콩 단백 성분 및 연령이 암컷 흰쥐의 혈장 지질 농도와 인지질 지방산 패턴에 미치는 영향

정은정(Eun-Jung Chung),김수연(Soo-Yeon Kim),김지영(Ji-Young Kim),안지영(Ji-Young Ahn),박정화(Jung-Wha Park),차명화(Myung-Hwa Cha),이양자(Yang-Cha Lee Kim) 한국식품영양과학회 2003 한국식품영양과학회지 Vol.32 No.2

혈중 지질농도는 식이단백질 종류보다는 연령의 영향을 많이 받아, young군보다 old군의 총 콜레스테롤, TG, HDL-콜레스테롤, LDL+VLDL-콜레스테롤 및 AI 모두 높았다. 한편 콩 단백질군에서 HDL-콜레스테롤 수준이 증가하고, LDL+VLDL-콜레스테롤와 AI는 감소하여, 심혈관질환에 대한 콩 단백질의 유익한 효과를 확인할 수 있었다. 식이단백질에 의해 유의한 영향을 받은 혈장 인지질의 지방산조성중 22:0, 18:1ω9, ∑MUFA 조성은 카제인군에서 콩 단백질군 보다 높았으며, 반대로 ∑SFA 조성은 카제인군에서 낮았다. 연령에 의해 혈장 인지질 지방산 조성에 있어 유의한 차이를 보인 지방산 중 22:0, 18:1ω9, 22:1, 18:3ω3 및 22:4ω6는 young군에서 높은 반면에, 22:6ω3, ∑ω3, 18:2ω6, 20:4ω6, ∑ω6 및 ∑PUFA의 조성비율은 old군에서 높게 나타났다. 이와 같이 식이 단백질의 종류보다 연령의 영향력이 더 많이 나타난 것은 혈중 콜레스테롤 수준이 식이 단백질보다는 연령의 영향을 더 크게 받았기 때문으로 생각된다. 혈장 인지질 지방산의 대사지표 중 Δ7-desaturation index(16:0⇒16:1ω7)와 Δ9-desaturation index(18:0⇒18:1ω9)가 식이 단백질의 영향을 받아 카제인군에 비해 콩 단백질군에서 유의하게 낮았다. Δ7- 및 Δ9-desaturation index만이 식이 단백질의 영향을 받은 것은 식이 단백질보다 desaturase 활성에 더 큰 영향력을 미치는 콜레스테롤이 식이에 첨가되었기 때문이라 생각된다. Δ-4 desaturation index (22:4ω6⇒22:5ω6)는 young군에 비해 old군에서 높았으며, elongation index(20:4 ω6⇒22:4ω6)는 old군에서 낮았다. 대부분의 elongation과 desaturation 단계는 연령에 따라 유의한 차이를 보이지 못하였으나, ω3계 지방산의 전체적인 elongation-desaturation 단계를 나타내는 ∑products-fatty acid(ω3)/α-LNA(ω3) 비율이 old군에서 young군보다 유의하게 높아, 연령에 따른 PUFA 대사의 변화가 관찰되었다. 이상에서와 같이, 콩 단백질의 섭취로 흰쥐 혈장 인지질의 ∑MUFA 조성은 낮고 ∑SFA 조성은 높아 다른 지질 강화성분의 섭취와 유사한 결과를 나타내었다. 혈중 콜레스테롤 수준이 높은 old군에서 체내 막조직의 유동성을 유지하기 위해 PUFA의 합성 특히 ω3계 지방산의 elongation-desaturation이 증가하여 총 PUFA 조성이 young군보다 높았다. 연구결과로 이소플라본을 포함한 콩 단백질의 혈중 지질저하 기전에 대해 체계적인 설명은할 수 없지만, 콩 단백질이 혈장 지방산의 조성을 변화시킴으로써 간접적으로 심혈관계 질환에 대해 유익한 효과를 미침을 알 수 있었다. 한편, 이러한 혈관의 건강과 관련된 콩의 유익한 효과는 이소플라본과 같은 콩의 특정 성분의 단독효과라기보다는 콩 단백질과의 복합효과에 의한 것이라는 주장은 매우 설득력 있다고 생각된다. Effects of soy protein concentrate (SPC) containing isoflavone and casein diets on plasma phospholipid (PLs)-fatty acid patterns were investigated in 7- & 40- wk old female rats. Diets containing 16% SPC (soy/young: SY, soy/old: SO) and casein (casein/young: CY, casein/ old: CO) supplemented with 0.5% cholesterol were fed for 4 wks. Fatty acid compositions of plasma PLs were determined by TLC and GLC. Compared to the dietary protein effects, age effects on serum lipids were more profound. The levels of total cholesterol (Chol.), triglyceride, HDL-Chol., (LDL+VLDL)-Chol. and atherogenic index (AI) were higher in older groups (OC & OS) than younger groups (YC & YS). Soy groups had higher HDL-Chol. level and lower (LDL+VLDL)-Chol. and AI, compared with casein groups. The compositions of C22:0, C18:1ω9 and sum of MUFA in plasma PLs were significantly higher in casein group (CY & CO) than soy group (SY & SO), but those of sum of SFA were higher in soy group. The compositions of C22:0, C18:1ω9, C22:1, C18:3ω3 and C22:4ω6 were higher and those of C22:6ω3, sum ofω3, C18:2ω6 C20:4ω6, sum ofω6 and sum of PUFA were lower in plasma PLs of younger rats. The average P/S and ω3/ω6 ratio in older group was higher. The Δ-7 desaturation index (16:0⇒16:1ω7) and Δ-9 desaturation index (18:0⇒18 : 1ω9) were lower in soy group than casein group, while Δ-6 and Δ-5 desaturation index were not affected by dietary protein. The Δ-4 desaturation index (22:4ω6⇒22:5ω6) were higher and, elongation index (20:4ω6⇒22:4ω6) were lower in older group. The ratio of the products of ω3 fatty acid series/precursor of ω3 fatty acid series (C18:3) was significantly higher in older group, which in- dicated that age affected the plasma PUFA metabolism. On the other hand, older rats had higher serum cholesterol level compared with younger rats. Taken together, these changes in fatty acid composition might cause minimal changes in the membrane fluidity induced by the increase serum cholesterol level.

The Association Between Long Working Hours and Infertility

Ahn Joonho,Lee Sang Ha,Park Min Young,Oh Soo Hyun,Lee Wanhyung 한국산업안전보건공단 산업안전보건연구원 2021 Safety and health at work Vol.12 No.4

Background: This study aimed to investigate whether working long hours was related to infertility among female Korean workers, while taking age into consideration. Methods: We used data from the 2018 National Survey on Fertility and Family Health and Welfare in Korea that is a cross-sectional, nationally representative, and population-based survey. Infertility was defined as women who were not pregnant after regular unprotected intercourse for a year. Working long hours was classified as ≥52 hours, and subgroups as per age were classified on the basis of being younger or older than 40 years of age. Differences in infertility risk between the long working hour group and none were estimated in crude and fully adjusted logistic regression models with age-group stratification. Results: Of 5,909 Korean female workers, the crude and adjusted odds ratios (95% confidence intervals) of infertility for working long hours were 1.295 (0.948-1.737) and 1.303 (0.921-1.809), respectively. In the subgroup of patients below 40 years of age, the crude and adjusted odds ratios (95% confidence interval) were 1.957 (1.216-3.039) and 1.921 (1.144-3.120), whereas those aged 40 years or older had 0.994 (0.647-1.471) and 0.939 (0.560-1.501), respectively. The weighted prevalence of infertility increased as weekly working hours increased only for the younger than 40-year subgroup. Conclusions: Infertility is associated with working long hours, especially in young-aged workers. Thus, the working schedule must be structured to better suit young female workers.

만성 B형 간염에서 라미부딘 치료중 발생한 Viral Breakthrough 예의 임상 결과

안수현,장윤정,오성남,최도원,백수정,정원석,최창원,김경오,임형준,조남영,박종재,김재선,박영태,이명석,연종은,변관수,이창홍 대한간학회 2002 Clinical and Molecular Hepatology(대한간학회지) Vol.8 No.4

목적: 만성 B형 간염의 치료 중 발생하는 약제 내성 변이종은 임상적으로는 치료 중 음전되었던 혈청 HBV DNA가 다시 양전되는 viral breakthrough 로 진단할 수 있다. 현재 약제 내성 변이종이 발생했을 경우라도 라미부딘 치료를 계속 유지하는 것을 권장하고 있으나, viral breakthrough 발생 예들의 장기적 임상경과가 아직도 불명확하여 이것을 일반화하기는 어려운 상황이다. 이에 라미부딘 사용 중 viral breakthrough 가 발생한 예들을 대상으로 그 임상경과를 알아보고자 하였다. 대상과 방법: 9개월 이상 라미부딘을 투약한 만성 B형 간염 환자로 viral breakthrough가 발생한 74명을 대상으로 하였다(남/여 54/20, 평균연령 42세). Viral breakthrough 후 혈청 ALT치, 총 빌리루빈치, HBV DNA 역가, HBeAg, anti-HBe를 정기적으로 검사하면서 임상경과를 관찰하였다. Viral breakthrough 후 라미부딘의 투약기간은 평균 13개 월(1-41개월)이었다. 결과: Viral breakthrough 발생후 혈청 ALT치가 정상으로 유지되었던 환자는 8예(11%)에 불과했고 나머지 66예(89%)에서는 ALT치가 증가하였으며, 이중 30예(41%)에서 급성 악화(ALT 정상 상한치의 5배 이상 상승)를 보였다. 급성악화는 viral breakthrough 후 3개월 내에 19예 (63%)에서 발생하여 3개월 내에 발생한 예가 많았으나 12개월 이상 지나서 나타나는 예도 약 20%에서 있었다. 비대상성 악화는 6예에서 관찰되었다. Viral breakthrough 후 급성악화가 일어난 예와 없었던 예의 비교에서 급성악화를 예측할 수 있는 인자는 발견할 수 없었다. Viral breakthrough 후 발견할 수 없었다. Viral breakthrough 후 HBeAg이 음전된 예는 8예(11%)였으나 그 임상경과는 양호하지 않았다. 결론: 만성 B형 간염 환자 에서 라미부딘 투여 중 발생한 viral breakthrough 예 중 상당수에서 급성악화가 발생하였으며, HBeAg 이 소실되더라도 그 임상경과는 양호하지만은 않았다. Viral breakthrough 발생 후 주의 깊은 임상경과 의 관찰이 요구되며, 앞으로 viral breakthrough 후 급성악화 예에 대한 대규모 연구와 적절한 치료방향의 제시가 이루어져야 할 것으로 생각된다. Background/Aims: Long-term lamivudine therapy can induce the emergence of lamivudine resistant hepatitis B virus (HBV) mutants. Clinically emergence of the mutant is expressed by the reappearance of disappeared HBV DNA in serum. Continued lamivudine treatment has been usually recommended in cases of viral breakthrough. However, the clinical outcome in patients with viral breakthrough is not clear. The aim of this study was to investigate the clinical course of chronic hepatitis B patients after viral breakthrough during lamivudine therapy. Methods: A total of 74 patients with chronic hepatitis B who showed viral breakthrough after at least 6 months of lamivudine treatment were included in this study. They had positive HBeAg and HBV DNA before treatment. The median follow-up duration after breakthrough was 13 months. Results: After viral breakthrough, only 8 patients (11%) maintained normal ALT levels and 66 patients (89%) showed elevation of ALT. 30 patients (41%) showed acute exacerbation of hepatitis (ALT increase over five-times upper normal limit). These acute exacerbations occurred within three months after breakthrough in 19 patients (63%). In the cases of acute exacerbation, 6 patients showed decompensated progression such as elevation of serum total bilirubin. One of them died of hepatic failure. A predictive factor for acute exacerbation was not found. HBeAg seroconversion occurred in 8 patients after viral breakthrough but their clinical course was highly variable. Conclusions: Chronic hepatitis B patients who had viral breakthrough during lamivudine therapy should be followed carefully and regularly in mind of potential clinical deterioration. New strategies are needed to manage the cases of acute exacerbation after viral breakthrough.(Korean J Hepatol 2002;8:389-396)

교근과 이하선 및 하악골에 발생한 횡문근 육종의 치험례

정수일,박재범,이계영,김도균,안재진 大韓顎顔面成形再建外科學會 1998 Maxillofacial Plastic Reconstructive Surgery Vol.20 No.1

자체 유도 현상에 대한 직관적 사고의 내용과 원인 분석을 통한 과학자적 이해

안수영 한국과학교육학회 2003 한국과학교육학회지 Vol.23 No.2

자체 유도 현상은 고등학교의 모든 물리Ⅱ 교과서에서 다뤄지고 있는 중요한 개념이다. 자체 유도 현상에 대한 설문 조사 결과, 코일로 구성된 회로에서 스위치를 열기만 하면 고압의 유도 기전력이 발생한다는 과 일반화된 사고는 일반고등학교 학생뿐만 아니라 과학고등학교 학생들에서도 심각할 정도로 많이 나타난다. 자체 유도 현상에 대한 학생들의 일반화된 사고의 원인을 현행 물리Ⅱ 교과서 분석을 통해 찾아볼 수 있었다. 그 결과, 특정 회로에서의 네온 전구의 점등 현상을 바탕으로 학생들의 직관적 사고에 의존한 막연한 설명이 주된 원인으로 나타난다. 자체 유도 현상에 대한 과학자적 이해를 돕기 위하여 정량적 해석을 시도하였으며, 이를 바탕으로 학생들에게 자체 유도 현상을 효과적으로 이해시킬 수 있는 정성적인 접근 방법을 찾아보았다. Self-induction is and important concept in the field of electromagnetism, which is dealt with in all the high school physics textbooks. According to the results of the survey on self-induction, most of general high school students and even science high school students have overgeneralized concepts that high self-induced emf. are produced whenever the switch is turned off in the circuit containing inductors. The reasons of this overgeneralization on self-induction could be explained through the analysis of current high school physics Ⅱ textbooks. Main reasons can be attributed to the fact that, by depending on their intuitive ideas. students try to vaguely explain the concept, based on lighting up of Ne tube in the special circuit. This study found out qualitative method to have students effectively understand self-inductions based on quantitative interpretation to gain scientific understanding ong self-induction.

가토 전방내 혈액흡수에 미치는 Aspirin의 효과에 관한 실험적 연구

안영순,이태수 고려대학교 의과대학 1982 고려대 의대 잡지 Vol.19 No.3

In ocular diseases, hyphema is often experienced. Clinically there are many serious complications such as glaucoma, cataract, blood stain to cornea, etc. Recently, the author has been interested in aspirin which has inhibited action of platelet aggregation and prolongation of bleeding time when it is used systemically. But everyone don't know what influence on the absorption of the anterior chamber hemorrhages when we use it as the subconjunctival or intracameral routes. In this study, simulated anterior chamber hemorrhages were produced by intracameral injection of 0.1㏄ autogenous ^(51)Cr labeled blood through the limbus in 28 rabbits eyes. Thereafter, the author treated the rabbits with aspirin by subconjunctival (40㎎/0.2 ㏄), intracameral(20㎎/0.1㏄), and intramuscular injection (200㎎/㏄) everyday for 10 day. All experimental groups were measured and recorded the changes of radioactivity of the eyes with scintillation counter for 10 days. The final results were summarized as follows ; 1) The time required for 50% of the injected radioactivity to disappear after experimental hyphema was 18 hours in the intracameral injection group, within 48 hours in subconjunctival injection group, over 72 hours in both control and intramuscular infection groups. 2) The rates of removal of the radioactivity from the anterior chamber didn't make any difference between control and intramuscular injection groups. 3) In general, the intracameral and subconjunctival injection groups showed faster rate of absorption of blood from the anterior chamber than the other two groups.

Monocrotaline으로 유발시킨 흰쥐 폐고혈압에 대한 Flunarizine의 약리작용

안형수,이영미 同德女子大學校 1997 同大論叢 Vol.27 No.1

Monocrotaline treatment (60mg/kg, i.p.) in normotensive rats resulted the indications and related toxicities of pulmonary hypertension, including the increase of hematocrit value, right ventricle hypertropy, pulmonary edema and lethality, after 4 weeks later. However, daily administrtion of flunarizine (5mg/kg, p.o.) for 4 weeks seemed to decrease the above responxes, and also there was no significant change on the systemic mean arterial blood pressure and heart rate. In the anesthetized rats, hypotensive effect induced by isoproterenol bolus injection was signficantly inhibited in flunarizine-treated pulmonary hypertensive rats, comparing to the untreated rats, even if those by acetylcholine, adenosine and ATP were not. In the isolated pulmonary artery experiments, vasorelaxation induced by isoproterenol, but not acetylcholine, adenosine or ATP, were also selectively inhibited in flunarizine treated rats, comparing to the control rats. Also vasoconstriction induced by norepinephrine were increased in flunarizine treated rats comparing to the control rats, but those by KCI were not. However, pretreatment of flunarizine did not significantly affected on the vasodilation induced by acetylcholine, isoproterenol, adenosine or ATP and on the vasoconstriction induced by norepinephrine in isolated pulmonary artery of the normal rats. These results suggest that flunarizine will decrease the pathogenesis of pulmonary hypertension through the change of α- and β-adrenergic receptor activity in pulmonary artery due to the up-regulation of voltage dependent Ca++ channels.

세기변조방사선치료의 선량분포 검증에 관한 연구

권수일,신동호,송재영,안진호 京機大學校 2006 論文集 Vol.50 No.-

Film dosimetry as a part of patient specific intensity modulated radiation therapy quality assurance (IMRT QA) has been accomplished to develop the new optimization method of film isocenter offset and to suggest the new quantitative criteria for film doimetry after optimization. Five patients with head and neck cancers were measured in IMRT dosimetry by film method. Optimization method with the local minimum was developed to adjust the film isocenter offset error, which is the biggest part of systemic errors. The mean ratios were 5.33% and 7.09% for 5mm and 1 mm resolution dose data optimization respectively.

大學生의 써클활동에 관한 分析的 硏究

安桂洙,金赫坤,鄭映珍 순천대학 학생생활연구소 1985 學生生活硏究 Vol.4 No.-

1930년대 영국 문인들의 이념적 갈등

안영수 慶熙大學校 1990 論文集 Vol.19 No.-

The decade of the 1930s was a time of crises for Europeans. Particularly, the British intellectuals had experienced a tremendous shift of thinking. The predominant political attitude of the young writers at this time had been liberal or left-wing. Communism was more than fashionable: it represented the logical point of opposition to Fascism in Germany and Italy, and the Spanish Civil War provided a stage on which to rehearse the approaching conflict. The group of young poets of this period was called "the Auden Generation," the members of which consisted of W. H. Auden, Stephen Spender, C. Day Lewis, Louis MacNeice, etc. The "political consciousness" was growing so much that they believed art would become a weapon in the class struggle, and the poet, in a way more immediated than ever before, would be 'an unacknowledged legislator of the world,' The major writings of this generation can all be understood as efforts to deal with the crucial problems of the times. But at the end of the decade, the aspiration of the Auden generation turned out to be a mere fantasy. They realized that their dream of solving social problems by means of literature failed; they were self-deceived out of false hope and ineffectual indignation against the established order of society. They were left behind with the sense of disillusionment. They gradually with drew from the ideological commitments and became more concerned with aesthetic values of literature. They have found out that literature and politics could not go together. Auden, the boss of "the Auden Generation" emigrated to the United Sates in January 1939, a symbolic gesture of his defeat in the aforementioned struggle. The one-time active Communist-poet Day Lewis and Spender retreated into an ivory tower of literature wherein they sang individual lots of human suffering and the need of mutual understanding and love amongst mankind. And MacNeice, who wrote more personal, nostalgic poetry and could not settle in one place, became a self-exiled poet in the western world. In sum, the Myth of the Thirties was an "unmitigated failure."