녹동균 세포외막 단백질 백신 CFC-1-101의 안정성 및 면역원성 검토 : 임상 제 Ⅰ/Ⅱa상 시험

장인진,김익상,유경상,임동석,김형기,신상구,장우현,박완제,이나경,정상보,안동호,조양제,안보영,이윤하,김영지,남성우,김현수 대한감염학회 1998 감염 Vol.30 No.3

목적 : 제일제당에서는 녹농균의 세포외막 단밸질을 유효성분으로 하는 백신인 CFC-101을 개발하였으며, 동물시험에서 이 백신의 안전성과 유효성을 입증하였다. 본 연구에서는 이 녹농균 백신의 인체에 대한 안전성과 면역원성을 평가하는 동시에 인체 접종시의 최적 투여 용량을 결정하기 위하여 제 I/Ⅱa상 임상시험을 수행하였다. 방법 : 건강한 성인 남자를 피험자로 선별하여 각 용량군에 백신투여자 6명, 위약투여자 2명을 배정하였다. 백신 투여군은 0.25mg, 0.5mg 또는 1.0mg 용량의 녹농균 백신을 7일 간격으로 3회에 걸쳐 근육주사 하였으며, 위약 투여군에게는 세포외막 단백질을 제외한 동일한 성분을 투여하였다. 백신접종 후 국소적 또는 전신적인 반응의 발생여부를 관찰하고, 혈액시료를 체취하여 백신의 역가와 유효성을 검정하였다. 결과 : 녹농균 백신 CFC-101은 모든 접종자에서 양호한 내약성을 보였다. 또한 0.5mg 과 1.0mg 백신 투여군에서는 100%의 항체양전율을 나타내었다. 생성된 항체는 녹농균 세포외막단백질에 특이성을 보였고, 녹농균 감염에 대해 방어효능이 있었다. 결론 : 이와같은 결과로부터 이 녹농균 백신은 인체에 안전하게 투여할 수 있으며, 높은 항체 생성능으로 감염방어 효능을 보이고 0.5mg과 1.0mg이 최적용량인 것으로 판단되었다. Background : We developed a Pseudomonas aeruginosa outer membrane protein(OMP) vaccine CFC-101, and the prophylactic efficacy of which has been demonstrated in animal models. In order to evaluate the safety and immunogenicity of the P. aeruginosa vaccine, we carried out a phase I/Ⅱa clinical trial in healthy male volunteers. Methods : Groups of eight volunteers, including two placebo subjects, were vaccinated intramuscularly with three doses of 0.25, 0.5 or 1.0 mg of the vaccine at one week intervals. Sings of systemic and local reactions observed after vaccination were recorded for each vaccinee for 5 days. Physical examinations were performed on days 0, 1, 7, 8, 14, 15, 21, and 42, and clinical laboratory tests were done on days 0, 3, and 21. Blood samples for assay of serum antibody levels were obtained up to 42 days after the first vaccination. Results : The vaccine was generally well tolerated by all vaccinees, showing no significant side effects. In the three dosage groups, all vaccinees, except one receiving the 0.25 mg dose, showed significant elevation in serum IgG antibody titers against the vaccine proteins, indicating 100% seroconversion in 0.5 and 1.0 mg groups. The human antibodies induced by the vaccine were specific for P. aeruginosa OMPs, as confirmed by western blot analysis and immunoprecipitation assays. The capacity of the human antisera to enhance opsonophagocytic killing activity by polymorphonuclear leukocytes and to confer protection against P. aeruginosa infections indicates that the antibodies elicited by the vaccine have protective efficacy. Conclusion : We conclude that the P. aeruginosa OMP vaccine is safe and effective for human use and its optimal dose to be 0.5 or 1.0 mg.

A Polyacetylene and Flavonoids from Cirsium rhinoceros

Yim, Soon-Ho,Kim, Hyun-Jung,Lee, Ik-Soo The Pharmaceutical Society of Korea 2003 Archives of Pharmacal Research Vol.26 No.2

Cirsium rhinoceros Nakai (Compositae) is a herbaceous perennial native to Korea, whole plant of which has been used in folklore medicine. C. rhinoceros was extracted by a standard extraction procedure. Its n-hexane, $CHCl_3$ and n-BuOH extracts were fractionated by column chromatography to provide a polyacetylene, a coumarin and five flavonoids. Ciryneol C, scopoletin, acacetin, cirsimarin were isolated for the first time from this plant.

Treatment of Scalp Arteriovenous Malformation

Jung, Sung-Hoon,Yim, Man-Bin,Lee, Chang-Young,Song, Dal-Won,Kim, Il-Man,Son, Eun-Ik The Korean Neurosurgical Society 2005 Journal of Korean neurosurgical society Vol.38 No.4

Objective : The object of this study is to present the treatment experience of the 6cases of scalp arteriovenous malformations[AVMs] focus on treatment strategy. Methods : Six patients with scalp AVM were treated during past 12years. We analysis the clinical characteristics of the lesions, treatment methods and management outcomes. Results : The lesions were located on temporal in 2patients, parietal in 2patients, frontal and occipital area in each one. Four of six patients had a trauma history on scalp. The presenting symptoms were progressive enlarged pulsating mass with or without bruit. Four of the six lesions had the large fistula in the lesion. Two patients were treated with surgical resection alone, three patients with proximal feeding artery balloon[s] occlusion followed by surgical resection, and one patient with coil embolization through trans-venous route alone. We obtained good results in all patients. Conclusion : Most of scalp AVM can be completely cured by Judicious selection and a combination of treatment modalities, i.e., surgery only, or embolization only, or embolization plus surgical therapy. Although embolization became a primary therapy for this sort of scalp AVM recently, the selection of treatment modality should be chose based on the size, angioarchitecture, and clinical presentations of the lesion.

Microbial Metabolism of the Environmental Estrogen Bisphenol A

Yim, Soon-Ho,Kim, Hyun-Jung,Lee, Ik-Soo The Pharmaceutical Society of Korea 2003 Archives of Pharmacal Research Vol.26 No.10

Preliminary microbial metabolism studies of bisphenol A (BPA) (1) on twenty six microorganisms have shown that Aspergillus fumigatus is capable of metabolizing BPA. Scale-up fermentation of 1 with A. fumigatus gave a metabolite (2) and its structure was established as bisphenol $A-O-{\beta}-D-glucopyranoside$ (BPAG) based on spectroscopic analyses.

Orbitozygomatic Approach in Early Surgery for Cerebral Aneurysms

Yim, Man-Bin,Lee, Jang-Chull,Son, Eun-Ik,Kim, Dong-Won,Kim, In Hong,Bae, Jung-In 啓明大學校 醫科大學 1994 계명의대학술지 Vol.13 No.2

뇌동맥류를 조기수술시 지연수술시와 비교하여 수술수기상 문제점이 되는 것중 중요한 하나는 뇌의 팽창 때문에 안전한 대뇌견인이 어렵다는 점이다. 따라서 두개저골편을 광범위하게하고 대뇌견인을 최소화하는 방법을 고려해 볼 수 있다. 저자들은 과거 제한된 예들에서 이러한 방법을 시행하고 3년이상 추적관찰한 후 장단점, 합병증 및 결과를 분석하고 수술방법 및 적응증에 대하여 기술하고자 한다. 재료는 1982년 9월부터 1994년 2월까지 본원에서 수술한 640례의 뇌동맥류 환자중 1990년 6월부터 9월까지 뇌지주막하 출혈후 4일내에 두개저골편을 광범위하게 하고 뇌동맥류를 결찰한 14례를 대상으로 하였다. 수술방법은 전두-안와-협골-측추골을 연결하는 첫 골편을 만들고 두번째로 시신경관과 상안와열을 연결하는 골편을 만들었으며, 재건은 첫 골편과 두번째 골편을 철사로 연결후 두개골의 miniplate로 고정하였다. 결과는 12례에서 신경학적 장애없이 회복하였으며, 2례가 뇌혈관 연축에 기인하여 사망하였다. 합병증으로 수술중 2례가 경미한 전두엽 손상이 골편제거중 발생하였으며, 5례가 수술직후에 심한 안와주위 부종과 안검하수가 관찰되었으나 자연적으로 소실하였고, 추적관찰중 1례가 수술부위의 협골부위에 감각소실, 1례가 측두근 위축소견을 보였다. 장점은 수술중 대뇌견인을 최소화할 수 있었으며, 중대뇌동맥류에서는 M1, 전교통동맥류에서는 양측 A1 및 A2를 확인하는 것이 용이하였다. 단점으로는 수술시간이 길었고, 수술중 혈액 손실이 많았으며 고도의 수술수기가 요구되었다. 결론은 anterior circulation에 발생한 뇌동맥류의 조기수술에 이 방법을 일률적으로 적용하는 것은 무리이나 뇌부종으로 뇌실위축을 보이는 예들에서는 유용할 것으로 생각되었고, 기저동맥 상부에 발생한 뇌동맥류를 조기수술시에는 절대적으로 유용할 것으로 생각되었다. 아울러 안와동맥 주위에 발생한 뇌동맥류, 높이 위치한 기저동맥 동맥류에서도 수술시기에 관계없이 이 방법이 유용할 것으로 추정되었고 또한 해면정맥동내의 수술 및 두개저에 발생한 종양등의 수술시에도 이 방법은 많은 도움을 줄것으로 사료되었다. We presented the orbitozygomatic approach in early surgery for the intracranial aneuryams. This approach provided excellent wide exposure, minimized brain retraction under the brain swelling state resulting from recent SAH. However, this approach also has several disadvantages such as long operation time, required more surgical skill, and large amounts of blood loss during the operation. We conclude that this approach should be used in early surgery for anterior circulation aneurysms accompanied with brain edema and small ventricle, and upper basilar trunk aneurysms. However, we think this approach has significant advantages for surgery of ophthalmic, paraclinoid, intracavernous sinus and high positioned basilar top aneurysms, and skull base tumors.

Structure-Guided Identification of Novel Phenolic and Phenolic Amide Allosides from the Rhizomes of Cimicifuga heracleifolia

Yim, Soon-Ho,Kim, Hyun-Jung,Jeong, Na-Ri,Park, Ki-Deok,Lee, Young-Ju,Cho, Sung-Dong,Lee, Ik-Soo Korean Chemical Society 2012 Bulletin of the Korean Chemical Society Vol.33 No.4

Two phenolic allopyranosides and two phenolic amide allopyranosides, along with eight known phenolic compounds, including cimicifugic acids, shomaside B, fukiic acid, isoferulic acid, and piscidic acid, were isolated from the n-butanolic extract of rhizomes of Cimicifuga heracleifolia. On-line spectroscopic data for UV, NMR, and MS from a combination of LC-NMR and LC-MS techniques directly and rapidly provided sufficient structural information to identify and confirm all the structures of major phenolic compounds in the extract, in addition to their HPLC profiles. This combined analytic information was then used as a dereplication tool for structure-guided screening in order to isolate unknown phenolic compounds in the extract. Successive fractionation and purification using semi-preparative HPLC acquired four unknown allopyranosides, and their structures were identified as cis-ferulic acid 4-O-${\beta}$-D-allopyranoside, trans-ferulic acid 4-O-${\beta}$-D-allopyranoside, trans-feruloyltyramine 4-O-${\beta}$-D-allopyranoside, and trans-feruloyl-(3-O-methyl)dopamine 4-O-${\beta}$-D-allopyranoside, based on a subsequent spectroscopic interpretation.

기저동맥 분기부 동맥류와 기저동맥-상소뇌동맥 분기부 동맥류에 대한 측두엽하 접근

임만빈,김상열,손은익,김동원,이정교,김인홍,배정인 대한신경외과학회 1988 Journal of Korean neurosurgical society Vol.17 No.6

The pterional(Transsylvian) and subtemporal approaches are main surgical route for direct clipping of basilar bifurcation and basilar-superior cerebellar artery aneurysms. In general, the pterional approach has the advantage for high placed aneurysm and the subtemporal approach has the advantage for low placed and posterior directed aneurysm of basilar bifurcation. The authers has been performed surgery for 5 cases of basilar bifurcation and basilar-superior cerebellar artery aneurysms through subtemporal route during 1.5 years with good results in 3 cases, fair result in 1 case, and death in 1 case. We described about this 5 cases detaily and discussed the subtemporal approach for these lesions.

Measurments of the Heart in Healthy Korean Adults

Yim, Chung Ik,Park, Seog Hee,Kim, Jung Jin,Bahk, Yong Whee CATHOLIC MEDICAL CENTER 1977 Bulletin of the Clinical Research Institute Vol.5 No.1

1. The mean of the transverse cardiac diameter is 12.6±1.2 cm in the male and 11,8±1.0 cm in the female when pericardial fat pad is included. The mean of the transverse cardiac diameter is 12.5±1.1 cm in the male and 11.6±1.0 cm in the female when pericardial fat pad is excluded. The difference is significant (p<0.05). 2. When the pericardial fat is included in the transverse cardiac diameter, the upper limits of the transverse cardiac diameter is 15.0 cm in the normal male and 13.9 cm in the female. Therefore a transverse cardiac diameter larger than 15.5 cm in the male and 14.4 cm in the female may be considered abnormal. 3. When pericardial fat is excluded, the upper limit of the transverse cardiac diameter is 14.8 cm in the normal male and 13.5 cm in the female. Therefore a transverse cardiac diameter larger than 15.3 cm in the male and 14.0 cm in the female may be considered abnormal. 4. The mean of the cardio-thoracic ratio is 43.2±3.9% in the male and 44.4±3.5% in the femal when pericardial fat is excluded.

Microstructure and Mechanical Property Changes of Unidirectional and Plain Woven CF/Mg Composite Laminates after Corrosion

Park, Ik Min,Yim, Shi On,Lee, Jung Moo,Lee, Sang Kwan,Park, Yong Ho The Korean Institute of Metals and Materials 2012 대한금속·재료학회지 Vol.50 No.9

호중구에 의한 EDTA 의존성 혈소판 탐식현상을 보인 균상식육종 1예

하경임,김문연,김정란,서무규,권소영,조한익 대한임상병리학회 1998 대한임상병리학회지 Vol.18 No.1