Rhein lysinate decreases inflammation and adipose infiltration in KK/HlJ diabetic mice with non-alcoholic fatty liver disease

Jie Wei,Yong-Zhan Zhen,Ju Cui,Fa-Lin He,Tao Shen,Gang Hu,Xiao-Hong Ren,Yajun Lin 대한약학회 2016 Archives of Pharmacal Research Vol.39 No.7

The objective of this study was to investigate the protective effects of rhein lysinate (RHL) on the liver. Mice were divided into four groups: C57BL/J control, the KK/HlJ diabetic model, and 25 and 50 mg/kg/day RHLtreated KK/HlJ groups. The KK/HlJ diabetic mouse model was made by injecting STZ and feeding mice diabetic food. At 16 weeks, mice were sacrificed and their livers were harvested. The results indicated that compared with the C57BL/J control group, the body weights, liver weights and liver weight-to-body weight ratio were increased in KK/HlJ diabetic mice; however, these values were decreased following treatment with RHL. Compared with the C57BL/J control, KK/HlJ diabetic mice had a significantly lower level of SOD and GSH-px in their livers, but had a significantly higher level of MDA. However, these effects were ameliorated by RHL. Hepatic adipose infiltration was observed in KK/HlJ mice, but not in C57BL/J mice. RHL decreased the incidence of hepatic adipose infiltration and significantly decreased the expression of TNF-a, IL-6, NF-jB, SREBP-1c, and Fas, as well as the phosphorylation of NF-jB in the liver. In conclusion, RHL can improve hepatic function by decreasing hepatic adipose infiltration and the expression of inflammatory factors.

Rapid and Efficient Detection of 16SrI Group Areca Palm Yellow Leaf Phytoplasma in China by Loop-Mediated Isothermal Amplification

Shao-shuai Yu,Hai-yan Che,Sheng-jie Wang,Cai-li Lin,Ming-xing Lin,Wei-wei Song,Qing-hua Tang,Wei Yan,Wei-quan Qin 한국식물병리학회 2020 Plant Pathology Journal Vol.36 No.5

Areca palm yellow leaf (AYL) disease caused by the 16SrI group phytoplasma is a serious threat to the development of the Areca palm industry in China. The 16S rRNA gene sequence was utilized to establish a rapid and efficient detection system efficient for the 16SrI-B subgroup AYL phytoplasma in China by loopmediated isothermal amplification (LAMP). The results showed that two sets of LAMP detection primers, 16SrDNA-2 and 16SrDNA-3, were efficient for 16SrIB subgroup AYL phytoplasma in China, with positive results appearing under reaction conditions of 64oC for 40 min. The lowest detection limit for the two LAMP detection assays was the same at 200 ag/μl, namely approximately 53 copies/μl of the target fragments. Phytoplasma was detected in all AYL disease samples from Baoting, Tunchang, and Wanning counties in Hainan province using the two sets of LAMP primers 16SrDNA-2 and 16SrDNA-3, whereas no phytoplasma was detected in the negative control. The LAMP method established in this study with comparatively high sensitivity and stability, provides reliable results that could be visually detected, making it suitable for application and research in rapid diagnosis of AYL disease, detection of seedlings with the pathogen and breeding of diseaseresistant Areca palm varieties.

Molecular Cloning, Characterization and Expression of a Novel Trehalose-6-phosphate Synthase Homologue from Ginkgo biloba

Wei Sheng Wu,Yong Zhen Pang,Guo An Shen,Jie Lu,Juan Lin,Jin Wang,Xiao Fen Sun,Ke Xuan Tang 한국생활환경학회 2006 BMB Reports Vol.39 No.2

Development of Integrated Multi-Station System to Precisely Detect and Mitigate Surface Damage on Fused Silica Optics

Lin-Jie Zhao,Jian Cheng,Ming-Jun Chen,Xiao-Dong Yuan,Wei Liao,Hao Yang,Qi Liu,Hai-Jun Wang 한국정밀공학회 2021 International Journal of Precision Engineering and Vol.22 No.1

In high power laser facility, irreversible damage on fused silica optics, induced by laser irradiation or processing, seriously affects the service life of optics. Therefore, the work of inhibiting damage growth has been carried out in various countries. In our work, an integrated multi-station system is designed to detect and mitigate surface damage on fused silica. The process of processing fused silica optics include UV laser conditioning, surface damage detection and surface damage mitigation with CO2 laser. UV laser conditioning pre-initiates surface damage on fused silica optics with the laser flux less than Laser-Induced Damage Threshold (LIDT). Images of surface damage acquired from camera are processed by improved global threshold segmentation algorithm to extract damage information. Finally, CO2 laser is applied to process the damage with specific morphology to enhance the laser damage resistance. This integrated multi-station system saves the repeated optics installation time between the workstations with the positioning accuracy of 20 μm. Furthermore, the damage with diameter of 10 μm is mitigated to prolong service life of processed fused silica optics. The efficient and accurate integrated multi-station system is of great significance for off -line detecting and mitigating surface damage of fused silica optics in high power laser facility.

Systemic Inflammatory Biomarkers, Especially Fibrinogen to Albumin Ratio, Predict Prognosis in Patients with Pancreatic Cancer

Lin Fang,Fei-Hu Yan,Chao Liu,Jing Chen,Dan Wang,Chun-Hui Zhang,Chang-Jie Lou,Jie Lian,Yang Yao,Bo-Jun Wang,Rui-Yang Li,Shu-Ling Han,Yi-Bing Bai,Jia-Ni Yang,Zhi-Wei Li,Yan-Qiao Zhang 대한암학회 2021 Cancer Research and Treatment Vol.53 No.1

Purpose Systemic inflammatory response is a critical factor that promotes the initiation and metastasis of malignancies including pancreatic cancer (PC). This study was designed to determine and compare the prognostic value of neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte ratio (PLR), monocyte-to-lymphocyte ratio (MLR), and fibrinogen-to-albumin ratio (FAR) in resectable PC and locally advanced or metastatic PC. Materials and Methods Three hundred fifty-three patients with resectable PC and 807 patients with locally advan-ced or metastatic PC were recruited in this study. These patients were classified into a training set (n=758) and a validation set (n=402). Kaplan-Meier survival plots and Cox proportional hazards regression models were used to analyze prognosis. Results Overall survival (OS) was significantly better for patients with resectable PC with low preoperative PLR (p=0.048) and MLR (p=0.027). Low FAR, MLR, NLR (p < 0.001), and PLR (p=0.003) were significantly associated with decreased risk of death for locally advanced or metastatic PC patients. FAR (hazard ratio [HR], 1.522; 95% confidential interval [CI], 1.261 to 1.837; p < 0.001) and MLR (HR, 1.248; 95% CI, 1.017 to 1.532; p=0.034) were independent prognostic factors for locally advanced or metastatic PC. Conclusion The prognostic roles of FAR, MLR, NLR, and PLR in resectable PC and locally advanced or metastatic PC were different. FAR showed the most prognostic power in locally advanced or metastatic PC. Low FAR was positively correlated with OS in locally advanced or metastatic PC, which could be used to predict the prognosis.

An Unstructured Kinetic Model for the Improvement of Triterpenes Production by Ganoderma lucidum G0119 Based on Nitrogen Source Effect

Jie Feng,Jing-Song Zhang,Wei Jia,Yan Yang,Fang Liu,Chi Chung Lin 한국생물공학회 2014 Biotechnology and Bioprocess Engineering Vol.19 No.4

The kinetics of cell growth and triterpenesproduction for liquid submerged fermentation of themedicinal mushroom Ganoderma lucidum were investigated. A kinetic model was developed based on the Logistic andLuedeking-Piret equations for cell growth, substrateconsumption and triterpene formation. The kinetic parametersof the model were optimized by specifically designedRunge-Kutta genetic algorithms. The mathematical modelsimulated the experimental data well and was capable ofexplaining the behavior of triterpenes production. Thepredictions of the kinetics from this model are very goodboth for normal fermentation kinetics under nitrogenlimitation as well as for predictions of transitions to sluggishfermentations. The resulting model is very useful forscaling up liquid submerged fermentation of the mushroomG. lucidum and its application to the industrial productionof triterpene.

Estimation of the net energy requirement for maintenance in broilers

Wei Liu,Chang Hua Lin,Zheng Ke Wu,Guo Hua Liu,Hai Jie Yan,Hua Ming Yang,Hui Yi Cai 아세아·태평양축산학회 2017 Animal Bioscience Vol.30 No.6

Objective: The net energy requirement for the maintenance (NEm) of broilers was determined using regression models by the indirect calorimetry method (ICM) or the comparative slaughter method (CSM). Methods: A 2×4 factorial arrangement of treatments including the evaluation method (ICM or CSM) and feed intake (25%, 50%, 75%, or 100% of ad libitum recommended) was employed in this experiment. In the ICM, 96 male Arbor Acres (AA) birds aged d 15 were used with 4 birds per replicate and 6 replicates in each treatment. In the CSM, 116 male AA birds aged d 15 were used. Among these 116 birds, 20 were selected as for initial data and 96 were assigned to 4 treatments with 6 replicate cages and 4 birds each. The linear regression between retained energy (RE) and metabolizable energy intake (MEI) or the logarithmic regression between heat production (HP) and MEI were used to calculate the metabolizable or net energy requirement for maintenance (MEm) or NEm, respectively. Results: The evaluation method did not detect any differences in the metabolizable energy (ME), net energy (NE), and NE:ME of diet, and in the MEI, HP, and RE of broilers. The MEI, HP, and RE of broilers decreased (p<0.01) as the feed intake decreased. No evaluation method× feed intake interaction was observed on these parameters. The MEm and NEm estimated from the linear relationship were 594 and 386 kJ/kg of body weight (BW)0.75/d in the ICM, and 618 and 404 kJ/kg of BW0.75/d in the CSM, respectively. The MEm and NEm estimated by logarithmic regression were 607 and 448 kJ/kg of BW0.75/d in the ICM, and were 619 and 462 kJ/kg of BW0.75/d in the CSM, respectively. Conclusion: The NEm values obtained in this study provide references for estimating the NE values of broiler diets.

Isoprenaline Induces Periostin Expression in Gastric Cancer

Lin Chen,Guo-Xiao Liu,Hong-Qing Xi,Xiao-Yan Sun,Zhi-Jun Geng,Shao-Wei Yang,Yan-Jie Lu,Bo Wei 연세대학교의과대학 2016 Yonsei medical journal Vol.57 No.3

Purpose: Periostin mediates critical steps in gastric cancer and is involved in various signaling pathways. However, the roles of periostin in promoting gastric cancer metastasis are not clear. The aim of this study was to investigate the relevance between periostinexpression and gastric cancer progression and the role of stress-related hormones in the regulation of cancer development and progression. Materials and Methods: Normal, cancerous and metastatic gastric tissues were collected from patients diagnosed with advanced gastric cancer. The in vivo expression of periostin was evaluated by in situ hybridization and immunofluorescent staining. Meanwhile,human gastric adenocarcinoma cell lines MKN-45 and BGC-803 were used to detect the in vitro expression of periostin by using quantitative real-time polymerase chain reaction (PCR) and western blotting. Results: Periostin is expressed in the stroma of the primary gastric tumors and metastases, but not in normal gastric tissue. In addition,we observed that periostin is located mainly in pericryptal fibroblasts, but not in the tumor cells, and strongly correlated to the expression of α-smooth muscle actin (SMA). Furthermore, the distribution patterns of periostin were broader as the clinical staging of tumors progressed. We also identified a role of stress-related signaling in promoting cancer development and progression,and found that isoprenaline upregulated expression levels of periostin in gastric cancer cells. Conclusion: These findings suggest that the distribution pattern of periostin was broader as the clinical staging of the tumor progressedand found that isoprenaline upregulated expression levels of periostin in gastric cancer cells.

Analysis of the Relationship between Breaking Stress and Whiteness in a New Hair Bleaching Method

Wei Wang,Qing-hui Mao,Yu Zhang,Jian-gang Qu,Zhi-jie Liang,Jia Yu,Lin-juan Zheng,Li Zhang 한국섬유공학회 2020 Fibers and polymers Vol.21 No.12

In order to investigate the relationship between breaking stress and whiteness in a bleaching treatment withlaccase, hair samples from three biogeographically distinct populations were characterized by various chemical and physicaltechniques. Factors affecting whiteness and breaking stress were investigated by attenuated total reflection infraredspectroscopy, atomic absorption spectroscopy, X-ray diffraction and scanning electron microscopy. The results reveal thatwhiteness and breaking stress after bleaching are primarily influenced by the diversity of concentration of metal ions andfunctional groups in the hair. The hair samples with lower content of metal ions obtain better whiteness and higher stressretention. The presence of OH functional group increases the final whiteness of the hair sample, while lack of that not onlydecreases the whiteness but also leads to more stress loss. In addition, the presence of the C=C functional group alsodecreases the whiteness.

Mapping out the surgical anatomy of the lingual nerve: a systematic review and meta-analysis

Lin Sheena Xin Yi,Sim Paul Ruiqi,Lai Wei Ming Clement,Lu Jacinta Xiaotong,Chew Jacob Ren Jie,Wong Raymond Chung Wen 대한구강악안면외과학회 2023 대한구강악안면외과학회지 Vol.49 No.4

Objectives: Understanding the lingual nerve’s precise location is crucial to prevent iatrogenic injury. This systematic review seeks to determine the lingual nerve’s most probable topographical location in the posterior mandible. Materials and Methods: Two electronic databases were searched, identifying studies reporting the lingual nerve’s position in the posterior mandible. Anatomical data in the vertical and horizontal dimensions at the retromolar and molar regions were collected for meta-analyses. Results: Of the 2,700 unique records identified, 18 studies were included in this review. In the vertical plane, 8.8% (95% confidence interval [CI], 1.0%-21.7%) and 6.3% (95% CI, 1.9%-12.5%) of the lingual nerves coursed above the alveolar crest at the retromolar and third molar regions. The mean vertical distance between the nerve and the alveolar crest ranged from 12.10 to 4.32 mm at the first to third molar regions. In the horizontal plane, 19.9% (95% CI, 0.0%-62.7%) and 35.2% (95% CI, 13.0%-61.1%) of the lingual nerves were in contact with the lingual plate at the retromolar and third molar regions. Conclusion: This systematic review mapped out the anatomical location of the lingual nerve in the posterior mandible, highlighting regions that warrant additional caution during surgeries to avoid iatrogenic lingual nerve injuries.