호흡기 알레르기 질환을 가진 소아에서 최근 10년간 흡입항원 감작의 변화

김영진,한지은,강임주 대한알레르기학회 2004 천식 및 알레르기 Vol.24 No.2

녹즙의 위생화를 위한 비가열 살균기술 연구

김영지 영남이공대학 산업기술연구소 2000 産業技術硏究 Vol.12 No.-

The comparative effects of ozone treatment and gamma irradiation on the sterilization and physicochemical properties of the fresh vegetable-extract juices were investigated during the storage periods at 4℃. As for the sterilzation of microorganisms, the viable cells of 3~5 kGy irradiated fresh vegetable-extract juices were detected below the level of 10^5 CFU/㎖ during 12days at 4℃. On the other hand ozone treatment failed to eliminate the highly contaminated microbial load, especially total aerobic bacteria. The physicochemical properties including ascorbic acid, chlorophyll and carotenoid contents, electron donation ability, and peroxidase activity were not significantly changed by gamma irradiation up to 5 kGy, whereas ozone treatment caused significant changes in destrution of natural pigments and ascorbic acid (P<0.05). The above results led us to conclude that gamma irradiation was more effective than ozone treatment for the sterilization and maintenance of physicochemical qualities of fresh vegetable-extract juices.

대구지역 여대생들의 신체상에 따른 식행동 요인 분석 : Dietary restraint, Disinhibition and Hunger

김주남,김영지,이난희 영남이공대학 2000 論文集 Vol.29 No.-

In order to assess relationships between body image and eating behavior scores from the Three Factor Eating Questionnaire(dietary restraint, disinhibition, hunger), interviews were done on 181 foliate college students in Daegu area Among the students, underweight students were 59(32.6%), normal weight students were 104(57.5%) and obese students were 18(9.9%) by BMI standard. Body image and eating behavior scores were compared among underweight, normal, and obesity using one-way analysis of variance. The Pearson correlation coefficient was used to evaluate relationships. Relationship of Dietary restraint score and disinhibition score with groups(underweight, normal, obesity) were correlated(p<0.001, p<0.01, respectively). Statistically significant correlations were obtained between the respondent's current weight and both Dietary restraint score(p<0.01) and disinhibition score(p<0.05) : the correlation coefficients were respectively, 0.324, 0.165. College students are in early stage of adult life and their dietary attitudes, food behaviors and life style will continue to their later life. Therefore college students need more positive dietary attitudes to prevent and treat students obesity.

떫은감 탄닌성분이 초산발효에 미치는 영향

서지형,김영지,김주남 영남이공대학 산업기술연구소 2002 産業技術硏究 Vol.14 No.-

To improve the quality of persimmon vinegars, this study was investigated effects of persimmon tannin on acetic acid fermentation. During fermentation sugar contents didn't changed in all groups. Total acidity of 0.15 % tannin group was very low, only 2.5 %. Browning reaction during acetic acid fermentation increased continuously. Especially the color values and turbidity of 0.15 % tannin group were different from other groups. The contents of total phenolic materials and tannin decreased during acetic acid fermentation.

유아용 시럽형 약물이 구강 내 산도에 미치는 영향

안지영,김재문,정태성,김신 大韓小兒齒科學會 2007 大韓小兒齒科學會誌 Vol.34 No.4

Syrup form medicines which children commonly take contains sugar as sweeteners. Sugar, low endogenous pH, high acidity, mucosity and low concentrations of ions including those of calcium, fluoride, and phosphate in their composition, they can be cause of caries and erosion. Not only the properties found in such medicines. but also other factors such as high frequency of ingestion, bedtime consumption, and the collateral effect of a reduction in salivary flow, poor oral hygiene of sick children may also contribute to the risk. The other side, parents rarely recognize these risk, and medical experts also easily overlook. The purpose of this study was to investigate the pH level of some syrup form medicines which are frequently administered by infants and young children, and their effects on the changes of plaque pH when rinsing with them. And we compared the salivary pH change induced by rinsing with Cough-syrup only and rinsing with Cough-syrup followed by water. The results were as follows : 1. The average pH of syrup form medicines which were prescribed in Pusan National University Hospital and several OTC syrup form medicines was pH 4.7±0.94, within the range of pH 3.0 to 6.8. 2. The plaque pH decreased rapidly below pH 5.5 after rinsing with the syrup form medicines which were selected for the test and there was no statistically significant difference in the of plaque pH change between syrup form medicines except C0LDI(p>0.05). 3. There was statistically significant difference in salivary pH change between rinsing with Cough-syrup only and rinsing with water after Cough-syrup(p<0.05). 유아용 시럽형 약물은 어린이가 쉽게 복용할 수 있도록 여러 종류의 당분을 감미제로 사용하고 있으며 그로 인한 우식의 위험이 높다고 할 수 있다. 그러나 시럽형 약물의 우식유발성에 대한 보호자의 인지도는 매우 낮으며 의료 종사자들 조차 이를 소홀히 여기고 있어 문제의 심각성이 크다고 할 수 있다. 본 연구는 시럽형 약물이 구강 내 산도에 미치는 영향에 대하여 조사할 목적으로, 15 종의 시럽형 약물의 산도를 조사 하고,그 중 널리 시판되는 4종을 선택하여 이 약물들이 치태의 산도 변화에 미치는 영향을 평가하였다. 또한 가장 흔하게 사용하는 유아용 시럽형 약물의 일종인 코푸시럽의 복용방법에 따른 타액의 산도 변화를 측정하여 다음과 같은 결과를 얻었다. 1. 15 종 시럽형 약물의 산도는 pH 3.0∼6.8의 범위에서 다양하여 평균 pH 4.7±O.94로 나타났다. 2. 치태의 산도변화를 측정하기 위해 선정된 4종의 시럽형 약물 모두는 구강 내 투입 직 후 법랑질의 탈회 임계점인 pH 5.5 이하로 치태의 산도를 급격히 떨어뜨렸으며,시간 경과에 따른 치태의 산도 변화에서 콜디(종합 감기약)를 제외한 모든 약물에서는 유의한 차이가 없었다(p>O.05). 3. 코푸 시럽으로 구강을 행구어낸 경우와 코푸 시럽에 이어 물로 구강을 행구어낸 경우 타액의 산도 변화에 있어서는 유의한 차이가 있었다(p<O.05).

중국거주 조선족 여성의 영적 안녕정도가 정신건강에 미치는 영향

정성덕,이종범,김진성,서완석,배대석,박순재,주열,염형욱,김승원,김구묘,안영록,황대홍,표미자,조창열,정태길 영남대학교 의과대학 2004 Yeungnam University Journal of Medicine Vol.21 No.2

자아초월 정신의학이 체계화되면서 영성(spirituality)은 인성의 한 부분이라는 이론이 대두되었는데 이 영성은 정신건강에 큰 영향을 미친다고 했다. 인간의 성향 중 영적 안녕 정도를 파악하여 그 정도가 정신건강 중 허위성 경향, 정신병적 경향 및 불안과 우울 경향 등에 어떠한 영향을 미치는지를 조사하고저 본 연구를 실시하였다. 대상은 중국의 연변 조선족 자치구에 거주하는 여성 400명을 대상으로 하여 한국판 영적 안녕척도를 사용하여 영적 안녕정도를 파악하였다. 영적 안녕척도의 총점과 이 척도의 2개 하위 척도인 종교적 안녕과 실존적 안녕 척도 양자로 평가한 성적이 불안-우울통합척도와 정신분열증 척도 및 허위성 척도로 평가한 성적에 미치는 영향을 검증하기 위하여 상관관계분석 및 회기분석을 실시하였다. 그 결과는 다음과 같다. 1) 영적 안녕척도로 평가한 조선족 여성의 총점은 68.29로 한국의 기독교 여성이 평가한 100.65보다 훨씬 낮은 점수였다. 2) 불안·우울통합척도로 평가한 총점은 44.88로 연변노인이 평가한 점수와 일개지역의 한국농촌주민이 평가한 점수와 비슷한 결과였다. 3) 허위성 척도로 평가한 성적은 평균 74.57로 70점 이상이 86%(344명)이었으나 영적 안녕 성적과 허위성 성적 간에는 유의한 상관이 없었다. 4) 영적 안녕총점은 정신분열증 척도로 평가한 정신병적 경향에 유의한 영향을 미치지 않았으나 종교적 안녕하위척도의 성적은 정신병적 경향을 높여준데 비하여 실존적 안녕하위척도의 성적은 정신병적 경향을 낮게 해주었다. 5) 영적 안녕척도의 총점 및 두 하위척도인종교적 안녕과 실존적 안녕은 불안과 우울에유의한 상관을 보였는데 영적 안녕총점이 높을수록 불안·우울 총점이 다소 낮아지는 경향을 보였다. 6) 종교적 안녕 하위척도와 불안과 우울과의 관계는 종교적 안녕점수가 높을수록 불안과 우울을 각각 다소 유의하게 높여주었으며 이에 비하여 실존적 안녕하위척도와 불안과 우울과의 관계는 실존적 안녕정도가 높을수록 불안 및 우울점수는 유의하게 낮아졌다. 이와 같은 성적을 미루어볼 때 연변에 거주하는 조선족 여성이 평가한 영적 안녕정도는 정신병적 경향과 불안 및 우울에 유의한 상관을 보였으면 이 척도의 하위 척도인 종교적 안녕정도는 정신건강에 부정적인 영향을 미치는 반면 실존적 안녕정도는 정신건강에 긍정적 영향을 미친 결과로 평가되었다. 이러한 결과는 공산주의 사회에서 실존적 안녕은 긍정적인 가치관으로 평가되는 반면 종교적 안녕은 정신건강에 부정적으로 작용한 것을 입증해 주었다고 하겠다. Background: Spirituality has been an important part of Transpersonal Psychology and is believed to have a large effect on the mental health because it has been systematized. The aim of this study was to determine the level of spiritual disposition on human beings along with its effects on one's mental health. Materials and Methods: The study targeted 400 women residing in Youn-Gil city of JiLin Prov., which is a district of the Cho-Sun tribe in China. Their spiritual well-being was studied using the Spiritual Well-being Scale-Korean Version. The spiritual well-being scale consists of 2 sub-scales of religious well-being and existential well-being. The study was evaluated using a lie scale, psychotic trend, and a combined anxiety-depression scale. The results were considered to be factors of one's mental health. The correlation between the spiritual well-being and each tendency was analyzed by regression analysis. Results: The total score of the Cho-Sun tribal women according to the spiritual well-being scale was 68.29 which was much less than the 100.65 of Korean Christian women. There was no significant correlation between the spiritual well-being and the Lie trend. However, it was found that 86%(344) of Cho-Sun tribal women scored above 70 in the Lie trend with a mean score of 74.57 which is higher than normal populations. Regarding the correlation between the spiritual well-being and psychotic trend, the psychotic trend became significantly higher when the religious well-being was at a high level. On the other hand, the psychotic trend became significantly lower when the existential well-being was at a high level. Regarding the correlation between the spiritual well-being and anxiety, the anxiety was significantly higher when the religious well-being was at a high level. However, the anxiety level was significantly low when the existential well-being was at a high level. Regarding the correlation between the spiritual well-being and depression, the depression level was somewhat significantly high when the religious well-being was at a high level. However, the depression level was significantly low when the existential well-being was at a high level. Conclusion: This study evaluated the effects of spiritual well-being on a person's mental health among Cho-Sun tribal women in Youn-Gil city of JiLIn Prov., P.R. of China. The results found that the religious well-being, which is a sub-scale of spiritual well-being, had negative effects while the existential well-being had positive effects on the mental health. These results proved that a person's religious disposition had negative effects on their mental health in a communitarian society.

오미자와 산수유 추출물이 dihydrotestosterone가 처리된 LNCaP 인간 전립선 암세포의 증식 및 전립선 비대 유발 인자 발현에 미치는 영향

김민영(Min Yeung Kim),지선영(Seon Yeong Ji),황보현(Hyun Hwangbo),이혜숙(Hyesook Lee),김태희(Tae Hee Kim),윤선혜(Seonhye Yoon),김현진(Hyun Jin Kim),김성연(Sung Yeon Kim),김태중(Tae Jung Kim),김민지(Min Ji Kim),정하은(Ha Eun Jung),최영현( 한국생명과학회 2021 생명과학회지 Vol.31 No.10

오미자와 산수유는 한국을 포함한 동아시아 지역에서 다양한 질병의 예방 및 치료에 오랫동안 사용되어 왔다. 최근에 이들 추출물에 의한 양성 전립선 비대증(BPH)의 발병 및 진행을 억제할 수 있다는 가능성에 대한 보고가 있었지만 관련 기전에 대한 연구는 여전히 부족한 실정이다. 본 연구에서는 LNCaP 전립선 세포를 사용하여 DHT 처리에 의한 in vitro BPH 모델에서 오미자 및 산수유 추출물에 의한 BPH의 개선 가능성을 조사하였다. 본 연구의 결과에 의하면, 오미자와 산수유의 열수 및 에탄올 추출물은 DHT 처리에 의해 LNCaP 세포의 증식을 유의적으로 억제하였으며, DHT로 유도된 BPH 바이오 마커와 성장인자의 발현을 현저히 감소시켰다. 그들은 또한 세포사멸 관련 인자의 발현을 조절하였고, DHT 매개 산화적 스트레스를 유의적으로 감소시켰으며, BPH 발병에 관여하는 주요 인자에 대한 보호 효과는 열수 추출물보다 에탄올 추출물 처리군에서 더 효과적이었다. 또한, BPH에 대한 보호 효과는 오미자와 산수유의 에탄올 추출물 단독 처리군보다 1:1 복합 혼합물 처리군에서 더 높았으며, 60% 에탄올 추출물이 40% 에탄올 추출물보다 더 높은 개선 효과를 보였다. 따라서 본 연구 결과는 오미자와 산수유 추출물이 항산화 활성과 연관된 androgen 신호 전달 경로의 억제를 통해 전립선 세포의 과다 증식을 방지함으로써 BPH 개선에 관여할 수 있음을 의미한다. 따라서 오미자와 산수유 추출물은 BPH의 임상 치료에 유용할 수 있으며, 이 두 추출물의 조합은 BPH 개선에 상승 효과를 낼 수 있을 것이다. Schisandrae Fructus (SF) and Corni Fructus (CF) have been used for a long time for the prevention and treatment of various diseases. Although reports have highlighted the possibility of inhibiting the onset and progression of benign prostatic hyperplasia (BPH), studies on related mechanisms are still lacking. In this study, we investigated the potential of SF and CF in improving BPH by using a dihydrotestosterone (DHT)-induced in vitro BPH model using LNCaP prostate carcinoma cells. According to our results, water and ethanol extracts of SF and CF significantly inhibited the proliferation of LNCaP cells by DHT treatment and markedly downregulated the expression of DHT-induced BPH biomarkers and growth factors. They also regulated the expression of apoptosis regulatory factors and significantly reduced DHT-mediated oxidative stress. In addition, the protective effect on major factors involved in the pathogenesis of BPH was more effective in the ethanol extract treatment group than in the water extract group. Furthermore, the improvement effect on BPH was higher in the 1:1 combined treatment group than in the ethanol extract alone treatment group of SF and CF, and 60% ethanol extracts showed a better effect than 40% ethanol extracts. Therefore, our findings demonstrate that SF and CF can protect against BPH by preventing the hyperproliferation of prostate cells through the inhibition of the androgen signaling pathway, which was correlated with their antioxidant activities. Therefore, SF and CF extracts may be useful in the clinical treatment of BPH, and the combination of these two extracts can be synergistic.

Slide Session : OS-HEM-11 ; Hematology : Impact of Stem Cell Dose and Performance Status on Cord Blood Transplantation (CBT) Outcome in Adult Patients with Advanced Hematologic Diseases: 13-Year Experience in Korea

( Ji Won Kim ),( Junglim Lee ),( So Young Chong ),( Chul Won Jung ),( Yeo Kyeoung Kim ),( Yeung Chul Mun ),( Deog Yeon Jo ),( Hong Ghi Lee ),( Jong Ho Won ),( Joon Seong Park ),( Jae Hoon Lee ),( Joon 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

Background: CBT is an established alternative donor transplantation for treatment of patients with advanced hematologic diseases. Methods: To assess clinical outcome after CBT and provide future recommendations for alternative donor, we conducted the first retrospective multicenter study of adult CBT in Korea. Results: Between June 2001 and April 2014, 48 patients (male, 19) were enrolled from 16 hospitals. The median age was 36 years (range, 16-65 years). Forty patients (83.3%) were acute leukemia: 33 (68.8%) in remission and 7 (14.6%) in persistence. The others were myelodysplastic syndrome (n=3, 6.3%), severe aplastic anemia (n=3, 6.3%), and lymphoma (n=2, 4.2%). Thirty two patients (66.7%) underwent double unit CBT. Twenty two patients (45.8%) underwent myeloablative conditioning and 26 patients (54.2%), reduced-intensity conditioning. Total body irradiation (TBI)- based conditioning regimens were used in 23 patients (47.9%). The median time to neutrophil >500/μL was 20 days among grafted, and 14 patients (29.2%) failed to recover. During follow-up, 8 patients died of disease and non-relapse mortality (NRM) developed in 23 patients. The 2-year overall survival (OS) was 29.6%. In multivariable analysis, ECOG performance status (PS) =2 (hazard ratio [HR] 6.42, p=0.001) and total nucleated cell dose <3.0x10^7/kg (HR 2.59, p=0.012) were poor prognostic factors for OS. Factors associated with graft failure were ECOG PS 2 (p=0.012) and non-TBIbased conditioning (p=0.026). CBT outcomes after January 2009 were significantly improved compared to those before January 2009 (p=0.012). Conclusions: Although in the past, the clinical outcome of adult CBT was not satisfactory in Korea because of high NRM, it has been improved in recent years. Our data suggest that the clinical outcome substantially depends on the cord blood cell dose and PS.

Effects of the Peroxisome Proliferator-Activated Receptor γ Ligand Troglitazone in Osteosarcoma Cell Lines

Yeung Jin Kim(김영진),Tae Kyun Kim(김태균),Jin Young Park(박진영),Hyoung Joon Kim(김형준),Ji Wan Lee(이지완) 대한정형외과학회 2005 대한정형외과학회지 Vol.40 No.5

목적: U2OS와 HOS 골육종 세포주에 대한 Troglitazone (TRO)의 영향을 알아보고자 하였다. 대상 및 방법: TRO를 처리한 골육종 세포의 생활력은 trypan blue exclusion을 이용하여 측정하였고, 세포주기 분포는 흐름세포측정을 이용하여 판정하였다. 세포자멸사는 흐름세포측정과 TUNEL 분석법을 이용하여 측정하였다. 세포자멸사와 세포 주기에 관련된 유전자의 발현은 PTEN, tAkt, pAKt, Bcl-2, Bax, pRE, p21CiP1 and β-actin을 이용한 웨스턴 블롯으로 평가하였다. 결과: U20S와 HOS 세포주 모두에서 세포 성장의 억제를 보였고, 흐름세포측정에서도 모두에서 G1기에서 세포주기 정지가 관찰되었다. 또한 P21cip1과 PTEN 발현은 증가하였고 pRb와 pAKt의 발현은 감소하였다. 그러나 흐름세포측정과 TUNEL 분석법을 이용하여 세포자멸사를 측정한 결과 서로 다른 결과를 보였다. HOS 세포주의 세포자멸시는 증가한 반면 U2OS 세포주의 세포자멸사는 감소하였다. U2OS 세포주에서 세포자멸사의 감소는 Bcl-2 발현의 증가 및 Bax 발현의 감소와 함께 관찰되었다. 결론: TRO가 작용하는 기전이 세포주에 따라 차이가 있을 수 있으나 적절하게 입증된 용량으로 사용한다면 골육종 치료에 이용될 수 있는 가능성을 보여주는 것으로 생각된다. Purpose: We wanted to investigate the effects of Troglitazone (TRO) on the cellular growth in two human osteosarcoma cell lines (U20S and HOS). Materials and Methods: Cell viability was assessed by performing trypan blue exclusion assay. FAC-Scan analysis was performed to study the cell cycle and apoptosis. Antibodies against PTEN, tAkt, pAKt, Bcl-2, Bax, pRB, p21<SUP>CiP1</SUP> and β-actin were used in the Western blot analysis. Results: TRO inhibited the growth of both the osteosarcoma cell lines. TRO induced G0/G1 arrest in the cell cycle progression for both osteosarcoma cell lines. TRO induced the apoptosis of HOS cells, but it decreased apoptosis of the U20S cells. TRO induced Rb dephosphorylation and the increased expression of p21<SUP>CiP1</SUP> TRO increased the PTEN and Bcl-2 expressions and it decreased the level of pAkt, pRb and Bax. Conclusion: The present study suggested that TRO may be used as a chemotherapeutic agent for the treatment of human OS. Yet further study is required for uncovering the precise the mechanism of TRO.

Relationship of Specific Microbial Growth and TBARS Value in Radiation-Sterilized Raw Ground Pork

Kim, Jae-Kyung,Jo, Cheo-Run,Kim, Hyun-Joo,Lee, Kyong-Haeng,Kim, Yeung-Ji,Byun, Myung-Woo The Korean Society of Food Science and Nutrition 2004 Preventive Nutrition and Food Science Vol.9 No.4

Sterilized raw ground pork was inoculated with Pseudomonas aeruginosa (PA) and Lactobacillus casei (LC) to investigate the relationship between microbial growth and 2-thiobarbituric acid reactive substances (TBARS) values. The analyses including microbial growth, pH, and TBARS values were performed during 3 weeks of storage at room temperature $(20^{\circ}C)$. The radiation-sterilized control sample did not show any microbial growth, but the samples inoculated at different levels (diluted twice vs non-diluted) exhibited differences until 1 week. However, the difference disappeared at weeks 2 and 3. The pH of raw ground pork inoculated with PA increased, but that of LC decreased. The pH of non-inoculated samples increased slightly after storage. The TBARS values in non-inoculated and LC inoculated with pork increased, but TBARS remained unchanged in samples inoculated with PA after 1 week. Results indicated that the microbial growth level and strains can influence the TBARS value of raw ground pork. Thus, it is important to use samples exposed to the same microbial conditions to compare the oxidation of lipids in meat samples.