Endothelial Cells Isolated from the Bovine Corpus Luteum Synthesize Prostaglandin F<SUB>2α</SUB> Receptor

Sun?Yeong Gwon,Ki?Jong Rhee,Seunghyung Lee 대한의생명과학회 2013 Biomedical Science Letters Vol.19 No.3

The corpus luteum is a transient endocrine gland essential for regulation of the ovarian cycle as well as for establishing and maintaining pregnancy. Prostaglandin F2α (PGF) initiates functional and structural regression of the corpus luteum and therefore is an important regulator of the estrous cycle. It is a matter of debate whether the endothelial cells of the bovine corpus luteum express PGFR, the cognate receptor for PGF. Therefore, the aim of this study was to assess the expression of PGFR in bovine endothelial cells. Endothelial cells were isolated from the bovine corpus luteum of the mid?luteal stage using magnetic beads and cultured in vitro. We demonstrate that this isolation procedure generates a pure culture of endothelial cells as confirmed by synthesis of Factor VIII and lack of expression of 3β?hydroxysteroid dehydrogenase. By RT?PCR, Western blot and immunofluorescence analyses, we further show that the cultured endothelial cells produced PGFR. This model system can be utilized to provide an experimental system to investigate the role of PGF on endothelial cells during the reproductive cycle.

변색 실활치에 대한 carbamide peroxide gel의 표백효과

박선아,김선호,황윤찬,오병주,윤창,박영준,정선와,황인남,오원만 大韓齒科保存學會 2002 Restorative Dentistry & Endodontics Vol.27 No.4

The bleaching of discolored nonvital teeth is conservative treatement that satisfy the cosmetic desire. The most common method for this treatement, walking bleaching, is using 30% hydrogen peroxide and sodium perborate. Many alternatives are suggested for preventing the external cervical root resorption that is the common complication of the nonvital teeth bleaching with 30% hydrogen peroxide. The same extent of oxidation reactions as that resulted by the bleaching with the application of 30% hydrogen peroxide and sodium perborate can also be acquired more safely by materials that contain 10% carbamide peroxide, used primarily for the bleaching of vital teeth. Therefore, this study was performed to evaluate the efficacy of 10% and 15% carbamide peroxide bleaching gel in nonvatal teeth bleaching. The internal bleaching of intentionally discolored teeth was performed in vitro with 10% carbamide peroxide (Group 1), 15% carbamide peroxide (Group 2), mixture of distilled water and sodium perborate (Group 3), and mixture of 30% hydrogen peroxide and sodium perborate (Group 4). The bleaching materials were refreshed following 3, 6, 9 and 12 days. To evaluate the bleaching effect, the color change of the crowns was measured at 1, 2, 3, 4, 7 and 15 days of bleaching using the colorimeter. The results were as follows : 1. L^* and ΔE^* values were increased with time in all bleaching agents(p<0.01). 2. There was no significant difference in L^* and ΔE^* value among bleaching agents. 3. Δ^* value higher than 3 was shown after 3 days of bleaching with 10% carbamide peroxide gel, 1 day with 15% carbamide peroxide gel, 4 days with mixture sodium perborate and distilled water and 4 days with mixture sodium perborate and 30% hydrogen peroxide, respectively. These results revealed that the use of 10% and 15% carbamide peroxide bleaching gel in non-vital teeth bleaching is as effective as mixture of distilled water and sodium perborate and mixture of 30% hydrogen peroxide and sodium perborate. Accordingly, carbamide peroxide could be used clinically to bleach discolored non-vital teeth.

외음부의 기저세포암

김보영 ( Bo Young Kim ),황선정 ( Sun Jung Hwang ),박병준 ( Byung Joon Park ),이은실 ( Eun Sil Lee ),한호섭 ( Ho Suap Han ),김용욱 ( Yong Wook Kim ),김태응 ( Tae Eung Kim ),노덕영 ( Duck Yeong Ro ),이안희 ( An Hi Lee ) 대한산부인과학회 2010 Obstetrics & Gynecology Science Vol.53 No.9

Basal cell carcinoma (BCC) is the most common human malignant neoplasm, accounting for 75% of all non-melanoma skin cancer. The incidence of BCC is strongly correlated with sun exposure as well as older age. Therefore, the vast majority of BCCs is observed in elderly patients on the sun-exposed skin of the head and neck with a frequency of more than 80%. BCC is very rare on sun-protected skin such as the perianal and genital regions and other etiologic factors must be considered in these cases. Although the pathogenesis of vulvar BCC is unclear, early diagnosis is very important. Because BCC in these areas sometimes seems innocuous, it is recommended that a biopsy of all suspect lesions be performed. We report a woman with BCC of the vulva treated with wide local resection and reviews the literatures on this subject.

한국인의 간과 콩팥조직 내 수은 함유량의 참고치

최병선,박영주,권일훈,홍연표,박정덕 한국환경독성학회 2002 환경독성보건학회지 Vol.17 No.2

This study was conducted to investigate the reference value of mercury (Hg) in liver and kidney of Korean population. The mercury concentration in 244 'sudden and unexpected death' autopsies (male: 180, female: 64) aged from 0 to87 years was analyzed. The concentration of mercury was measured by atomic absorption spec(Perkin -Elmer Model 5100) with mercury/hydride generating system (FIAS 400). The contents of mercury in liver and kidney fitted well the log-normal distribution rather than normal distribution. Geometric mean concentration of mercury in liver and kidney was 0.115 ㎍/g wet weight and 0.149 μg/g wet weight, respectively. Geometric mean concentration of mercury in female was higher than in male (p<0.01). The mercury content in liver and kidney increased with age up to the forties and slightly decreased there-after, The regression model of mercury deposit in liver and kidney by age was predicted as the following equation : Log KHg=-1.0576+0.0045·Age -0.0001·Age²+0.0873·Sex, Log KHg=-1.0576+0.0152·Age-0.0002·Age²+0.1935·Sex. The liver burden of mercury was estimated to be 158.3~161.3μg in male and 163.0-166.9 ㎍ in female. The kidney burden of mercury was estimated to be 42.0~42.9 ㎍ in male and 55.5 ∼57.1 μg in female.

주방용품에 대한 소비자구매 실태조사

이종영,김선희 경북대학교 1992 논문집 Vol.24 No.-

Because of the progress of import liberalization and the rising labor cost, Korean kitchen ware are losing competitive power, like other industries in Korea. More and more the korean consumers are exposed to foreign products and various domestic brands which are competing indomestic kitchen were market. As a result, Korean firms face with more difficult situations. This situation requires Korean firms to adop different marketing strategies. It is necessary that the Korean ware firms adopt cosumer oriented marketing strategy. This study attempts to analize consumer behavior regarding kitchen ware. The main findings of research shows that consumer attitude is drastically changing. That is, there are clear difference between 20∼30 year old housewives and 40∼50 year old housewives in their attitude toward kitchen ware. ◁표 삽입▷ (원문을 참조하세요) The Younger consumers prefers kitchen ware made of stainless. And they prefer department stores, and other one-stop shoppings that have many items in one place. They acquire information from advertising and relatives. They are not so much concerned with high price and use import goods more than older generation. Accordingly, Marketing strategy of kitchen ware will have to decide which segment to target and focus on consumer oriented marketing in that target market.

더덕의 효율적인 재분화 및 뿌리발달

심주선,조숙녀,손화,김무성,노영덕,김세영,양덕춘 경희대학교식량자원개발연구소 2005 硏究論文集 Vol.24 No.-

더덕은 식용, 관상용, 생약재료로 쓰이는 방향성 식물이다. 본 연구는, 종자의 기내배양을 통하여 식물체 재분화와 뿌리발달을 실험했다. 종자의 발아는 GA_(3) 100 mg·L(-1)처리와 저온처리 시 매우 양호하였다. 더덕의 기내에서 shoot의 형성은 2 mg·L^(-1) BA, 2 mg·L(-1) NAA가 첨가된 MS 기본배지에서 가장 양호하였으며, 줄기의 절편체와 비교하여 잎 절편체에서 재분화가 잘되었다. 또한 뿌리의 발육은 계속적인 계대배양에 의해서 양호하였는데 shoot의 길이를 1 cm 정도 남긴 뿌리를 연속적으로 4회 계대배양에서 뿌리가 주근으로 발육하였으며 길이는 약 3 cm 및 직경은 0.5 cm의 정상적인 뿌리를 얻을 수 있었다. Codonopsis lanceolata is widely used as edibles, ornamental plant and crude drug material. This study was carried out to confirm the ratio of germination and efficient differentiation of shoot and root in vitro. The germination of Codonopsis lanceolata seed was excellent in the medium with GA_(3) 100 mg·L(-1) and chilling treatment. The shoot formation of Codonopsis lanceolata was good at the MS medium with 2 mg·L(-1) BA and 2 mg·L(-1) NAA. According to inoculum part, ratio of re-differentiation was higher from the leaf explant compared with the stem explant. For root development, successive subculture with 1 cm shoot of upper part from root was efficient. Roots of Codonopsis lanceolata cultured in vitro system was grown like normal root with length of 3 cm and thickness of 0.5 cm at the 4 successive subcultures.

判別分析에 관한 小考

허순영 건국대학교 1985 商經論叢 Vol.- No.20

韓國語와 英語에 있어서의 Relative clause의 對照 硏究

朴榮順 명지어문학회 1976 明知語文學 Vol.8 No.-

2단 간헐폭기 시스템에서 aeration cycle이 질소 및 인 제거에 미치는 영향

정명선,이준호,서광범,김영관 江原大學校 附設 環境硏究所 2003 環境硏究 Vol.20 No.-

This bench-scale research investigated the aeration cycle(on/off) as the controlling factors for nitrogen and phosphorus removal in a 2-stage, intermittent aeration process. At this experiment, the aeration cycle time(air-on/air-offJ was 30min/30min, 60min/60min, 90min/90min. Organic matter removal was observed more than 90% regardless of the aeration cycle and phosphorus removal was relatively high when the aeration cycle time was 60min/GOmin. On the other hand, For all of the aeration cycle, TN removal was appeared less than 55%. This result was probably due to the limitation of the external substrate for heterotrophic nitrification and aerobic denitrification.

재이식 가능한 32비트 테스크 코어 설계

윤영상,최종화,김용대,한선경,유영갑 충북대학교 컴퓨터정보통신 연구소 2004 컴퓨터정보통신연구 Vol.12 No.1

본 논문에서 제시한 32비트 테스트 코어는 기존의 ARM과 같은 32비트 RISC 코어를 대신하여 JTAG 및 코어브레이커의 회로 테스트를 위하여 고안되었다. 테스트 코어는 디버깅유닛을 바꾸어가며 재이식(retargetable) 가능하게 사용될 수 있다는 장점을 갖는다. 회로 합성 결과 하드웨어는 ARM과 비교하여 33% 정도의 게이트를 가진다. 테스트 코어는 보다 높은 디버깅 성능을 위해 다중로드(multiple load; LDMA)와 다중저장(multiple store; STMA)명령을 추가하였으며 단일로드(single load; LDR) 또는 단일저장(single store; STR)명령들에 비해 64% 정도의 처리시간 단축을 나타내었다. A 32bit RISC type core is designed to replace full processors for testing JTAG and core breaker modules. It is retargetable and can be utilized to test a debugging unit. The design uses about 33% of gates with respect to a fully designed ARM7. It has instructions, multiple load or store(LDMA, STMA), for debugging purposes. Simulation result shows that they reduce the execution time about 64% with respect to processors having a single load or store(LDR, STR) instruction.