Cardioprotective effect of KR-33889, a novel PARP inhibitor, against oxidative stress-induced apoptosis in H9c2 cells and isolated rat hearts

박은석,강도현,강준철,장용창,이민주,정훈종,이규양,김대은,김보경,신화섭 대한약학회 2017 Archives of Pharmacal Research Vol.40 No.5

Oxidative stress plays a critical role in cardiacinjury during ischemia/reperfusion (I/R). Despite a potentcardioprotective activity of KR-33889, a novel poly (ADPribose)polymerase inhibitor, its underlying mechanismremains unresolved. This study was designed to investigatethe protective effects of KR-33889 against oxidative stressinducedapoptosis in rat cardiomyocytes H9c2 cells andisolated rat hearts. H2O2 caused severe injury to H9c2 cells,mainly due to apoptosis, as revealed by TUNEL assay. However, KR-33889 pretreatment significantly attenuatedH2O2-induced apoptosis of H9c2 cells, which wasaccompanied by decrease in expression of both cleavedcaspase-3 and Bax and increase in Bcl-2 expression and theratio of Bcl-2/Bax. KR-33889 also significantly enhancedthe expression of anti-oxidant enzymes including hemeoxygenase-1, Cu/Zn-superoxide dismutase (SOD), Mn-SOD, and catalase, thereby inhibiting production of intracellularROS. Furthermore, KR-33889 reversed H2O2-induceddecrease in phosphorylation of Akt, GSK-3b, ERK1/2, p38 MAPK, and SAPK/JNK during most H2O2 exposuretime. In globally ischemic rat hearts, KR-33889 inhibitedboth I/R-induced decrease in cardiac contractility andapoptosis by increasing Bcl-2, decreasing both cleavedcaspase-3 and Bax expression, and enhancing expression ofanti-oxidant enzymes. Taken together, these results suggestthat KR-33889 may have therapeutic potential to preventI/R-induced heart injury in ischemic heart diseases mainlyby reducing oxidative stress-mediated myocardialapoptosis.

환경, 에너지 그리고 순환 : 구두 ; 폐기물 열처리 및 배기가스 처리 : 청정오일 생산을 위한 하수슬러지의 열분해

박은석,강보성,김주식 한국폐기물자원순환학회 2007 2007 환경공동학술대회 Vol.2007 No.1

고등학교 태권도 선수들의 무산소성 파워

박은석 한국스포츠리서치 2006 한국 스포츠 리서치 Vol.17 No.3

원보 ; 생분해성 고분자로의 물의 유입속도 측정

박은석,지상철 ( Eun Seok Park,Sang Cheol Chi ) 한국약제학회 1995 Journal of Pharmaceutical Investigation Vol.25 No.3

A new method to study water uptake into biodegradable polymer was developed. Polymer was gently mixed with the tritiated buffer and the radioactivity level of the polymer due to water uptake was measured by liquid scintillation counting. The rate of water uptake was estimated from the plot of the amount of water in the device as a function of time. The technique used in this study is particularly useful for estimating water uptake of biodegradable polymers which were difficult to study by other techniques such as weight gain used for hydrogel.

타이완 지역 오스트로네시아어 사동 연구

박은석 중앙대학교 외국학연구소 2023 외국학연구 Vol.- No.63

This paper is a study on the causative of Austronesian languages in Taiwan. These languages have a syntactic causative, morphological causative, lexical causative, and continuums between syntactic and morphological causative. Syntactic causative of These languages belongs to complex sentence with a pivot structure. On the other hand, lexical causative and morphological causative belong to simple sentence structure. Some of these languages ​​have structures that combine syntactic causative and morphological causative. Tsou language has a causative continuum between syntactic causative and morphological causative formed using the particle 'poa'. Morphological causative is the most commonly used causative structure of Austronesian languages in Taiwan, and is constructed by using a causative affix or a focal affix as a causative affix. In some of these languages, depending on whether a causative affix is ​​added to a dynamic verb or a static verb (including adjectives), the type of causative affix may change or there may be restrictions on adding affixes. Also, about half of these languages ​​are characterized by being able to add a causative affix to a noun. Yami language can even add a causative affix to an onomatopoeia. In the Austronesian languages ​​of Taiwan, there is also a lexical causative structure that does not change the form between the original verb and the causative verb. In Saisiyat language, it was found that when the prefix 'in-' is added to a lexical causative verb, it changes to an intransitive verb. And as causer in the causative sentences of Austronesian languages in Taiwan, human nouns(or personal pronouns), animal nouns, natural object nouns, object nouns, and abstract nouns appear. In the Sakizaya language, constraints was found that required the appearance of inanimate noun phrases. Also, as causee in the causative sentences of Austronesian languages in Taiwan, human nouns(or personal pronouns), animal nouns, natural object nouns, object nouns, and abstract nouns appear. In Sakizaya and Amis languages, the prefix 'pi-' have functions to increase the causee's control over the causative situation. In terms of directness and indirectness of causative, Austronesian languages ​​in Taiwan generally show more direct meanings when the degree of fusion between the predicate of the cause event and the predicate of the effect event increases. In addition, it was found that the prefix 'pi-' in Amis language can contribute to increasing the indirectness of causative.

중국어 용언성 성분의 시간참조 및 그 통사 효과

박은석 중국어문논역학회 2020 中國語文論譯叢刊 Vol.0 No.46

Guo rui said “Predicative constituents in modern Chinese carry two types of time references. One is internal time reference which is the followed event of the main event in a sentence. The other is external time reference which is a certain point on the natural time axel of the world. External time reference locates the event a sentence expresses in the realistic world, while the internal time reference shows the temporal relations of several events in a sentence. It has no connection with the timing of the realistic world. The semantic function of le, zhe and ɡuo can be explained from the perspective of time reference. The reason that sentences with VPs carrying le1, zhe and ɡuo1 are not accepted as full sentences is the absence of external time reference. Only constituents indicating time reference such as followed VPs, numeral phrases, le2or ne are added that these sentences are taken as full sentences. Narrative sentences with le1 in modern Chinese novels are founded as full sentences. This kind of usage of le1 infers that it is in the process of developing into a past tense marker.” 현대중국어 용언성 성분의 시간참조에는 두 가지 종류가 있다. (1) 내부참조: 문장 중에서 후속동작이 발생한 시간을 참조로 삼는 것이다. (2) 외부참조: 외부세계의 자연시간 과정 중의 어느 한 시각을 참조로 삼는 것이다. 외부참조는 문장이 나타내는 상황이 현실 세계 안에서 위치를 고정할 수 있도록 한다. 반면 내부참조는 문장 내부의 여러 상황 간의 시간 관계를 나타내주기만 할 뿐 현실세계의 시간과는 무관하다. 시간 참조의 각도에서 ‘了, 着 , 過’의 의미 기능을 설명할 수 있다. ‘了₁’, ‘着’, ‘過₁’를 대동한 VP가 문장을 종결할 수 없는 것은 외부참조를 결여하고 있기 때문이다. ‘了₁’, ‘着’, ‘過₁’를 대동한 VP가 문장을 종결하려면 시간참조를 제공할 수 있는 성분, 예를 들면 후속 VP, 수량구, 혹은 외부시간참조를 부가하는 ‘了₂’, ‘呢’ 등이 반드시 부가되어야 한다. 현대중국어 소설의 서술문 중에는 ‘了₁’으로 문장을 종결하는 경우가 있는데, 이러한 용법은 ‘了₁’이 과거시제표지로 변해가고 있음을 나타내는 것이다.

중국어 교육에 있어서 즉흥극의 실례, 효과, 필요성

박은석 한국중국언어학회 2016 중국언어연구 Vol.0 No.63

This paper analyzes the teaching effect of "improvised drama" in the teaching of university Chinese in the case of the "Intermediate Chinese language 2" of A University, and emphasizes the importance of using this kind of teaching method. Communicative language teaching scholars generally believe that the "improvised drama" is a kind of the extreme expansion of students' creative teaching methods in university Chinese teaching, but has not been widely used. This paper analyzes the main factors that cause the Chinese teacher to avoid using the "improvised drama", and puts forward the method to solve this problem. From the students on the evaluation of the "Intermediate Chinese language 2", this course, "Improvisational drama" can stimulate the interest of students in Chinese language and courses, for students to create a relaxed and pleasant learning environment, promote the interaction between students and also can effectively guide the students to review the learned content, make the students creativity, application ability and the strain capacity is improved, so that students want to learn Chinese. "Improvisational drama" is a group activity, but compared with other group activities, is undoubtedly a kind of the most effective means to improve the teaching effect. Therefore, Korean university education in the classroom should be more active use of "Improvisational drama" this teaching method. 本文以A大学教养课程<中级中国语2>为例,分析了大学汉语教学时,“即兴剧”这种授课方式的教学效果,并强调了使用这种教学方式的重要性。交际语言教学学界一般认为“即兴剧”是一种能把学生创造性极度扩大化的教学方式,但是在大学汉语教学中并没有得到普遍使用。本文分析了造成汉语教师回避使用“即兴剧”这种方式的主要因素,并提出了解决这一问题的方法。 从学生们对《中级中国语2》这门课的教学评价来看,“即兴剧”可以激发学生们对汉语和课程的兴趣,为学生创造一个轻松、愉快的学习环境,促进学生之间的互动,还可以有效引导学生复习学过的内容,使学生的创造力、应用能力和应变能力得到提高,让学生想继续学习汉语。 “即兴剧”是一种小组活动,但是和其他的小组活动相比,无疑是一种能够提高教学效果的最有效的手段。因此韩国的大学教养汉语课堂上应更积极使用“即兴剧”这种授课方式。

현대 표준 중국어의 술보형 사동 연속체 ― 허사격리술보형 사동, 목적어격리술보형 사동, 비분리술보형 사동 연구

박은석 중국어문학회 2017 中國語文學誌 Vol.0 No.59

This study is about Mandarin Chinese resultative causative continuum, expletive separating resultative causatives, object separating resultative causatives, non-separable resultative causatives. These three resultative causatives are the continuum between analytic causatives and morphological causatives. This study investigates and compares the formal features and semantic features of these three resultative causatives. The features are causer type features, causer hierachy of animacy features, causer`s volition features, causee type features, causee`s control of result event features, causative predicate type features, implicativity features, and sentence type features.

Benchtop NMR을 이용한 화학물질 구조 및 정량분석

박은석 한국분석과학회 2025 한국분석과학회 학술대회 Vol.2025 No.11

태권도장 태권도수련생 학부모의 COVID19 위험지각이 비대면수업 서비스사용성에 미치는 영향

박은석,김정모 대한체육학회 2022 대한스포츠융합학회지 (jcses) Vol.20 No.2

The purpose of this study is to analyze the effect of the COVID-19 risk perception, recognized by those parents of Taekwondo practitioners, at a Taekwondo Gym, regarding the usability of untact classes. In order to achieve the purpose of the study, empirical data were collected from 320 people through a survey method, and data from 304 people was used for statistical analysis at first. For statistical analysis, SPSS 28.0 for Windows statistical package program was used, and for hypothesis testing, multiple regression analysis was used for statistical verification. First, the risk perception of COVID-19 was negative (-) on the ‘economic (β=-.357)’ on the ‘social (β=-.262)’ and ‘physical (β=-.125)’ factors in ‘learning’, a sub-factor of ‘service usability’ (p<.01, p<.001). Second, the risk perception of COVID-19 showed a negative (-) effect on the ‘physical (β=-.391)’ on the ‘economic (β=-.431)’ and ‘social (β=-.123)’ factors in ‘convenience of use’, a sub-factor of ‘service usability’(p<.05, p<.001). Third, the perception of COVID-19 risk showed a negative (-) effect on the ‘physical (β=-.226)’ on the ‘social (β=-.294)’ on the ‘environmental (β=-.286)’, and ‘economic (β=-.236)’ in ‘usefulness’, a sub-factor of ‘service usability’(p<.001). In conclusion, we obtained the research result that the perception of COVID-19 risk, perceived by the parents of Taekwondo practitioners, at the Taekwondo Gym, partially affects the usability of untact classes.