中學校 科學科의 實驗實習 敎材의 開發硏究(Ⅲ)

尹世重,趙泰璟,吳濟直,金溫植,金黎相 공주대학교 사범대학 과학교육연구소 1983 과학교육연구 Vol.15 No.1

Teaching materials available for the effective performance of laboratory exp-erimental work in middle school were developed: Physics part ; A measuring apparatus of uniformly accelerated motion and normal force of inclined plane was developed. Chemistry part ; An imploved Eudiometer applicable in measuring reaction mole ratio and usful for a gas generating apparatus was developed. Biology part ; convenient teaching materials observing law of dominance, law of segregation, law of independence, incomplete dominance and dihybrid cross were developed. Earth science part ; A laboratory teaching materials observing formation of wave, current and graded bedding, and identification of sedimentary rocks were developed. Putting the above results of study together, the integrated suggestions were made to the effective development of science education in middle school : 1. The contents of science textbook should be simplified to the direction of the understanding of essential principles and concept. 2. The development and dirtrihbution of various supplementary teaching materials (film, T.P., cassettes film, supplementary textbook, program teaching materials individual learning materials) are required. 3. The experimental laboratory should be equipped with common instruments and equipements. 4. Efforts and time are needed to science teachers, and so remission of sundry duties and arrangement of assistant are hopefully considered. 5. Teacher's research activities should be encouraged by administrative route or relevant institute.

공생진화 알고리듬에서의 공생파트너 선택전략 분석

김재윤,김여근,신태호 한국경영과학회 2000 韓國經營科學會誌 Vol.25 No.4

Symbiotic evolutionary algorithms, also called cooperative coevolutionary algorithms, are stochastic search algorithms that imitate the biological coevolution process through symblotic interactions. In the algorithms, the fitness evaluation of an individual requires first selecting symblotic partners of the individual. Several partner selection strategies are provided. The goal of this study is to analyze how much partnering strategies can influence the performance of the algorithms. With two types of test-bed problems: the NKC model and the binary string covering problem, extensive experiments are carried out to compare the performance of partnering strategies, using the analysis of variance. The experimental results indicate that there does not exist statistically significant difference in their performance.

참돌꽃 근경의 항산화작용

류광열,강원식,김영호,장해동,홍진태,유환수,윤여표 충남대학교 약학대학 의약품개발연구소 1998 藥學論文集 Vol.14 No.-

The purpose of this study was to determine the antioxidative effects of Rhodiola sachalinensis Rhodiola methanol extract was fractionated sequentially with dichloromethane and butanol. Each Rhodiola fraction (water, MeOH, BuOH and CH_2Cl_2 fractions) showed the potent superoxide dismutase (SOD) activity, and had inhibitory effets on peroxide value of linoleic acid (40~57%) and lipid peroxidation (47~70%) in FE^2+/ascorbate system-induced rat liver microsome. Rhodiola methanol extract also recovered carbon tetrachloride-induced decrease in SOD by 42% and catalase activities by 50%, and had inhibitory effects (54%) on carbon tetrachloride-induced lipid peroxidation in rat liver microsome. These results suggest that Rhodiola sachalinensis has the antioxidative effects.

신경성 염증질환에서 지질 신호전달물질인 lactosylceramide의 역할

이윤선, 유재명, 최경미, 이용문, 홍진태, 윤여표, 유환수 충북대학교 약품자원개발연구소 2007 약학논문집 Vol.22 No.-

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Mapping quantitative trait loci for agronomic traits using introgression lines carrying wild rice (Oryza rufipogon) chromosome segments in cultivar background

Sang-Min Yeo,Yeo-Tae Yun,Chong-Tae Chung,Jung-Phil Seo,Hae-Hwang Kim,Sang-Nag Ahn 한국육종학회 2012 한국육종학회 심포지엄 Vol.2012 No.07

The objective of this study were to identify QTLs for agronomic traits using a set of introgression lines carrying wild rice (Oryza rufipogon) segment in cultivated rice (ssp. japonica cv. Hwaseongbyeo). Ninety-six ILs were evaluated for seven agronomic traits, amylose and protein contents. The proportion of the recurrent genome in ILs ranged from 87.8 to 100%, with an average of 96.7%. The mean number of homozygous and heterozygous donor segments were 2 (ranging 0-7) and 1.7 (ranging 0-6), respectively, and the majority of these segments had size less than 10 cM. A total of 22 quantitative trait loci were identified for 9 traits and each QTL explained 7.2% to 56.6% of the phenotypic variance. Some QTLs were clustered in a few chromosomal regions. A first cluster was located near RM527 on chromosome 6 with QTLs for culm length, panicle length, days to heading, 1000-grain weight and protein content. Three ILs with high spikelets per panicle compared to the recurrent parent were selected to detect and fine map the wild segments responsible for this variation. The results will be discussed.

QTL Mapping of Grain Quality Traits Using Introgression Lines Carrying <i>Oryza</i> <i> rufipogon</i> Chromosome Segments in <i>Japonica</i> Rice

Yun, Yeo-Tae,Chung, Chong-Tae,Lee, Young-Ju,Na, Han-Jung,Lee, Jae-Chul,Lee, Sun-Gye,Lee, Kwang-Won,Yoon, Young-Hwan,Kang, Ju-Won,Lee, Hyun-Sook,Lee, Jong-Yeol,Ahn, Sang-Nag Springer US 2016 Rice Vol.9 No.-

<P><B>Background</B></P><P>Improved eating quality is a major breeding target in <I>japonica</I> rice due to market demand. Consequently, quantitative trait loci (QTL) for glossiness of cooked rice and amylose content associated with eating quality have received much research focus because of their importance in rice quality.</P><P><B>Results</B></P><P>In this study, QTL associated with 12 grain quality traits were identified using 96 introgression lines (IL) of rice developed from an interspecific cross between the Korean elite <I>O. sativa japonica</I> cultivar ‘Hwaseong’ and <I>O. rufipogon</I> over 7 years. QTL analyses indicated that QTL <I>qDTH6</I> for heading date, detected on chromosome 6 is associated with variance in grain traits. Most QTLs detected in this study clustered near the <I>qDTH6</I> locus on chromosome 6, suggesting the effect of <I>qDTH6. O. rufipogon</I> alleles negatively affected grain quality traits except for a few QTLs, including <I>qGCR9</I> for glossiness of cooked rice on chromosome 9. To characterize the effect of the <I>O. rufipogon</I> locus harboring <I>qGCR9,</I> four lines with a single but different <I>O. rufipogon</I> segment near <I>qGCR9</I> were compared to Hwaseong. Three lines (<I>O. rufipopgon</I> ILs) having <I>O. rufipogon</I> segment between RM242 and RM245 in common showed higher glossiness of cooked rice than Hwaseong and the other line (Hwaseong IL), indicating that <I>qGCR9</I> is located in the 3.4-Mb region between RM242 and RM245. Higher glossiness of cooked rice conferred by the <I>O. rufipogon</I> allele might be associated with protein content considering that three lines had lower protein content than Hwaseong (<I>P</I> < 0.1). These three <I>O. rufipogon</I> ILs showed higher yield than Hwaseong and Hwaseong IL due to increase in spikelets per panicle and grain weight indicating the linkage of <I>qGCR9</I> and yield component QTLs.</P><P><B>Conclusion</B></P><P>The <I>qGCR9</I> locus is of particular interest because of its independence from other undesirable grain quality traits in <I>O. rufipogon</I>. SSR markers linked to <I>qGCR9</I> can be used to develop high-quality <I>japonica</I> lines and offer a starting point for map-based cloning of genes underlying this trait. To our knowledge, this is the first report to map a beneficial QTL for glossiness of cooked rice from a wild rice, <I>O. rufipogon</I>.</P><P><B>Electronic supplementary material</B></P><P>The online version of this article (doi:10.1186/s12284-016-0135-0) contains supplementary material, which is available to authorized users.</P>

Validation of QTLs associated with spikelets per panicle and grain weight in rice

Yeo, Sang-Min,Yun, Yeo-Tae,Kim, Dong-Min,Chung, Chong-Tae,Ahn, Sang-Nag Cambridge University Press 2014 Plant genetic resources Vol.12 No.1

<P>In this study, a near-isogenic line (BC4F10) CR572 developed by introgressing a chromosomal segment from <I>Oryza rufipogon</I> (accession no. 105491) into the <I>Oryza sativa</I> subsp. <I>japonica</I> cv. Hwaseong was found to exhibit a significant increase in the number of spikelets per panicle (SPP) and grain weight compared with the recurrent parent Hwaseong. Quantitative trait locus (QTL) analysis in F2 generation derived from the cross between CR572 and Hwaseong revealed that two QTLs, <I>qSPP1</I> and <I>qTGW1</I>, were linked to a simple sequence repeat marker, RM283, on chromosome 1. The additive effect of the <I>O. rufipogon</I> allele at <I>qSPP1</I> was 13 SPP, and 21.6% of the phenotypic variance was explained by the segregation of RM283. The <I>qTGW1</I> QTL explained 19.1% of the phenotypic variance for grain weight. Substitution mapping was carried out with five F3 lines derived from F2 plants having informative recombination breakpoints within the target region. Substitution mapping indicated the linkage of <I>qSPP1</I> and <I>qTGW1</I>. The grain yield of CR572 was 18.2 and 15.8% higher than that of Hwaseong at two locations, respectively, mainly due to the increase in 1000-grain weight and SPP. These results are very useful for QTL cluster transfer by molecular marker-assisted selection in rice breeding programmes and for QTL gene cloning by map-based cloning.</P>

Relationship between rice grain quality traits and starch pasting properties using early maturing rice cultivars in Chungnam plain area

Yeo-Tae Yun,Chong-Tae Chung,Jae-Chul Lee,Young-Ju Lee,Han-Jung Na,Kwang-Won Lee,Young-Hwan Yoon 충남대학교 농업과학연구소 2015 농업과학연구 Vol.42 No.3

This study was conducted to know the variation and relationship of rice grain quality and starch pasting properties by transplanting times. Two early maturing rice cultivars which accounted for the most area of early maturing rice cultivar in Chungnam province were used. The experiment was laid out in a split-plot design with 3 replications. The main plot consisted of three transplanting times viz. early (April 25), ordinary (May 25) and late (June 25) with sub-plots containing two cultivars. According to the transplanting times, most of rice grain quality and starch pasting properties showed significant difference and Joami showed higher grain quality than Unkwang in all transplanting times. Especially, rice grain quality was improved when transplanted late, showing high head rice and glossiness of cooked rice due to the lower mean temperature during grain filling stage. Glossiness of cooked rice was positively correlated with head rice ratio, amylose content and setback value, and negatively correlated with chalky rice ratio and protein content. The highest positive and negative correlation were observed between breakdown value and peak viscosity (r = 0.98**), and breakdown and setback (r = -0.94**), respectively. These results provide some information for rice researchers and producers producing cultivars with an improved quality, suggesting that rice quality is highly influenced by temperature at grain filling stage, and transplanting times is crucial in improving rice quality. In addition, starch pasting properties are useful for determining rice quality because rice grain quality and starch pasting properties are dependent on each other.

In Vitro Cellular Uptake Behavior of Polymer Nanoparticles

Tae Yun Yeo,Byung Kook Lee,Yeon Hee Yun,Yong Woo Cho 한국생물공학회 2008 한국생물공학회 학술대회 Vol.2008 No.10

Inhibitory Effects of OD 78 [3-(4-bromo-phenoxy)-4,5-dihydroxybenzoic acid-methyl ester] on the Proliferation and Migration of TNF-α-induced Rat Aortic Smooth Muscle Cells

Yeo-Pyo Yun,Munkhtsetseg Tudev,박은석,Won-Shik Kim,Il-Ho Lim,Mi-Yea Lee,Heesoon Lee,Jae-Kyung Jung,Jin-Tae Hong,유환수,Myung-Koo Lee,Myoung-Yun Pyo,Yeo Pyo Yun,임용 대한약학회 2011 Archives of Pharmacal Research Vol.34 No.7

The proliferation and migration of vascular smooth muscle cells (VSMCs) play important roles in the formation and progression of intimal thickening in early-phase atherosclerosis and in restenosis after vascular injury. Tumor necrosis factor-α (TNF-α) is released from macrophages in atherosclerotic lesions and from neointimal vascular smooth muscle cells after balloon-injury. Obovatol, a major biphenolic component isolated from the Magnolia obovata leaf, is known to have anti-inflammatory and antitumor activities. The goal of this study was to examine the cardioprotective effects of the obovatol derivative OD 78 on the TNF-α-induced proliferation and migration of rat aortic smooth muscle cells (RASMCs). The antiproliferative effects of OD 78 on RASMCs were examined by cell counting and [³H]-thymidine incorporation assays. Treatment of cells with 1-4 μM OD 78 inhibited the proliferation and DNA synthesis of TNF-α-stimulated RASMCs in a concentration-dependent manner, without cytotoxicity. Treatment with OD 78 inhibited TNF-α-mediated p38 phosphorylation, but did not change the activation of extracellular signal-regulated kinase or c-Jun N-terminal kinase. Furthermore, treatment with OD 78 decreased TNF-α-induced levels of cyclin E, cyclin D1, CDK2, proliferating cell nuclear antigen, and phosphorylated retinoblastoma protein, but not the CDK4 expression level. Also, OD 78 inhibits the migration of TNF-α-induced RASMC in transwells. OD 78 treatment strongly decreased matrix metalloproteinase-9 (MMP-9) expression in a dose-dependent manner, but the MMP-2 expression was unchanged. These results show that OD 78 may be developed as a potential antiproliferative agent for the treatment of angioplasty restenosis and atherosclerosis.