Study on the Elastic Characteristics of Living Cells using Atomic Force Microscope Indentation Technique

Kwon Eun-Young,Kim Young-Tae,Kim Dae-Eun Korean Tribology Society 2006 KSTLE International Journal Vol.7 No.1

In this work, imaging and study of elastic property of the living cell was performed. The motivation of this work was to seek the possibility of exploiting Young's modulus as a disease indicator using Atomic Force Microscope (AFM) and also to gain fundamental understanding of cell mechanics for applications in medical nanorobots of the future. L-929 fibroblast adherent cell was used as the sample. Imaging condition in cell culturing media environment was done in very low speed ($20{\mu}m/ s$) compared to that in the ambient environment. For measuring the Young's modulus of the living cell, AFM indentation method was used. From the force-distance curve obtained from the indentation experiment the Young's modulus could be derived using the Hertz model. The Young's modulus of living L-929 fibroblast cell was $1.29{\pm}0.2$ kPa.

Study on the Elastic Characteristics of Living Cells using Atomic Force Microscope Indentation Technique

Eun-Young Kwon,Young-Tae Kim,Dae-Eun Kim 한국트라이볼로지학회 2006 KSTLE International Journal Vol.7 No.1

In this work, imaging and study of elastic property of the living cell was performed. The motivation of this work was to seek the possibility of exploiting Young’s modulus as a disease indicator using Atomic Force Microscope (AFM) and also to gain fundamental understanding of cell mechanics for applications in medical nanorobots of the future. L-929 fibroblast adherent cell was used as the sample. Imaging condition in cell culturing media environment was done in very low speed (20 μm/s) compared to that in the ambient environment. For measuring the Young’s modulus of the living cell, AFM indentation method was used. From the force-distance curve obtained from the indentation experiment the Young’s modulus could be derived using the Hertz model. The Young’s modulus of living L-929 fibroblast cell was 1.29 ± 0.2 kPa.

AFM indentation 기법을 이용한 세포의 탄성특성에 관한 연구

권은영(Eun-Young Kwon),김영태(Young-Tae Kim),김대은(Dae-Eun Kim) 한국트라이볼로지학회 2006 한국트라이볼로지학회 학술대회 Vol.2006 No.6

In this work, imaging and investigation of elastic property of the living cell was performed. The motivation of this work was to seek the possibility of exploiting Young's modulus as a disease indicator using Atomic Force Microscope (AFM) and also to gain fundamental understanding of cell mechanics for applications in medical nanorobots of the future. L-929 fibroblast adherent cell was used as the sample. Imaging condition in cell culturing media environment was done in very low speed(20 ㎛/s) compare to that in ambient environment. For measuring the Young's modulus of the living cell, AFM indentation method was used. From the force-distance curve obtained from the indentation experiment the Young's modulus could be derived using the Hertz model. The Young's modulus of living L-929 fibroblast cell was 1.29 ± 0.2 kPa.

P087 : Adverse effects of oral cyclosporine in the treatment of various skin diseases

( Hyung Il Kwon ),( Eun Jin Kim ),( Hyun Woo Kim ),( Hyun Chul Park ),( Jeong Eun Kim ),( Joo Yeon Ko ),( Young Suck Ro ) 대한피부과학회 2013 대한피부과학회 학술발표대회집 Vol.65 No.2

Background: Cyclosporine (CS) has been used for various skin diseases including psoriasis and atopic dermatitis. However, dermatologists are reluctant to use CS in clinics because of the adverse effects. Objectives: To investigate the adverse effects of CS for dermatological uses. Methods: A retrospective study of 1,335 patients with CS treatment was performed. Results: 15.6% of patients showed adverse effects. Gastrointestinal (GI) symptoms were the most common adverse effect. Other adverse effects included hypercholesterolemia, hypertrichosis, headache, hypertension, hypertriglyceridemia in order of frequency. Hypercholesterolemia was more often in men, but GI symptoms and hypertrichosis in women. Hypercholesterolemia, hypertriglyceridemia and GI symptoms were more common in adults, but hypertrichosis in young. GI symptoms and headache occurred more often within three months of CS treatment, but hypertension, hypercholesterolemia and hypertriglyceridemia after three months. Hypertrichosis was more often in group with low initial dose. GI symptoms occurred more often with lower CS cumulative dose group and hypertension and hypertriglyceridemia in higher CS cumulative dose group. Conclusion: Comparing to previous studies, the frequency of adverse effects of CS was proven to be low. Therefore, in dermatologic field, we suggest that CS is relatively safe with periodic follow-up.

장기간 항갑상선제 투여 후 임의 중단 뒤 재복용시 발생한 무과립구증 1 예

김승만;김태영;박수영;배영환;서영경 정은주;김태균;권민정;박정현;이순희 인제대학교 2011 仁濟醫學 Vol.32 No.-

Agranulocytosis, one of the major side effects of antithyroid a gents, probably occurs in less than 0.25% of cases treated with antithyroid agents. It usually occurs within the first several weeks or months after the initiation of therapy, when drug dosage is higher. Most reported cases of agranulocytosis have occurred during the first 3 months of therapy. We describe here a rare case that did not experience the side effect for 6 years of antithyroid agent treatment but presented with agranulocytosis in one month after re-exposure. It suggests that the major side effect such as agranulocytosis can occur in patients who had undergone prior uneventful antithyroid drug therapy.

Acetyl CoA carboxylase의 활성 조절에 있어서 Covalent phosphorylation과 Deploymerization간의 상관관계에 관한 연구

권경렬,김은영,배동훈 단국대학교 신소재기술연구소 1995 신소재 Vol.5 No.-

지방산 합성계의 지배 효소인 Acetyl CoA carboxylase의 활성조절에는 hormone에 의한 covalent modification과 세포내 대사물질에 의한 조절이 관여하는 것으로 알려져 왔다. Allosteric regulation과 covalent modification 두 경우 모두 중합체와 이량체로서 상호전환 되며 효소활성이 변환된다. BSA를 효소의 인산화과정에 함께 사용하였을 경우는 특이하게도 효소의 인산화는 일어나나 이량체로서의 분해는 일어나지 않는다. 이러한 사실로부터 효소 활성조절은 직접적으로 polymerization-depolymerization 변환에 의해 나타내며 인산화-탈인산화는 효소의 4차 구조를 변환시키기 위한 도구로서 사용되며 인산화-탈인산화에 의하여 활성이 직접적으로 변화하지는 않는다. Acetyl CoA carboxylase, the rate-limiting enzyme in long chain fatty acid synthesis, exhibits complex regulatory properties. Hormonal regulation of the carboxylase activity and thus fatty acid sythesis is mediated by the covalent modification of the enzyme, and cellular metabolites regulate the carboxylase activity through polymerization-depolymerization of the enzyme. In this paper, we show the evidences that the covalent modification of Acetyl CoA carboxylase is a means of changing the quaternary structure of the carboxylase and not the activity per se.

장기기증 희망자의 의사결정 경험

권영미,윤은자 성인간호학회 2003 성인간호학회지 Vol.15 No.2

The aim of this study was to identify the process of experience toward decision making to donate organs. Method: Eleven subjects who registered at the Association for Organ Sharing in Korea were recruited. Using in-depth interviews and tape-recordings. Data was collected from Jun. 2001 to Feb. 2002 and the contents of these were analyzed by Glaser's grounded theory analysis method. Result: Basic Social- Psychological Problem(BSPP) of subjects were 'attachment to body' and the core theme, that is Basic Socialization Progress(BSP) were discovered to 'find out what is the meaning of life' Also it consisted of four steps: 1st 'hesitate' 2nd 'look into self'. 3rd 'realize the tie up to self' and last 're-find the meaning of life'. Conclusion: These findings indicate the Korean's Basic Socialization Progress of decision making regarding organ donation, so possible development of promoting decision making is necessary strategies for people who is having his/her potential of organ donation in mind.

벼 상온 저장 시기별 쌀 품질 변화

권석주,송영은,권영립,이덕렬,조승현,최동철 원광대학교 2005 論文集 Vol.35 No.-

Increasing concern about a rice quality, it is considered postharvest technology as well as cultivation management as an important factor. Therefore it is necessary to find optimal drying, storage method that did not affected a rice quality. In order to find the optimum storage period of rice, the rice quality was investigated during storage period. The temperature and humidity of storage bin were greatly changed but those of rice were a little do. The germination rate was slowly decreased as the storage period is long. The content of moisture was affected by a room temperature, it was increased at stored rice in rainy season(10-Jul~20-Jul). As storage period is long, the content of amylose, fatty acidity were a just Little changed but did not showed a constant tendency to it. The content of protein was increased and mechanic palatability value was decreased during storage period. The chromaticity of milled rice showed a range of lightness(L); 75.15~71.75, redness(a); 0.21~0.91, yellowness(b); 16.78~19.28 during storage period.

물류서비스 품질이 고객만족에 미치는 영향

권은영,문상영 동의대학교 경제경영전략연구소 2010 經濟經營硏究 Vol.5 No.1

The purpose of this study is to identify effects of logistics service quality on customer satisfaction in online sales company. Since korean customer lay weight on an personal relationship rather than various other factors for service quality, different approach for customer service in korean online sales company compared to online sales company in advance countries should be considered. To do so, previous studies are broadly reviewed

Alkylhalide가 Mouse의 LDH isozyme 분포에 미치는 영향 : Cellulose Acetate Electrophoresis Method

권미경,김영옥,박은주,이동화,이명미,이문희,이양자 曉星女子大學校 藥學大學 學生會 1988 曉星藥誌 Vol.4 No.-

The effect of Carbontetrachloride administration on Lactatedehydrogenase isozyme patterns were analyzed by cellulose acetate electrophoresis for the brain, heart, kidney, lung, liver and mouse muscle. Each other tissue was found to have a characteristic distribution of these isozymes. At normal condition, aerobic tissue such as brain, heart, kidney, and lung were found to have all five isozymes from LDH_1 to LDH_5, while anaerobic tissues such as liver and muscle were found to have superiorly LDH_5 The toxicity of Carbontetrachloride administration was most prominence in the liver, and lung toxicity was occured also.