비병원성 야생효모, Apiotrichum sacrabaeorum WP49-1로부터 β-Glucosidase 생산

신유리, 장지은, 이종수 배재대학교 자연과학연구소 2016 自然科學論文集 Vol.32 No.1

콩에 함유되어 있는 식물성 여성 호르몬인 estrogen의 흡수를 증가시키기 위해 높은 활성의 β-glucosidase를 생산하는 비병원성 야생 효모의 선별과 β-glucosidase의 생산 조건을 조사하였다. 38종의 비병원성 야생효모 중 Apiotrichum scarabaeorum WP49-1의 무세포 추출물이 6.5 unit의 가장 높은 β-glucosidase 활성을 보였고 Zygosaccharomyces rouxii O-5-7도 5.9 Unit의 높은 활성을 보였다. 최종적으로 β-glucosidase 생성 효모 균주로 Apiotrichum scarabaeorum WP49-1 균주를 선별하였다. β-Glucosidase는 Apiotrichum scarabaeorum WP49-1을 yeast extract-peptone-dextrose 배지에 접종하여 30℃에서 72시간 배양했을 때 최대로 생성되었다. 선발 균주는 구형으로 출아에 의한 영양증식을 하였고 포자를 형성하지 않았다. 또한 비타민이 함유되어 있지 않은 배지에서도 생육하였고 5% 에탄올에 대하여 내성을 보였다

완전탈구된 치아의 지연 재석

한유리,최형준,이제호,최병재 大韓小兒齒科學會 2002 大韓小兒齒科學會誌 Vol.29 No.4

완전탈구된 치아의 치료법은 재식술을 들 수 있는데, 이는 치조와에서 이탈된 치아를 가능한 빠른 시간 내에 재식립하는 술식이다. 일반적으로 재식술의 성공여부는 치수와 치주인대 손상의 정도, 치아상실 시부터 치조와 내로 재식될 때까지의 경과시간, 탈구된 치아의 보관 상태, 치근의 발육 정도 등에 좌우된다. 그 중 치아상실 시부터 재식까지의 경과시간이 특히 중요한데, 일반적으로 30분 이내에 재식을 시행한 경우에는 90%이상의 성공률을 보인 반면 30분에서 90분 사이에서는 43%, 90분 이상 경과 시에는 7%의 성공률을 보인다고 알려져 있다. 본 증례는 완전탈구 후 오랜 시간이 지연되어 재식을 시행하였으나 치근유착을 통해 비교적 양호한 임상적 결과를 얻었다. 이러한 치료로 결국은 치아의 손실이 일어날지라도, 이것은 환자 및 보호자의 사고에 따른 충격을 감소시켜주고, 자체로 공간 유지장치로서의 기능을 할 수 있으며, 발치에 따른 치조골의 흡수를 늦추는 한편, 적극적인 보철치료를 위한 시간을 벌어줄 수 있을 것으로 사료된다. Replantation may be treatment choice for a completely avulsed tooth caused by traumatic injury. The outcome of replantation depends on the following factors: minimal damage to pulp and periodontal membrane the length of time the avulsed tooth was out of the mouth, how the tooth was stored the level of root formation, etc. The time from the act of avulsion of the tooth to the actual replantation is especially important. Generally, when replanted within 30 minutes, more than 90% of cases succeed but when the time is between 30 to 90minutes 43% and greater than 90 minutes 7%. This is a case of a replanted tooth with relatively good prognois by ankylosis though there was a great time lapse since the tooth was avulsed. Though such treatment lead to loss of the tooth in case of children or adolescents this treatment is meaningful because it may earn time until any definitive therapy functionally stimulate the alveolar bone to retain its hight for a better prognosis for future treatment and act as space maintainer

랫드에서 법제유황과 비법제유황의 독성비교

김근수, 정유리, 이남진, 홍성희, 유종훈, 정재황, 강종구 충북대학교 동물의학연구소 2006 Journal of Biomedical and Translational Research Vol.7 No.2

본문참고

동아시아 국가의 가족정책 비교연구 : 한국·일본·중국·싱가포르·홍콩을 중심으로

양옥경,이유리 梨花女子大學校 社會科學大學 社會科學硏究所 2005 사회과학연구논총 Vol.14 No.-

동아시아 국가들에서 가족은 급격한 사회변화를 겪어왔다. 정치·경제·문화 등 다양한 사회구조와 상호작용함과 동시에 사회발전과 정책변화에도 막대한 영향을 미치는 핵심적인 요인으로서 작용해왔으며 복지생산의 기본적 단위로서의 위상도 지니고 있다. 그럼에도 동아시아 가족관련 연구에 대한 체계적인 비교연구는 부재했었고, 가족정책 전반에 대한 기초적인 사실조차 제대로 소개되지 않은 것이 현실이었다. 이 논문은 향후 본격적인 동아시아 가족정책 비교연구를 위한 출발점으로서, 한국·일본·중국·싱가포르·홍콩 의 가족구조 및 가족가치관에 대한 최근 동향과 변화추이를 개괄적으로 조망하고 그러한 변화에 대응하는 가족정책을 체계적으로 비교분석하고자 하였다. 여기에는 한국, 일본, 중국, 싱가포르, 홍콩정부가 발간한 공식통계자료와 각국 가족관련 연구 자료가 활용되었다. 연구의 결과, 첫째, 급속한 산업화와 근대화의 영향에 대한 가족 구조, 여성의 지위 등의 변화추이와 방향은 동아시아 국가들에서 매우 유사한 형태로 나타나고 있었다. 둘째, 동아시아 국가 모두 가족관련 가치관은 개방적으로 변화하고 있지만, 보수적·가족주의적 성향도 존재하고 있어 아직까지 서구적인 핵가족의 모습으로 변화하였다고는 볼 수 없음을 확인할 수 있었다. 셋째, 동아시아 국가에는 직접적이고 명백한 가족정책이 존재하지는 않지만, 사회보장 체계 안에서 소득지원, 자녀양육지원, 노인부양지원의 형태로 가족을 둘러싼 변화에 대응하고 가족기능을 유지하기 위한 정책들을 펼쳐나가고 있었다. In many East Asian countries, family experiences a rapid social change and plays a fundamental role of welfare production as a key factor that interacts with politics, economy, and culture while it affects social developments and political changes. Nonetheless, an absence of the systematic comparative study and an insufficiency of introducing basic actualities of the family policy have been made in our reality. This study will provide a starting point for the study of East Asian family policy. It will take a general view of the recent tendency and the transition of Korean, Japanese, Chinese, Singaporean and Hongkongite family Structure and Family Value. The result of this study are sum-up as follows. First, the changing pattern and the direction of the family structure, woman's status on the rapid industralization and modernization is appearing as a similar pattern among the East Asian countries. Second, in the East Asian countries, changing of the family value becomes openly; however, the continuities of some traditional attitudes and values on the family have been found in all the East Asia, because conservative familism still exist and East Asian family is hard to identified as Westernized nuclear family. Third, direct and obvious family policy does not exist among the East Asian countries, but family policy to confront family changes and maintain family functions as a form of income supplementation, child care assistance, elderly care assistance has been practiced in the socal welfare system.

주부의 인터넷 쇼핑의 유용성 인지가 활용도에 미치는 영향

박미석,이유리 숙명여자대학교 2000 生活科學硏究誌 Vol.15 No.-

This study analyzes the trend of the awareness of usefulness and usage frequency of Internet shopping and closely examines the influential factors of the trend. In addition, in this study the usage rate of Internet shopping are divided into that of search and purchase, so that the relationships of variables are easily derived through a process matrix. After all, the purpose of this methodology was to provide informatized housewives and household with fundamental data by finding a way to increase the awareness of usefulness and usage frequency of Internet shopping. The major findings are follows: (1) Those who are college-educated, married and employed, positive about information household, Internet-educated, highly capable of the Internet, and comfortable with an issue of an effluence of personal data showed high awareness of usefulness of Internet shopping. (2) Those who are young, college graduate or above, and employed showed the high usage rate of search. Those who are age 20 to 30, college graduate or above, employed, and at the early stage of family life style showed the high usage rate of purchase. In addition, those who are positive about information household, high in the tendency of innovation and the awareness of time constraints, exposed to the Internet education, high in the Internet capability, and low in a burden of the Internet bills and the effulence of personal data showed the high usage rate of search and purchase. (3) An awareness of time constraints and a concern for the effulence of personal data directly influence the usage rate of purchase and indirectly influence the usage awareness of internet shopping. And an attitude toward information household' and Internet capability directly influence the usage rate of search and purchase and indirectly influence the usage awareness of internet shopping. Therefore, according to the result of process matrix, an awareness of usefulness of Internet shopping, an attitude of information household, Internet capability are the variables that influence the usage rate of Internet shopping.

인체 췌장암세포주(Capan-1)의 증식에 미치는 amiloride의 억제효과

임대관,김신,김유리,노지훈,이지현,김지연,박무인,정근옥,박건영,구자영 고신대학교 의학부 2004 高神大學校 醫學部 論文集 Vol.19 No.1

Background/Aims Cytoplasmic alkalinization induced by activation of the Na+/H+anti porter which is stimulated upon the addition of growth-promoting agents, such as insulin, epidermal growth factor, phorbol ester, plays an essential role in the initiation on cell proliferation. In the present study the effects of amiloride, a specific and reversible inhibitor of Na+/H+antiporter, on the growth of human pancreatic carcinoma cell line, Capan-1 cells was examined and the effects of 5-fluorouracil (5-FU) were also studied. Cell cycle analysis was done to examine the mechanisms for the inhibitory effects of amiloride. Materials/Methods The growth of Capan-1 cells were examined by counting cell number on two and four days treatment with 1 μM, 5 μM, 10 μM, 20 μM, 40 μM, 80 μM, 160 μM amiloride, and 0.1 ㎍/㎖, 0.3 ㎍/㎖ 5-FU, after plating Capan-1 cells into 35-mm2 plastic dishes at d density of 10x104 cells/dish. The reversibility of the effects of amiloride was examined on two day to eight days treatment with 20 μM amiloride after seeding 2×104 cells/dish. Cell cycle analysis was done on the sells after four days treatment with 20 μM amiloride. Results Amiloride significantly inhibited the growth of Capan-1 cells in a dose-dependent fashion (p<0.05). The inhibitary effect of amiloride on the growth of Capan -1 cells was firstly shown at the concentration of 5 μM, which is not so higher than the concentration of 0.1-0.2 μM attainable by administration of usual dose of amiloride (5-10㎎). Forty-eight percent inhibition of growth was found at an amiloride concentration of 20μM after 4 days treatment, and ninety-three percent inhibition of growth was found at an amiloride concentration of 160μM after 4 days treatment. The inhibitory effect of amiloride on growth of Capan-1 cells was reversible since removal of amiloride by a media change after 48 hours treatment lead to significantly more growth than amiloride treated group (p<0.05). The reversibility of growth inhibition suggests that amiloride in not a non-specific cytotoxin for Capan-1 cells. Amiloride combined with 5-FU significantly inhibited the growth of Capan-1 cells in a dose-dependent fashion compared to an amiloride or a 5-FU alone (p<0.05). After four days treatment with 20 μM amiloride, the faction of cells in G0-G1 phase, S phase and G2-M phase was 47.3%, 35.8%, 16.9% respectively in the amiloride group (20 μM), and 44.3%, 37.1%, 18.6% in the control group. showing no significant differences between the two groups. Conclusions Amiloride significantly inhibited the growth of Capan-1 cells in a dose-dependent fashion, which was reversible. The reversibility of growth inhibition suggests that amiloride is not a non-specific cytotoxin for Capan-1 cells. The concentration of 5 μM, which is not so higher than the concentration of 0.1-0.2 μM attainable by administration of usual dose of amiloride (5-10㎎), which suggests amiloride or its analogues may be used alone or in conjunction with 5-FU for the treatment of pancreatic carcinoma. Further study is needed to clarify the effects of more potent analogues of amiloride on the growth of human pancreatic carcinoma cells.

P563 : Eosinophilic cellulitis Like injection site reaction to the administration of vancomycin

( Yu Ri Woo ),( Jae Hwa Kim ),( Dong Ha Kim ),( Hae Min Lee ),( Joong Sun Lee ),( Dae Won Koo ),( Kyung Eun Jung ) 대한피부과학회 2014 대한피부과학회 학술발표대회집 Vol.66 No.1

Blue Jean Consumers' Brand Switching Behavior in Out-of-stock Situation

Lee, Yu Ri,Doris H. Kincade 한국의류학회 1999 한국의류학회지 Vol.23 No.8

본 연구는 청바지 제품 소비자가 쇼핑시 제품이 품절된 상황에 접했을 때 보여주는 상표 전환행동에 관한 것이다. 의류 매장 관리자의 적시 적량의 제품 입고 관리 능력이 부족할 때 발생하게 될 손실을 유추해 보고 어떤 유형의 소비자가 상표 전환을 쉽게 일으키는지를 규명하는 것은 더욱 효과적인 매장 운영, 소비자 만족과 고객 관리를 위해 의미있는 일일 것이다. 본 연구는 다음과 같은 목적을 가진다. 1) 제품 품절시 소비자들은 어떤 상표전환 행동을 보이는지 밝힌다. 2) 제품 품절시 대체 상표제품을 구매할 경우 대체 상표 제품의 가격의 영향력이 상표전환 행동과 어떤 관련을 보이는지 밝힌다. 3) 소비자의 쇼핑지향성, 상표 충성, 애고 행동, 인구통제적 특성(성별)이 상표전환 행동에 미치는 영향력을 밝힌다. 이러한 목적을 위해 본 연구는 1997년 3월, 버지니아주 블랙스버그에 소재한 버지니아 주립대학교 110명의 학생들을 대상으로 설문조사를 이용한 양적연구를 실시하였다. 남녀학생 모두가 소비자로서의 역할을 담당할 수 있는 청바지를 선택하여 상표전환 행동에 대한 설문에 답하게 한 후 t-test와 카이 분석을 실시하였다. 그 결과 20%의 응답자가 품절시 상표전환 행동을 보이는 것을 나타났다. 쇼핑시간과 편의성을 덜 중요하게 여기거나, 강한 상표 충성을 보이고, 의류 전문 매장이나 백화점을 주로 이용하는 여성 소비자들의 경우 상표전환을 쉽게 하지 않는 것으로 나타났다. 연구 결과를 바탕으로 의류제조업자 및 소매업자의 제품입고에 관한 제언을 하였다.

Inhibitory Effect of 1-O-Hexyl-2,3,5-Trimethylhydroquinone on Dimethylnitrosamine-induced Liver Fibrosis in Male SD Rats

Yu-Ri Jung,Young-Jung Lee,Nam-Jin Lee,Chun-Mai Lin,Jun-Hawn Moon,Hee-Yul Chai,Jong-Koo Kang 한국독성학회 2010 Toxicological Research Vol.26 No.3

Hepatic fibrosis represents the main complication of most chronic liver disorders and, regardless of its etiology, is characterized by excessive deposition of extracellular matrix components. In this study, we examined that 1-O-Hexyl-2,3,5-Trimethylhydroquinone (HTHQ), a potent anti-oxidative agent, could prevent experimental hepatic fibrosis induced by dimethylnitrosamine (DMN) in male SD rats. Except for vehicle control group, other groups were induced hepatic fibrosis by intraperitoneal injection with DMN (10 ㎎/㎖/㎏) on 3 consecutive days weekly for 4 weeks. During the same 4 weeks, control and DMN groups were given vehicle and HTHQ 50, 100 and 200 groups were orally administered HTHQ (50, 100, 200 ㎎/㎏ respectively). In HTHQ 100 and 200 groups, relative liver weight and serum chemistry level improved significantly. HTHQ reduced hydroxyproline (p<0.05) and malondialdehyde (p<0.05) level in the liver. Histopathological examination of H&E, Masson’s trichrome stain showed the reduced fibrotic septa in HTHQ 100 and 200 groups. HTHQ administration showed reduced mRNA level of PDGF (Plateletderived growth factor), α-SMA (α-smooth muscle actin) and TGF-β (transforming growth factor-β) than DMN-induced hepetic fibrosis animals in the liver tissue. In this study, we showed that HTHQ improves against DMN-induced liver fibrosis in male SD rats.

A data partitioning method for increasing ensemble diversity of an eSVM-based P300 speller

Lee, Yu-Ri,Kim, Hyoung-Nam Elsevier 2018 Biomedical Signal Processing and Control Vol.39 No.-

<P><B>Abstract</B></P> <P>A P300 speller is a device for typing words by analysing the electroencephalogram (EEG) caused by visual stimuli. Among classifying methods used for the P300 speller, the ensemble of support vector machines (eSVM) is well known for achieving considerable classification accuracy. The eSVM is composed of linear support vector machines trained by each small part of the divided training data. To obtain an ensemble model with good accuracy, it is generally important that each classifier be as accurate and diverse as possible; diverse classifiers have different errors on a dataset. However, the conventional eSVM considers only an accuracy viewpoint of an individual classifier by clustering the homogeneous training data with similar noisy components. With such a viewpoint of diversity, we propose a dataset manipulation method to divide a training dataset into several groups with different characteristics for training each classifier. We reveal that the distance between a letter on which a subject is concentrating, and an intensified line on a visual keyboard, can generate EEG signals with different characteristics in a P300 speller. Based on this property, we partition the training data into groups with the same distance. If each individual SVM is trained using each of these groups, the trained classifiers have the increased diversity. The experimental results of a P300 speller show that the proposed eSVM with higher diversity improves the letter typing speed of the P300 speller. Specifically, the proposed method shows an average of 70% accuracy (verbal communication with the Language Support Program is possible at that level) by repeating the dataset for a single letter only four times.</P> <P><B>Highlights</B></P> <P> <UL> <LI> An ensemble method with increased diversity is applied to a P300 speller to achieve higher accuracy. </LI> <LI> We partition the training data into groups with the same distance to increase diversity. </LI> <LI> The proposed method improves the letter-typing speed in a P300 speller. </LI> </UL> </P>