colorimetric tertrazolium assay(MTT assay), the apoptosis was tested using flow cytometry. Nitric oxide(NO) production form mouse peritoneal macrophage was tested using a Griess method and the phagocytosis of human polymorphonuclear cells was tested using a lucigenin chemiluminescence. GZ ingibited the proliferation of L1210, BALB/c 3T3 cells and mouse thymocytes at 50 ug/ml/ GZ did not affect nitric oxide production from mouse peritoneal macrophages in vitro, but ingibited nitric oxide production from lipopolysaccharide and y-interferon treated macrophages. Macrophages of GZ-administered mice accelerated NO production. The proliferation of L1210 cells apoptosis of L1210 cells were induced by co-culture with macrophage of GZ-administered mice. The apoptosis of L1210 cells were induced by co-culture with macrophage of GZ-administered mice. GZ increased the phagocytosis of human polymorphonuclear cells. These results suggest that GZ inhibit the proliferation of L1210 cells not only as a direct cytotoxic agent o tumor cells, but also by the enhancement of NO production and phagocytic activity.

家兎肺 抽出液과 洗滌液의 表面活性度와 In Vitro X線照射가이에 미치는 影響

權得基,朱永恩 慶北大學校 醫科大學 1975 慶北醫大誌 Vol.16 No.1

家兎肺에서 얻은 推出液(extracts)과 洗滌液(washings)의 肺胞表面 活性物質의 活性度와 蛋白含量을 比 較하고, 나아가서 300R, 600R 및 1,000R의 X線을 in Vitro로 照射하여 20℃에서 1,4,8,12 및 24時間에 各各 表面活性度와 蛋白含量을 測定하여 果 다음과 같은 結果를 얻었다. 1. 正常對照群의 肺洗滌液의 最大 및 最小 表面張力, 幅, S^- 및 蛋白含量은 各各 44.1dyne/cm, 8.0dyne/cm, 14.2dyne/cm, 1.38 및 115.8mg/ml이었고 肺推出液의 그것들은 各各 36.8dyne/cm, 13.6dyne/cm, 9.2dyne/cm, 0.91 및 884.7mg/ml이었다. 2. 肺洗滌液이나 肺推出液에서 모두 20℃에서 實驗 24時間까지 肺胞表面活性度나 蛋白含量은 큰 變化를 나타내지 않았다. 3. 300R의 in Vitro X線 照射로서는 各 實驗値가 對照에 比하여 큰 差異를 나타내지 않았으나, 600R 또 는 1,000R의 in Vitro對照로서는 對照群에 比하여 各實驗値에서 若干의 差異를 나타내었으며, 特히 最小 張力에서 그 傾向이 뚜렷하였다. 4. 以上의 結果로서, 肺胞表面活性度測定에는 肺推出液보다 肺洗滌液을 使用함이 더 有利하고 20℃에서 肺洗滌液을 保存하여도 그 活性度나 蛋白含量에 差異가 없음을 알 수 있다. 또한 X線 in Vitro 照射는 肺胞表面活性物質에 큰 變化를 나타내지 않는 것이라고 思料된다. The extraction method of preparing the pulmonary surfactant is crucial in determining and evaluating the surfactant activities in situ. In the present study, two most commonly used methods were compared; namely, the extracts and washings. Also, in an attempt to clarify the effect of X-irradiation on the pulmonary surfactant system, X-ray of 300R, 600R and 1,000R, respectively was irradiated to the lung extracts and lung washings obtained from the rabbit in Vitro, and the pulmonary surfactant activities as well as the protein contents of both extracts and washings were studied at 1, 4, 8, 12 and 24 hours after the irradiation at 20℃. The results obtained are summarized as follows: 1) The maximal and minimal surface tensions, width of the tension-area diagram at the surface area of 40%, stability index and protein content of the lung washings in the non-irradiated normal group was 44.1 dyne/cm, 8.0 dyne/cm, 14.2 dyne/cm, 1.38 and 115.8 mg/ml, respectively. In the lung extracts, the value was 36.8dyne/cm, 13.6dyne/cm, 9.2dyen/cm, 0.911and 884.7mg/ml, respectively. 2) In the normal group, both the lung washings and lung extracts showed no noticeable change in the pulmonary surfactant activities and protein contents upto 24 experimental hours at 20℃. 3) X-irradiation of 300R in Vitro produced no meaningful change in the surfactant activities or protein contents from the control, but 600R and 1,000R of X-irradiation showed a tendency of sligt changes in the surfactant activities from the control with the increased values of the minimal surface tensions. 4) From the above, it may be considered that the lung washings are better suited than the lung extracts for the measurement of the pulmonary surfactant activities. Also, at 20℃, the pulmonary surfactant activities as well as the protein contents do not seem to be altered greatly for at least 24 hours. Finally, X-irradiation in Vitro does not seem to produce any noticeable change in the pulmonary surfactant.

포커스 그룹을 이용만 임상간호사의 임파워먼트 경험에 관한 연구

권성복,염영희,권은경,이윤영 대한간호학회 간호행정학회 2007 간호행정학회지 Vol.13 No.4

Purpose: This study was to explore the empowerment experienced by hospital nurses. Methods: Focus group was used to gather data and open coding method suggested by Strauss and Corbin was used to analyze the data. Thirty-four nurses who have clinical experience of more than three years at three university hospitals were participated in this study. Results: Five categories were identified: Getting chances of opportunities to show personal capabilities and motivations, acknowledgement, support, recognition and delightfulness. The major sources of empowerment were the acknowledgement of patients and family, colleagues, supervisors. doctors, and other personnels in hospital. Conclusion: Focus groups could be an useful tool for empowerment research in nursing. further research will be needed to clarify the relationship among individual, group and organization.