한방 이화주의 미백 및 피부 주름 개선 효과

이상진(Sang-Jin Lee),권이영(Yi-Young Kwon),조성원(Sung-Won Cho),권희숙(Hee-Suk Kwon),신우창(Woo-Chang Shin) 한국식품영양과학회 2013 한국식품영양과학회지 Vol.42 No.4

본 연구에서는 한방이화주의 피부 생리기능 활성을 알아보기 위해 70% EtOH 추출물의 피부 미백, 주름 개선 및 항염증 효과를 조사하였다. HEE은 tyrosinase 활성 억제 및 tyrosine을 기질로 melanin이 형성되는 pathway에 관여하는 주요한 인자인 TRP-1과 TRP-2를 저해하는 작용 기전을 통해 피부 색소침착의 주요 원인 물질인 melanin 생합성을 농도 의존적으로 저해하는 것을 확인하였다. HEE은 피부진피 내 피부 탄력을 유지하는 elastin을 분해하는 효소인 elastase의 활성을 저해하였고, 피부의 keratinocyte가 생성분비하는 MMP-2와 MMP-9의 단백질 발현과 proteolytic 활성을 억제하여 노화에 따른 피부 주름 생성 억제할 수 있는 가능성을 확인하였다. 또한 세포 독성 없이 LPS에 의해 유도된 염증 반응을 50% 저해하는 HEE의 농도(IC50)는 24.9 μg/mL이며, 50 μg/mL 농도로 처리하였을 때 염증 반응 저해 효과가 70%로 높은 효과를 가지는 것을 확인하였다. 이상의 결과를 종합하면 HEE의 피부 미백, 주름 개선 및 항염증에 우수한 효과를 나타내고 있으므로 기능성 화장품의 주요한 소재로 이용 가치가 높을 것으로 사료된다. Extrinsic skin aging is characterized by the loss of skin tone and resilience, irregular pigmentation, and deep wrinkles. The aim of this study was to investigate the effects of Ehwa Makgeolli containing oriental herbs (Glycyrrhiza uralensis Fisch., Lycium chinense MILL., Morus alba L., and Saururus chinensis Baill) on skin whitening and wrinkling in human skin cells. We prepared Makgeolli extracts (HEE) with 70% ethanol. HEE significantly inhibited in vitro mushroom tyrosinase activity and reduced the cellular and secreted melanin content of mouse melanoma melanocytes (B16F1 cells). HEE down-regulated the protein expression of tyrosinase related protein (TRP)-1/-2, a key player in melanogenesis. Treatment with HEE in human keratinoctyes (HaCaT cells) inhibited the proteolytic activities of matrix metalloproteinase (MMP)-2/-9 in a dose-dependent manner and dramatically reduced the expression of MMP-2/-9. In addition, HEE attenuated lipopolysaccharide (LPS)- induced nitric oxide production in murine macrophages (RAW264.7 cells). These results indicate that HEE may be a great cosmeceutical ingredient for its whitening, anti-wrinkle, and anti-inflammatory effects.

입제의 수면처리에 의한 주요수도해충의 동시방제

최승윤,배상희,송유한,Choi Seung Yoon,Bae Sang Hee,Song Yoo Han 한국응용곤충학회 1970 한국식물보호학회지 Vol.9 No.1

본 시험은 유기인계 살충제인 Terracur P입제 및 Lebaycid 입제와 유기염소계 살충제인 상감마입제$(\gamma-BHC 입제)$를 수면시용 하였을때 수도요해충인 이화명충, 끝 동매미충, 애멸구 및 흰둥멸구에 대한 동시방제가능성과 방제적기를 구명하고 약제간의 방제효과를 비교코저 실시하였다. 얻어진 시험결과는 다음과 같다. 1) 약제처리후 부화직후 이화명충 접종 시험에 있어서 상감마와 Terracur P는 높은 살충율을 보였으나 Lebaycid의 살충율은 낮았다. 約 $50\%$ 살충력 잔효일수에 있어서 상감마는 8-14日이었고 Terracur P는 6일간이었다. 2) 당초 계획된 방제적기에 관한 정보는 얻을 수 없었다. 즉 주어진 일간격이 좁아 유의한 차를 얻을 수 없었다. 3) 1,2화기 이화명충에 대한 방제효과는 Terracur P=상감마>Lebaycid의 순이었다. 4) Terracur P와 Lebaycid 입제의 처리는 끝동매미충의 방제효과가 좋았으나 상감마$(\gamma-BHC 입제)$의 방제효과는 기대할 수 없었다. 끝동매미충에 대한 Terracur P와 Lebaycid입제의 잔효력은 30일 이상이었다. 5) 애멸구와 흰등별구에 대한 시험은 충의 서식밀도가 낮기 때문에 약제간 방제효과를 얻을 수 없었다. 6) 공시약제중 Terracur P와 Lebaycidd입제는 이화명충과 끌동매미충의 동시방제제로서 사용할 수 있을 것 같다. This experiment was conducted to find out the simultaneous control effect of granulated insecticides on the several major rice insect pests in Korea. The granulated insecticides used were Terracur P$5\%$ (one of the phosphoric insecticides), Lebaycid $5\%$, and Sang-gamma (gamma-BHC) $6\%$. Those insecticides were broadcasted by hand on the paddy field at the ratio of 3kg. in June and 4kg. in August per 10 a, and the date of insecticidal application was based on the maximum moth occurrence of the rice stem borer in 1969. The rice insect pests tested in this study were rice stem borer (Chilo suppressalis W.), green rice leafhopper (Nephotettix bipunctatus cincticeps U.), smiller brown planthopper (Laodelphax striatellus F.), and white back planthopper (Sogotella furcifera H.). For the study of residual effectiveness of the insecticides, the rice stem borer larvae just hatched from the eggs were installed on the sheath of rice plants collected from the paddy field after insecticidal treatment, and the mortality was made at 48 hours after installation. High mortality was observed in Terracur P and Sang-gamma treatments and low mortality in Lebaycid treatment. The duration of residual effects giving about 50% mortality was about 14 days in Sang-gamma and about 6 days in Terracur P treatments. The study on the effectiveness of the insecticides to the rice stem borer in the paddy field was made by the number of dead blades and white heads of the rice plants injured by the larvae, and the number of survival larvae in the straws after crop harvest. The order of insecticidal effectiveness to the rice stem borer was Terracur P=Sang-gamm>Lebaycid. The study on the effectiveness of the insecticides to the leafhoppers and planthoppers in the paddy field was made by the population density by sweeping (5 times) at the given day intervals after treatments. Terracur P was highly effective for the control of the green rice leafhoppers and Lebaycid was moderately effective, but Sang-gamma seemed not to be effective. The effectiveness of the insecticides tested to the planthoppers was not clear, and it seemed to related with the low insect population density. Study on the determination of proper timing of insecticidal application was not also made clear, and it seemed to be short in the range of day intervals in the insecticidal application. For the study on the control effects of the rice insect pests, rice yield per 100 hills per plot was observed. There was no statistically significant difference among the treatments in rice yields, but the order of yield (Terracur P>Sang-gamma>Lebaycid>Control) was paralleled with the tends of control effects above mentioned. In a view point of simultaneous control of the rice stem borers and green rice leafhoppers by the application of granulated insecticides, Terracur P and Lebaycid were more effective than Sang-gamma which have been used.

산포도와 머루중의 Polyphenol Oxidase에 관한 연구¹

성찬기,박희중,임흥빈,조성희 中央大學校 基礎科學硏究所 1997 基礎科學硏究所 論文集 Vol.11 No.-

Polyphenol oxidase was partially purified from fresh vitis flexuosa and vitis amurensis and its characteristics were studied. the experimental results were summarized as follows. 1.The enzyme activities were increased during the fruit developement gradually in two sample. 2.The optimum pH and temperature for activity were 5.5 and 30℃, respectively. 3.The enzyme was active with o-phenols and trihydroxyphenol, but inactive toward m-diphenols and monophenols. 4.The ?? value of the enzymes was 15.4 mM in vitis flexuosa and 12.5 mM in vitis amurensis with catechol as substrate. 5.Inhibitors sudies indicated that sodium diethyl-dithiocarbamate, L-cysteine, sodium bisulfite, L-ascorbic acid and 2-mercaptoeyhanol were the most potent. 6.The enzyme activity increament by ?? was much better than those by the other metal ions.

법치경제 개혁의 길

좌승희, 조성봉(Sung-Hee JWA),Sung Bong CHO 한국경제연구원 2004 한국경제연구원 연구보고서 Vol.2004 No.-

Sung-Hee JWA and Sung Bong CHO The establishment of the rule of law in the social and economics spheres is fundamental towards effective and practical reform of a country’s legal and institutional environment. Such reform must take place under the rule of law, and not through human or administrative discretion. We claim that establishing ‘a law-governed economy,’ which is defined as the operation of an economy under the rule of law, is an indispensible feature for Korea’s proper economic reform. In Korea, many factors presently hinder the establishment of a law-governed economy, of which the most important are the persistent government intervention into markets, the government’s excessive discretionary power, the weak check and balance among the three government branches, the absence of regulatory bodies’ independence, the unclear division of labor among government bodies, and the ‘egalitarian trap’ embedded in the Korean culture. We argue in this paper that political reform is a prerequisite to a law-governed economy. There should be a conscious effort to remove entry and exit barriers in political markets, as well as reduce significantly its high-cost structure. Through such a process, and by improving the country’s economic institutional environment, more effective economic reform, especially of corporations, will then become possible. Regarding corporate sector reform, there is a need to establish a proper market disciplinary system by strengthening the monitoring roles of various market participants, thus replacing the existing regulatory framework that aims to directly control corporate behavior.

Monascus anka 코오지를 이용한 간장의 품질특성

서성희,박성오 서울여자대학교 자연과학연구소 1990 자연과학연구논문집 Vol.1 No.-

Aspergillus sojae 코오지와 Monascws anka 코오지의 비율을 달리한 간장(I구 : Aspergillus sojae 코오지만 사용, II구 : Aspergillus sojae 코오지 85% + Monascus anka 15%, III구 : Aspergillus sojae 코오지 70% + Monasaus anka 30%)의 일반분석, 효소력, 색도, 관능검사등을 숙성과정 중에 측정한 결과는 다음과 같다. 1. 총질소와 아미노태질소는 차차 증가하여 숙성 90일에 각각 840.5~904.5mg%, 403~448mg%였으며 시험구별로는 II구, III구, I구 순이였다. 암모니아태질소는 숙성중 계속 증가하였다. 유리아미노산은 glutamic acid와 aspartic acid가 비교적 많이 검출되었다. 시험구별로는 II구, III구, I구 순이였다. 2. 환원당과 총당은 숙성 60일에 최대치를 나타냈고 차차 감소하여 숙성 90일에는 각각 3.78~4.50%, 4.58~5.30%였으며 시험구별로는 I구, II구, III구 순이였다. 3. pH는 숙성 50일에 가장 낮았으나 적정산도는 가장 높았다. 시험구별로 pH는 III구, I구, I구 순이였고 적정산도는 I구, II구, III구 순이였다. 4. 순고형분과 비중은 차차 증가하여 숙성 90일에는 각각 8.76~9.44%, 1.194~1.202 Bé였다. 5. 색도는 숙성 90일에는0.37~0.61이였으며 시험구별로는 III구, II구, I구 순이였다. 6. 관능검사 결과 세 시험구 사이에 유의적인 차이가 없었다. Each group of soy sauce was prepared with Aspergillus sojae koji alone(type I), with the mixture of 85% of Aspergillus sojae koji and 15% of Monascus anka koji (type II), and with the mixture of 70% of Aspergillus sojae koji and 30% of Monascus anka koji (type III). Total nitrogen, amino nitrogen and ammoniacal nitrogen were continuously increased until 90 days after preparation. As a free amino acid, aspartic acid and glutamic acid were shown highest content. After 60 days of preparation, reducing sugar and total sugar were showed higtest level, 4.04~4.92% and 4.66~5.51%, respectively, and then the content was slightly decreased until 90 days after preparation. After 50 days of preparation, pH was lowest with 4.94~5.26 and titrable acidity was highest with 5.71~5.90ml. Pure extract and specific gravity were reached to 8.76~9.44%, 1.194~1.202Bé. respectively at 90 days after preparation. Color was increased with aging time to 0.37~0.61 at 90 days after preparation. There was no remarkable difference for sensory evaluation among three types.

고양이 위 평활근에서 KCI에 의한 근수축에 대한 Protein Kinase의 조절

심상수,김승록,심혜선,이덕주,한상준,윤신희,조양혁,김명석 大韓消化器病學會 1998 大韓消化器病學會誌 Vol.32 No.3

투사형 전조등의 광학적 분석

성희진,홍사용,류지욱 公州大學校 基礎科學硏究所 1999 自然科學硏究 Vol.8 No.-

투사형 전도등의 특성을 조사하기 위해 광선 추적법으로 광학계를 분석하고 그 결과를 포물경 전도등과 비교하였다. 포물경 전도등의 경우 광학계가 단순한 장점이 있지만 광원의 일부분 만을 반사하며 실제 광원의 크기와 위치를 고려할 때 높은 수차가 불가피 했다. 투사형 전도등은 여러개의 광학부품 사용하는 복잡함과 전체적 길이가 길어지는 단점이 있지만 포물경 전도등 보다 40%이상의 빛을 더 활용하며 전체적인 수차도 상당히 줄일 수 있었다. 그러나 투사형 전도등 광학계의 성능은 비구면 렌즈의 형태에 매우 민감한 것으로 나타났다. To investigate characteristics of the projection type headlights for automobiles, we used ray tracing methods and compared the results with those from parabolic type headlights. The parabolic type headlights are simple but have two limits: first, they require large apertures. To reflect 50% of a source light, the aperture must be 4 times of focal length and to have more reflected light, apertures must grow geometrically. second, a parabolic mirror have large aberration when a source is away from a focus, since output of a headlight cannot be divergent, a source should locate far side from a mirror and moreover a light source has a finite size, large aberration is unavoidable. The projection type headlights have many optical components and longer depth compared with parabolic types, but their apertures can be made small. Advantage of using a ellipsoidal mirror is to reduce beam divergence by half and displace a focal position to another focal point, so that spherical aberration free and short focal length aspherical lens can be placed in proper position. In our study, we adopt a commercially available aspheric lens and the results were quite good compared with those from parabolic types but not optimal since output experienced total reflection at an aspheric lens. We found to improve performance of headlights, proper design of an aspheric lens was crucial.

3DFT-CISS를 이용한 감각신경성 난청 환자의 MBI

김성원,여상원,김상후,김종수,조재흥,이동희,이승균 대한이비인후과학회 1999 대한이비인후과학회지 두경부외과학 Vol.42 No.7

한국인 파킨슨병에서 Monoamine Oxidase B 유전자와 Cytochrome P450IID6 유전자의 다형성

이용희,임정근,이상도,이영재 대한신경과학회 1999 대한신경과학회지 Vol.17 No.1

종교적 상징성을 중심으로 본 현대 도시사찰 사례 연구

곽태희,양상현 순천향대학교 산업기술연구소 2004 순천향 산업기술연구소논문집 Vol.10 No.2

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