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RISS 인기검색어
- 검색키워드
- 저자 : Zhibin Ji (검색결과 4 건)
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miR-143 inhibits proliferation and induces apoptosis of mammary epithelial cells in dairy goat
Zhibin Ji,Guizhi Wang,Lei Hou,Zhaohua Liu,Jianmin Wang,Tianle Chao 한국통합생물학회 2016 Animal cells and systems Vol.20 No.2
MicroRNAs are a class of post-transcriptional regulators of gene expression in multicellular organisms, which play important roles in cell fate, organ morphogenesis and carcinogenesis. In the present study, we demonstrated the critical roles of miR-143 on mammary epithelial cells of dairy goat. The test results of cell count, methylthiazolyldiphenyl-tetrazolium bromide, Hoechst33342/PI (propidium iodide) and flow cytometry showed miR-143-induced G0/G1 phase arrest, inhibited proliferation and promoted apoptosis of mammary epithelial cells; the qRT-PCR test of marker genes related to cell proliferation and apoptosis, BAX and BCL-2, supported the same conclusions. Our study presents evidence that miR-143 is an important post-transcription regulator involving in mammary cells survival, and it may have a value function in mammary gland development, lactation or involution.
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Identification and Function Prediction of Novel MicroRNAs in Laoshan Dairy Goats
Ji, Zhibin,Wang, Guizhi,Zhang, Chunlan,Xie, Zhijing,Liu, Zhaohua,Wang, Jianmin Asian Australasian Association of Animal Productio 2013 Animal Bioscience Vol.26 No.3
MicroRNAs are a class of endogenous small RNAs that play important roles in post-transcriptional gene regulation by directing degradation of mRNAs or facilitating repression of target gene translation. In this study, three small RNA cDNA libraries from the mammary gland tissues of Laoshan dairy goats (Capra hircus) were constructed and sequenced, individually. Through Solexa high-throughput sequencing and bioinformatics analysis, we obtained 50 presumptive novel miRNAs candidates, and 55,448 putative target genes were predicted. GO annotations and KEGG pathway analyses showed the majority of target genes were involved in various biological processes and metabolic pathways. Our results discovered more information about the regulation network between miRNAs and mRNAs and paved a foundation for the molecular genetics of mammary gland development in goats.
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Wang, Jianli,Song, Yilin,Zhang, Cheng,Ye, Zhibin,Liu, Hui,Lee, Myong-Hoon,Wang, Dehai,Ji, Jianbing WILEY-VCH Verlag 2008 Macromolecular Chemistry and Physics Vol.209 No.14
<P>A novel alternating copolymer of sulfonated poly(ether ether ketone-benzimidazole)s (SPEEK-BI) was synthesized by polycondensation of aromatic bisphenol containing benzimidazole and a mixture of sulfonated and non-sulfonated difluorobenzophenones. By varying the amount of two difluorobenzophenones, the ratio of sulfonic acid unit to imidazole base was controlled straightforwardly. The polymer structure was characterized by <SUP>1</SUP>H NMR and IR spectroscopy. Two different methods of ion exchange capacity measurement supported a strong inter- and intra-molecular interaction between acid and base units, which resulted in an improved mechanical strength as well as avoiding phase separation. Tough membranes with good mechanical stability in hot water (80 °C) and alcohol were obtained by solution casting, which is potential for applications in polymer electrolyte membrane fuel cells and membranes for separation of liquids or gases.</P><P> <img src='wiley_img/10221352-2008-209-14-MACP200700610-gra001.gif' alt='wiley_img/10221352-2008-209-14-MACP200700610-gra001'> </P>
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Hak Cho Kim,Jong-Wook Kwon,Zhibin Zhou,Ji Hyun Heo 한국무역학회 2019 Journal of Korea trade Vol.23 No.7
Purpose - This study empirically investigated whether national distances (Cultural, Administrative, Geographic and Economic distance) have different effects on industries in different ways. Design/methodology - The empirical model utilized industry level export trade data to examine the differentiated effects of each national distance. By using direct measurement and covering 75 countries, this paper analyzes the effect of distances in different industries. More specifically, this study classified the industries into a more-sensitive/less-sensitive scale. Findings - The empirical results showed the complicated impacts of national distances on trade. Trade in industries that are more sensitive to cultural and administrative distances was not decreased as greatly as trade in industries that are less sensitive to cultural and administrative distances. Also, industries that are more sensitive to geographic distance showed a stronger negative impact on trade than less sensitive industries. Lastly, economic distance decreased trade for industries that are more sensitive to economic distance, while economic distance did not significantly increase trade for industries that are less sensitive to economic distance. Originality/value - As the first study to empirically find out whether national distance has different effects on industries, this study updated the measurements of national distance.
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