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- 저자 : S.H. Jang (검색결과 5,389 건)
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Jang, J.S.,Gyu Kim, H.,Borse, P.H.,Lee, J.S. Pergamon Press ; Elsevier Science Ltd 2007 International journal of hydrogen energy Vol.32 No.18
A process of simultaneous hydrogen production and H<SUB>2</SUB>S removal has been investigated over a highly active composite photocatalyst made of bulk CdS decorated with nanoparticles of TiO<SUB>2</SUB>, i.e. CdS(bulk)/TiO<SUB>2</SUB>. The photocatalytic activity was evaluated for hydrogen production from aqueous electrolyte solution containing H<SUB>2</SUB>S dissolved in water or alkaline solution under visible light irradiation. The rate of hydrogen production from the H<SUB>2</SUB>S-containing alkaline solution was similar to the rate obtained from photocatalytic hydrogen production from water containing sacrificial reagents (Na<SUB>2</SUB>S+Na<SUB>2</SUB>SO<SUB>3</SUB>) in the similar concentration. The isotope experiment was carried out with D<SUB>2</SUB>O instead of H<SUB>2</SUB>O to investigate the source of hydrogen from photocatalytic decomposition of H<SUB>2</SUB>S dissolved in H<SUB>2</SUB>O or alkali solution under visible light. Hydrogen originated from both H<SUB>2</SUB>S and H<SUB>2</SUB>O when the reaction solution contained H<SUB>2</SUB>S absorbed in alkaline water.
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Jang, J.W.,Ko, S.Y.,Byoun, M.S.,Sung, H.W.,Lim, C.S. Elsevier Science 2015 Journal of clinical virology Vol.73 No.-
Background: Rapid identification and subtype determination of influenza virus is important in managing infected patients. Rapid influenza diagnostic tests (RIDTs) are widely used in this manner, but most can only detect influenza A and B viruses without subtyping. A new RIDT, GENEDIA Multi Influenza Ag Rapid Test (GENEDIA), was developed for detection of influenza A and B viruses and also subtyping of influenza A to H1, H3, H5 which has not been possible with other RIDTs. Objectives: Assess the performance of GENEDIA. Study design: Nasopharyngeal swabs were collected from 274 clinically suspected patients (influenza A/H1N½009 (n=50), influenza A/H3 (n=50), influenza B (n=73) and influenza-negative (n=101)) and analyzed with the real-time RT-PCR, GENEDIA, SD Bioline Influenza Ag, and Alere BinaxNow Influenza A&B Card. Also, 46 fecal specimens (H5N2 (n=3), H5N3 (n=3)) of spot-billed duck were analyzed with RT-PCR and GENEDIA. Results: Compared to real-time RT-PCR, the sensitivities of GENEDIA, SD Bioline Influenza Ag, and Alere BinaxNow Influenza A&B Card were 73.0%, 57.0%, 58.0% for influenza A, respectively, and 68.5%, 65.8%, 57.5% for influenza B, respectively. Specifically, the sensitivity of GENEDIA was 70.0% for influenza A/H1N½009 and 76.0% for influenza A/H3. From the avian influenza samples, GENEDIA detected all six H5 subtype without any cross-reactions. Conclusion: The GENEDIA Multi Influenza Ag Rapid Test was sensitive in detecting influenza viruses compared with other commercial RIDTs and also useful for rapid subtype determination of influenza A.
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Jang, J.Y.,Koh, M.,Bae, H.,An, D.R.,Im, H.N.,Kim, H.S.,Yoon, J.Y.,Yoon, H.J.,Han, B.W.,Park, S.B.,Suh, S.W. Elsevier Science 2017 Biochimica et biophysica acta. Proteins and proteo Vol.1865 No.6
<P>Peroxisome proliferator-activated receptor gamma (PPAR gamma) is a member of the nuclear receptor superfamily. It functions as a ligand-activated transcription factor and plays important roles in the regulation of adipocyte differentiation, type 2 diabetes mellitus, and inflammation. Many PPAR gamma agonists bind to the canonical ligand-binding pocket near the activation function-2 (AF-2) helix (i.e., helix H12) of the ligand-binding domain (LBD). More recently, an alternate ligand-binding site was identified in PPAR gamma LBD; it is located beside the 52 loop between the helices H2 ' and H3. We reported previously that the chirality of two optimized enantiomeric PPAR gamma ligands (S35 and R35) differentiates their PPAR gamma transcriptional activity, binding affinity, and inhibitory activity toward Cdk5 (cyclin-dependent kinase 5)-mediated phosphorylation of PPAR gamma at Ser245 (in PPAR gamma 1 numbering; Ser273 in PPAR gamma 2 numbering). S35 is a PPAR gamma phosphorylation inhibitor with promising glucose uptake potential, whereas R35 behaves as a potent conventional PPAR gamma agonist. To provide a structural basis for understanding the differential activities of these enantiomeric ligands, we have determined crystal structures of the PPAR gamma LBD in complex with either S35 or R35. S35 and R35 bind to the PPAR gamma LBD in significantly different manners. The partial agonist S35 occupies the alternate site near the Omega loop, whereas the full agonist R35 binds entirely to the canonical LBP. Alternate site binding of S35 affects the PPAR gamma transactivation and the inhibitory effect on PPAR gamma Ser245 phosphorylation. This study provides a useful platform for the development of a new generation of PPAR gamma ligands as anti-diabetic drug candidates.</P>
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Kim, H. J.,Yoo, H. Y.,Jang, J. H.,Lin, H. Y.,Seo, E. Y.,Zhang, Y. H.,Kim, S. J. Springer Science + Business Media 2016 Pfl ugers Arch Vol.468 No.4
<P>Pulmonary arteries (PAs) have high compliance, buffering the wide ranges of blood flow. Here, we addressed a hypothesis that PA smooth muscle cells (PASMCs) express nitric oxide synthases (NOS) that might be activated by mechanical stress and vasoactive agonists. In the myograph study of endothelium-denuded rat PAs, NOS inhibition (L-NAME) induced strong contraction (96 % of 80 mM KCl-induced contraction (80K)) in the presence of 5 nM U46619 (thromboxane A(2) (TXA(2)) analogue) with relatively high basal stretch (2.94 mN, S(+)). With lower basal stretch (0.98 mN, S(-)), however, L-NAME application following U46619 (TXA(2)/L-NAME) induced weak contraction (27 % of 80K). Inhibitors of nNOS and iNOS had no such effect in S(+) PAs. In endothelium-denuded S(+) mesenteric and renal arteries, TXA(2)/L-NAME-induced contraction was only 18 and 21 % of 80K, respectively. Expression of endothelial-type NOS (eNOS) in rat PASMCs was confirmed by RT-PCR and immunohistochemistry. Even in S(-) PAs, pretreatment with H2O2 (0.1-10 mu M) effectively increased the sensitivity to TXA(2)/L-NAME (105 % of 80K). Vice versa, NADPH oxidase inhibitors, reactive oxygen species scavengers, or an Akt inhibitor (SC-66) suppressed TXA(2)/L-NAME-induced contraction in S(+) PAs. In a human PASMC line, immunoblot analysis showed the following: (1) eNOS expression, (2) Ser(1177) phosphorylation by U46619 and H2O2, and (3) Akt activation (Ser(473) phosphorylation) by U46619. In the cell-attached patch clamp study, H2O2 facilitated membrane stretch-activated cation channels in rat PASMCs. Taken together, the muscular eNOS in PAs can be activated by TXA(2) and mechanical stress via H2O2 and Akt-mediated signaling, which may counterbalance the contractile signals from TXA(2) and mechanical stimuli.</P>
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Hong, S. H.,Lee, S. S.,Chung, J. M.,Jung, H. s.,Singh, S.,Mondal, S.,Jang, H. H.,Cho, J. Y.,Bae, H. J.,Chung, B. Y. Springer Science + Business Media 2017 Protoplasma Vol.254 No.1
<P>Yeast peroxiredoxin II (yPrxII) is an antioxidant enzyme that plays a protective role against the damage caused by reactive oxygen species (ROS) in Saccharomyces cerevisiae. This enzyme consists of 196 amino acids containing 2-Cys Prx with highly conserved two active cysteine residues at positions 48 and 171. The yPrxII has dual enzymatic functions as a peroxidase and molecular chaperone. To understand the effect of additional cysteine residues on dual functions of yPrxII, S79C-yPrxII and S109C-yPrxII, the substitution of Ser with Cys residue at 79 and 109 positions, respectively, was generated. S109C-yPrxII and S79C-yPrxII showed 3.7- and 2.7-fold higher chaperone and peroxidase activity, respectively, than the wild type (WT). The improvement in enzyme activity was found to be closely associated with structural changes in proteins. S109C-yPrxII had increased beta-sheet in its secondary structure and formed high-molecular-weight (HMW) as well as low-molecular-weight (LMW) complexes, but S79C-yPrxII formed only LMW complexes. HMW complexes predominantly exhibited a chaperone function, and LMW complexes showed a peroxidase function. In addition, transgenic yeast cells over-expressing Cys-substituted yPrxII showed greater tolerance against heat and oxidative stress compared to WT-yPrxII.</P>
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吳秉夏,黃殷成,李炯周,李啓瑚,朴官和,張海東,徐鉉昌 서울大學校農科大學 1984 서울대농학연구지 Vol.9 No.1
澱粉으로 부터의 alcohol 醱酵能을 增進시키기 爲하여 澱粉糖化性 菌株인 Saccharomyces diastaticus와 優秀한 alcohol 醱酵性 菌株인 Saccharomyces uvarum을 母菌株로 하여 이들간의 同屬異種間 原形質融合(interspecific protoplast fusion)을 通한 優秀한 澱粉醱酵 性 alcohol 生産性 菌株를 새로이 開發할 目的에서 다음과 같은 一漣의 實驗結果를 얻었다. S. diastaticus의 醱酵液과 S. diastaticus+S. uvarum 混合醱酵液의 風味特性등을 確認하였다. 風味成分 抽出은 methylene chloride와 diethylether를 가지고 neutral flavor fraction과 acidic flavor fraction으로 나누었고 gas chromatography를 通하여 同定 및 定量하였다. Neutral flavor fraction의 경우 S. diastaticus+S. uvarum 混合醱酵液이 S. diastaticus 醱酵液보다, ester成分中에서는 ethyl acetate와 ethyl undecanoate가 더 많았고, alcohol 成分中에서는 n-propanol과 n-butanol이 더 많았다. Acidic flavor fraction의 경우 C??~C?? fatty acid가 同定 및 定量되었는데 S. diastaticus+S. uvarum 混合醱酵液이 S. diastaticus 醱酵液보다 lauric acid, caprylic acid, capric acid 含量이 두드러지게 많았다. S. diastaticus의 glucoamylase 生産性, glucoamylase의 分離 精製, 酵素力價 그리고 酵素學的 特性에서 optimum pH는 5.0, optimum temperature는 55℃ 이었다. S. diastaticus와 S. uvarum을 母菌株로 이들 간의 protoplast fusion을 위한 基礎的인 硏究로서 두 菌株의 諸特性과 protoplast調製의 最適條件을 決定하고 protoplast의 regeneration 條件의 確立을 도모하였다.두 菌의 生育曲線에서 모두 培養開始 7~8 時間만에 對數期 中期에 到達되었으므로 protoplast 調製는 이 時期의 細胞를 쓰기로 하였다. Generation time은 S. diastaticus가 1.04, S. uvarum이 1.38 時間이었다. 細胞의 크기는 S. diastaticus 44.10?㎛³, S. uvarum 99.67㎛³로 S. uvarum이 2倍나 컸다. DNA 含量은 細胞 當 S. diastaticus 44.3fg, S. uvarum 37.6fg이었다. 30% glucose 및 soluble starch에 대한 두 菌株의 ethanol 醱酵能은 glucose에 對하여 S. uvarum 11.4%, S. diastaticus 8.9% 이었고 soluble starch에 對하여는 S. diastaticus 만이 6.9%이었다. 두 菌株는 generation time, 細胞크기 및 DNA 含量 等으로 보아 diploid strain임을 알 수 있었고, 融合株 選拔을 위한 marker 로는 Sacch. uvarum의 melibiose 資化能의 차이를 利用할 수 있음을 밝혔다. Protoplast의 調製에는 β-glucuronidase와 Zymoyase를 使用하였는데 두 酵素 反應最適條件은 β-glucuronidase는 pH 8.0에서 10% 濃度의 溶液으로, Zymolyase는 pH7.5에서 20㎛/ml의 濃度의 溶液으로 하여 모두 70分間 處理하는 것으로 決定하였으나 이 정도의 處理時間에서는 protoplast가 극히 不安定하게 되어 regeneration frequency가 떨어지는 것을 確認하였으며, 特히 Zymolyase 處理로 얻어진 protoplast의 regeneration率이 낮은 것은 Zymolyase中에 不純物로 微量 混在한 protease가 protoplast의 노출된 membrane-bound protein을 分解함으로써 protoplast를 破壞시키기 때문인 것으로 추측되었다. 融合實驗에 利用할 수 있을 정도의 regeneration frequency를 얻기 위해서는 Zymolyase를 45分間 處理하여 얻은 protoplast를 1.5%의 polyvinylpyrrolicone이 加해진 OYPD培地에서 重層法으로 展開하여 regeneration시키는 것이 좋은 것으로 판명되었다. As preliminary steps of protoplast fusion between Saccharomyces diastaticus and S. uvarum to develop a fusant of higher ethanol production from starch, characteristics of the two presumptive parent strains, optimal conditions for protoplast preparation and conditions for highrer regeneration frequency were investigated. To determine flavor characteristics of the parent strains, neutral and acidic flavor fractions were extracted from liquids fermented by S. diastaticus and S. diastaticus + S. uvarum with methylene chloride and diethly ether. The liquid by the mixed culture produced more ethly acetate, ethyl undecanoate, n-propanol, n-butanol, lauric acid, caprylic acid and capric acid than that by S. diastaticus. Glucoamylase from S. diastaticus was purified and activity, productivity, and characteristics were determined. Optimum conditions for the enzyme were pH 5.0 and 55℃. The two strains reached logarithmic phase in 7-8h during growth and the generation time was 1.04 in S. diastaticus and 1.38 in S. uvarum. Cell size and DNA content per cell of S. diastaticus were 44.10㎛³and 44.3 fg, and for S. uvarum, 99.67㎛³and 37.6fg. Ethanol productivities of S. diastaticus were 8.9% from 30% glucose and 6.9% from 30% starch and 11.4% from glucose with S. uvarum. Through determination of generation time, cell size, and DNA content per cell, both strains appeared as diploids, and differences in assimilability of melibiose and soluble starch of the two strains were selected as markers to determine the fusant. The optimal condition for protoplast formation was treatment of both strains with 10% ß-glucuronidase at pH 8.0 or 20㎍/ml Zymolyase at pH 7.5 for 70 min. While the regeneration frequencies were very low at 70min exposure to Zymolyase because of the instability of protoplasts, the yeasts treated for 45min were better for regeneration. The regeneration frequencies were also enhanced by 3-6 times when the regeration was carried out with 1.5% polyvinylpyrrolidone which stabilized protoplasts.
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Lee, J.H.,Pascua, P.N.Q.,Decano, A.G.,Kim, S.M.,Park, S.J.,Kwon, H.I.,Kim, E.H.,Kim, Y.I.,Kim, H.,Kim, S.Y.,Song, M.S.,Jang, H.K.,Park, B.K.,Choi, Y.K. Elsevier Science 2015 INFECTION GENETICS AND EVOLUTION Vol.34 No.-
In 2011-2012, contemporary North American-like H3N2 swine influenza viruses (SIVs) possessing the 2009 pandemic H1N1 matrix gene (H3N2pM-like virus) were detected in domestic pigs of South Korea where H1N2 SIV strains are endemic. More recently, we isolated novel reassortant H1N2 SIVs bearing the Eurasian avian-like swine H1-like hemagglutinin and Korean swine H1N2-like neuraminidase in the internal gene backbone of the H3N2pM-like virus. In the present study, we clearly provide evidence on the genetic origins of the novel H1N2 SIVs virus through genetic and phylogenetic analyses. In vitro studies demonstrated that, in comparison with a pre-existing 2012 Korean H1N2 SIV [A/swine/Korea/CY03-1½012 (CY03-1½012)], the 2013 novel reassortant H1N2 isolate [A/swine/Korea/CY0423/2013 (CY0423-12/2013)] replicated more efficiently in differentiated primary human bronchial epithelial cells. The CY0423-12/2013 virus induced higher viral titers than the CY03-1½012 virus in the lungs and nasal turbinates of infected mice and nasal wash samples of ferrets. Moreover, the 2013 H1N2 reassortant, but not the intact 2012 H1N2 virus, was transmissible to naive contact ferrets via respiratory-droplets. Noting that the viral precursors have the ability to infect humans, our findings highlight the potential threat of a novel reassortant H1N2 SIV to public health and underscore the need to further strengthen influenza surveillance strategies worldwide, including swine populations.
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