희토류 Nitroprusside 분말들에 대한 Mo¨ssbauer 효과 연구

김형상,홍치유,김순구,백승도 東國大學校 1998 東國論叢 Vol.37 No.-

We observed the ^57Fe Mo¨ssbauer spectra at room temperature of sodium nitroprusside. Na_2[Fe(CN)_5NO)·2H_2O single crystal, europium nitroprusside, Eu_2[Fe3(CN)_5NO]·9H_2O powder, praeseodymium nitroprusside and Pr_2[Fe(CN)_5NO]·6H_2O powder which is substituted cations as rare earth element. The Mo¨ssbauer parameters, isomer shifts(I. S.). quadrupole splittings (Q.S.). full width half maxima (F.W.H.M..Γ) and Mo¨ssbauer Lamb factors in two powder samples are compared with sodium nitroprusside single crystal for analysis of the electronic properties of the materials. We have found that values of quadrupole splitting in two powder samples are positive larger than these in sodium nitroprusside single crystal and values of isomer shifts of these in three samples are equal within experimental errors. Also we found that values of Mo¨ssbauer Lamb factor in two powder samples are smaller than theses in sodium nitroprusside single crystal and theses values in two powder samples are equal within experimental errors.

Defects Evaluation of Blue Light Emitting Materials by Wet Etching and Transmission Electron Microscopy

Soon-Ku Hong,Bong-Jin Kim 한국진공학회 1998 한국진공학회 학술발표회초록집 Vol.- No.-

농경지(農耕地)로부터의 오염물질(汚染物質) 유출부하특성(流出負荷特性) - 전(全)Kjeldahl 질소(窒素) 및 전인(燐)을 중심(中心)으로-

홍성구 ( Hong Sung Ku ),권순국 ( Kwun Soon Kuk ) 한국농공학회 1989 韓國農工學會誌 : 전원과 자원 Vol.31 No.3

With an objective to provide basic information for the management and the prediction of eutrophication in lentic water Systems, total amount of Kjeldahl nitrogen(T-N) and phosphorous(T-P) from irrigated water and drained water from flooded paddies were investigated during the rice growing period of 1988. A 29.3 ha paddies near Jungnam-myun, HwaSung-gun, Gyungi Province, Korea was instrumented for measuring runoff and sampling irrigated water and drained water from paddies. The following conclusions may be drawn from the result of this study. 1. During 115 days of investigation, T-N load for paddies was 362.6kg and T-P 63.44kg.These would be converted to 12.4kg T-N/ha and 2.17kg T-P/ha, respectively. 2. The T-N and T-P loadings in different periods showed a significant difference. The 25% of T-N loading was drained soon after fertilization period and 60% was drained during the rainy season from July 5 to July 24. 3. Annual loadings from paddies could be calculated to 30kg T-N/ha/year and 52kg T-P/ha/year considering non-measurement periods. 4. After the rainy season, the nutrient loads from drained water showed much less than those from irrigated water, and it may be suggested that the paddies would act as a stabilization pond. 5. The average concentrations of nutrients at 0.9km downstream from investigated paddies were 2.02(T-N) mg/1 and 0.52(T-P) mg/1, which were 1.82(T-N) mg/1 and 0.056(T-P) mg/1 lower than those of drained water from paddies.

Effect of Dongchunghacho on Gene Expression in Aorta and Liver in ApoE -/- Mice Fed a High-fat/high-cholesterol Diet

Nam Sook Park,Han Seok Kang,Seon Ku Kim,Yong Gyun Kim,Byung Uuk Cho,Teak Soon Shin,Keun Ki Kim,Hyun Chul Park,Hong Joo Son,Hong Gu Lee,Sook Jae Sea,Hong Ja Kim,Eunju Park,Gyeong Im Jeon,Hyun Jung Lee, 한국응용곤충학회 2009 한국응용곤충학회 학술대회논문집 Vol.2009 No.05

A full genomic DNA microarray technique was employed to investigate the effects of Dongchunghacho on aortal and hepatic gene expression in apolipoprotein E knockout mice fed a high-fat/high-cholesterol diet. Male 8- week - old ApoE-/- mice were randomly divided into two groups, control(high cholesterol group; HC) and supplementation of Dongchunghacho (SD). All of the mice were fed a high-fet/high cholesterol diet with or without Dongchunghacho supplemented by 1% for 6 weeks. At first, lipid profile of the Dongchunghacho was measured by biochemical analysis. No differences were observed in serum triglyceride and total cholesterol levels between the two groups. Antigenotoxic effect of the Dongchunghacho was measured by the single cell gel electrophoresis assay (Comet assay) and quantified as % fluorescence in tail. Dongchunghacho supplementation decreased significantly leukocytic DNA damage and also there was a tendency of reduction in hepatic DNA damage in Dongchunghacho group compared with the control group. In up regulated genes in liver and aorta of the mice, genes with 0 to 2- fold difference in expression level between the two group (HD and SD) was very much more in liver than in aorta, on the contrary, those with 2-fold to 16-flod difference increased greatly rather in aorta than in liver. Also, almost the same results were observed in down regulated genes in liver and aorta between the two groups. These results suggested that supplementation of Dongchunghacho might be helpful in preventing leukocytic DNA damage induced by high fat diet, and has a more crucial roles in aortal gene expression.

Cucurbitacin D induces cell cycle arrest and apoptosis by inhibiting STAT3 and NF-κB signaling in doxorubicin-resistant human breast carcinoma (MCF7/ADR) cells

Ku, Jin Mo,Kim, Soon Re,Hong, Se Hyang,Choi, Han-Seok,Seo, Hye Sook,Shin, Yong Cheol,Ko, Seong-Gyu Springer US 2015 MOLECULAR AND CELLULAR BIOCHEMISTRY - Vol.409 No.1

<P>Breast cancer is the most common cancer for women and is a major cause of mortality in women. Doxorubicin is a generally used chemotherapy drug for breast cancer. However, multidrug resistance of breast cancer interferes with the chemotherapy. We examined whether cucurbitacin D affects doxorubicin resistance of MCF7/ADR breast cancer cells. Cell viability was measured by MTT assay. Levels of p-STAT3, p-NF-κB, IκB, and caspases were measured by Western blot analysis. Nuclear staining of Stat3 and NF-κB was measured by immunocytochemistry. STAT3 and NF-κB transcriptional activity was detected by STAT3 and NF-κB luciferase reporter gene assays. Analysis of cell cycle arrest was performed by flow cytometry. Induction of apoptosis by cucurbitacin D was measured by Annexin V-FITC/propidium iodide assay. More than 90 % of MCF7/ADR cells lived upon treatment with doxorubicin for 24 h. However, upon treatment with cucurbitacin D, cell death was more than 60 %. Co-administration of cucurbitacin D and doxorubicin induced apoptosis, and G2/M cell cycle arrest, and inhibited upregulated Stat3 by doxorubicin on MCF7/ADR cells. Additionally, cucurbitacin D led to an increase in the IκBα level in the cytosol and a decrease in the p-NF-κB level in the nucleus. Finally, cucurbitacin D inhibited translocation of Stat3 and NF-κB and decreased transcriptional activity in the nucleus. Cucurbitacin D decreases cell proliferation and induces apoptosis by inhibiting Stat3 and NF-κB signaling in doxorubicin-resistant breast cancer cells. Cucurbitacin D could be used as a useful compound to treat adriamycin-resistant patients.</P>

Nanostructural analysis of trabecular bone

Hong, Sun Ig,Hong, Soon Ku,Kohn, David H. Springer-Verlag 2009 Journal of materials science, Materials in medicin Vol.20 No.7

Trichosanthes Radix의 추출액이 적출 동물 평활근의 장력에 미치는 효과

구자란,신홍기,김기순,김복련 한양대학교 의과대학 1993 한양의대 학술지 Vol.13 No.1

The extract of Trichsanthes radix (TRE) is a complex of active principles prepared from the root of Trichosanthes Kirlowii, known component of which include saponin, trichsanthin, palmitic acid, lignoleic acid, cerotic acid, montamic acid, melissic acid and polysaccharides etc. The present study was carried out to explore effects of TRE and its fractions (TRE1-5, TRE-3BS, TRE-3-ES $ TRE-3-EP) on the isolated vascular smooth muscle. Norepinephrine-induced contraction of the isolated rabbit aorta were relaxed almost completely by TRE-3 (0.1mg/ml) or TRE-4 (0.5mg/ml), while no significant changes in vascular tension were produced following superfusion of the vascular preparations with TRE-1, TRE-2 or TRE-3 solutions. Subfractions of TRE-3 (TRE-3Bs, TRE-3-Es & TRE-3-Ep) also relaxed norepinephrine-precontracted rabbit and rat aortic rings. In the rat aortic preparations the vascular relaxant effect of TRE-3 and TRE-4 were not affected either by removal of the ensothelium or by methylene blue. High potassium (30 mM)induced contraction was almost completely relaxed by .01mg/ml TRE-3 in the isolated rat aorta, whereas approximately 70% of potassium-induced contraction was relaxed by 0.5mg/ml TRE-3 in the rabbit aortic preparation. In the rat aortic preparations pretreated with raynodine (3×10 M) and caffeine (2×10 M), vascular relaxing effect of TRE3-was markedly abolished. The results suggest that TRE relaxes the isolated rabbit and rat aortas by a mechanism that inhibit Ca release from the intracellular calcium storage sites.

Endovascular Treatment in Patients with Persistent Internal Carotid Artery Occlusion after Intravenous Tissue Plasminogen Activator: A Clinical Effectiveness Study

Hong, Jeong-Ho,Sohn, Sung-Il,Kang, Jihoon,Jang, Min Uk,Kim, Beom Joon,Han, Moon-Ku,Park, Tai Hwan,Park, Sang-Soon,Lee, Kyung Bok,Lee, Byung-Chul,Yu, Kyung-Ho,Oh, Mi Sun,Cha, Jae Kwan,Kim, Dae Hyun,Lee S KARGER AG 2016 Cerebrovascular Diseases Vol.42 No.5

<P>Background: There has been no large-scale trial comparing endovascular treatment (add-on EVT) after intravenous tissue plasminogen activator (IV tPA) and IV tPA alone in acute ischemic stroke (AIS) caused by internal carotid artery occlusion (ICAO). We aimed at investigating the effectiveness and safety of add-on EVT after IV tPA in AIS patients with ICAO. Methods: Between March 2010 and March 2013, 3,689 consecutive ischemic stroke patients who were hospitalized within 4.5 h of onset were identified using a prospective stroke registry at 11 centers in Korea. Among them, patients with persistent ICAO after receiving IV tPA and whose 3-month modified Rankin Scale (mRS) was available were finally enrolled. A propensity score analysis with inverse-probability of treatment weighting was used to eliminate baseline imbalances between those receiving add-on EVT and IV tPA alone. Results: Among 264 patients enrolled in this study (mean age 71.4; male 56.4%; median National Institute of Health Stroke Scale score 15), 117 (44.3%) received add-on EVT. The add-on EVT group had a higher frequency of favorable outcome on the mRS <= 2 (35.0 vs. 18.4%; adjusted OR (aOR) 2.79; 95% CI 1.66-4.67) and lower mortality (17.9 vs. 35.4%; aOR 0.24; 95% CI 0.13-0.42) at 3 months, when compared to the IV tPA-alone group. Add-on EVT did not significantly increase the risk of symptomatic hemorrhage (5.1 vs. 4.1%; aOR 1.01; 95% CI 0.37-2.70). The rate of successful recanalization (thrombolysis in cerebral infarction grade >= 2b) in the add-on EVT group was 69.2%. Conclusions: Compared to an IV tPA alone, add-on EVT can improve clinical outcomes in patients with symptomatic ICAO within 4.5 h of onset without a significant increase of symptomatic hemorrhage. (C) 2016 S. Karger AG, Basel</P>

Responses of Plasma IGF-1, IGFBPs and Hepatic GH Receptor to Growth Hormone Releasing Peptides (GHRP)-2 Administration and Energy Level in Wethers

Hong Gu Lee(이홍구),Young Cheng Jin(김영성),Hisashi Hidari,Yun Jaie Choi(최윤재),Seon Ku Kim(김선구),Teak Soon Shin(신택순),Byung Uuk Cho(조병욱),Yong Gyun Kim(김용균),Keun Ki Kim(김근기),Hong Joo Son(손홍주),Sang Mong Lee(이상몽) 한국생명과학회 2008 생명과학회지 Vol.18 No.7

본 연구는 정상으로 단백질을 급여한 거세면양에 있어서 에너지 첨가가 GHRP-2투여에 대한 혈장 IGF-1 및 IGFBPs에 대한 반응과, 고에너지 급여에 따른 GHRP-2투여가 hepatic GH 수용체에 미치는 영향을 검증하기 위하여 실시하였다. 시험 결과 HENP (CP 0.34 ㎏, TDN 1.83 ㎏/day DM intake)처리기간 동안 혈장 IGF-1 과 39-42kDa IGFBP-3수준은 LENP (CP 0.32 ㎏, TDN 0.87 ㎏/day DM intake)기간에 비하여 높게 나타났으나 (P<0.05), 혈장 34 kDa IGFBP-2와 24 kDa IGFBP-4는 영양처리에 의해 영향을 받지 않았다. 각 영양처리 기간동안 GHRP-2 (12.5 μg/㎏ body weight/day)투여는 혈장 GH 반응이 촉진되었으며(P<0.05), 혈장 GH 평균 함량과 AUC증가에 있어서는 LENP처리 기간에 비하여 HENP처리기간에서 유의적으로 높게 나타났다(P<0.01). 특히 HENP에서 7일간 GHRP-2투여에 의한 일중 혈장 IGF-1 변화양상을 조사한 결과 투여 2, 6 및 7일에서 뚜렷한 증가양상을 보였다(P<0.05). 이에 반하여 LENP에서는 오직 투여 3일째에서 Saline구에 비하여 유의적인 증가를 확인하였다(P<0.05). IGFBPs의 ligand blotting 결과 HENP구에서 혈장 39-43 kDa IGFBP-3의 수준의 증가가 투여 6일과 7일에서 관찰되었으나 혈장 IGFBP-2수준은 두 영양처리시기에서 유의적인 차이를 관찰하지 못했다. 아울러 HENP구에 있어서 간세포막에 <SUP>125</SUP>I-oGH의 결합력을 측정한 결과 GHRP-2투여에 의한 영향은 관찰되지 않았다. 이와 같은 결과는 거세면양에 있어서 단백질과 에너지 사이의 영양적 균형은 내인성 GH/IGF-1 axis는 물론 혈장 IGFBP-3수준의 변화에 영향을 미치고 있음을 시사한다. The purpose of this study was to determine the effect of energy supplement on responses of plasma insulin-like growth factor (IGF)-1 and IGF binding proteins (IGFBPs) to growth hormone-releasing peptide-2 (GHRP-2) administration in normal protein-fed wethers, and to observe the effect of GHRP-2 treatment on hepatic growth hormone (GH) receptor in well-fed wethers. Plasma IGF-1 and 39-42 kDa IGFBP-3 during the HENP (CP, crude protein 0.34 and TDN, total digestible nutrients 1.83 ㎏/day DM, dry matter intake) treatment period were higher than in the LENP (CP 0.32 ㎏ and TDN 0.87 ㎏/day DM intake) period (P<0.05). The response of GH was stimulated by GHRP-2 (12.5 ㎍/㎏ body weight/day) administration during both of the feed treatment periods (P<0.05). The area under curve (AUC) increment and average concentration of GH (0-180 min) with GHRP-2 administration was higher during HENP treatment than LENP treatment (P<0.01). During the HENP treatment period from day 1 to day 7 of twice daily GHRP-2 treatment, the plasma IGF-1 increment was increased on days 2, 6 and 7 of GHRP-2 administration (P<0.05). On the basis of ligand blotting, the proportions of plasma 39-43 kDa IGFBP-3 during the HENP treatment period only showed a significant difference on days 6 and 7 with GHRP-2 administration. No significant difference in the specific binding of 125I-labeled oGH to hepatic membranes was detected between the saline and GHRP-2 treatments of the HENP-fed wethers. These results suggest that the nutritional balance between energy and protein may affect the endogenous GH / IGF-1 axis as well as plasma IGFBP-3 levels.

당뇨병성 족부궤양의 임상적 관찰

구본정,최대은,정진옥,나소영,이효진,홍우정,김군순,조영석,김도희,송민호,노흥규,김영건 대한당뇨병학회 2002 임상당뇨병 Vol.3 No.3

연구배경: 당뇨병의 유병률이 증가함에 따라 당뇨병의 만성합병증 또한 증가하고 있다. 당뇨병성 족부궤양은 하지절단의 원인이 되는 무서운 만성 합병증이다. 이러한 당뇨병성 족부궤양의 관리에는 예방 및 즉각적인 치료가 필수이다. 저자들은 당뇨병성 족부병변이 발생한 환자들의 임상적 특성을 알아보고, 예방과 적절한 치료지침을 세우는데 도움이 되고자 본 연구를 수행하였다. 방법: 2001년 1월부터 12월까지 충남대학교병원 내분비병동에 당뇨병성 족부궤양으로 입원한 총 73명의 환자들 대상으로 후향적으로 의무기록을 검토하였다. 결과 : 1. 당뇨병성 족부궤양으로 입원한 환자는 60대(38.4%)가 가장 많았으며, 남자(79.5%)에서 보다 많았다. 2. 당뇨병성 족부궤양으로 입원한 환자의 평균 당뇨병 유병기간은 14.6년이었다. 3. 족부궤양의 발생원인은 잘 모르는 경우(72.6%)가 대부분이었다. 4. 균배양검사에서는 Staphylococcus가 가장 많이 동정되었다. 5. Wagner법에 의한 족부궤양의 중등도 분류에서는 3등급(41.4%)이 가장 많았다. 6. 총 73명의 환자 중 63명(86.3%)은 내과적으로 성공적으로 치료하였으며, 9명의 환자는 하지 절단술을 시행받았다. 내과적으로 치료한 환자의 재원기간(약 25.1일)이 외과적인 경우(약 80.4일)보다 짧았다. 7. 혈관조형술은 20명(27.4%)의 환자에서 시행했으며, 16명(80%)에서 양성이었다. 8. 골주사검사는 62명(84.9%)의 환자에서 시행되었고, 양성인 경우는 35명(56.5%)이었다. 9. 족부절단의 위험인자 분석에서는 궤양의 정도와 관련이 있었으며(p=0.014), 당뇨병의 유병기간이나, 혈관조형술의 결과, 골주사 검사 결과 등과는 관련이 없었다. 결론 : 당뇨병성 족부궤양은 일단 한 번 발병하면 입원기간도 길고 치료가 어려운 질환이다. 발 관리에 대한 조기교육과 엄격한 혈당조절을 통한 예방만이 가장 최선의 방법이라 하겠다. Background : The diabetes mellitus is increasing now. So chronic complication of diabetes is being a big problem in the world. The Diabetic foot ulcer is one of the serious chronic complication because they can cause lower limb amputation. Prevention and prompt treatment is important in management of diabetic foot ulcer. We plan to study to evaluate clinical characteristics and to help the making a prevention policy and treatment plan in diabetic patients with foot ulcer. Methods : We reviewed medical record and checked the results of bacteriologic culture, angiography, and bone scan in diabetic patient with foot ulcer admitted to CNUH during last one year. Results : 1. Diabetic foot ulcers were occurred most frequently in 7th decades of age (38.4%) and more prevalently in man (79.5%). 2. The mean disease duration of diabetes who had a foot ulcer was about 14.6 years. 3. The most common causative event of diabetic foot ulcer was unknown (72.6%). 4. Wound culture was done in 53 (72.6%) diabetic patients with foot ulcer and the most common organism was Staphylococcus. 5. We graded diabetic foot ulcer by Wagner grading system and most common ulcer was grade 3(41.4%). 6. We was treated medically in 63(86.3%) patients and surgically in 9 patients. The duration of hospital stay was longer in surgically(80.4 days) treated patients than in medically(25.1 days). 7. Angiography was done in 20(27.4%) patients and positive result was 80%. 8. 3-phase bone scan was done in 62(84.9%) patients and positive results was 56.5% 9. Risk factors for amputation was ulcer grade and not related to duration of diabetes, angiographic findings and bone scan results. Conclusion : Diabetic foot ulcer is serious disease. Education for foot care and strict glycemic control should be necessary to prevent diabetic foot ulcer.