Recommendations on management of gynecological malignancies during the COVID-19 pandemic: perspectives from Chinese gynecological oncologists

Yingmei Wang,Shiqian Zhang,Lihui Wei,Zhongqiu Lin,Xinyu Wang,Jianliu Wang,Keqin Hua,Manhua Cu,Jiandong Wang,Shixuan Wang,Wen Di,Yudong Wang,Ruifang An,Mingrong Xi,Ruixia Guo,Qi Zhou,Xing Xie,Fengxia X 대한부인종양학회 2020 Journal of Gynecologic Oncology Vol.31 No.4

The outbreak of coronavirus disease 2019 (COVID-19) caused by the severe acute respiratorysyndrome coronavirus 2 has rapidly spread globally. Cancer patients are at a higher risk ofbeing infected with the coronavirus and are more likely to develop severe complications, ascompared to the general population. The increasing spread of COVID-19 presents challengesfor the clinical care of patients with gynecological malignancies. Concerted efforts should beput into managing gynecological malignancies in an orderly manner by strictly implementingthe measures that are specifically developed for controlling the spread of COVID-19. We havedrafted Recommendations on Management of Gynecological Malignancies during the COVID-19 Pandemicbased on our experience on controlling COVID-19 pandemic in China. We recommendthat patients with gynecological malignancies should be managed in hierarchical andindividualized manners in combination with local conditions related to COVID-19. Medicalcare decision should be balanced between controlling COVID-19 pandemic spread and timelydiagnosis and treatment for gynecologic oncology patients.

Middleware Technology Research and Interface Design Based on Internet of Things

Yingmei Wang 보안공학연구지원센터 2015 International Journal of Smart Home Vol.9 No.12

With the advent of Internet of Things (IoT) technology, communications between physical objects and humans become realistic, and smart devices can be monitored and controlled remotely. It promotes the development of smart home, smart city and even smart planet, which greatly improves the quality and efficiency of human life. As a kind of common services, middleware technology plays an importance role in IoT system. This paper firstly introduces the research background of IoT and middleware technology. Then three main middleware access scenes are analyzed in IoT system, which is determined by the sensing device functions. Lastly, this paper defines the middleware interface, and proposes the interface design method. The middleware interface is classified into three categories: device side interface, application side interface and middleware management interface, in which device side interface defines the data transmission method between the device layer and the platform layer; application side interface defines the middleware opening resources and services between the application layer and the platform layer; and middleware management interface is mainly for devices and middleware management functions. It indicates the message interaction process, and separates different message information, thus improving the data transmission efficiency and enhancing the services open ability of IoT system.

An adaptive nonlocal filtering for low-dose CT in both image and projection domains

Wang, Yingmei,Fu, Shujun,Li, Wanlong,Zhang, Caiming Society for Computational Design and Engineering 2015 Journal of computational design and engineering Vol.2 No.2

An important problem in low-dose CT is the image quality degradation caused by photon starvation. There are a lot of algorithms in sinogram domain or image domain to solve this problem. In view of strong self-similarity contained in the special sinusoid-like strip data in the sinogram space, we propose a novel non-local filtering, whose average weights are related to both the image FBP (filtered backprojection) reconstructed from restored sinogram data and the image directly FBP reconstructed from noisy sinogram data. In the process of sinogram restoration, we apply a non-local method with smoothness parameters adjusted adaptively to the variance of noisy sinogram data, which makes the method much effective for noise reduction in sinogram domain. Simulation experiments show that our proposed method by filtering in both image and projection domains has a better performance in noise reduction and details preservation in reconstructed images.

Study of Data Stream Clustering Based on MSF

Yingmei Li,Min Li,Jingbo Shao,Gaoyang Wang 보안공학연구지원센터 2015 International Journal of Database Theory and Appli Vol.8 No.1

Nowadays with the rapid development of wireless sensor networks, and network traffic monitoring, stream data gradually becomes one of the most popular data models. Stream data is different from the traditional static data. Clustering analysis is an important technology for data mining, so that many researchers pay their attention to the clustering of stream data. In this paper, MSFS algorithm is proposed. By means of the experimental verification analysis, based on biologically inspired computational model, higher clustering purity on both the real dataset and the simulation datasets existence is demonstrated for the proposed algorithm. In other words, the cluster result of MSFS algorithm is advantageous over previous method.

Site-specific ubiquitination of VDAC1 restricts its oligomerization and mitochondrial DNA release in liver fibrosis

Wu Ne N.,Wang Lifeng,Wang Lu,Xu Xihui,Lopaschuk Gary D.,Zhang Yingmei,Ren Jun 생화학분자생물학회 2023 Experimental and molecular medicine Vol.55 No.-

Mitochondrial DNA (mtDNA) released through protein oligomers, such as voltage-dependent anion channel 1 (VDAC1), triggers innate immune activation and thus contributes to liver fibrosis. Here, we investigated the role of Parkin, an important regulator of mitochondria, and its regulation of VDAC1-mediated mtDNA release in liver fibrosis. The circulating mitochondrial DNA (mtDNA) and protein levels of liver Parkin and VDAC1 were upregulated in patients with liver fibrosis. A 4-week CCl4 challenge induced release of mtDNA, activation of STING signaling, a decline in autophagy, and apoptosis in mouse livers, and the knockout of Parkin aggravated these effects. In addition, Parkin reduced mtDNA release and prevented VDAC1 oligomerization in a manner dependent on its E3 activity in hepatocytes. We found that site-specific ubiquitination of VDAC1 at lysine 53 by Parkin interrupted VDAC1 oligomerization and prevented mtDNA release into the cytoplasm under stress. The ubiquitination-defective VDAC1 K53R mutant predominantly formed oligomers that resisted suppression by Parkin. Hepatocytes expressing VDAC1 K53R exhibited mtDNA release and thus activated the STING signaling pathway in hepatic stellate cells, and this effect could not be abolished by Parkin. We propose that the ubiquitination of VDAC1 at a specific site by Parkin confers protection against liver fibrosis by interrupting VDAC1 oligomerization and mtDNA release.

Identification and Characterization of CMTM4, a Novel Gene with Inhibitory Effects on HeLa Cell Growth through Inducing G2/M Phase Accumulation

Plate, Markus,Li, Ting,Wang, Yu,Mo, Xiaoning,Zhang, Yingmei,Ma, Dalong,Han, Wenling Korean Society for Molecular and Cellular Biology 2010 Molecules and cells Vol.29 No.4

Human CMTM is a novel gene family consisting of CKLF and CMTM1-8. CMTM4 is the most conserved gene and has three RNA splicing forms designated as CMTM4-v1, -v2 and -v3, but in many types of tissue and cell lines, only CMTM4-v1 and -v2 could be detected. CMTM4-v2 is the full length cDNA product, which has been highly conserved during e volution. CMTM4-v1 and -v2 a re broadly expressed in normal types of tissue. They are distributed on the cell membrane and across the cytoplasm in a speckled pattern. Overexpression of CMTM4-v1 and -v2 can inhibit HeLa cell growth via G2/M phase accumulation without inducing apoptosis. Therefore, CMTM4 might be an important gene involved in cell growth and cell cycle regulation.

Characterization and expression analysis of inositolphosphorylceramide synthase family genes in rice (Oryza sativa L.)

Pengfei Liao,Jinqiu Huang,Puguo Tong,Wang Nie,Xin Yan,Yingmei Feng,Hui Peng,Xiaojue Peng,Shao-Bo Li 한국유전학회 2017 Genes & Genomics Vol.39 No.5

Genes encoding inositolphosphorylceramide synthase (IPCS), which play essential roles in sphingolipid biosynthesis, have been known to be widely distributed in plants. However, reports on identification, expression, and function analysis of plant IPCS family genes are very limited. In this study, a total of three OsIPCS genes were identified in the rice database, and their full-length coding regions were amplified by RT-PCR. These genes had 12–13 exons, and they encoded proteins of 313–326 amino acids with molecular mass of 35.8–37.6 kDa and isoelectric point of 10.99–12.61. Importantly, two typical and conserved motifs (D3 and D4) in plants were found in all the three putative OsIPCS proteins, indicating high similarity of OsIPCSs to the other plant IPCS proteins, which were confirmed by phylogenetic analysis. The transcripts of OsIPCS genes appeared in different rice organs including seedling roots, stems and leaves, and young panicles, but each gene showed a unique organ-specific expression pattern. Meanwhile, we found that all the three OsIPCS genes showed responses to stresses like drought, cold and salt, but their expression patterns were also to some extent different under stress treatments. Especially, under cold stress, OsIPCS1 and OsIPCS2 were significantly up-regulated in roots and stems but down-regulated in leaves, while OsIPCS3 was significantly up-regulated in all the measured organs. Actually, putative abiotic-stress regulatory elements were identified in the promoters of these genes. Our data suggest that OsIPCS genes should play important roles during rice growth and adversity adaptation.

Identification and Characterization of CMTM4, a Novel Gene with Inhibitory Effects on HeLa Cell Growth through Inducing G2/M Phase Accumulation

Markus Plate,Ting Li,Yu Wang,Xiaoning Mo,Yingmei Zhang,Dalong Ma,Wenling Han 한국분자세포생물학회 2010 Molecules and cells Vol.29 No.4

Human CMTM is a novel gene family consisting of CKLF and CMTM1-8. CMTM4 is the most conserved gene and has three RNA splicing forms designated as CMTM4-v1, -v2 and -v3, but in many types of tissue and cell lines, only CMTM4-v1 and -v2 could be detected. CMTM4-v2 is the full length cDNA product, which has been highly conserved during evolution. CMTM4-v1 and -v2 are broadly ex-pressed in normal types of tissue. They are distributed on the cell membrane and across the cytoplasm in a speckled pattern. Overexpression of CMTM4-v1 and -v2 can inhibit HeLa cell growth via G2/M phase accumulation without inducing apoptosis. Therefore, CMTM4 might be an impor-tant gene involved in cell growth and cell cycle regulation.

Potential Vaccine Targets against Rabbit Coccidiosis by Immunoproteomic Analysis

Hongyan Song,Ronglian Dong,Baofeng Qiu,Jin Jing,Shunxing Zhu,Chun Liu,Yingmei Jiang,Shengcun Wang,Jin Miao,Yixiang Shao 대한기생충학열대의학회 2017 The Korean Journal of Parasitology Vol.55 No.1

Ginsenoside 20(S)-Rg3 reduces KIF20A expression and promotes CDC25A proteasomal degradation in epithelial ovarian cancer

Rong Zhang,Lei Li,Huihui Li,Hansong Bai,Yuping Suo,Ju Cui,Yingmei Wang 고려인삼학회 2024 Journal of Ginseng Research Vol.48 No.1

Background: Ginsenoside 20(S)-Rg3 shows promising tumor-suppressive effects in ovarian cancer viainhibiting NF-kB signaling. This study aimed to explore the downstream tumor suppressive mechanismsof ginsenoside Rg3 via this signaling pathway. Materials and methods: A systematical screening was applied to examine the expression profile of 41kinesin family member genes in ovarian cancer. The regulatory effect of ginsenoside Rg3 on KIF20Aexpression was studied. In addition, we explored interacting proteins of KIF20A and their molecularregulations in ovarian cancer. RNA-seq data from The Cancer Genome Atlas (TCGA) was used for bioinformaticanalysis. Epithelial ovarian cancer cell lines SKOV3 and A2780 were used as in vitro and in vivocell models. Commercial human ovarian cancer tissue arrays were used for immunohistochemistrystaining. Results: KIF20A is a biomarker of poor prognosis among the kinesin genes. It promotes ovarian cancercell growth in vitro and in vivo. Ginsenoside Rg3 can suppress the transcription of KIF20A. GST pull-downand co-immunoprecipitation (IP) assays confirmed that KIF20A physically interacts with BTRC (b-TrCP1),a substrate recognition subunit for SCFb TrCP E3 ubiquitin ligase. In vitro ubiquitination and cycloheximide(CHX) chase assays showed that via interacting with BTRC, KIF20A reduces BTRC-mediated CDC25Apoly-ubiquitination and enhances its stability. Ginsenoside Rg3 treatment partly abrogates KIF20Aoverexpression-induced CDC25A upregulation. Conclusion: This study revealed a novel anti-tumor mechanism of ginsenoside Rg3. It can inhibit KIF20Atranscription and promote CDC25A proteasomal degradation in epithelial ovarian cancer.