RISS 학술연구정보서비스

검색
다국어 입력

http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.

변환된 중국어를 복사하여 사용하시면 됩니다.

예시)
  • 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
  • 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
닫기
    인기검색어 순위 펼치기

    RISS 인기검색어

      검색결과 좁혀 보기

      • 좁혀본 항목

      • 좁혀본 항목 보기순서

        • 원문유무
        • 음성지원유무
          • 원문제공처
          • 등재정보
          • 학술지명
          • 주제분류
          • 발행연도
          • 작성언어
          • 저자

        오늘 본 자료

        • 오늘 본 자료가 없습니다.
        더보기
        • 무료
        • 기관 내 무료
        • 유료
        • 배추 시스테인 단백질 분해 효소 억제 유전자 BCPI-1의 발현과 종자 발아 및 유묘 생장과의 연관성

          홍준기,이은영,김정률,양경애,최영주,정우식,김호일,윤대진,이상열,조무제,임체오 Plant molecular biology and biotechnology research 2003 Plant molecular biology and biotechnology research Vol.2003 No.-

          Phytocystatins are protein inhibitors of cysteine proteinases of the papain family that have been identified in both monocot and dicot plants. A cDNA encoding a phytocystatin, BCPI-1 (Brassica Cysteine Proteinase Inhibitor-1) has been isolated from Chinese cabbage (Brassica campestris L. ssp. pekinensis) flower buds. Here, we tested whether BCPI-1 transcription is regulated by hormones, and could be involved in regulating cysteine proteinases during and after germination. BCPI-1 was sensitive to exogenous GA_(3) and ABA, which are important factors controlling seed germination, suggesting that the expression of BCPI-1 is hormonally regulated. We introduced a recombinant plasmid containing the full-length BCPI-1 cDNA under the control of cauliflower mosaic virus 35S promoter into rice embryogenic calli using the particle delivery method, and regenerated a number of transgenic rice plants. Constitutively over-expressed BCPI-1 caused changes in overall plant growth and development, including reduced germination and seedling growth. These data support the role of the BCPI-1 in the regulation of endogenous proteinases during both seed germination and subsequent seedling development. Phylocystatins은 papain계열의 cysteine 단백질 분해 효소 활성을 특이적으로 억제하는 억제자로 다양한 식물 종으로부터 분리되었다. 본고에서는, 배추 화아 cDNA library로부터 분리된 phytocystatin인 BCPI-1(Brassica Cysteine Proteinase Inhibitor-1)의 생체 내 기능에 대해 연구하였다. 먼저, BCPI-1 전사체는 발아 조절에 중요한 영향을 미치는 GA₃와 ABA에 의해 예민하게 증가, 혹은 감소되는 반응을 보임으로써, BCPI-1이 식물 호르몬의 영향을 받으며, 특히 발아나 유묘의 생장 조절에 관련이 있음을 알 수 있었다. Particle bombardment 방법을 통하여 BCPI-1을 벼의 배 형성 세포 내에 도입, 재분화 시켜 형질 전환 벼를 생성하였다. CaMV 35S promoter의 조절에 의해 지속적인 BCPI-1 발현을 보이는 형질 전환 벼의 경우, 발아와 유묘 생장이 현저히 지연되었다. 위의 결과들을 바탕으로, BCPI-1이 식물체 내에서 생성되는 cysteine 계열의 단밸질 분해 효소 활성을 조절하여 종자의 발아와 유묘의 생장에 영향을 미치는 것으로 추측한다.

        • SCIESCOPUSKCI등재

          Optimization of Medium Components for Plasmid Production by Recombinant E. coli $DH5{\alpha}$ $pUK21CMV{\beta}1.2$

          Zheng, Shuzhen,Friehs, Karl,He, Ning,Deng, Xu,Li, Qingbiao,He, Zheng,Xu, Chenyun,Lu, Yinghua Korean Society for Biotechnology and Bioengineerin 2007 Biotechnology and Bioprocess Engineering Vol.12 No.3

          In this study we investigated the effects of carbon and nitrogen sources, and the carbon to nitrogen ratio (C:N) of growth medium on the production of plasmid DNA by $pUK21CMV{\beta}1.2$ harbouring recombinant E. coli. The results indicated that sucrose and casein peptone were the most suitable carbon and nitrogen sources, respectively, for plasmid production, and the plasmid concentration was significantly influenced by the C:N ratio. A Box-Behnken design and surface response methodology were employed to formulate the optimal medium. In the optimized medium, a plasmid concentration of 51.80mg/L and a plasmid selectivity of 23.49mg/g were achieved after 24h of incubation. These values were approximately 3.5 and 1.7 times higher, respectively, than those obtained on the original LB medium.

        • SCIESCOPUSKCI등재

          Purification and Characterization of a Thermostable Xylanase from Paenibacillus sp. NF1 and its Application in Xylooligosaccharides Production

          Zheng, Hong-Chen,Sun, Ming-Zhe,Meng, Ling-Cai,Pei, Hai-Sheng,Zhang, Xiu-Qing,Yan, Zheng,Zeng, Wen-Hui,Zhang, Jing-Sheng,Hu, Jin-Rong,Lu, Fu-Ping,Sun, Jun-She The Korean Society for Microbiology and Biotechnol 2014 Journal of microbiology and biotechnology Vol.24 No.4

          High levels of extracellular xylanase activity (211.79 IU/mg) produced by Paenibacillus sp. NF1 were detected when it was submerged-cultured. After three consecutive purification steps using Octyl-Sepharose, Sephadex G75, and Q-Sepharose columns, a thermostable xylanase (XynNF) was purified to homogeneity and showed a molecular mass of 37 kDa according to SDS-PAGE. The specific activity of the purified XynNF was up to 3,081.05 IU/mg with a 14.55-fold purification. The activity of XynNF was stimulated by $Ca^{2+}$, $Ba^{2+}$, DTT, and ${\beta}$-mercaptoethanol, but was inhibited by $Fe^{2+}$, $Zn^{2+}$, $Fe^{2+}$, $Cu^{2+}$, SDS, and EDTA. The purified XynNF displayed a greater affinity for oat spelt xylan with the maximal enzymatic activity at $60^{\circ}C$ and pH 6.0. XynNF, which was shown to be cellulose-free, with high stability at high temperature ($70^{\circ}C-80^{\circ}C$) and low pH range (pH 4.0-7.0), is potentially valuable for various industrial applications. The enzyme hydrolyzed oat spelt xylan to yield mainly xylooligosaccharides (95.8%) of 2-4 degree of polymerization (DP2-4). Moreover, the majority of the xylooligosacharides (DP2-4) products was xylobiose (61.5%). The thermostable xylanase (XynNF) thus seems potentially usefull in the production of xylooligosaccharides.

        • Over-expression of Chinese cabbage calreticulin 1, BrCRT1, enhances shoot and root regeneration, but retards plant growth in transgenic tobacco

          Zheng-Lu Jin,Hong, Joon-Ki,Yang, Kyung-Ae,Koo, Ja-Choon,Choi, Young-Ju,Chung, Woo-Sik,Yun, Dae-Jin,Lee, Sang-Yeol,Cho, Moo-Je,Lim, Chae-Oh Plant molecular biology and biotechnology research 2005 Plant molecular biology and biotechnology research Vol.2005 No.

          Calreticulin (CRT) is a ubiquitously expressed, high capacity Ca^(2+)-binding protein that is involved in intracellular Ca^(2+) homeostasis and molecular chaperoning in the endoplasmic reticulum (ER). A cDNA encoding a calreticulin, BrCRT1 (Brassica rapa Calreticulin 1), has been isolated from Chinese cabbage (B. rapa subsp. pekinensis) flower bud. Constitutive over-expression of the BrCRT1 gene promotes robust shoot production and root formation at sub-optimal concentrations of BA/NAA, which are important factors controlling plant regeneration in tissue culture. In contrast, the suppressed BrCRT1 line exhibited a slight reduction of shoot and root regeneration. In spite of enhanced regeneration in tissue culture, the seedling and plant growth rate was inhibited in soil. The steady state level of BrCRT1 transcripts was sensitive to exogenous auxins and cytokinins, and rapidly accumulated within 30 min, and this induction required de novo protein synthesis. Together with the results of transgenic tobacco plants and mRNA analysis in Chinese cabbage, our data suggest that BrCRT1 genes may up-regulate the competency of vegetative tissue to respond to hormonal signals involved in shoot and root regeneration processes.

        • SCIESCOPUSKCI등재후보

          A composite crack model for concrete based on meshless method

          Lu, Xin-Zheng,Jiang, Jian-Jing,Ye, Lie-Ping Techno-Press 2006 Structural Engineering and Mechanics, An Int'l Jou Vol.23 No.3

          A crack model for the fracture in concrete based on meshless method is proposed in this paper. The cracks in concrete are classified into micro-cracks or macro-cracks respectively according to their widths, and different numerical approaches are adopted for them. The micro-cracks are represented with smeared crack approach whilst the macro-cracks are represented with discrete cracks that are made up with additional nodes and boundaries. The widely used meshless method, Element-free Galerkin method, is adopted instead of finite element method to model the concrete, so that the discrete crack approach is easier to be implemented with the convenience of arranging node distribution in the meshless method. Rotating-Crack-Model is proved to be preferred over Fixed-Crack-Model for the smeared cracks of this composite crack model due to its better performance on mesh bias. Numerical examples show that this composite crack model can take advantage of the positive characteristics in the smeared and discrete approaches, and overcome some of their disadvantages.

        • KCI등재

          Privatization of State-Controlled Shareholding Companies and the Rise of Corporate Elites in China

          Lu Zheng,Byung Soo Kim 지역사회학회 2017 지역사회학 Vol.18 No.2

          '스콜라' 이용 시 소속기관이 구독 중이 아닌 경우, 오후 4시부터 익일 오전 7시까지 원문보기가 가능합니다.

          As the State still controls a large share of China’s economy, the process of privatization of state-owned enterprises and the rise of corporate elites who control them should be traced and examined in a systematic and thorough way. As one of the first efforts to do so, this research collected and analyzed first-hand data on private-takeovers of 341 previously State-controlled listed firms on China’s stock exchanges during the period of 1996-2008. Preliminary results show privatizations have most likely occurred amongst relatively small-sized firms, more prevalently in industries under less state oversight, and more in economically developed provinces. Further analysis reveals that the majority of the new private controlling shareholders are outsiders rather than insiders to the acquired firms, and as a whole they are highly educated, and politically and economically well positioned. This research suggests that a group of capable private entrepreneurs are assuming roles as new corporate elites by taking advantage of emerging merger and acquisition opportunities in China’s stock market. It is the different route of privatization characterized by the above findings that China has embarked upon and is contributing to its relative success in economic reforms compared with its former communist counterparts.

        • KCI등재

          Correlation of Adventitial Vasa Vasorum with Intracranial Atherosclerosis: A Postmortem Study

          Lu Zheng,Wen Jie Yang,Chun Bo Niu,Hai Lu Zhao,Ka Sing Wong,Thomas Wai Hong Leung,Xiang Yan Chen 대한뇌졸중학회 2018 Journal of stroke Vol.20 No.3

          Background and Purpose Vasa vasorum (VV) have been believed to be rare or non-existent in small-caliber intracranial arteries. In a series of human cerebral artery specimens, we identified and examined the distribution of VV in association with co-existing intracranial atherosclerosis. Methods We obtained cerebral artery specimens from 32 consecutive autopsies of subjects aged 45 years or above. We scrutinized middle cerebral artery (MCA), vertebral artery (VA), and basilar artery (BA) for the presence of adventitial VV. We described the distribution of VV, and the characteristics of co-existing atherosclerotic lesions. Results Among 157 intracranial arteries, adventitial VV were present in 74 of the 157 specimens (47%), involving MCA (n=13, 18%), BA (n=14, 19%), and VA (n=47, 64%). Although qualitatively these 74 adventitial VV distributed similarly in arteries with or without atherosclerotic lesions (disease-free arteries n=4/8; arteries of pre-atherosclerosis n=17/42; and arteries of progressive atherosclerosis n=53/107), the presence of adventitial VV in intracranial VA was associated with a heavier plaque load (1.72±1.66 mm2 vs. 0.40±0.32 mm2, P<0.001), severer luminal stenosis (25%±21% vs. 12%±9%, P=0.002), higher rate of concentric lesions (79% vs. 36%, P=0.002), and denser intraplaque calcification (44% vs. 0%, P=0.003). Histologically, intracranial VA with VV had a larger diameter (3.40±0.79 mm vs. 2.34±0.58 mm, P<0.001), thicker arterial wall (0.31±0.13 mm vs. 0.23±0.06 mm, P=0.002), and a larger intima-media (0.19±0.09 mm vs. 0.13± 0.04 mm, P=0.003) than VA without VV. Conclusions Our study demonstrated the distribution of adventitial VV within brain vasculature and association between vertebral VV and progressive atherosclerotic lesions with a heavier plaque load and denser intraplaque calcification.

        • KCI등재
        • SCIESCOPUS

          Numerical validation of Multiplex Acceleration Model for earthquake induced landslides

          Zheng, Lu,Chen, Guangqi,Zen, Kouki,Kasama, Kiyonobu Techno-Press 2012 Geomechanics & engineering Vol.4 No.1

          Due to strong ground motion of earthquake, the material in the landslide can travel a significant distance from the source. A new landslide model called Multiplex Acceleration Model (MAM) has been proposed to interpret the mechanism of long run-out movement of this type of landslide, considering earthquake behaviors on slope and landslide materials. In previous study, this model was verified by a shaking table test. However, there is a scale limitation of shaking table test to investigate MAM in detail. Thus, numerical simulation was carried out in this study to validate MAM under full scale. A huge rock ejected and A truck threw upwards by seismic force during Wenchuan Earthquake (Ms. 8.0) was discussed based on the simulation results. The results indicate that collisions in P-phase of earthquake and trampoline effect are important behaviors to interpret the mechanism of long run-out and high velocity. The results show that MAM is acceptable and applicable.

        • SCIESCOPUSKCI등재

          Integrating Granger Causality and Vector Auto-Regression for Traffic Prediction of Large-Scale WLANs

          Lu, Zheng,Zhou, Chen,Wu, Jing,Jiang, Hao,Cui, Songyue Korean Society for Internet Information 2016 KSII Transactions on Internet and Information Syst Vol.10 No.1

          Flexible large-scale WLANs are now widely deployed in crowded and highly mobile places such as campus, airport, shopping mall and company etc. But network management is hard for large-scale WLANs due to highly uneven interference and throughput among links. So the traffic is difficult to predict accurately. In the paper, through analysis of traffic in two real large-scale WLANs, Granger Causality is found in both scenarios. In combination with information entropy, it shows that the traffic prediction of target AP considering Granger Causality can be more predictable than that utilizing target AP alone, or that of considering irrelevant APs. So We develops new method -Granger Causality and Vector Auto-Regression (GCVAR), which takes APs series sharing Granger Causality based on Vector Auto-regression (VAR) into account, to predict the traffic flow in two real scenarios, thus redundant and noise introduced by multivariate time series could be removed. Experiments show that GCVAR is much more effective compared to that of traditional univariate time series (e.g. ARIMA, WARIMA). In particular, GCVAR consumes two orders of magnitude less than that caused by ARIMA/WARIMA.

        맨 위로 스크롤 이동