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Licheng Liu,Xiaoxiang Li,Sanxiong Liu,Jun Min,Wenqiang Liu,Xiaowu Pan,Baohua Fang,Min Hu,Zhongqi Liu,Yongchao Li,Haiqing Zhang 한국유전학회 2021 Genes & Genomics Vol.43 No.4
Background Rice (Oryza sativa L.) is an important crop and a staple food for half of the population around the world. The recent water and labor shortages are encouraging farmers to shift from traditional transplanting to direct-seeding. However, poor germination and slow elongation of the coleoptile constrains large-scale application of direct-seeding. Objective Thisstudy was aimed to investigate the genetic basis of the anaerobic germination(AG) potential using a set of Oryza nivara (O. nivara) introgressionlines (ILs). Methods Inthis study, a total of 131 ILs were developed by introducing O. nivara chromosomesegments into the elite indica rice variety 93-11 through advanced backcrossingand repeated selfng. A high-density genetic map has been previouslyconstructed with 1,070 bin-markers. The seeds of ILs were germinated and usedto measure coleoptile length under normal and anaerobic conditions. QTLsassociated with AG potential were determined in rice. Results Basedon the high-density genetic map of the IL population, two QTLs, qAGP1 and qAGP3 associated with AG tolerance were characterized and locatedon chromosomes 1 and 3, respectively. Each QTL explained 15% of the phenotypic variance.Specifcally, the O. nivara-derived chromosomesegments of the two QTLs were positively tolerance to anaerobic condition byincreasing coleoptile length. In a further analysis of public transcriptomedata, a total of 26 and 36 genes within qAGP1 and qAGP3 were transcriptionallyinduced by anaerobic stress, respectively. Conclusions Utilizationof O. nivara-derived alleles at qAGP1 and qAGP3 can potentially enhance tolerance to anaerobic stress at thegermination stage in rice, thereby accelerating breeding of rice varieties tobe more adaptative for direct-seeding.
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가격복잡성, 소비자혼란 및 쇼핑피로감에 기반한 온라인 호텔 예약 지연
류팡팡(Fang-Fang Liu),설훈구(Hoon-Ku Sul) 한국관광학회 2024 관광학연구 Vol.48 No.2
중국에서 지속적으로 증가하고 있는 모바일 호텔예약 앱들은 사용자들에게 폭넓은 숙박 옵션과 다양한 가격대를 제공하나, 가격의 복잡성과 정보 과다로 인해 사용자의 혼란을 야기하고 결정을 미루게 만들고 있다. 이에 대응하여, 모바일 예약 서비스 제공자들은 소비자의 혼란을 완화하고 예약 절차를 단순화하여 소비자 만족도를 높이고 장기적인 소비자 유지를 도모할 수 있는 전략을 개발하고 실행할 필요성이 대두되고 있다. 이러한 배경에서 이 연구는 중국 모바일 호텔예약 앱 서비스 환경에서 가격복잡성, 소비자혼란, 쇼핑피로감 및 구매지연 사이의 영향관계를 살펴보았으며, 특히 이러한 영향 관계에서 쇼핑피로감의 매개효과, 예약경험의 조절효과와 조절된 매개효과에 대해 실증적 검정을 수행하였다. 연구를 위해 중국 모바일 호텔예약 앱을 이용하여 호텔을 예약한 경험이 있는 사용자를 대상으로 온라인 설문 조사를 진행하였으며, 305명의 유효한 표본을 최중 분석에 활용하였다. 연구 결과, 첫째, 중국 모바일 호텔예약 앱 서비스의 가격복잡성과 소비자혼란은 모두 쇼핑피로감과 구매지연에 유의한 정적 영향을 미친 것으로 나타났다. 둘째, 소비자의 쇼핑피로감이 구매지연에 정적 영향을 미치며, 가격복잡성, 소비자혼란, 및 구매지연 사이의 관계에서 쇼핑피로감이 부분적 매개효과를 가지는 것으로 확인되었다. 마지막으로, 소비자혼란과 구매지연 간의 관계에서 예약경험이 조절역할과 조절된 매개역할을 수행한다는 점이 밝혀졌다. 이러한 연구 결과는 호텔예약 앱이 가격 정보의 명확성과 비교의 용이성을 향상시켜야 하며, 사용자 맞춤형 서비스와 신속한 반응으로 소비자의 혼란과 피로감을 경감시키는 전략이 필요함을 제시한다. 이 연구는 호텔예약 분야의 이해와 지식을 확장하고 실질적인 문제의 해결 방안을 마련하는 데 있어 중요한 기여를 할 것으로 기대된다. In China, the rapid expansion of mobile hotel reservation apps has led to an information overload, resulting in pricing complexity, user confusion, and subsequent booking delays. In the context of mobile hotel booking app services in China, this study empirically examined the relationships between price complexity, consumer confusion, shopping fatigue, and purchase delay. Additionally, it also tested the mediating effect of shopping fatigue and both the moderating and the moderated mediation effects of reservation experience on these relationships. To this end, an online survey targeting Chinese consumers with an experience of booking hotels using mobile apps in China was carried out, and a total of 305 valid responses were analyzed. The key findings are as follows. Firstly, both the price complexity and consumer confusion exerted positive impacts on shopping fatigue and purchase delay. Secondly, shopping fatigue positively impacted purchase delay. Furthermore, shopping fatigue partially mediated the relationship between price complexity, consumer confusion, and purchase delay. Finally, reservation experience significantly influenced the relationship between consumer confusion and purchase delay, serving as a moderating factor and as a moderated mediating factor. The findings of this study can help enrich the understanding and knowledge base in the hotel reservation domain and devise practical and effective solutions to tackle related challenges.
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Shu-ting Liu,Chao Zhan,Yun-jing Ma,Chao-yang Guo,Wei Chen,Xiao-ming Fang,Lei Fang 한국한의학연구원 2021 Integrative Medicine Research Vol.10 No.-
Background There are several effective complementary and integrative therapies for patients with severe COVID-19. The trial aims to evaluate the efficacy and advantages of the qigong exercise and acupressure rehabilitation program (QARP) for treating patients with severe COVID-19. Methods A total of 128 patients with COVID-19 aged 20 to 80 years were recruited and randomly allocated in a 1:1 ratio to receive QARP plus standard therapies or standard therapies alone. QARP consisted of acupressure therapy and qigong exercise (Liu Zi Jue). The primary outcome was measured with the modified Medical Research Council (mMRC) dyspnea scale, and the secondary outcomes included the modified Borg dyspnea scale (MBS), fatigue Scale-14 (FS-14), patient health questionnaire-9 scale (PHQ-9), duration of respiratory symptoms, and vital signs. Results In total, 128 patients completed the clinical trial. The QARP group and standard therapies group showed significant improvements in vital signs (except blood pressure) and clinical scales compared with baseline (p<0.05). The QARP group also showed more significant improvement in the mMRC dyspnea scale (-1.8 [-2.1, -1.6], p=0.018) and modified Borg dyspnea scale (-3.7 [95% confidence intervals (CI) -4.3, -3.1], p=0.045). The duration of cough was 14.3 days (95% CI 12.6, 16.1, p=0.046), and the length of hospital stay was 18.5 days (95% CI 17.0, 20.0, p=0.042) in the QARP group, both of which were significantly reduced compared with the standard therapies group (p<0.05). Conclusion QARP plus standard therapies improved lung function and symptoms such as dyspnea and cough in patients with severe COVID-19 and shortened the length of hospital stay. Therefore, QARP may be considered an effective treatment option for patients with severe COVID-19. Trial registration Clinical Research Information Service Identifier: ChiCTR2000029994 Background There are several effective complementary and integrative therapies for patients with severe COVID-19. The trial aims to evaluate the efficacy and advantages of the qigong exercise and acupressure rehabilitation program (QARP) for treating patients with severe COVID-19. Methods A total of 128 patients with COVID-19 aged 20 to 80 years were recruited and randomly allocated in a 1:1 ratio to receive QARP plus standard therapies or standard therapies alone. QARP consisted of acupressure therapy and qigong exercise (Liu Zi Jue). The primary outcome was measured with the modified Medical Research Council (mMRC) dyspnea scale, and the secondary outcomes included the modified Borg dyspnea scale (MBS), fatigue Scale-14 (FS-14), patient health questionnaire-9 scale (PHQ-9), duration of respiratory symptoms, and vital signs. Results In total, 128 patients completed the clinical trial. The QARP group and standard therapies group showed significant improvements in vital signs (except blood pressure) and clinical scales compared with baseline (p<0.05). The QARP group also showed more significant improvement in the mMRC dyspnea scale (-1.8 [-2.1, -1.6], p=0.018) and modified Borg dyspnea scale (-3.7 [95% confidence intervals (CI) -4.3, -3.1], p=0.045). The duration of cough was 14.3 days (95% CI 12.6, 16.1, p=0.046), and the length of hospital stay was 18.5 days (95% CI 17.0, 20.0, p=0.042) in the QARP group, both of which were significantly reduced compared with the standard therapies group (p<0.05). Conclusion QARP plus standard therapies improved lung function and symptoms such as dyspnea and cough in patients with severe COVID-19 and shortened the length of hospital stay. Therefore, QARP may be considered an effective treatment option for patients with severe COVID-19. Trial registration Clinical Research Information Service Identifier: ChiCTR2000029994
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Liu, Fang,Ma, Ni,Xia, Fang-Bo,Li, Peng,He, Chengwei,Wu, Zhenqiang,Wan, Jian-Bo The Korean Society of Ginseng 2019 Journal of Ginseng Research Vol.43 No.1
Background: Ginsenosides with less sugar moieties may exhibit the better adsorptive capacity and more pharmacological activities. Methods: An efficient method for the separation of four minor saponins, including gypenoside XVII, notoginsenoside Fe, ginsenoside Rd2, and notoginsenoside Fd, from Panax notoginseng leaves (PNL) was established using biotransformation, macroporous resins, and subsequent preparative high-performance liquid chromatography. Results: The dried PNL powder was immersed in the distilled water at $50^{\circ}C$ for 30 min for converting the major saponins, ginsenosides Rb1, Rc, Rb2, and Rb3, to minor saponins, gypenoside XVII, notoginsenoside Fe, ginsenoside Rd2, and notoginsenoside Fd, respectively, by the enzymes present in PNL. The adsorption characteristics of these minor saponins on five types of macroporous resins, D-101, DA-201, DM-301, X-5, and S-8, were evaluated and compared. Among them, D-101 was selected due to the best adsorption and desorption properties. Under the optimized conditions, the fraction containing the four target saponins was separated by D-101 resin. Subsequently, the target minor saponins were individually separated and purified by preparative high-performance liquid chromatography with a reversed-phase column. Conclusion: Our study provides a simple and efficient method for the preparation of these four minor saponins from PNL, which will be potential for industrial applications.
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Liu, Zejia,Li, Yinghua,Tang, Liqun,Liu, Yiping,Jiang, Zhenyu,Fang, Daining Techno-Press 2014 Smart Structures and Systems, An International Jou Vol.14 No.2
With more and more built long-term structural health monitoring (SHM) systems, it has been considered to apply monitored data to learn the reliability of bridges. In this paper, based on a long-term SHM system, especially in which the sensors were embedded from the beginning of the construction of the bridge, a method to calculate the localized reliability around an embedded sensor is recommended and implemented. In the reliability analysis, the probability distribution of loading can be the statistics of stress transferred from the monitored strain which covered the effects of both the live and dead loads directly, and it means that the mean value and deviation of loads are fully derived from the monitored data. The probability distribution of resistance may be the statistics of strength of the material of the bridge accordingly. With five years' monitored strains, the localized reliabilities around the monitoring sensors of a bridge were computed by the method. Further, the monitored stresses are classified into two time segments in one year period to count the loading probability distribution according to the local climate conditions, which helps us to learn the reliability in different time segments and their evolvement trends. The results show that reliabilities and their evolvement trends in different parts of the bridge are different though they are all reliable yet. The method recommended in this paper is feasible to learn the localized reliabilities revealed from monitored data of a long-term SHM system of bridges, which would help bridge engineers and managers to decide a bridge inspection or maintenance strategy.
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