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한우에 있어서 주간 분만유기에 대한 야간 사료급여의 영향
임석기,연성흠,나승환,강수원,박준철,김현섭,정연후 ( S . K . Im,S . H . Yeon,S . H . Na,S . W . Kang,J . C . Park,H . S . Kim,Y . H . Chung ) 한국축산학회 1997 한국축산학회지 Vol.39 No.3
This study was carried out to investigate the effect of evening feeding to pregnant cows(Hanwoo) on calving time during a day. The investigation revealed that feeding at 17:00∼21:00 resulted in 79.8% of the cows calving between 06:00∼21:00, whereas the tradional 08:00∼09:00 and 17:00v18:00 feeding resulted in 59.3% of them calving during the time period (P$lt;0.001). There were no significant differences in the calving time, parity, feed intakes, calving days, and sex ratios between the two feeding practices.
한우에 있어서 발정주기중 혈중 Progesterone 및 Insulin - like Growth Factor-I 농도의 변화
성환후,우제석,임석기,고응규,백광수,박진기,구용범,이장형 ( H . H . Seong,J . S . Woo,S . K . Im,Y . G . Ko,K . S . Baek,J . K . Park,Y . B . Koo,J . H . Lee ) 한국축산학회 1997 한국축산학회지 Vol.39 No.3
The objective of this study was to determine changes in circulating progesterone and insulinlike growth factor-I(IGF-I) concentrations during estrous cycles in Hanwoo. The serum progesterone and IGF-I concentrations during the estrous cycles in the apparently healthy normal cycles and freemartins of 670 days old were measured with a commercial Radio Immuno Assay(RIA) kit. Serum progesterone concentration was low from Day 1 to 3(about 0.25ng/㎖) and then increased gradually to 9.3ng/㎖ by Day 17, and decreased dramatically by Day 21 of the estrous cycle. However, serum progesterone concentration of freemartins was almost undetectable during the same periods of normal Hanwoo. Serum IGF-I concentrations from Day 3 to 16 of normal estrous cycle were higher than those of early luteal stage( Day 1∼2) and luteal regression stage (Day 19∼20). The lowest serum IGF-I concentration was observed on Day 21. In the freemartin of Hanwoo, serum IGF-I concentrations were 1/4 of those in the normal cycles. It is suggested that the serum progesterone concentration is closely related to the serum IGF-I concentration in Hanwoo.
정희태(H . T . Cheong),임석기(S . K . Im),이준희(J . H . Lee),박춘근(C . K . Park),양부근(B . K . Yang),김정익(C . I . Kim) 한국축산학회 1996 한국축산학회지 Vol.38 No.6
This study was conducted to examine the possibility of cell cycle synchronized nuclear transfer and the effect of the early cell cycle stage of donor embryo on the development of nuclear transplant bovine embryos. Donor embryos were treated with nocodazole, then monitored their nuclear phase and cleavage after release from the nocodazole treatment. Blastomeres cleaved within 3 hr and noncleaved ones were transferred into enucleated oocytes. Some nuclear transplant embryos were fixed to investigate their chromatin structures. Comparatively high proportions(56.0-58.6%) of blastomeres arrested at mitotic phase(M-phase) were obtained following treatments with 0.5∼1.0㎍/㎖ of nocodazole for 16 hr. Cleavage rates of blastomeres within 3 hr after release from nocodazole treatment were average 64%(188/294). After nuclear transfer, 13%(11/85) of embryos were developed to blastocysts following transplantation of blastomeres within 1.5 hr post cleavage(hpc), but 7%(5/74) and 5%(3/63) were developed to blastocysts following transplantation of blastomeres at 3 hpc and noncleaved ones. About 40% of 1.5 hpc nuclear transplant embryos have a single chromatin clump, whereas only 25.0% of 3.0 hpc nuclear transplant eanbryos have a single chromatin clump and the rest have 2 or multi chromatin clumps. From the result of this study, it was suggested that the developmental potential of nuclear transfer bovine embryos may be enhanced with transfer of early cell cycle stage blastomeres immediately after cleavage. It was also suggested that the cell cycle synchronization of bovine embryonic cells at GI phase is very difficult.