톨루엔의 급성흡입이 마우스의 1차 체액성 면역반응에 미치는 영향

표명윤,주은영 숙명여자대학교 약학연구소 1998 약학논문집-숙명여자대학교 Vol.15 No.-

Toluene was inhaled at concentration of 10,000ppm to ICR mice for 10, 20, 30min, and mice were sacrificed on day 2, 4, 6 or 8 following inhalation. Acute toluene inhalation didn't change the body weight gains, thymus and kidney weights. Spleen weight was slightly decreased, but there's no significant difference. Liver weight was significantly decreased on the 6th day after inhalation and recovered on the 8th day after. The number of WBC was significantly decreased on the 4th day and recovery from toluene hazard was appeared on the 8th day. Platelets were markedly and significantly decreased only on the 2nd day in every inhaled group. Red blood cells were increased on the 6th day and recovered on the 8th day after. The number of IgM plaque forming cells in splenocytes and IgM level in antiserum were increased in every inhaled group when toluene( 10,000 ppm, 10, 20, 30 min) was exposed to mice prior to primary immunization. When mice were exposed to toluene after immunization, IgM plaque forming cells and IgM level were increased in 10 min inhaled group and similar to PFC of control in 30 min inhaled group. Therefore the acute toluene inhalation at high dose in mice seems to increase the primary humoral immune response irrespective to the time of SRBC-antigen injection.

새로운 백금착체인 tetrachloro [bis(2-chloroethyl)ethylenediamine-N,N']platinum(IV)과 cisplatin이 마우스의 면역반응에 미치는 영향

표명윤,유경미,최지선,오현정 숙명여자대학교 약학연구소 1993 약학논문집-숙명여자대학교 Vol.9 No.-

Effects of a new platinum complex, tetrachloro-[bis(2-chloroethyl) ethylenediamine-N,N']platinum(IV)[PtCl₄-2(CEen)] and cisplatin on the immune response were investigated. Male ICR mice were treated at the respective LD50(20% or 60% LD50) of cisplatin and PtCl₄-2(CEen). The results were as following : LD50 of PtCl₄-2(CEen) for male ICR mouse was 122.5mg/kg(i.p.) and higher than cisplatin(16.3mg/kg, i.p.). Body-, spleen-, thymus-, liver-weight, number of WBC and RBC were reduced dependently on the dose and day of administeration of cisplatin and PtCl₄-2(CEen). But these toxicities produced by PtC^-^CEen) appeared to be relatively less and to improve faster than by cisplatin. The titers of hemagglutinin and hemolysin to SRBC in mice received cisplatin and PtCl₄-2(CEen) were decreased significantly and independently on the day of antigen injection. Cisplatin and PtCl₄-2(CEen) stimulated significantly contact hypersensitivity to DNFB when administered prior to sensitization(DNFB), but suppressed slightly when administered after sensitization.

톨루엔의 급성 흡입이 마우스의 자발운동량에 미치는 영향

표명윤,주은영 숙명여자대학교 환경과학연구센터 1996 환경과학논문집 Vol.4 No.-

To investigate the effects of acute toluene exposure on the spontaneous locomotor activity. Female ICR mice were exposed for varying periods of time(10, 20, 30min)to 10,000ppm toluene and were tested at intervals during and after exposure. The spontaneous locomotor activity was markedly de­creased at high dose(10,000ppm) toluene inhalation. Rapidity of recovery was dependent upon the time of inhalation.

마우스에서 tolmetin glucuronide와 단백질의 비가역적 결합물의 免疫原性

표명윤 숙명여자대학교 약학연구소 1997 약학논문집-숙명여자대학교 Vol.13 No.-

Immunogenicity of irreversibly bound tolmetin glucuronide-mouse albumin(TG-MA) was investigated in female Balb/c mice. Serum samples were obtained from 4-6 mice of each group immunized by intraperitoneal administration of TG-MA(80, 160/㎕), MA(80/㎕), and PBS on day 0 and day 14. Sera of each group were pooled and diluted (113 or 1:6) with PBS-Tween 20. Wells of microtiter plates were coated with test antigen(TG-MA or MA) at 200/㎕/㎖. Antibody was detected using alkaline phosphatase labeled rabbit anti-mouse IgG(l!500) followed by incubation p- nitrophenyl phosphate. Absorbance was determined at 405nm and antibody binding index(B.I.) for antiserum was calculated. It was found in the animal studies that a direct relationship existed between the dose of TG-MA and the amount of specific IgG anti-TG-MA antibody produced. Specific IgG anti TG-MA antibodies were induced highly and independently on the dose of TG- MA on day 28 after administration.

Dextromethorphan 이 마우스의 비장세포 증식능과 cytokine 생성능에 미치는 영향

표명윤,황유경 숙명여자대학교 약학연구소 2004 약학논문집-숙명여자대학교 Vol.21 No.-

Dextromethorphan hydrobromide (DXM) has been widely used as a nonopioid antitussive drug and is a psychotropic drug since 2003 in our country. This study was performed to investigate the immunotoxicity of DXM by assay of splenocytes proliferation (SP) and induction of cytokine (IFN-γ, IL-4) in vitro. When mouse splenocytes were exposed to various concentration of DXM (0.001, 0.01, 0.1, 1, 10, 100μM) and cultured with T cell mitogen (Con A) or B cell mitogen (LPS), SP to Con A and LPS were significantly decreased at high concentration (100μM) when compared with controls. When splenocytes were cultured with DXM in the presence of Con A, levels of IFN-γ in culture supernatant were slightly decreased at low concentration, and significantly decreased at high concentration (100μM). DXM also suppressed IL-4 secretion significantly at 100μM, however, increased the production of IL-4 at lower concentration when compared with control group. This study provides the substantial evidence on DXM-induced alternation in cell-mediated and humoral immunity.

Dextromethorphan이 마우스의 자발운동량에 미치는 영향

표명윤,유경미 숙명여자대학교 약학연구소 1999 약학논문집-숙명여자대학교 Vol.16 No.-

The effect of dextromethorphan(Dex) on the spontaneous locomotor activity in mice was investigated. After Dex was intraperitoneally adminstered with the dose of 20, 60 or 80 mg/kg/day once a day for 1 day, 4 days or 7 days in female ICR mice. The spontaneous locomotor activity such as distance traveled(DT) was observed for 3.5 hrs at 15 min intervals. The spontaneous locomotor activity was markedly increased and showed to be biphasic in a dose dependent manner.

2- [4-cyanophenyl)amino)]-3-chIoro-1,4-naph thalened ione (NQ-Y15)가 마우스의 면역병리 및 체액성 면역에 미치는 영향

표명윤 숙명여자대학교 약학연구소 2002 약학논문집-숙명여자대학교 Vol.18 No.-

In order to investigate the effects of 2-[(4-cyanophenyl)amino]-3-chloro-l,4-naphthalenedione (NQ-Y15) on immune system in mice, we examined the immunopathological parameters and splenic IgM plaque forming cells (PFC) in this study. The immunopathological parameters (weights of body and organs, hematological parameters) were examined on day 1, 2,4 and 6 following single oral administration of NQ-Y15 (125, 250, 500 mg/kg) to female ICR mice. The weight of body was not changed. However, the weights of organs were slighty increased, and among hematological parameters WBC and platelet (PLT) were slightly changed in a dose- and test day-dependent manner when compared with control group. When NQ-Y15 was administered before immunization with SRBCantigen, PFC was significantly and dose-independently enhanced, but PFC was slightly decreased only at a high dose of NQ-Y15 when administered after antigenic challenge. These results indicate that the effects of NQ-Y15 on primary humoral immune response may be dependent on the timing of its administration relative to the initial antigenic challenge.

Tetrachloro-[bis(2-chloroethyl)ethylene-diamine-N,N']platinum(Ⅳ)와 cisplatin이 마우스의 자연 살해세포 활서능에 미치는 영향

표명윤,고숙경 숙명여자대학교 약학연구소 1994 약학논문집-숙명여자대학교 Vol.10 No.-

Effects of tetrachloro-[bis(2-chloroethyl) ethylene-diamine-N,N'] platinum(IV), PtCl₄(2-CEen) and cisplatin on natural killer(NK) cell activity of spleen cells were investigated. To compare NK cell activity by PtCl₄(2-CEen) with that by cisplatin, male ICR mice received a single i.p. injection at the respective LD_(50) of cisplatin and PtCl₄(2-CEen), namely 20% LD_(50)(3.3mg/kg and 24.5mg/kg, respectively) or 40% LD_(50)(6.6mg/kg and 49mg/kg, respectively). Both cisplatin and PtCl₄(2-CEen) showed significant and dose-dependent enhancement of NK cytotoxicity against YAC-1 cells. PtCl₄(2-CEen) enhanced NK cell activity slightly more at low dose than cisplatin, but at high dose cisplatin showed slightly higher cytotoxicity.

Carboxyethylgermanium sesquioxide(Ge-132)가 항암제의 세포독성에 미치는 영향

표명윤,민순홍,곽영희 숙명여자대학교 약학연구소 1998 약학논문집-숙명여자대학교 Vol.14 No.-

The cytotoxicity of carboxyethylgermanium sesquioxide(Ge-132) and anticancer drugs (cisplatin, mitomycin C, 5-fluorouracil) against tumor cell lines(L1210, SK-HepG-1) and mouse splenocytes was examined by MTT assay. Ge-132(8.5 x 10²㎕/ml, 1.7 X 10³㎕/ml, 3.4 x 10³㎕/ml) showed dose-dependent cytotoxicity against the tumor cell lines, on the contrary, increased cell proliferation against the normal mouse splenocytes. Combination of Ge- 132 with cisplatin(1.5㎕/ml, 15㎕/ml), mitomycin C(0.5㎕/ml, 1.0㎕/ml) or 5-fluorouracil (0.31㎕/ml, 1.25㎕/ml) showed additional cytotoxicity against L1210 and SK-HepG-1 cell lines, however, reduced clearly the anticancer drugs-induced cytotoxicity against the normal mouse splenocytes.

Toluene diisocyanate(TDI) 및 Diphenylmethane diisocyanate(MDI)의 變異原性과 細胞毒性과의 相關性 硏究

표명윤,양미희 숙명여자대학교 약학연구소 1995 약학논문집-숙명여자대학교 Vol.11 No.-

TDI(Toluene diisocyante) and MDI(Diphenylmethane diisocyanate) used as hardners in the production of polyurethane, showed following cytotoxicity and mutagenic activities. 1. Using TA98 without metabolic activation(S_9), TDI at the does, 78-625㎍/plate, completely inhibited TA98 growth. However TDI at the higher does than that, stimulated bacterial growth. With metabolic activation(+S_9), simillar tendency was observed but the magnitude of cytotooxicities of _S_9 was weaker than those of -S_9, Cytotoxicity pattern of MDI was similar to that of TDI, but its potency is higher than TDI's. 2. TDI and MDI appeared to be mutagenic to TA98 only after metabolic activation and this was more apparent when their cytotoxicities were diminished. Although results are not conclusive, they also showed similar tendency in the case of S_9 free condition. 3. TDI and MDI showed significant mutagenic action on TA100 only after the presence of S_9 mix, without any effect on TA100 growth. In concusion, considering the facts that TDI and MDI have strong cytotoxicites and there was increasing tendency in the mutagenic activities accompanied decrease of cytotoxicities, it could not be ruled out that TDI and MDI are not only secondary mutagens but also primary mutagens.