Blocking of Cell Proliferation, Cytokines Production and Genes Expression Following Administration of Cordyceps Sinensis in the Bronchoalveolar Lavage Fluids Cells

Kuo, Yuh-Chi,Wu, Chia-Lin,Tasi, Wei-Jern,Wang, Jir-Yenn,Chang, Shi-Chung,Lin, Ching-Yuang,Shiao, Ming-Shi 경희대학교 동서의학연구소 1999 INTERNATIONAL SYMPOSIUM ON EAST-WEST MEDICINE Vol.1999 No.1

Yuh-Chi Kuo¹,Chia-Lin Wn¹,Wei-jern Tasi¹,Jir-Yenn Wang²,Shi-Chung Chang₃,Ching-Yung Lin⁴,and Ming-Shi Shiao⁴¹National Research Institute of Chinese Medicine, ²Department of Biology,Fu Jen University, ³Department of Chest, ⁴Department of Pediatrics, □De[art,amt of Medical Research and Education. Taipei Veterans General Hospital, Taipei, Taiwan Yuh-Chi Kuo¹,Chia-Lin Wn¹,Wei-jern Tasi¹,Jir-Yenn Wang²,Shi-Chung Chang²,Ching-Yung Lin³,and Ming-Shi Shiao⁴.¹National Research Institute of Chinese Medicine, ²Department of Biology,Fu Jen University, ³Department of Chest, ⁴Department of Pediatrics, □De[art,amt of Medical Research and Education. Taipei Veterans General Hospital, Taipei, Taiwan. Blocking of Cell Proliferation, Cytokines Production and Genes Expression Following Administration of Cordyceps sinensis in the Bronchoalveolar Lavage Fluids Cells. Proceedings of international Symposium on East-West Medicine, Seoul. 44-64, 1999.-Cordyceps sinensis is a major parasitic fungus on larva of Lepidoptera. It is one of the well know fungi used in traditional Chinese medicine for treatment asthma, lung and kideney diseases. Many fungi belonging to the genus Cordyceps have been demonstrated to produce natural products with various biological activities. Reported biological activities of genus cordyceps include:(a)inhibition of DNA and RNA synthesis;(b)enhancement of cell differentiation;(c)restruction of cytoskeleton;(d)inhibition of protein kinase activity;(e)antitumor activity on bladder, colon, lung carcinoma as well as fibroblastoma;(f)inhibition of the infection and revers transciptase activity of human immunodeficiency virus type 1; and(g) inhibition of methylation of nucleic acid. Thus, it is suggested that the extracts of Cordyceps sp. Contain antitumor, antiviral, and many other biological activities. In our previous studies, we proved that C. sinensis contains the antitumor and immunomodulatory agents. In the present studies, effects of C. sinensis on bronchoalveolar lavage fluids(BAL)cells were demonstrated. The crude methanolic extracts of C. sinensis were fractionated into 15 fractions by silica gel column chromatography. Effects of various fraction on BAL cells proliferation and lipopolysaccharide(LPS) stimulated interleukin-1β(IL-8),platelet activating factor(PAF), interleukin-6(IL-6), tumor necrosis factor-α(TNF-α),and interleukin-8(IL-8) production on BAL cells were determined. The result indicated that the CS-19-22 fraction suppressed BAL cells proliferation activated by LPS. The median inhibitory concentration(IC50) was 6㎍/ml. The CS-19-22 fraction also decreased IL-1β,PAF,IL-6, TNF-αand IL-8 production.The results of revers transcription-polymerase chain reaction(RT-PCR) demonstrated that the CS-19-22 fraction did not affect IL-1β,PAF,IL-6, TNF-α,and IL-8 mRNAs expression in BAL cells activated by LPS. By contrast, it inhibited the IL-10 mRNA expression but enhanced IFN-γand IL-12 mRNAs expression in activated BAL cells. Moreover, the CS-19-22 fraction blocked PAF-induced platelet aggregation. It is unlikely that cytotoxicity was involved. Because no cell deths were observable. We hypothesize that inhibitory mechanisms of CS-19-22 on BAL cells proliferation may be related to the impairments of gene expression and production of cytokines in BAL cells. The explanation of C. sinensis antiasthma function may involved the following(1)C.sinenesis contained immunomodulatory agents that stimulated IFN-γand IL-12 expression in TH1cells.(2)Enhanced secretion of IFN-γand IL-12 will inhibit the Tγcells immune responses Ex. Decreasing of IL-6 and IL-10 production.(3) The impairments of cytokines production in TH2 cells suppressed th BAL cells proliferation then more cytokines production Ex.IL-1β,PAF,IL-6,TNF-αand IL-8 in activated BAL cells were decreased. (4)The suppression of TH2cells immune responses will inhibit the growth and differentiation of B cells then IgE production will be decreased. (5)Reduced production of IgE will decreased asthma attack occurring in individuals. However, the results of the present study suggested that C. sinensis may also have acted to treat asthma in part by inhibiting BAL cells proliferation and cytokine gene expression and production. Future, experiments with treatment of animals with asthma with C. sinensis will be necessary to defined whether C. sinensis can reduce asthma injury. Plans are underway for the isolation of pure principal growth inhibitors and their mechanisms of action are subjected for further study.

Status, Trend and Strategy on Municipal Wastewater Management in China

Wang Baozhen,Wang Lin,Liu Shuo,Wang Li,Wang Zheng 대전대학교 환경문제연구소 2007 환경문제연구소 논문집 Vol.11 No.-

Recommendations on management of gynecological malignancies during the COVID-19 pandemic: perspectives from Chinese gynecological oncologists

Yingmei Wang,Shiqian Zhang,Lihui Wei,Zhongqiu Lin,Xinyu Wang,Jianliu Wang,Keqin Hua,Manhua Cu,Jiandong Wang,Shixuan Wang,Wen Di,Yudong Wang,Ruifang An,Mingrong Xi,Ruixia Guo,Qi Zhou,Xing Xie,Fengxia X 대한부인종양학회 2020 Journal of Gynecologic Oncology Vol.31 No.4

The outbreak of coronavirus disease 2019 (COVID-19) caused by the severe acute respiratorysyndrome coronavirus 2 has rapidly spread globally. Cancer patients are at a higher risk ofbeing infected with the coronavirus and are more likely to develop severe complications, ascompared to the general population. The increasing spread of COVID-19 presents challengesfor the clinical care of patients with gynecological malignancies. Concerted efforts should beput into managing gynecological malignancies in an orderly manner by strictly implementingthe measures that are specifically developed for controlling the spread of COVID-19. We havedrafted Recommendations on Management of Gynecological Malignancies during the COVID-19 Pandemicbased on our experience on controlling COVID-19 pandemic in China. We recommendthat patients with gynecological malignancies should be managed in hierarchical andindividualized manners in combination with local conditions related to COVID-19. Medicalcare decision should be balanced between controlling COVID-19 pandemic spread and timelydiagnosis and treatment for gynecologic oncology patients.

Sub-100 fs Tm:MgWO₄ laser at 2017 nm mode locked by a graphene saturable absorber

Wang, Yicheng,Chen, Weidong,Mero, Mark,Zhang, Lizhen,Lin, Haifeng,Lin, Zhoubin,Zhang, Ge,Rotermund, Fabian,Cho, Young Jun,Loiko, Pavel OPTICAL SOCIETY OF AMERICA 2017 Optics letters Vol.42 No.16

T Cells Development Is Different between Thymus from Normal and Intrauterine Growth Restricted Pig Fetus at Different Gestational Stage

Lin, Yan,Wang, Junjun,Wang, Xiaoqiu,Wu, Weizong,Lai, Changhua Asian Australasian Association of Animal Productio 2013 Animal Bioscience Vol.26 No.3

This experiment was conducted to evaluate the development of T cells in intrauterine growth retarded (IUGR) piglets at different gestational stages, and tentatively explore the relationship between T cells development and the Notch signaling pathway. A total of 18 crossbred (Landrace${\times}$Large white) primiparous sows were mated at similar weights and estruses and euthanized at d 60, 90 and 110 of gestation with six replicates for each time point. One IUGR and one normal fetus were picked from each litter. The T-cell subsets, mRNA expression of Delta-like1, Delta-like4, Jagged1, and Notch2 genes in the thymus were investigated. Compared to normal piglets, $CD3^+CD4^-CD8^+$ cells in IUGR fetuses at d 90 was 0.13% lower (p<0.05). At d 110 of gestation $CD8^+$ T cells in IUGR fetuses was 0.19% lower (p<0.05). The percentage of $CD8^+$ T cells was 3.14% lower (p<0.05) of the total T cells in IUGR pigs at d 60. The abundance of Notch2 and Delta-like4 mRNA at d 110 was 20.93% higher and 0.77% (p<0.05) lower, and Delta-like1 mRNA at d 90 was 0.19% (p<0.05) higher compared to normal pigs. These results suggested that normal fetuses had a greater proportion of T-cell subsets at earlier gestation periods, and the Notch signaling pathway was likely partially responsible for these differences to some degree.

Power Allocation Optimization and Green Energy Cooperation Strategy for Cellular Networks with Hybrid Energy Supplies

( Lin Wang ),( Xing Zhang ),( Kun Yang ) 한국인터넷정보학회 2016 KSII Transactions on Internet and Information Syst Vol.10 No.9

Energy harvesting is an increasingly attractive source of power for cellular networks, and can be a promising solution for green networks. In this paper, we consider a cellular network with power beacons powering multiple mobile terminals with microwave power transfer in energy beamforming. In this network, the power beacons are powered by grid and renewable energy jointly. We adopt a dual-level control architecture, in which controllers collect information for a core controller, and the core controller has a real-time global view of the network. By implementing the water filling optimized power allocation strategy, the core controller optimizes the energy allocation among mobile terminals within the same cluster. In the proposed green energy cooperation paradigm, power beacons dynamically share their renewable energy by locally injecting/drawing renewable energy into/from other power beacons via the core controller. Then, we propose a new water filling optimized green energy cooperation management strategy, which jointly exploits water filling optimized power allocation strategy and green energy cooperation in cellular networks. Finally, we validate our works by simulations and show that the proposed water filling optimized green energy cooperation management strategy can achieve about 10% gains of MT`s average rate and about 20% reduction of on-grid energy consumption.

Virtualized Cloud Radio Access Network for 5G Transport

Wang, Xinbo,Cavdar, Cicek,Wang, Lin,Tornatore, Massimo,Chung, Hwan Seok,Lee, Han Hyub,Park, Soo Myung,Mukherjee, Biswanath IEEE 2017 IEEE communications magazine Vol.55 No.9

<P>Current radio access networks (RANs) need to evolve to handle diverse service requirements coming from the growing number of connected devices and increasing data rates for the upcoming 5G era. Incremental improvements on traditional distributed RANs cannot satisfy these requirements, so the novel and disruptive concept of a cloud RAN (CRAN) has been proposed to decouple digital units (DUs) and radio units (RUs) of base stations (BSs), and centralize DUs into a central office, where virtualization and cloud computing technologies are leveraged to move DUs into the cloud. However, separating RUs and DUs requires low-latency and high-bandwidth connectivity links, called 'fronthaul,' as opposed to traditional backhaul links. Hence, design of the 5G transport network, that is, the part of the network that carries mobile data traffic between BSs and the core network and data centers, is key to meet the new 5G mobile service requirements and effectively transport the fronthaul traffic. Today, consensus is yet to be achieved on how the fronthaul traffic will be transported between RUs and DUs, and how virtualization of network resources will occur from a radio network segment to the centralized baseband processing units. In this article, we present a new 5G architecture, called virtualized cloud radio access network (V-CRAN), moving toward a cell-less 5G network architecture. We leverage the concept of a virtualized BS (V-BS) that can be optimally formed by exploiting several enabling technologies such as software defined radio (SDR) and coordinated multipoint (CoMP) transmission/reception. A V-BS can be formed on a per-cell basis or per-user basis by allocating virtualized resources on demand. For the fronthaul solution, our approach exploits the passive optical network (PON), where a wavelength can be dynamically assigned and shared to form a virtualized passive optical network (VPON). Several use cases of the V-CRAN are presented to show how network architecture evolution can enhance system throughput, energy efficiency, and mobility management.</P>

New damage localization indicator based on curvature for single-span beams

Wang, Yi-lin Techno-Press 2014 Structural Engineering and Mechanics, An Int'l Jou Vol.51 No.6

Most existing damage detection methods based on curvature should investigate the indicator value changes between the intact and damaged state. However, the 'footprint' in the intact state is usually not available for most actual beams. Therefore, a new damage localization indicator called Difference of Nearby Difference Curvature Indicator (DNDCI) was introduced for single-span beams. This indicator does not require prior information of the intact beam and is sensitive to the damage occurs on or nearby the measuring points. Practical and detailed application method of DNDCI has been proposed. Besides the idealized simply supported beams, it was validated by example that DNDCI is also applicable to the actual single-span beams supported by bearings.

Genetic Diversity of Chinese Indigenous Pig Breeds in Shandong Province Using Microsatellite Markers

Wang, J.Y.,Guo, J.F.,Zhang, Q.,Hu, H.M.,Lin, H.C.,Wang, Cheng,Zhang, Yin,Wu, Y. Asian Australasian Association of Animal Productio 2011 Animal Bioscience Vol.24 No.1

To investigate the genetic diversity of six Chinese indigenous pig breeds in Shandong province (Laiwu Black, Dapulian Black, Licha Black, Yantai Black, Yimeng Black and Wulian Black), explain their genetic relationship and assess their integrity and degree of admixture with three Western commercial breeds (Landrace, Yorkshire and Duroc), 303 individuals from these breeds were genotyped for 26 microsatellite markers. In general, high genetic diversity (observed heterozygosity ranging from 0.5495 to 0.7746) and large breed differentiation ($F_{ST}$ = 0.188) were observed. The indigenous pig breeds in Shandong exhibited consistently higher levels of genetic diversity than the three Western breeds. However, compared with the Western breeds, which have an $F_{ST}$ value of 0.252, the indigenous breeds in Shandong have smaller $F_{ST}$ value of 0.145. The analysis of breed relationship indicated that the six indigenous breeds are classified into two groups. One includes four breeds, Licha, Yantai, Yimeng and Wulian, which have experienced large gene introgression of the Western breeds through progressive crossbreeding as well as gene flow among themselves. The other includes Laiwu and Dapulian, which are less influenced by the Western breeds and other indigenous breeds in Shandong in the recent past. The results show that some measures must be taken to effectively protect these indigenous pig breeds in Shandong.

A new conjugate gradient algorithm for solving dynamic load identification

Lin J. Wang,Qi C. Deng,You X. Xie 국제구조공학회 2017 Structural Engineering and Mechanics, An Int'l Jou Vol.64 No.2

In this paper, we propose a new conjugate gradient method which possesses the global convergence and apply it to solve inverse problems of the dynamic loads identification. Moreover, we strictly prove the stability and convergence of the proposed method. Two engineering numerical examples are presented to demonstrate the effectiveness and speediness of the present method which is superior to the Landweber iteration method. The results of numerical simulations indicate that the proposed method is stable and effective in solving the multi-source dynamic loads identification problems of practical engineering.